Adaptational response in transcription factors during development of myocardial hypertrophy.


:Cardiac hypertrophy is characterized, among others, by the molecular events which selectively activate the expression of genes for contractile proteins within individual myocardial cells. As such, myosin light chain 2 (MLC-2), which is upregulated in the hypertrophic state in both rat and human, serves as a marker for hypertrophy. In an attempt to investigate the gene regulatory mechanisms of this phenomenon, we tested the hypothesis that certain transcription factors are directly involved in the development of cardiac hypertrophy by demonstrating the presence of cardiac tissue-specific regulatory elements in the 5'-flanking region of the MLC-2 promoter and testing them in the gel mobility shift assay for their binding activity to nuclear proteins from hypertrophied and normal cardiac tissue. In nuclear extracts from the ventricular tissues of the spontaneously hypertensive rat (SHR), distinctive changes in two families of activator proteins, the A/T-rich DNA-binding transcription factors, myocyte enhancer factor (MEF-2) and CArG-binding factor, manifested in a developmentally dictated manner paralleling the evolution of cardiac hypertrophy in these animals. Extracts isolated from brains and skeletal muscle tissues from the same animals did not exhibit the changes in binding activity. Also, the changes were not apparent when a distal negative regulatory element (CSS), which confers cardiac-specific expression, was tested in gel mobility shift assays. The ubiquitous TATA-binding proteins remained unchanged in comparing SHR with the control strain WKY in the same assay. These data support the notion that the expression of specific transcription factors is modulated in response to hypertrophy related signals which execute changes at the gene level effecting the enrichment of certain contractile proteins in an effort discrete and estranged from the basal transcription machinery.


J Mol Cell Cardiol


Doud SK,Pan LX,Carleton S,Marmorstein S,Siddiqui MA




Has Abstract


1995-10-01 00:00:00












  • Extracellular matrix alterations in hypertensive vascular remodeling.

    abstract::Vascular cells are very sensitive to their hemodynamic environment. Any change in blood pressure or blood flow can be sensed by endothelial and vascular smooth muscle cells and ultimately results in structural modifications within the vascular wall that accommodate the new conditions. In the case of hypertension, the ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Lemarié CA,Tharaux PL,Lehoux S

    更新日期:2010-03-01 00:00:00

  • Dual effect of the heart-targeting cytokine cardiotrophin-1 on glucose transport in cardiomyocytes.

    abstract::Cardiotrophin-1 (CT-1) is a heart-targeting cytokine that is increased in the metabolic syndrome due to overexpression in the adipocytes. The effects of CT-1 on cardiomyocyte substrate metabolism remain unknown. We therefore determined the effects of CT-1 on basal and stimulated glucose transport in cardiomyocytes exp...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Asrih M,Gardier S,Papageorgiou I,Montessuit C

    更新日期:2013-03-01 00:00:00

  • Function, subcellular localization and assembly of a novel mutation of KCNJ2 in Andersen's syndrome.

    abstract::Andersen's syndrome (AS) (which is characterized by periodic paralysis, cardiac arrhythmias and dysmorphic features), a hereditary disease, and missense mutations of KCNJ2 (which encodes an inward rectifying potassium channel) have been reported recently. We performed clinical and molecular analyses of a patient with ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Hosaka Y,Hanawa H,Washizuka T,Chinushi M,Yamashita F,Yoshida T,Komura S,Watanabe H,Aizawa Y

    更新日期:2003-04-01 00:00:00

  • Thymosin beta 4 treatment after myocardial infarction does not reprogram epicardial cells into cardiomyocytes.

    abstract::Myocardial infarction (MI) is one of the leading causes of morbidity and mortality world-wide. Whether endogenous repair and regenerative ability could be augmented by drug administration is an important issue for generation of novel therapeutic approach. Recently it was reported that in mice pretreated with thymosin ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Zhou B,Honor LB,Ma Q,Oh JH,Lin RZ,Melero-Martin JM,von Gise A,Zhou P,Hu T,He L,Wu KH,Zhang H,Zhang Y,Pu WT

    更新日期:2012-01-01 00:00:00

  • Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles.

    abstract::Monoclonal and polyclonal antibodies to the major sarcoplasmic reticulum proteins of rabbit skeletal and canine cardiac muscle have been used to identify and characterize the corresponding components of human cardiac sarcoplasmic reticulum. The Ca2(+)-transporting ATPase of human cardiac sarcoplasmic reticulum was ide...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Movsesian MA,Leveille C,Krall J,Colyer J,Wang JH,Campbell KP

