Controlled release of platelet-derived growth factor using ethylene vinyl acetate copolymer (EVAc) coated on stainless-steel wires.


:Controlled delivery of bioactive molecules to modulate or control biological processes has a number of clinical applications. The present study reports a delivery system which was designed to deliver growth factors locally to a fracture site. Ethylene vinyl acetate copolymer (EVAc), bovine serum albumin (BSA) and platelet-derived growth factor (PDGF-BB) were combined and coated onto a stainless-steel Kirschner wire (K-wire). A K-wire can be used as an intramedullary nail in small animal fractures, such as the rat. PDGF-BB stimulates thymidine uptake in human bone cell cultures when released from the K-wire delivery system. BSA release was modified by providing a final coating on the K-wire of 10% pure EVAc at the end of the fabrication. Electron microscopic examination of the surface of the rods revealed different surface pores on the K-wires coated with pure EVAc. Differences in porosity and tortuosity may account, in part, for the different release kinetics observed.






Walsh WR,Kim HD,Jong YS,Valentini RF




Has Abstract


1995-11-01 00:00:00














  • Effect of resin hydrophilicity and temperature on water sorption of dental adhesive resins.

    abstract::This study examined the effects of copolymer hydrophilicity and temperature on water sorption and solubility characteristics of five copolymer blends of increasing degree of hydrophilicity using gravimetric measurements. Six resin disks (15 mm in diameter x 1 mm in thickness) were prepared from each copolymer blend an...


    pub_type: 杂志文章


    authors: Yiu CK,King NM,Carrilho MR,Sauro S,Rueggeberg FA,Prati C,Carvalho RM,Pashley DH,Tay FR

    更新日期:2006-03-01 00:00:00

  • Erosion of composite polymer matrices.

    abstract::The erosion of composite polymer matrices made of slow and fast eroding polymers was investigated. These matrices can be used as implants that release drugs in a preprogrammed way. To understand the mechanism of drug release, the erosion of cylindrical polymer matrices made of several layers of different polymers was ...


    pub_type: 杂志文章


    authors: Göpferich A

    更新日期:1997-03-01 00:00:00

  • Targeting fibronectins of glioma extracellular matrix by CLT1 peptide-conjugated nanoparticles.

    abstract::The abundant extracellular matrix (ECM) in the glioma microenvironment play a critical role in the maintenance of glioma morphology, glioma cells differentiation and proliferation, but little has been done to understand the feasibility of ECM as the therapeutic target for glioma therapy. In this study, a drug delivery...


    pub_type: 杂志文章


    authors: Zhang B,Shen S,Liao Z,Shi W,Wang Y,Zhao J,Hu Y,Yang J,Chen J,Mei H,Hu Y,Pang Z,Jiang X

    更新日期:2014-04-01 00:00:00

  • Spinal fusion surgery: animal models for tissue-engineered bone constructs.

    abstract::Animal models have been used extensively to investigate the biology of fracture healing and spinal fusion. The goal of each spinal fusion model is to try and reproduce the correct sequence of events during osseous healing in humans. Animal models allow us the capability of dialing in fusion rates and fusion parameters...


    pub_type: 杂志文章,评审


    authors: Khan SN,Lane JM

    更新日期:2004-04-01 00:00:00

  • Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition.

    abstract::Construction of biodegradable, three-dimensional scaffolds for tissue engineering has been previously described using a variety of molding and rapid prototyping techniques. In this study, we report and compare two methods for fabricating poly(DL-lactide-co-glycolide) (PLGA) scaffolds with feature sizes of approximatel...


    pub_type: 杂志文章


    authors: Vozzi G,Flaim C,Ahluwalia A,Bhatia S

    更新日期:2003-06-01 00:00:00

  • Cell adhesion to silicon substrata: characterization by means of optical and acoustic cytometric techniques.

    abstract::The adhesion and growth of cells on silicon dioxide substrata are analysed. The adhesion of human red blood cells to silicon dioxide layers of different depth and doping compares well with that to glass. Rat pheochromocytoma cells also grow on the same substrata. The adhesion of these two biological systems is charact...


    pub_type: 杂志文章


    authors: Grattarola M,Tedesco M,Cambiaso A,Perlo G,Giannetti G,Sanguineti A

    更新日期:1988-01-01 00:00:00

  • Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells.

