Benzoyl peroxide cytotoxicity evaluated in vitro with the human keratinocyte cell line, RHEK-1.


:The human keratinocyte cell line, RHEK-1, was used to evaluate the cytotoxicity of benzoyl peroxide (BZP). As determined with the neutral red (NR) cytotoxicity assay, the 24-h midpoint (NR50) toxicity values, in mM, were 0.11 for BZP and 29.5 for benzoic acid, the stable metabolite of BZP. Irreversible cytotoxicity occurred after a 1-h exposure to 0.15 mM BZP and greater. When exposed to BZP for 7 days, a lag in growth kinetics was first observed at 0.06 mM BZP. Damage to the integrity of the plasma membrane was evident, as leakage of lactic acid dehydrogenase occurred during a 4-h exposure to BZP at 0.05 mM and greater. Intracellular membranes were also affected, as extensive vacuolization, initially perinuclear but then spreading throughout the cytoplasm, was noted in BZP-stressed cells. The generation of reactive free radicals from BZP was suggested by the following: the intracellular content of glutathione was lowered in cells exposed to BZP; cells pretreated with the glutathione-depleting agent, chlorodinitrobenzene, were hypersensitive to a subsequent challenge with BZP; lipid peroxidation by BZP was inducible in the presence of Fe2+; and cells previously maintained in a medium amended with vitamin E, an antioxidant, were more resistant to BZP, showed less lipid peroxidation in the presence of BZP+Fe2+ and did not develop the extensive intracellular vacuolization as compared to non-vitamin E maintained cells.






Babich H,Zuckerbraun HL,Wurzburger BJ,Rubin YL,Borenfreund E,Blau L




Has Abstract


1996-01-08 00:00:00














  • Analytical challenges for conducting rapid metabolism characterization for QIVIVE.

    abstract::For quantitative in vitro-in vivo extrapolation (QIVIVE) of metabolism for the purposes of toxicokinetics prediction, a precise and robust analytical technique for identifying and measuring a chemical and its metabolites is an absolute prerequisite. Currently, high-resolution mass spectrometry (HR-MS) is a tool of cho...


    pub_type: 杂志文章,评审


    authors: Tolonen A,Pelkonen O

    更新日期:2015-06-05 00:00:00

  • Ecological risk assessment and testing for endocrine disruption in the aquatic environment.

    abstract::Field observations of endocrine disruption (ED) in fish and other aquatic species have contributed to a global effort to establish test methods for detecting ED effects in wildlife species. For ecological risk assessment, validated tests are needed for Amphibia, fish and aquatic invertebrates, supported by a tiered ap...


    pub_type: 杂志文章,评审


    authors: Hutchinson TH,Pickford DB

    更新日期:2002-12-27 00:00:00

  • Up-regulation of macrophage inflammatory protein-2 and complement 3A receptor by the trichothecenes deoxynivalenol and satratoxin G.

    abstract::The trichothecenes are a group of mycotoxins that target leukocytes and have a wide range of immunomodulatory effects. Differential display analysis was applied to assess the effects of the trichothecenes deoxynivalenol (vomitoxin, DON) and satratoxin G (SG), on mRNA in the RAW 264.7 macrophage cell line. Cells were i...


    pub_type: 杂志文章


    authors: Chung YJ,Yang GH,Islam Z,Pestka JJ

    更新日期:2003-04-15 00:00:00

  • An assessment of the neurotoxic potential of fenitrothion in the hen.

    abstract::The potential of single, toxic doses of fenitrothion (O,O-dimethyl O-(4-nitro-m-tolyl)phosphorothioate) to elicit delayed neurotoxicity in the adult White Leghorn hen was compared to the effects produced following similar treatment with the known neurotoxin, tri-o-tolyl phosphate (TOTP). Hens (2.0-2.5 kg body wt) rece...


    pub_type: 杂志文章


    authors: Durham HD,Ecobichon DJ

    更新日期:1986-11-01 00:00:00

  • Synthesis and utilization of neurotransmitters: actions of subconvulsant doses of hexachlorocyclohexane isomers on brain monoamines.

    abstract::We have developed a model for the treatment of data of concentration of brain neurotransmitters (particularly serotonin and the catecholamines), in which the changes induced by any given treatment on the neurotransmitter (NT) and its main metabolite (ME) are converted into 2 new parameters named S and U, that are rela...


    pub_type: 杂志文章


    authors: Artigas F,Martínez E,Camón L,Rodríguez Farré E

    更新日期:1988-04-01 00:00:00

  • Effect of glucuronidation substrates/inhibitors on N-(3,5-dichlorophenyl)succinimide nephrotoxicity in Fischer 344 rats.