    更新日期:1990-12-01 00:00:00

  • Fibronectin expression during physiological and pathological cardiac growth.

    abstract::Fibronectin (FN) is a dimeric glycoprotein found in the extracellular matrix of most tissues that serves as a bridge between cells and the interstitial collagen meshwork and influences diverse processes including cell growth, adhesion, migration, and wound repair. Multiple FN forms arise by the alternative splicing of...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Farhadian F,Contard F,Corbier A,Barrieux A,Rappaport L,Samuel JL

    更新日期:1995-04-01 00:00:00

  • NHE-1 inhibition improves impaired mitochondrial permeability transition and respiratory function during postinfarction remodelling in the rat.

    abstract::The mechanism by which Na+-H+ exchange (NHE) inhibition results in attenuation and reversal of postinfarction remodelling and heart failure remains controversial. In this study, we investigated the possible contribution of mitochondrial involvement by determining the effect of the NHE-1-specific inhibitor EMD-87580 (E...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Javadov S,Huang C,Kirshenbaum L,Karmazyn M

    更新日期:2005-01-01 00:00:00

  • Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts.

    abstract::Isolated and perfused rat hearts were examined by 2-dimensional gel electrophoresis and liquid scintillation counting to determine the effect of ischemia or perfusion temperature on protein synthesis. Isolated hearts were subjected to ischemia at either 4 degrees, 20 degrees, or 30 degrees C and then perfused at 37 de...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Currie RW

    更新日期:1987-08-01 00:00:00

  • δPKC interaction with the d subunit of F1Fo ATP synthase impairs energetics and exacerbates ischemia/reperfusion injury in isolated rat hearts.

    abstract::Previously, we demonstrated protection against hypoxic injury in neonatal cardiac myocytes and reduced release of cardiac troponin I from perfused rat hearts by a novel peptide inhibitor [NH2-YGRKKRRQRRRMLATRALSLIGKRAISTSVCAGRKLALKTIDWVSFDYKDDDDK-] of the delta protein kinase C (δPKC) interaction with the "d" subunit ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Walker M,Caldwell RW,Yoon Y,Nguyen TT,Johnson JA

    更新日期:2015-12-01 00:00:00

  • Mitochondrial adenine nucleotide transport and cardioprotection.

    abstract::Mitochondria are highly metabolically active cell organelles that not only act as the powerhouse of the cell by supplying energy through ATP production, but also play a destructive role by initiating cell death pathways. Growing evidence recognizes that mitochondrial dysfunction is one of the major causes of cardiovas...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Das S,Steenbergen C

    更新日期:2012-02-01 00:00:00

  • Mechanical strain induces a pro-fibrotic phenotype in human mitral valvular interstitial cells through RhoC/ROCK/MRTF-A and Erk1/2 signaling pathways.

    abstract::The mitral valve is a complex multilayered structure populated by fibroblast-like cells, valvular interstitial cells (VIC) which are embedded in an extracellular matrix (ECM) scaffold and are submitted to the mechanical deformations affecting valve at each heartbeat, for an average of 40 million times per year. Myxoma...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Blomme B,Deroanne C,Hulin A,Lambert C,Defraigne JO,Nusgens B,Radermecker M,Colige A

    更新日期:2019-10-01 00:00:00

  • Molecular mechanisms underlying the long-term impact of dietary fat to increase cardiac pyruvate dehydrogenase kinase: regulation by insulin, cyclic AMP and pyruvate.

    abstract::Previous studies have demonstrated that pyruvate dehydrogenase kinase (PDHK) activity in extracts of rat cardiac mitochondria is increased @two-fold by providing a high-fat diet for 28 days. The present study sought to establish the factor(s) that might underlie the response of cardiac PDHK to the provision of a high-...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Sugden MC,Orfali KA,Fryer LG,Holness MJ,Priestman DA

    更新日期:1997-07-01 00:00:00

  • Regulation of mTOR and S6K1 activation by the nPKC isoforms, PKCepsilon and PKCdelta, in adult cardiac muscle cells.

    abstract::Activation of both mTOR and its downstream target, S6K1 (p70 S6 kinase) have been implicated to affect cardiac hypertrophy. Our earlier work, in a feline model of 1-48 h pressure overload, demonstrated that mTOR/S6K1 activation occurred primarily through a PKC/c-Raf pathway. To further delineate the role of specific P...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Moschella PC,Rao VU,McDermott PJ,Kuppuswamy D

    更新日期:2007-12-01 00:00:00

  • Dok-5 is involved in cardiomyocyte differentiation through PKB/FOXO3a pathway.

