Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.


:We screened 29 human esophageal squamous cell carcinoma (ESC) cell lines for mutations of the p53 gene through all coding exons and exon-intron junctions. Mutations were found in 22 cell lines (76%), consisting of 20 single-base substitutions, 2 small deletions and 1 single-base insertion. Out of 20 single-base substitution, 5 were located at the exon-intron junctions and mRNAs with abnormal splicing were detected by RT-PCR in 4 of them. A G:C to T:A transversion, which occurred rather frequently in resected tumors of ESC, was observed in only 1 cell line, and, instead, frequent transitions at CpG sites were detected. We also examined 65 fresh tumor materials, from all of which we tried to establish cell lines, and detected mutations in 26 samples (40%). Compared with the results in these fresh tumor materials, the mutation incidence in cell lines was significantly high and the mutation spectrum was also different. From these 65 tumors, 10 cell lines were established, including 3 cell lines from 26 tumors with p53 mutations and 7 cell lines from 39 without mutations, which indicates that there was no significant correlation between the status of the p53 gene in each fresh tumor and its establishment as a cell line. In 7 cell lines established from mutation-free tumors, newly acquired mutations were detected in 5, which suggests that mutations might occur during the process of establishing cell lines.


Int J Cancer


Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K




Has Abstract


1996-01-26 00:00:00














  • Curcumin induces cell-arrest and apoptosis in association with the inhibition of constitutively active NF-kappaB and STAT3 pathways in Hodgkin's lymphoma cells.

    abstract::Although treatment of Hodgkin's lymphoma (HL) with a multi-drug approach has been very successful, its toxicity becomes evident after several years as secondary malignancies and cardiovascular disease. Therefore, the current goal in HL treatment is to find new therapies that specifically target the deregulated signali...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Mackenzie GG,Queisser N,Wolfson ML,Fraga CG,Adamo AM,Oteiza PI

    更新日期:2008-07-01 00:00:00

  • Cell proliferation in 3,800 node-negative breast cancers: consistency over time of biological and clinical information provided by 3H-thymidine labelling index.

    abstract::For breast cancer, many prognostic markers that initially appeared promising have failed to maintain their clinical predictive value. Few reports have analyzed the consistency over time of biological and clinical information provided by biomarkers. Tumour cell proliferation has acquired relevance as an indicator of pr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Silvestrini R,Daidone MG,Luisi A,Mastore M,Leutner M,Salvadori B

    更新日期:1997-02-20 00:00:00

  • Expression of the tyrosine kinase c-kit is an independent prognostic factor in patients with small cell lung cancer.

    abstract::In a retrospective analysis of 203 patients with small cell lung cancer (SCLC), we examined the prognostic value of c-kit expression on survival. Expression of c-kit was examined immunohistochemically in formalin-fixed, paraffin-embedded tissue sections. c-kit was observed in 87.7% of SCLC tumors. Using the Kaplan-Mei...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rohr UP,Rehfeld N,Pflugfelder L,Geddert H,Müller W,Steidl U,Fenk R,Gräf T,Schott M,Thiele KP,Gabbert HE,Germing U,Kronenwett R,Haas R

    更新日期:2004-08-20 00:00:00

  • Circulating levels of sex steroid hormones and risk of endometrial cancer in postmenopausal women.

    abstract::Experimental and epidemiological data support a role for sex steroid hormones in the pathogenesis of endometrial cancer. The associations of pre-diagnostic blood concentrations of estradiol, estrone, testosterone, androstenedione, DHEAS and SHBG with endometrial cancer risk were investigated. A case-control study was ...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Lukanova A,Lundin E,Micheli A,Arslan A,Ferrari P,Rinaldi S,Krogh V,Lenner P,Shore RE,Biessy C,Muti P,Riboli E,Koenig KL,Levitz M,Stattin P,Berrino F,Hallmans G,Kaaks R,Toniolo P,Zeleniuch-Jacquotte A

    更新日期:2004-01-20 00:00:00

  • Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model.

    abstract::The EWS/Fli-1 fusion gene, a product of the translocation t(11;22, q24;q12), is detected in 85% of Ewing sarcomas and primitive neuroectodermal tumors. It is thought to be a transcriptional activator that plays a significant role in tumorigenesis. In this study, we developed a novel EWS/Fli-1 blockade system using RNA...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Takigami I,Ohno T,Kitade Y,Hara A,Nagano A,Kawai G,Saitou M,Matsuhashi A,Yamada K,Shimizu K

    更新日期:2011-01-01 00:00:00

  • p16/MTS1 inactivation in ovarian carcinomas: high frequency of reduced protein expression associated with hyper-methylation or mutation in endometrioid and mucinous tumors.

