Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.


:We screened 29 human esophageal squamous cell carcinoma (ESC) cell lines for mutations of the p53 gene through all coding exons and exon-intron junctions. Mutations were found in 22 cell lines (76%), consisting of 20 single-base substitutions, 2 small deletions and 1 single-base insertion. Out of 20 single-base substitution, 5 were located at the exon-intron junctions and mRNAs with abnormal splicing were detected by RT-PCR in 4 of them. A G:C to T:A transversion, which occurred rather frequently in resected tumors of ESC, was observed in only 1 cell line, and, instead, frequent transitions at CpG sites were detected. We also examined 65 fresh tumor materials, from all of which we tried to establish cell lines, and detected mutations in 26 samples (40%). Compared with the results in these fresh tumor materials, the mutation incidence in cell lines was significantly high and the mutation spectrum was also different. From these 65 tumors, 10 cell lines were established, including 3 cell lines from 26 tumors with p53 mutations and 7 cell lines from 39 without mutations, which indicates that there was no significant correlation between the status of the p53 gene in each fresh tumor and its establishment as a cell line. In 7 cell lines established from mutation-free tumors, newly acquired mutations were detected in 5, which suggests that mutations might occur during the process of establishing cell lines.


Int J Cancer


Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K




Has Abstract


1996-01-26 00:00:00














  • Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells.

    abstract::Lysine-specific demethylase 1 (LSD1), a histone lysine demethylase with the main specificity for H3K4me2, has been shown to be overexpressed in rhabdomyosarcoma (RMS) tumor samples. However, its role in RMS biology is not yet well understood. Here, we identified a new role of LSD1 in regulating adhesion of RMS cells. ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Haydn T,Kehr S,Willmann D,Metzger E,Schüle R,Fulda S

    更新日期:2020-06-15 00:00:00

  • Cytogenetic abnormalities in benign lymphoid hyperplasia: a dual-parameter study using chromosome analysis and flow cytometry.

    abstract::This is a prospective study of lymphoid tissue showing benign reactive hyperplasia (18 lymph nodes and 2 tonsils), using cytogenetic analysis of cells stimulated with T- or B-cell mitogens. The reason for this study was the detection of an abnormal chromosomal population in cells from an enlarged lymph node excised fr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Grace J,Hall BE,Lew M,Singh S,Pittman S,Palmer AA,Zbroja R,Wass J,Vincent PC

    更新日期:1989-12-15 00:00:00

  • Uptake of prostate-specific antigen testing for early prostate cancer detection in Sweden.

    abstract::The aim of our study was to estimate uptake of prostate-specific antigen (PSA) testing in an entire country, including time trends and geographical differences. Data from the Swedish Cancer Register on prostate cancer incidence between 1980 and 2007 and published data from the Gothenburg branch of the European randomi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jonsson H,Holmström B,Duffy SW,Stattin P

    更新日期:2011-10-15 00:00:00

  • Effect of tunicamycin on synthesis and secretion of carcinoembryonic antigen by human colonic adenocarcinoma cells.

    abstract::The effect of the glycosylation inhibitor, tunicamycin, on synthesis and secretion of the membrane-associated glycoprotein carcinoembryonic antigen (CEA), was studied in the LS174T human colon cancer cell line. Tunicamycin treatment inhibited total cellular glycoprotein synthesis but did not affect CEA levels of cellu...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Miura S,Kim YS

    更新日期:1986-08-15 00:00:00

  • The 5'-flanking region of human CD24 gene has cell-type-specific promoter activity in small-cell lung cancer.

    abstract::The human surface antigen CD24 is over-expressed in small-cell lung cancer. Here we describe the isolation, sequencing and functional characterization of the 5'-flanking region of the human CD24 gene. A sequence (accession number: Y14692) of 3.4 kb regulates the activity of a luciferase reporter gene in CD24-expressin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pass MK,Quintini G,Zarn JA,Zimmermann SM,Sigrist JA,Stahel RA

    更新日期:1998-11-09 00:00:00

  • Incidence of childhood cancer in Cuba (1986-1990).

    abstract::Cancer is an infrequent disease in childhood. However, it is responsible for 13.06% of child deaths between 1 and 14 years in Cuba. The aim of our work was to describe the incidence of childhood cancer in the period 1986 to 1990 using data reported to the National Cancer Registry (NCR) of Cuba. All cancer cases betwee...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Martín AA,Alert JA,Renó JS,Lonchong M,Grueiro S

    更新日期:1997-08-07 00:00:00

  • Elevated hTERT mRNA levels: A potential determinant of bronchial squamous cell carcinoma (in situ).