    abstract::The insulin receptor substrate (IRS) family plays important roles in cellular growth, signaling, and survival in the brain. We identified IRS6/Dok-5, a member of the IRS family, also expressed in heart. Dok-5 expression level significantly increased during cardiomyocyte differentiation of P19CL6 cells. To understand t...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wen J,Xia Q,Wang C,Liu W,Chen Y,Gao J,Gong Y,Yin B,Ke Y,Qiang B,Yuan J,Peng X

    更新日期:2009-12-01 00:00:00

  • Autoantibodies from mothers of children with congenital heart block downregulate cardiac L-type Ca channels.

    abstract::Congenital heart block (CHB) affects offspring of mothers with autoantibodies (positive IgG) to intracellular SSA/Ro and SSB/La ribonucleoproteins and is associated with high morbidity and mortality. Here, we show that maternal anti-Ro/La antibodies immunoreact with human fetal cardiomyocyte sarcolemma, recognize huma...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Qu Y,Xiao GQ,Chen L,Boutjdir M

    更新日期:2001-06-01 00:00:00

  • Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210.

    abstract::It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Inoue T,Kobirumaki-Shimozawa F,Kagemoto T,Fujii T,Terui T,Kusakari Y,Hongo K,Morimoto S,Ohtsuki I,Hashimoto K,Fukuda N

    更新日期:2013-10-01 00:00:00

  • Reduced myocardial Na+, K(+)-pump capacity in congestive heart failure following myocardial infarction in rats.

    abstract::We examined changes in expression and function of the cardiac Na+, K(+)-pump in a post-infarction rat model of hypertrophy and congestive heart failure (CHF). Myocardial infarction was induced by ligation of the left coronary artery in Wistar rats and hearts were obtained from animals with CHF and from sham operated r...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Semb SO,Lunde PK,Holt E,Tønnessen T,Christensen G,Sejersted OM

    更新日期:1998-07-01 00:00:00

  • Ultrastructural changes induced in the isolated rat heart by enzymatically generated oxygen radicals.

    abstract::This study describes the effect of oxygen radicals on the ultrastructure of the isolated Langendorff-perfused rat heart. Oxygen radicals were enzymatically generated by xanthine oxidase (0.025 U/ml) and hypoxanthine (0.96 mM). Hearts were perfusion-fixed for electron microscopy and stereological technique was performe...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Ytrehus K,Myklebust R,Olsen R,Mjøs OD

    更新日期:1987-04-01 00:00:00

  • Overexpression of ANO1/TMEM16A, an arterial Ca2+-activated Cl- channel, contributes to spontaneous hypertension.

    abstract::Calcium-activated chloride channels (CaCCs) have been implicated in hypertension; however, the mechanism underlying their involvement is unknown. The aim of this study was to determine whether the CaCC ANO1 is involved in the pathogenesis of spontaneous hypertension. Arterial ANO1 expression and the effects on blood p...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wang B,Li C,Huai R,Qu Z

    更新日期:2015-05-01 00:00:00

  • Electrophysiologic and extracellular ionic changes during acute ischemia in failing and normal rabbit myocardium.

    abstract::The incidence of ventricular arrhythmias is higher in failing hearts than in control hearts, especially during acute ischemia. Electrophysiological and extracellular ionic changes during acute ischemia in normal and failing rabbit myocardium were assessed. Heart failure was induced in rabbits by combined volume and pr...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Vermeulen JT,Tan HL,Rademaker H,Schumacher CA,Loh P,Opthof T,Coronel R,Janse MJ

    更新日期:1996-01-01 00:00:00

  • Actin and myosin isoforms in aneural and malformed chick hearts.

    abstract::Although it is generally accepted that actin and myosin isoforms adapt to their functional requirements, the sequence of expression of these proteins in hearts developing abnormally is unknown. In the chick embryo it is possible to change various aspects of heart development without direct manipulation of the cardiova...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kirby ML,Shimizu N,Gagnon J,Toyofuku T,Kennedy J,Conrad DC,Zak R

    更新日期:1990-09-01 00:00:00

  • Differential protein expression profiling of myocardial tissue in a mouse model of hypertrophic cardiomyopathy.

    abstract::Hypertrophic cardiomyopathy (HCM) is a genetic disorder caused by mutations in genes encoding sarcomere proteins. The mechanisms involved in the development of cardiac hypertrophy and heart failure remain poorly understood. Global proteomic profiling was used to study the cardiac proteome of mice predisposed to develo...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Lam L,Tsoutsman T,Arthur J,Semsarian C