    abstract::Inactivation of the tumor-suppressor gene p16 (MTS1/ CDKN2/INK4a) has been described in various human malignancies. Although p16 deletion has been found in various ovarian tumor cell lines, p16 inactivation by homozygous deletion or mutation has been reported only sporadically in primary ovarian carcinomas. In a compr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Milde-Langosch K,Ocon E,Becker G,Löning T

    更新日期:1998-02-20 00:00:00

  • Isoenzyme pattern and subcellular localization of hexokinases in human breast cancer and nonpathological breast tissue.

    abstract::Subcellular distribution of hexokinase (HK) isoenzymes in 22 human breast cancers (21 primary cancers and 1 axillary metastatic growth) and 7 non-pathological human mammary gland tissue samples was studied with starch gel electrophoresis on isolated cell fractions obtained by differential centrifugation. Fractions use...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gudnason V,Ingvarsson S,Jonasdottir A,Andresdottir V,Egilsson V

    更新日期:1984-07-15 00:00:00

  • HLA-B alleles, high-risk HPV infection and risk for cervical neoplasia in southern Chinese women.

    abstract::A population-based study was conducted on 256 southern Chinese with cervical intraepithelial neoplasia grade III (CIN III) or invasive cervical cancer (ICC) and on 258 controls to examine the associations between HLA-B alleles, infection with high-risk human papillomaviruses (HPVs) and the development of cervical neop...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chan PK,Cheung JL,Cheung TH,Lin CK,Tam AO,Chan DP,Zhou DX,Lo KW,Yim SF,Siu SS

    更新日期:2006-03-15 00:00:00

  • Functional impact of sequence variation in the promoter region of TGFB1.

    abstract::Pathological deregulation of the transforming growth factor, beta 1 (TGFB1) pathway has been implicated in the development of several major diseases, including cancers. Regulatory variation in the TGFB1 gene may lead to altered TGFB1 expression and activity, and thus, modulate an individual's susceptibility to disease...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Healy J,Dionne J,Bélanger H,Larivière M,Beaulieu P,Labuda D,Sinnett D

    更新日期:2009-09-15 00:00:00

  • The importance of 5-HT for tumor cell lodgement in the liver.

    abstract::Arrest and lodgement of circulating tumor cells in the microvasculature is a prerequisite for metastasis formation. Aggregation of activated platelets and subsequent release of bioactive substances seem to influence the lodgement process. Since thrombocytopenia has been shown to reduce lodgement and since 5-HT is one ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Skolnik G,Bagge U,Dahlström A,Ahlman H

    更新日期:1984-04-15 00:00:00

  • HS3ST2 modulates breast cancer cell invasiveness via MAP kinase- and Tcf4 (Tcf7l2)-dependent regulation of protease and cadherin expression.

    abstract::Heparan sulfate 3-O-sulfotransferase 2 (HS3ST2), an enzyme mediating 3-O-sulfation of heparan sulfate (HS), is silenced by hypermethylation in breast cancer. As HS has an important co-receptor function for numerous signal transduction pathways, the phenotypical changes due to HS3ST2 reexpression were investigated in v...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Vijaya Kumar A,Salem Gassar E,Spillmann D,Stock C,Sen YP,Zhang T,Van Kuppevelt TH,Hülsewig C,Koszlowski EO,Pavao MS,Ibrahim SA,Poeter M,Rescher U,Kiesel L,Koduru S,Yip GW,Götte M

    更新日期:2014-12-01 00:00:00

  • Better outcomes of modified myeloablative conditioning without antithymocyte globulin versus myeloablative conditioning in cord blood transplantation for hematological malignancies: A retrospective (development) and a prospective (validation) study.

    abstract::Cord blood transplantation (CBT) is an effective option for treating hematological malignancies, but graft failure (GF) remains the primary cause of therapy failure. Thus, based on myeloablative conditioning (MAC) of busulfan with cyclophosphamide (Bu/Cy) or total body irradiation with Cy (TBI/Cy), fludarabine (Flu) w...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sun Z,Liu H,Luo C,Geng L,Zheng C,Tang B,Zhu X,Tong J,Wang X,Ding K,Wan X,Zhang L,Yao W,Song K,Zhang X,Wu Y,Yang H,Han Y,Liu X,Zhu W,Wu J,Wang Z

    更新日期:2018-08-01 00:00:00

  • Murine osteosarcoma primary tumour growth and metastatic progression is maintained after marked suppression of serum insulin-like growth factor I.

    abstract::The insulin-like growth factor I (IGF-I) signaling pathway has been shown to play an important role in several aspects of cancer biology, including metastasis. The aim of this study was to define the contribution of serum (endocrine) and local (tumour microenvironment) IGF-I on osteosarcoma tumour growth and metastasi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hong SH,Briggs J,Newman R,Hoffman K,Mendoza A,LeRoith D,Helman L,Yakar S,Khanna C