    abstract::The performance of implantable biomaterials derived from decellularized tissue, including encouraging results with skeletal muscle, suggests that the extracellular matrix (ECM) derived from native tissue has promising regenerative potential. Yet, the supply of biomaterials derived from donated tissue will always be li...


    pub_type: 杂志文章


    authors: Hurd SA,Bhatti NM,Walker AM,Kasukonis BM,Wolchok JC

    更新日期:2015-05-01 00:00:00

  • SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics.

    abstract::Surface plasmon resonance (SPR) sensing has long been used to study biomolecular binding events and their kinetics in a label-free way. This approach has recently been extended to SPR microscopy, which is an ideal tool for probing large microarrays of biomolecules for their binding interactions with various partners a...


    pub_type: 杂志文章,评审


    authors: Campbell CT,Kim G

    更新日期:2007-05-01 00:00:00

  • MLV based viral-like-particles for delivery of toxic proteins and nuclear transcription factors.

    abstract::We have developed nanoparticles based on Murine Leukemia Virus virus-like-particles (VLPs) that efficiently deliver therapeutic bioactive proteins in their native state into target cells. Nuclear transcription factors and toxic proteins were incorporated into the VLPs from stable producer cells without transducing vir...


    pub_type: 杂志文章


    authors: Wu DT,Roth MJ

    更新日期:2014-09-01 00:00:00

  • Encapsulated cells: an atomic force microscopy study.

    abstract::Two types of cells--human platelets and spore cells--were encapsulated in polymer shells by adsorbing polyanions and polycations in a stepwise fashion. The encapsulated cells were attached to gold and silicon surfaces and their morphological and adhesion properties were studied in air using tapping mode atomic force m...


    pub_type: 杂志文章


    authors: Yu M,Ivanisevic A

    更新日期:2004-08-01 00:00:00

  • Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

    abstract::Given that vascular endothelial growth factor (VEGF) and placental growth factor (PIGF), over-expressed in breast cancer cells and M2-like tumor-associated macrophages (M2-TAMs) within tumor microenvironment (TME), work synergistically and independently in mediating tumor progression and immunosuppression, combinatori...


    pub_type: 杂志文章


    authors: Song Y,Tang C,Yin C

    更新日期:2018-12-01 00:00:00

  • Integrins as linker proteins between osteoblasts and bone replacing materials. A critical review.

    abstract::The adhesion of osteoblasts to substrates is mediated through proteins that have adsorbed to the substrate, providing integrins on the cell membrane with ligands to connect to. The integrins regulate cell behavior through bi-directional signaling pathways. This critical review has the purpose to consider the research ...


    pub_type: 杂志文章,评审


    authors: Siebers MC,ter Brugge PJ,Walboomers XF,Jansen JA

    更新日期:2005-01-01 00:00:00

  • Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography.

    abstract::Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical sc...


    pub_type: 杂志文章


    authors: Levitz D,Hinds MT,Ardeshiri A,Hanson SR,Jacques SL

    更新日期:2010-11-01 00:00:00

  • Preparation and in vitro evaluation of porous silica gels.

    abstract::Porous silica gels with high surface areas were prepared from tetraethylothosilicate and polyacrylic acid (PAA) of high molecular weight in acidic media by a sol-gel method. PAA content and ageing temperatures were varied in order to obtain different microstructures. Samples were sintered at temperatures up to 400 deg...


    pub_type: 杂志文章


    authors: Gallardo J,Galliano PG,Porto López JM

    更新日期:2002-11-01 00:00:00

  • Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds.

    abstract::Herein we describe a bio-inspired, affinity binding alginate-sulfate scaffold, designed for the presentation and sustained release of transforming growth factor beta 1 (TGF-β1), and examine its effects on the chondrogenesis of human mesenchymal stem cells (hMSCs). When attached to matrix via affinity interactions with...


    pub_type: 杂志文章


    authors: Re'em T,Kaminer-Israeli Y,Ruvinov E,Cohen S

    更新日期:2012-01-01 00:00:00

  • In vivo studies of silk based gold nano-composite conduits for functional peripheral nerve regeneration.