    abstract::The agricultural fungicide N-(3,5-dichlorophenyl)succinimide (NDPS) is an acute nephrotoxicant in rats. Our previous studies have strongly suggested that glucuronide conjugation of NDPS metabolites might be a bioactivation step mediating NDPS nephrotoxicity. In this study, effects of substrates and/or inhibitors of pr...


    pub_type: 杂志文章


    authors: Hong SK,Anestis DK,Brown PI,Rankin GO

    更新日期:1999-01-01 00:00:00

  • Homologous recombination and cell cycle checkpoints: Rad51 in tumour progression and therapy resistance.

    abstract::We provide an overview of the functional interrelationship between genes and proteins related to DNA repair by homologous recombination and cell cycle regulation in relation to the progression and therapy resistance of human tumours. To ensure the high-fidelity transmission of genetic information from one generation t...


    pub_type: 杂志文章,评审


    authors: Henning W,Stürzbecher HW

    更新日期:2003-11-15 00:00:00

  • Delta(9)-Tetrahydrocannabinol enhances MCF-7 cell proliferation via cannabinoid receptor-independent signaling.

    abstract::We recently reported that Delta(9)-tetrahydrocannabinol (Delta(9)-THC) has the ability to stimulate the proliferation of human breast carcinoma MCF-7 cells. However, the mechanism of action remains to be clarified. The present study focused on the relationship between receptor expression and the effects of Delta(9)-TH...


    pub_type: 杂志文章


    authors: Takeda S,Yamaori S,Motoya E,Matsunaga T,Kimura T,Yamamoto I,Watanabe K

    更新日期:2008-03-12 00:00:00

  • Different levels of Schistosoma mansoni infection increased the mutagenicity of benzo(a)pyrene, the activity of aryl hydrocarbon hydroxylase and the formation of hepatic microsomal hydrogen peroxide.

    abstract::The present study investigates the influence of different levels of Schistosoma mansoni infection (60, 120, 180, 300, 600 cercariae per mice) after 33 days on the activity of aryl hydrocarbon hydroxylase (AHH) and the formation of hepatic hydrogen peroxide (H(2)O(2)) during the metabolic activation of benzo(a)pyrene [...


    pub_type: 杂志文章


    authors: Awney HA,Ghzlan HE,Sheweita SA,Mostafa MH

    更新日期:2001-06-21 00:00:00

  • Effects of S-adenosylmethionine on intrabiliary glutathione degradation induced by long-term administration of cyclosporin A in the rat.

    abstract::We investigate the ability of S-adenosylmethionine (SAMe) to antagonize the cyclosporine A (CyA)-induced inhibition of biliary glutathione efflux induced by long-term administration of CyA (10 mg/kg per day-CyA(10) or 20 mg/kg per day-CyA(20) for 4 weeks) in rats. CyA treatment reduced the liver content of total gluta...


    pub_type: 杂志文章


    authors: Palomero J,Galán AI,Muñoz ME,González-Gallego J,Tuñón MJ,Jiménez R

    更新日期:2004-07-15 00:00:00

  • Detection of systemic hypersensitivity to drugs using standard guinea pig assays.

    abstract::The most commonly used assays designed to detect either skin or systemic immune-based hypersensitivity reactions are those using guinea pigs (GP). We obtained data from various FDA records to evaluate the correlation between GP assay results and reported post-marketing systemic hypersensitivity reactions. We examined ...


    pub_type: 杂志文章,评审


    authors: Weaver JL,Staten D,Swann J,Armstrong G,Bates M,Hastings KL

    更新日期:2003-12-01 00:00:00

  • Uptake of carbon monoxide in blood of miniture pigs and other mammals.

    abstract::Different affinities of blood for CO were observed in in vivo and in vitro experiments. Hamsters showed the greatest relative affinity (1.0), the corresponding value for rats was 0.8, for pigs 0.74 and for rabbits 0.58. The upward shift of the saturation curves was dependent on the rate of respiratory exchange per uni...


    pub_type: 杂志文章


    authors: Klimisch HJ,Chevalier HJ,Harke HP,Dontenwill W

    更新日期:1975-01-01 00:00:00

  • The selective cytotoxicity of the alkenyl glucosinolate hydrolysis products and their presence in Brassica vegetables.

    abstract::Cruciferous vegetable consumption correlates with reduced risk of cancer. This chemopreventative activity may involve glucosinolates and their hydrolysis products. Glucosinolate-derived isothiocyanates have been studied for their toxicity and chemopreventative properties, but other hydrolysis products (epithionitriles...


    pub_type: 杂志文章


    authors: Kadir NH,David R,Rossiter JT,Gooderham NJ

    更新日期:2015-08-06 00:00:00

  • The role of thiols, dithiols, nutritional factors and interacting ligands in the toxicology of mercury.