    abstract::Expression levels of hTERT mRNA were investigated by RT-PCR in tissue specimens of patients with (Group A) and without (Group B) clinically overt bronchial carcinoma, respectively. Bronchial carcinoma (n = 9) and distant normal (n = 9) specimens were analyzed in Group A. The chance of carcinoma seemed to increase with...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Snijders PJ,Breuer RH,Sutedja TG,Egging M,Voorhorst FJ,Steenbergen RD,van der Linden HC,Risse EK,Berkhof J,de Vries EG,van der Zee AG,Postmus PE,Meijer CJ,Smit EF

    更新日期:2004-04-10 00:00:00

  • Postsurgical chemotherapy in stage IB nonsmall cell lung cancer: Long-term survival in a randomized study.

    abstract::Although surgical resection is considered the adequate treatment in early stages of nonsmall cell lung cancer, long-term survival is not satisfactory and recurrence rate is high. We previously showed that postoperative chemotherapy at stage IB reduces recurrences and prolongs overall survival. We extended size and obs...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Roselli M,Mariotti S,Ferroni P,Laudisi A,Mineo D,Pompeo E,Ambrogi V,Mineo TC

    更新日期:2006-08-15 00:00:00

  • Targeting FAK scaffold functions inhibits human renal cell carcinoma growth.

    abstract::Human conventional renal cell carcinoma (CCC) remains resistant to current therapies. Focal Adhesion Kinase (FAK) is upregulated in many epithelial tumors and clearly implicated in nearly all facets of cancer. However, only few reports have assessed whether FAK may be associated with renal tumorigenesis. In this study...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Béraud C,Dormoy V,Danilin S,Lindner V,Béthry A,Hochane M,Coquard C,Barthelmebs M,Jacqmin D,Lang H,Massfelder T

    更新日期:2015-10-01 00:00:00

  • Estimating the impact of an organised screening programme on cervical cancer incidence: A 26-year study from northern Italy.

    abstract::The impact of the organised cervical cancer (CC) screening programmes implemented in Europe since the 1990s has been insufficiently evaluated. We investigated the changes in CC incidence following the introduction of a screening programme in the Emilia-Romagna Region (northern Italy). The study period was 1988-2013. T...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bucchi L,Baldacchini F,Mancini S,Ravaioli A,Giuliani O,Vattiato R,Falcini F,Giorgi Rossi P,Campari C,Canuti D,Di Felice E,Sassoli de Bianchi P,Ferretti S,Bertozzi N,Biggeri A,Emilia-Romagna Region Workgroup for Cervical Scr

    更新日期:2019-03-01 00:00:00

  • GD2-mediated melanoma cell targeting and cytotoxicity of liposome-entrapped fenretinide.

    abstract::Melanoma is a highly malignant and increasingly common neoplasm. Because metastatic melanoma remains incurable, new treatment approaches are needed. Immunoliposomes have been previously shown to enhance the selective localization of immunoliposome-entrapped drugs to solid tumors with improvements in the therapeutic in...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pagnan G,Montaldo PG,Pastorino F,Raffaghello L,Kirchmeier M,Allen TM,Ponzoni M

    更新日期:1999-04-12 00:00:00

  • Human ZNF312b oncogene is regulated by Sp1 binding to its promoter region through DNA demethylation and histone acetylation in gastric cancer.

    abstract::In a previous study, human ZNF312b was identified as a cell proliferation-associated oncogene via the K-ras/extracellular signal-regulated kinase cascade in gastric cancer. However, the mechanism concerning its transcriptional activation remains unknown. Here, we show that DNA methylation and histone acetylation of th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Song IS,Ha GH,Kim JM,Jeong SY,Lee HC,Kim YS,Kim YJ,Kwon TK,Kim NS

    更新日期:2011-11-01 00:00:00

  • Identification of a subset of pericytes that respond to combination therapy targeting PDGF and VEGF signaling.

    abstract::The aim of our study was to further explore the use of anti-angiogenic therapy targeting the vascular endothelial growth factor receptor (VEGFR) on endothelial cells while simultaneously targeting platelet-derived growth factor receptors (PDGFRs) on adjacent pericytes. B16 mouse melanoma tumors exogenously expressing ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hasumi Y,Kłosowska-Wardega A,Furuhashi M,Ostman A,Heldin CH,Hellberg C

    更新日期:2007-12-15 00:00:00

  • Immortalization of oral keratinocytes by functional inactivation of the p53 and pRb pathways.