    更新日期:2009-05-01 00:00:00

  • The presence of concanavalin-A(Con-A)-like molecules on natural-killer (NK)-sensitive target cells: their possible role in swainsonine-augmented human NK cytotoxicity.

    abstract::In the present study we examined the expression of concanavalin-A(Con-A)-like molecules on natural-killer (NK)-sensitive target cells and investigated their possible role in the human NK-cell phenomenon. The incubation of either peripheral-blood lymphocytes (PBL) or large granular lymphocytes (LGL) with swainsonine (S...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yagita M,Noda I,Maehara M,Fujieda S,Inoue Y,Hoshino T,Saksela E

    更新日期:1992-10-21 00:00:00

  • Breast cancer survival in sub-Saharan Africa by age, stage at diagnosis and human development index: A population-based registry study.

    abstract::Breast cancer is the leading cancer diagnosis and second most common cause of cancer deaths in sub-Saharan Africa (SSA). Yet, there are few population-level survival data from Africa and none on the survival differences by stage at diagnosis. Here, we estimate breast cancer survival within SSA by area, stage and count...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Joko-Fru WY,Miranda-Filho A,Soerjomataram I,Egue M,Akele-Akpo MT,N'da G,Assefa M,Buziba N,Korir A,Kamate B,Traore C,Manraj S,Lorenzoni C,Carrilho C,Hansen R,Finesse A,Somdyala N,Wabinga H,Chingonzoh T,Borok M,Chok

    更新日期:2020-03-01 00:00:00

  • The association between markers of tumour cell metabolism, the tumour microenvironment and outcomes in patients with colorectal cancer.

    abstract::Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Roseweir AK,Clark J,McSorley ST,vanWyk HC,Quinn JA,Horgan PG,McMillan DC,Park JH,Edwards J

    更新日期:2019-05-01 00:00:00

  • ALDH1A1 in patient-derived bladder cancer spheroids activates retinoic acid signaling leading to TUBB3 overexpression and tumor progression.

    abstract::Acquired chemoresistance is a critical issue for advanced bladder cancer patients during long-term treatment. Recent studies reveal that a fraction of tumor cells with enhanced tumor-initiating potential, or cancer stem-like cells (CSCs), may particularly contribute to acquired chemoresistance and recurrence. Thus, CS...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Namekawa T,Ikeda K,Horie-Inoue K,Suzuki T,Okamoto K,Ichikawa T,Yano A,Kawakami S,Inoue S

    更新日期:2020-02-15 00:00:00

  • Analgesics and cancers of the renal pelvis and ureter.

    abstract::To evaluate renal pelvis and ureter (RPU) cancer risk in relation to lifetime use of analgesics, a population-based case-control study was carried out in 3 areas of the United States. Among 502 cases and 496 controls diagnosed and interviewed during 1983-1986, no significant increases in risk were found for any of the...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Linet MS,Chow WH,McLaughlin JK,Wacholder S,Yu MC,Schoenberg JB,Lynch C,Fraumeni JF Jr

    更新日期:1995-07-04 00:00:00

  • Enzymatic oxidation products of spermine induce greater cytotoxic effects on human multidrug-resistant colon carcinoma cells (LoVo) than on their wild-type counterparts.

    abstract::The occurrence of resistance to cytotoxic agents in tumor cells, associated with several phenotypic alterations, is one of the major obstacles to successful anticancer chemotherapy. A new strategy to overcome MDR of human cancer cells was studied, using BSAO, which generates cytotoxic products from spermine, H(2)O(2) ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Calcabrini A,Arancia G,Marra M,Crateri P,Befani O,Martone A,Agostinelli E

    更新日期:2002-05-01 00:00:00

  • Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. Effects of small light doses on localization, dye fluorescence and photosensitivity in V79 cells.

    abstract::V79 cells incubated with di- or tetrasulfonated aluminium phthalocyanines (AlPcS2 or AlPcS4) showed a granular fluorescence pattern. Co-staining with the lysosomotropic dye acridine orange (AO) indicated that the granules that were stained by these photoactive phthalocyanines were identical to lysosomes. Small light e...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moan J,Berg K,Anholt H,Madslien K

    更新日期:1994-09-15 00:00:00

  • Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR.

    abstract::Cancer-germline genes (CGGs) code for immunogenic antigens that are present on various human tumors but not on normal tissues. The importance of CGGs in cancer immunotherapy has led to detailed studies of their expression in a range of human tumors. We measured the levels of expression of 12 CGGs in various pediatric ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jacobs JF,Brasseur F,Hulsbergen-van de Kaa CA,van de Rakt MW,Figdor CG,Adema GJ,Hoogerbrugge PM,Coulie PG,de Vries IJ

    更新日期:2007-01-01 00:00:00

  • Genetic analysis of in vitro leukemogenesis induced by thymus epithelial reticulum cells transmitting murine leukemia viruses.