    abstract::Mercury has been a known as a toxic substance for centuries. Whilst the clinical features of acute mercury poisoning have been well described, chronic low dose exposure to mercury remains poorly characterised and its potential role in various chronic disease states remains controversial. Low molecular weight thiols, i...


    pub_type: 杂志文章,评审


    authors: Rooney JP

    更新日期:2007-05-20 00:00:00

  • Increased levels of metallothionein in placenta of smokers.

    abstract::Experiments were designed to evaluate and compare metallothionein (MT), zinc and cadmium levels in human placentas of smoking and non-smoking women. Smoking was assessed by self-reported cigarette consumption and urine cotinine levels before delivery. Smoking pregnant women with urine cotinine levels higher than 130 n...


    pub_type: 临床试验,杂志文章


    authors: Ronco AM,Arguello G,Suazo M,Llanos MN

    更新日期:2005-03-01 00:00:00

  • In vivo and in vitro toxicity of newly synthesized monofunctional sulfur mustard derivatives.

    abstract::The toxicity of two new monofunctional sulfur mustard derivatives was tested. The compound (4-carboxybutyl 2-chloroethyl sulfide, CBCS; 10-carboxydecyl 2-chloroethyl sulfide, CDCS) possess the 2-chloroethyl sulfide moiety present in mustard gas. Exposure of guinea pig skin to CBCS resulted in a dose-related ulcerative...


    pub_type: 杂志文章


    authors: Wormser U,Green BS,Arad-Yellin R,Brodsky B,Shatz I,Nyska A

    更新日期:1996-04-15 00:00:00

  • Endoplasmic reticulum stress and MAPK signaling pathway activation underlie leflunomide-induced toxicity in HepG2 Cells.

    abstract::Leflunomide, used for the treatment of rheumatoid arthritis, has been reported to cause severe liver problems and liver failure; however, the underlying mechanisms are not clear. In this study, we used multiple approaches including genomic analysis to investigate and characterize the possible molecular mechanisms of t...


    pub_type: 杂志文章


    authors: Ren Z,Chen S,Qing T,Xuan J,Couch L,Yu D,Ning B,Shi L,Guo L

    更新日期:2017-12-01 00:00:00

  • Oxidative stress and cytotoxicity of 4-(2-thienyl)butyric acid in isolated rat renal proximal tubular and distal tubular cells.

    abstract::Relative cytotoxicity and mechanism of action of thiophenes were investigated in suspensions of freshly isolated renal proximal tubular (PT) and distal tubular (DT) cells using 4-(2-thienyl)butyric acid (TByA) as a model compound. TByA produced significantly greater time- and concentration-dependent cytotoxicity, as a...


    pub_type: 杂志文章


    authors: Lash LH,Tokarz JJ

    更新日期:1995-12-10 00:00:00

  • The effects of some redox-active metals and reactive aldehydes on DNA-protein cross-links in vitro.

    abstract::It has been suggested that the measurement of DNA-protein cross-links (DPCs) may be of value in the assessment of an individual's exposure to specific environmental insults. For a biomarker to be reliable, its results should be consistent and specific. In the present study, the precision and specificity of the K(+)-SD...


    pub_type: 杂志文章


    authors: Olin KL,Cherr GN,Rifkin E,Keen CL

    更新日期:1996-06-17 00:00:00

  • Wnt family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice.

    abstract::Proliferation and differentiation of cells in female reproductive organs, the oviduct, uterus and vagina, are regulated by endogenous estrogen. In utero exposure to a synthetic estrogen, diethylstilbestrol (DES), induces vaginal clear-cell adenocarcinoma in humans. In mice, perinatal exposure to DES results in abnorma...


    pub_type: 杂志文章


    authors: Nakamura T,Miyagawa S,Katsu Y,Watanabe H,Mizutani T,Sato T,Morohashi K,Takeuchi T,Iguchi T,Ohta Y

    更新日期:2012-06-14 00:00:00

  • The effect of initiating doses of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene on the expression of PAH activating enzymes and its modulation by plant phenols.

    abstract::Polycyclic aromatic hydrocarbons (PAHs) including benzo[a]pyrene (B[a]P) and 7,12-dimethylbenz[a]anthracene (DMBA) are environmental pollutants, which undergo metabolic activation to exert their carcinogenic effects. Our earlier studies showed that naturally occurring plant phenols, protocatechuic, chlorogenic, tannic...


    pub_type: 杂志文章


    authors: Szaefer H,Krajka-Kuźniak V,Baer-Dubowska W

    更新日期:2008-09-29 00:00:00

  • Selective pancreatic toxicity of palmitoylpentachlorophenol.

    abstract::Palmitoylpentachlorophenol (PPCP), which is a lipid conjugate of a xenobiotic compound, has been found in human fat. To study the toxicity associated with PPCP, rats were given 100 mg/kg PPCP and sacrificed at 4, 8 and 12 days. The target organ identified was the exocrine pancreas; no other major organs examined showe...