    abstract::A subgroup of head and neck squamous cell carcinomas (HNSCCs) contains high-risk human papillomavirus-type 16 (HPV16). The viral E6 and E7 oncoproteins inactivate the p53 and pRb proteins, respectively. We examined the causative effect of HPV16 E6 and E7 expression on the immortalization of normal oral keratinocytes (...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Smeets SJ,van der Plas M,Schaaij-Visser TB,van Veen EA,van Meerloo J,Braakhuis BJ,Steenbergen RD,Brakenhoff RH

    更新日期:2011-04-01 00:00:00

  • The C-terminus of apoptin represents a unique tumor cell-enhanced nuclear targeting module.

    abstract::Chicken anemia virus viral protein 3 (VP3 or apoptin) localizes more efficiently in the nucleus of transformed than nontransformed cells. Although previous studies implicate the C-terminus of apoptin as being responsible, the molecular basis is controversial, and the extent to which altered nuclear transport efficienc...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kuusisto HV,Wagstaff KM,Alvisi G,Jans DA

    更新日期:2008-12-15 00:00:00

  • Fowlpox-based survivin vaccination for malignant mesothelioma therapy.

    abstract::Survivin protein is an attractive candidate for cancer immunotherapy since it is abundantly expressed in most common human cancers and mostly absent in normal adult tissues. Malignant mesothelioma (MM) is a deadly cancer associated with asbestos or erionite exposure for which no successful therapies are currently avai...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bertino P,Panigada M,Soprana E,Bianchi V,Bertilaccio S,Sanvito F,Rose AH,Yang H,Gaudino G,Hoffmann PR,Siccardi A,Carbone M

    更新日期:2013-08-01 00:00:00

  • Differential effects of retinoic acid on the in vitro growth and cell-surface glycoconjugates of 2 human head and neck squamous-cell carcinomas.

    abstract::As a part of an assessment of the potential use of retinoids in preventive and adjuvant treatment of HNSCC, we examined the effects of beta-all-trans retinoic acid (RA) on the growth and cell-surface glycoconjugates of 2 HNSCC cell lines. These lines, designated 1483 and 183A, were established from an untreated patien...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lotan R,Sacks PG,Lotan D,Hong WK

    更新日期:1987-08-15 00:00:00

  • RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells.

    abstract::A high molecular weight RNA-reverse transcriptase complex in the culture media of peripheral leukocytes obtained from two Japanese patients with myeloma-leukemia was detected by demonstration of a 3H-uridine peak and a peak of DNA polymerizing activity banding at a density of 1.15-1.19g/ml. The enzyme in the complex w...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sawada H,Tashima M,Nakamura T,Uchiyama T,Sagawa K,Takatsuki K,Uchino H,Ito Y

    更新日期:1977-07-15 00:00:00

  • Serum concentrations of squamous cell carcinoma antigen in patients with vulvar intraepithelial neoplasia and vulvar cancer.

    abstract::Our aim was to evaluate whether serum concentrations of squamous cell carcinoma antigen (SCC-Ag) are an independent prognostic factor in patients with vulvar cancer. We measured SCC-Ag in pretreatment serum samples of 55 patients with squamous cell vulvar cancer, 30 patients with vulvar intraepithelial neoplasia (VIN)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hefler L,Obermair A,Tempfer C,van Houte M,Maenner G,Reinthaller A,Leodolter S,Kainz C

    更新日期:1999-06-21 00:00:00

  • Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.

    abstract::The insulin like growth factor (IGF) signaling pathway has been shown to contribute to melanoma progression, but little is known about the role of the IGF binding protein 3 (IGFBP-3) in melanoma biology. The aim of the present study was to characterize expression, function and regulation of IGFBP-3 in malignant melano...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Oy GF,Slipicevic A,Davidson B,Solberg Faye R,Maelandsmo GM,Flørenes VA

    更新日期:2010-01-15 00:00:00

  • Effects of the exercise-inducible myokine irisin on malignant and non-malignant breast epithelial cell behavior in vitro.

    abstract::Exercise has been shown to reduce risk and improve prognosis of several types of cancers. Irisin is a myokine linked to exercise and lean body mass, which is thought to favorably alter metabolism systemically, potentially providing benefit for metabolic disease (including cancer). We evaluated the effects of various c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gannon NP,Vaughan RA,Garcia-Smith R,Bisoffi M,Trujillo KA

    更新日期:2015-02-15 00:00:00

  • Gallstones and incident colorectal cancer in a large pan-European cohort study.

    abstract::Gallstones, a common gastrointestinal condition, can lead to several digestive complications and can result in inflammation. Risk factors for gallstones include obesity, diabetes, smoking and physical inactivity, all of which are known risk factors for colorectal cancer (CRC), as is inflammation. However, it is unclea...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ward HA,Murphy N,Weiderpass E,Leitzmann MF,Aglago E,Gunter MJ,Freisling H,Jenab M,Boutron-Ruault MC,Severi G,Carbonnel F,Kühn T,Kaaks R,Boeing H,Tjønneland A,Olsen A,Overvad K,Merino S,Zamora-Ros R,Rodríguez-Barranc