    abstract::We report a novel silk-gold nanocomposite based nerve conduit successfully tested in a neurotmesis grade sciatic nerve injury model in rats over a period of eighteen months. The conduit was fabricated by adsorbing gold nanoparticles onto silk fibres and transforming them into a nanocomposite sheet by electrospinning w...


    pub_type: 杂志文章


    authors: Das S,Sharma M,Saharia D,Sarma KK,Sarma MG,Borthakur BB,Bora U

    更新日期:2015-09-01 00:00:00

  • Processes human umbilical veins as arterial substitutes--evaluation in canine models.

    abstract::Human umbilical cord vein segments have been used as vessel substitutes for damaged or occluded arteries, as aorto-coronary by-passes and as arterio-venous fistulae for dialysis. The Dardik-Biograft fixed with glutaraldehyde and the Mindich-Bioflow, fixed with ethanol and dialdehyde starch, are commercially available....


    pub_type: 杂志文章


    authors: Guidoin R,Marois M,Martin L,Noël HP,Laroche F,Gosselin CO,Côté R,Bénichoux R,Blais P

    更新日期:1980-04-01 00:00:00

  • Sustained delivery of growth factors from methylidene malonate 2.1.2-based polymers.

    abstract::The incorporation of growth factors into new methylidene malonate 2.1.2-based biocompatible polymeric blends of oligomers and polymers to improve their stability and controlled release was investigated. Five growth factors were used in this study: FGF2, PDGF, TGF-beta, NGF and GM-CSF. Formulation in poly(methylidene m...


    pub_type: 杂志文章


    authors: Désiré L,Mysiakine E,Bonnafous D,Couvreur P,Sagodira S,Breton P,Fattal E

    更新日期:2006-04-01 00:00:00

  • Understanding the role of tissue-specific decellularized spinal cord matrix hydrogel for neural stem/progenitor cell microenvironment reconstruction and spinal cord injury.

    abstract::The repair of spinal cord injury (SCI) highly relies on microenvironment remodeling and facilitating the recruitment and neuronal differentiation of endogenous stem/progenitor cells. Decellularized tissue matrices (DTMs) have shown their unique and beneficial characteristics in promoting neural tissue regeneration, es...


    pub_type: 杂志文章


    authors: Xu Y,Zhou J,Liu C,Zhang S,Gao F,Guo W,Sun X,Zhang C,Li H,Rao Z,Qiu S,Zhu Q,Liu X,Guo X,Shao Z,Bai Y,Zhang X,Quan D

    更新日期:2021-01-01 00:00:00

  • Poly(ether-ether-ketone) orthopedic bearing surface modified by self-initiated surface grafting of poly(2-methacryloyloxyethyl phosphorylcholine).

    abstract::We investigated the production of free radicals on a poly(ether-ether-ketone) (PEEK) substrate under ultraviolet (UV) irradiation. The amount of the ketyl radicals produced from the benzophenone (BP) units in the PEEK molecular structure initially increased rapidly and then became almost constant. Our observations rev...


    pub_type: 杂志文章


    authors: Kyomoto M,Moro T,Yamane S,Hashimoto M,Takatori Y,Ishihara K

    更新日期:2013-10-01 00:00:00

  • MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia.

    abstract::microRNA-124 (miR-124), the most abundant miRNA of the CNS, was recently shown to modulate the polarization of activated microglia and infiltrating macrophages towards the anti-inflammatory M2 phenotype and protect neurons in various ways after brain disease. In ischemic stroke, microglia and macrophages of a detrimen...


    pub_type: 杂志文章


    authors: Hamzei Taj S,Kho W,Riou A,Wiedermann D,Hoehn M

    更新日期:2016-06-01 00:00:00

  • Structurally engineered anodic alumina nanotubes as nano-carriers for delivery of anticancer therapeutics.

    abstract::Here, we report a study on the biocompatibility, cell uptake and in vitro delivery of tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) by new nano-carriers called anodic alumina nanotubes (AANTs) for potential cancer therapy. AANTs were electrochemically engineered by a unique pulse anodization pr...


    pub_type: 杂志文章


    authors: Wang Y,Santos A,Kaur G,Evdokiou A,Losic D

    更新日期:2014-07-01 00:00:00

  • A microwell array system for stem cell culture.