    pub_type: 杂志文章


    authors: Ansari GA,Kaphalia BS,Boor PJ

    更新日期:1987-10-12 00:00:00

  • Receptor-dependent regulation of the CYP3A4 gene.

    abstract::A CYP3A4 promoter-reporter gene construct has been used to assess the ability of 16 known (in vivo) and putative (in vitro) inducers to transactivate a CYP3A4 reporter gene in HepG2 cells. With the exception of pravastatin, the remaining 15 compounds transactivated the CYP3A4 reporter gene with differing inductive abi...


    pub_type: 杂志文章


    authors: Gibson GG,el-Sankary W,Plant NJ

    更新日期:2002-12-27 00:00:00

  • Ferrous ion-stimulated alkane expiration in rats treated with carbon tetrachloride.

    abstract::Rats which were treated intraperitoneally (i.p.) with FeCl2 X 4H2O expired ethane which increased with increasing doses of iron. The same amount of ethane was expired by animals exposed to 20% or 100% oxygen. The mobilization by FeCl2 of stored ethane from the body was excluded. Propane expiration was also stimulated ...


    pub_type: 杂志文章


    authors: Muliawan H,Kappus H

    更新日期:1983-09-01 00:00:00

  • Disposition and metabolism of [14C]1,2-dichloropropane following oral and inhalation exposure in Fischer 344 rats.

    abstract::The objective of this study was to compare the disposition and metabolism of [14C]1,2-dichloropropane [( 14C]DCP) following oral and inhalation exposure since these two routes are of interest with regards to occupational and accidental exposure. [14C]DCP was administered orally to groups of four rats of each sex as a ...


    pub_type: 杂志文章


    authors: Timchalk C,Dryzga MD,Smith FA,Bartels MJ

    更新日期:1991-01-01 00:00:00

  • DNA methylation in cardiac fibrosis: new advances and perspectives.

    abstract::Cardiac fibrosis is characterized by net accumulation of extracellular matrix (ECM) proteins in the cardiac interstitium, and contributes to both systolic and diastolic dysfunction in many cardiac pathophysiologic conditions. More specifically, cardiac fibroblasts are activated by a variety of pathological stimuli, th...


    pub_type: 杂志文章,评审


    authors: Tao H,Yang JJ,Shi KH,Deng ZY,Li J

    更新日期:2014-09-02 00:00:00

  • [Effects of methylcyclopentadienyl manganese tricarbonyl (MMT) on rat liver mitochondria. II. Activity-structure relation (author's transl)].

    abstract::The action of various manganese organic compounds, which are structural analogs of methylcyclopentadienyl manganese tricarbonyl (MMT), was investigated. Only the cyclopentadienyl manganese tricarbonyl compounds are effective inhibitors of mitochondrial respiration, but only when associated with NAD+-linked substrates....


    pub_type: 杂志文章


    authors: Autissier N,Gautheron B,Dumas P,Brosseau J,Loireau A

    更新日期:1977-10-01 00:00:00

  • Melamine impairs spatial cognition and hippocampal synaptic plasticity by presynaptic inhibition of glutamatergic transmission in infant rats.

    abstract::The scandal of melamine-contamination has not been quite blown out, since the toxicity of melamine continues to raise concerns for public health. It has been well known that fetus and infant periods play the most important roles in brain development, whereas little has been done on the harmful effects of melamine on t...


    pub_type: 杂志文章


    authors: Yang J,An L,Yao Y,Yang Z,Zhang T

    更新日期:2011-11-18 00:00:00

  • Protection in the late stages of paracetamol-induced liver cell injury with fructose, cyslosporin A and trifluoperazine.

    abstract::A fixed combination of the three components, fructose, cyclosporin A and trifluoperazine (FCAT), was found to protect in the late stage of paracetamol-induced liver cell injury both in vivo and in an in vivo/in vitro system. Rats pre-induced with phenobarbitone were given a paracetamol dose of 1 g/kg i.p. The combinat...


    pub_type: 杂志文章


    authors: Beales D,McLean AE

    更新日期:1996-03-18 00:00:00

  • Modulation of Delta9-tetrahydrocannabinol-induced MCF-7 breast cancer cell growth by cyclooxygenase and aromatase.

    abstract::Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), a major constituent of marijuana, has been shown to stimulate the growth of MCF-7 breast cancer cells through cannabinoid receptor-independent signaling [Takeda, S., Yamaori, S., Motoya, E., Matsunaga, T., Kimura, T., Yamamoto, I., Watanabe, K., 2008. Delta(9)-Tetrahydroca...


    pub_type: 杂志文章


    authors: Takeda S,Yamamoto I,Watanabe K

    更新日期:2009-05-02 00:00:00