    更新日期:2019-09-15 00:00:00

  • Characterization of a highly invasive and spontaneously metastatic human malignant melanoma cell line.

    abstract::Although the incidence of, and deaths due to, malignant melanoma are rising at a rapid rate, few experimental models mimic the highly metastatic properties associated with the pathogenesis of the human disease, making study of the disease difficult. Thus, new human models are required to understand melanoma biology, e...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Welch DR,Bisi JE,Miller BE,Conaway D,Seftor EA,Yohem KH,Gilmore LB,Seftor RE,Nakajima M,Hendrix MJ

    更新日期:1991-01-21 00:00:00

  • A case-control study of diet and lung cancer in northeast China.

    abstract::A case-control study involving interviews with 227 lung-cancer cases and 227 matched hospital controls was conducted in Heilongjiang Province in northeast China to examine the influence of dietary factors on the risk of developing lung cancer. Lung-cancer cases were all incident cases judged to be suitable candidates ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hu J,Johnson KC,Mao Y,Xu T,Lin Q,Wang C,Zhao F,Wang G,Chen Y,Yang Y

    更新日期:1997-06-11 00:00:00

  • Expression of human telomerase subunits in ovarian malignant, borderline and benign tumors.

    abstract::Telomerase activity is involved in the maintenance of telomere length and is thought to be required for cellular immortality and oncogenesis. Three major subunits composing telomerase, human telomerase RNA (hTR), telomerase-associated protein (TPI) and human telomerase catalytic subunit (hTERT), have been identified. ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kyo S,Kanaya T,Takakura M,Tanaka M,Yamashita A,Inoue H,Inoue M

    更新日期:1999-03-15 00:00:00

  • Variant estrogen receptor alpha mRNAs in human breast cancer specimens.

    abstract::A panel of human breast cancer specimens was examined for single base change mutations by DNA sequencing and for larger deletions using a PCR-based assay. In the cancer specimens examined, no sequencing variants were detected other than a previously characterized polymorphism. Although most of the specimens contained ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Anandappa SY,Sibson R,Platt-Higgins A,Winstanley JH,Rudland PS,Barraclough R

    更新日期:2000-10-15 00:00:00

  • Paracrine regulation of glioma cells invasion by astrocytes is mediated by glial-derived neurotrophic factor.

    abstract::It was suggested that the brain microenvironment plays a role in glioma progression. Here we investigate the mechanism by which astrocytes which are abundant in glioma tumors, promote cancer cell invasion. In this study, we evaluated the effects of astrocytes on glioma biology both in vitro and in vivo and determined ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Shabtay-Orbach A,Amit M,Binenbaum Y,Na'ara S,Gil Z

    更新日期:2015-09-01 00:00:00

  • Differential expression of sphingolipids in MRP1 overexpressing HT29 cells.

    abstract::We have obtained a novel multidrug resistant cell line, derived from HT29 G(+) human colon carcinoma cells, by selection with gradually increasing concentrations of the anti-mitotic, microtubule-disrupting agent colchicine. This HT29(col) cell line displayed a 25-fold increase in colchicine resistance and exhibited cr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kok JW,Veldman RJ,Klappe K,Koning H,Filipeanu CM,Müller M

    更新日期:2000-07-15 00:00:00

  • Glypican-3, overexpressed in hepatocellular carcinoma, modulates FGF2 and BMP-7 signaling.

    abstract::The Glypican (GPC) family is a prototypical member of the cell-surface heparan sulfate proteoglycans (HSPGs). The HSPGs have been demonstrated to interact with growth factors, act as coreceptors and modulate growth factor activity. Here we show that based on oligonucleotide array analysis, GPC3 was upregulated in hepa...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Midorikawa Y,Ishikawa S,Iwanari H,Imamura T,Sakamoto H,Miyazono K,Kodama T,Makuuchi M,Aburatani H

    更新日期:2003-02-10 00:00:00

  • Cigarette smoking, alcohol consumption and primary liver cancer: a case-control study in the USA.

    abstract::Cigarette smoking and alcohol consumption were examined as potential risk factors in a case-control study of primary liver cancer (PLC). A total of 165 PLC cases and 465 matched controls from several US hospitals were studied. A weak but statistically significant (p less than 0.05) dose-response relationship was obser...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yu H,Harris RE,Kabat GC,Wynder EL

    更新日期:1988-09-15 00:00:00