    abstract::Directed embryonic stem (ES) cell differentiation is a potentially powerful approach for generating a renewable source of cells for regenerative medicine. Typical in vitro ES cell differentiation protocols involve the formation of ES cell aggregate intermediates called embryoid bodies (EBs). Recently, we demonstrated ...


    pub_type: 杂志文章


    authors: Moeller HC,Mian MK,Shrivastava S,Chung BG,Khademhosseini A

    更新日期:2008-02-01 00:00:00

  • Tumor-activatable ultrasmall nanozyme generator for enhanced penetration and deep catalytic therapy.

    abstract::Tumor-activatable ultrasmall nanozyme generation is an unprecedented strategy to overcome intrinsically fatal defects of traditional reactive oxygen species (ROS)-based nanoagents for deep tumor penetration, including limited tissue-penetrating depth of external energy, heavy reliance on oxygen and nonspecific toxicit...


    pub_type: 杂志文章


    authors: Liu X,Liu Z,Dong K,Wu S,Sang Y,Cui T,Zhou Y,Ren J,Qu X

    更新日期:2020-11-01 00:00:00

  • In vitro and in vivo calcification of vascular bioprostheses.

    abstract::Efficacy of different chemical treatments on calcification of vascular graft in vitro and in vivo was studied. Culture medium-filled rat aortas were separately treated in 0.2% glutaraldehyde and epoxy compound, and photooxidized in 0.01% methylene blue for a shorter period (group 1). Another group of rat aortas were s...


    pub_type: 杂志文章


    authors: Chanda J,Kondoh K,Ijima K,Matsukawa M,Kuribayashi R

    更新日期:1998-09-01 00:00:00

  • The role of trabecular demineralized bone in combination with perichondrium in the generation of cartilage grafts.

    abstract::The use of a composite graft of bovine trabecular demineralized bone matrix (DBM) and perichondrium has been found a reliable method for in vivo generation of cartilage. In the present study, the mechanism whereby this commercially available matrix increases cartilage formation was investigated. First, the time course...


    pub_type: 杂志文章


    authors: van Osch GJ,ten Koppel PG,van der Veen SW,Poppe P,Burger EH,Verwoerd-Verhoef HL

    更新日期:1999-02-01 00:00:00

  • A multifunctional nanotheranostic for the intelligent MRI diagnosis and synergistic treatment of hypoxic tumor.

    abstract::Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for ...


    pub_type: 杂志文章


    authors: Song R,Zhang M,Liu Y,Cui Z,Zhang H,Tang Z,Chen X,Wu H,Yao Z,He M,Bu W

    更新日期:2018-08-01 00:00:00

  • Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking.

    abstract::Artificial extracellular matrix proteins, genetically engineered from elastin- and fibronectin-derived repeating units, were crosslinked with hexamethylene diisocyanate in dimethylsulfoxide. The resulting hydrogel films were transparent, uniform, and highly extensible. Their tensile moduli depended on crosslinker conc...


    pub_type: 杂志文章


    authors: Nowatzki PJ,Tirrell DA

    更新日期:2004-03-01 00:00:00

  • Optimized phospholipid-based nanoparticles for inner ear drug delivery and therapy.

    abstract::To develop efficient carriers for inner ear drug delivery, we prepared four kinds of phospholipid-based nanoparticles: neutral, anionic, cationic, and cationic-PEG (polyethyleneglycol) particles. PEG was used to maintain long-term particle circulation in the perilymph, avoiding non-specific binding of particles to pro...


    pub_type: 杂志文章


    authors: Yang KJ,Son J,Jung SY,Yi G,Yoo J,Kim DK,Koo H

    更新日期:2018-07-01 00:00:00

  • Constitutive models for impacted morsellised cortico-cancellous bone.

    abstract::Constitutive models are developed, based on the results of confined compression testing, to describe the visco-elastic, and non-linear elasto-plastic behaviour of morsellised cortico-cancellous bone (MCB). It is found that the elastic modulus, E of MCB can be expressed as a linear function of the applied pressure, p. ...


    pub_type: 杂志文章


    authors: Phillips A,Pankaj P,May F,Taylor K,Howie C,Usmani A

    更新日期:2006-03-01 00:00:00