    abstract::Lymphatic leukemia developed in C57BL/6 mice following inoculation of normal thymocytes that and been co-cultured on leukemic thymus epithelial reticulum monolayer cells. Using thymocytes genetically marked in Ly membrane antigens, we showed that the thymomas which developed were produced by the co-cultured thymocytes...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Haas M

    更新日期:1978-01-15 00:00:00

  • Binding activities of cis-platin-damage-recognition proteins in human tumour cell lines.

    abstract::Proteins can be detected by South-western analyses of human tumour-cell extracts binding to double-stranded oligonucleotide DNA treated in vitro with the chemotherapeutic drug cis-diamminedichloroplatinum (II) (CDDP), but not to untreated DNA. The relative molecular masses of proteins binding to the CDDP-treated doubl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: McLaughlin K,Coren G,Masters J,Brown R

    更新日期:1993-02-20 00:00:00

  • Survival in patients with human papillomavirus positive tonsillar cancer in relation to treatment.

    abstract::The incidence of tonsillar cancer and the proportion of human papillomavirus (HPV) positive tonsillar cancer cases have increased in the last decades. In parallel, treatment for tonsillar cancer has been intensified e.g., by accelerated radiotherapy, and chemotherapy, resulting in more side effects. Patients with HPV-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Attner P,Näsman A,Du J,Hammarstedt L,Ramqvist T,Lindholm J,Munck-Wikland E,Dalianis T,Marklund L

    更新日期:2012-09-01 00:00:00

  • Loss of specific natural cell-mediated cytotoxicity with absorption of natural antibodies from serum.

    abstract::When effector cells from most individuals are tested directly against a number of target cells for cell-mediated cytotoxicity, most of the reactivity is non-selective. This apparent non-selectiveness is a result of specific activity against multiple antigens on target cells by each effector suspension. The specific re...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Akira D,Takasugi M

    更新日期:1977-06-15 00:00:00

  • Association of DNA base excision repair genes (OGG1, APE1 and XRCC1) polymorphisms with outcome to platinum-based chemotherapy in advanced nonsmall-cell lung cancer patients.

    abstract::Polymorphism of DNA base excision repair (BER) genes affects DNA repair capacity and may alter sensitivity to platinum-based chemotherapy regimens. This study investigated polymorphisms of OGG1 Ser326Cys, APE1 Asp148Glu APE1-141T/G and XRCC1 Arg399Gln for association with clinical outcome in 235 advanced inoperable no...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Peng Y,Li Z,Zhang S,Xiong Y,Cun Y,Qian C,Li M,Ren T,Xia L,Cheng Y,Wang D

    更新日期:2014-12-01 00:00:00

  • Active oxygen transforms murine myeloid progenitor cells in vitro.

    abstract::Active oxygen (AO) is ubiquitous in nature and its many forms can act as natural carcinogens. Their effect on the transformation of a mouse myeloid progenitor cell line was studied using anchorage-independent colony formation in methylcellulose as the primary assay. Both cytotoxic and non-toxic concentrations of t-but...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Crawford DR,Greenberger JS

    更新日期:1991-11-11 00:00:00

  • Cerebrovascular inflammation promotes the formation of brain metastases.

    abstract::Brain metastases are the most prevalent intracranial malignancy. Patient outcome is poor and treatment options are limited. Hence, new avenues must be explored to identify potential therapeutic targets. Inflammation is a known critical component of cancer progression. Intratumoral inflammation drives progression and l...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sikpa D,Whittingstall L,Fouquet JP,Radulska A,Tremblay L,Lebel R,Paquette B,Lepage M

    更新日期:2020-07-01 00:00:00

  • Several genes encoding ribosomal proteins are over-expressed in prostate-cancer cell lines: confirmation of L7a and L37 over-expression in prostate-cancer tissue samples.

    abstract::A cDNA library specific for mRNA over-expressed in prostate cancer was generated by subtractive hybridization of transcripts originating from prostatic hyperplasia and cancer tissues. cDNA encoding ribosomal proteins L4, L5, L7a, L23a, L30, L37, S14 and S18 was found to be present among 100 analyzed clones. Levels of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Vaarala MH,Porvari KS,Kyllönen AP,Mustonen MV,Lukkarinen O,Vihko PT

    更新日期:1998-09-25 00:00:00

  • Chromosome 18 deletions are common events in classical midgut carcinoid tumors.

    abstract::Classical midgut carcinoids are rare intestinal neuroendocrine tumors that often present with metastases at diagnosis. In contrast to foregut carcinoids, midgut carcinoids are not related to the multiple endocrine neoplasia type 1 syndrome, and the mechanisms involved in their tumorigenesis are unknown. Eight classica...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Löllgen RM,Hessman O,Szabo E,Westin G,Akerström G

    更新日期:2001-06-15 00:00:00