    更新日期:2010-05-01 00:00:00

  • Coronary collateral growth--back to the future.

    abstract::The coronary collateral circulation is critically important as an adaptation of the heart to prevent the damage from ischemic insults. In their native state, collaterals in the heart would be classified as part of the microcirculation, existing as arterial-arterial anastomotic connections in the range of 30 to 100 μM ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Chilian WM,Penn MS,Pung YF,Dong F,Mayorga M,Ohanyan V,Logan S,Yin L

    更新日期:2012-04-01 00:00:00

  • Comparative study on isolation and mitochondrial function of adult mouse and rat cardiomyocytes.

    abstract:BACKGROUND:Cultured adult mouse and rat cardiomyocytes are the best and low-cost cell model for cardiac cellular physiology, pathology, drug toxicity screening, and intervention. The functions of mouse cardiomyocytes decline faster than rat cardiomyocytes in culture conditions. However, little is known about the differ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Liu B,Li A,Qin Y,Tian X,Gao M,Jiang W,Gong G

    更新日期:2019-11-01 00:00:00

  • Effects of regional ischemia on the ryanodine-sensitive Ca2+ release channel of canine cardiac sarcoplasmic reticulum.

    abstract::In mammalian myocardium, muscle contraction is regulated by the rapid release of Ca2+ ions through ryanodine-sensitive Ca2+ release channels present in the intracellular membrane compartment, sarcoplasmic reticulum (SR). In this study, the effects of regional ischemia on intrinsic SR Ca2+ release channel function were...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Darling EM,Lai FA,Meissner G

    更新日期:1992-10-01 00:00:00

  • Regulation of L-type calcium channels of vascular smooth muscle cells.

    abstract::Vascular tone is regulated by a variety of neurotransmitters, vasoactive hormones and autacoids, and vasoactive drugs. These actions are mediated, at least in part, by actions on the membrane ion channels, exerted either directly or indirectly. In this article, we described evidence that four different protein kinase ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Xiong Z,Sperelakis N

    更新日期:1995-01-01 00:00:00

  • Sustained activation of ADP/P2ry12 signaling induces SMC senescence contributing to thoracic aortic aneurysm/dissection.

    abstract::Thoracic aortic aneurysm/dissection (TAAD) is characterized by excessive smooth muscle cell (SMC) loss, extracellular matrix (ECM) degradation and inflammation. However, the mechanism whereby signaling leads to SMC loss is unclear. We used senescence-associated (SA)-β-gal staining and analysis of expression of senesce...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Zhang WM,Liu Y,Li TT,Piao CM,Liu O,Liu JL,Qi YF,Jia LX,Du J

    更新日期:2016-10-01 00:00:00

  • Interaction of Gbetagamma with RACK1 and other WD40 repeat proteins.

    abstract::Heterotrimeric G-proteins, composed of Galpha and Gbetagamma subunits, transmit numerous and diverse extracellular stimuli via a large family of heptahelical cell-surface receptors to various intracellular effector molecules. The Gbetagamma subunit plays a central role in G-protein signaling. The Gbeta subunit belongs...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Chen S,Spiegelberg BD,Lin F,Dell EJ,Hamm HE

    更新日期:2004-08-01 00:00:00

  • Induction of antioxidant and detoxification response by oxidants in cardiomyocytes: evidence from gene expression profiling and activation of Nrf2 transcription factor.

    abstract::Mild or low doses of oxidants are known to prime cells towards resistance against further damage. In cardiomyocytes, we found that pretreatment with 100 microM H(2)O(2) prevents the cells from apoptosis induced by doxorubicin (Dox). Affymetrix microarray analyses of 28,000 genes reveal that H(2)O(2) treated cells redu...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Purdom-Dickinson SE,Lin Y,Dedek M,Morrissy S,Johnson J,Chen QM

    更新日期:2007-01-01 00:00:00

  • Reduced expression of mitochondrial electron transport chain proteins from hibernating hearts relative to ischemic preconditioned hearts in the second window of protection.

    abstract::Although protection against necrosis has been observed in both hibernating (HIB) and ischemic preconditioned hearts in the second window of protection (SWOP), a comparison of the mitochondrial proteome between the two entities has not been previously performed. Anesthetized swine underwent instrumentation with a fixed...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Cabrera JA,Butterick TA,Long EK,Ziemba EA,Anderson LB,Duffy CM,Sluiter W,Duncker DJ,Zhang J,Chen Y,Ward HB,Kelly RF,McFalls EO

    更新日期:2013-07-01 00:00:00