Abstract:
AIMS:To evaluate an indirect immunofluorescence flow cytometry technique in a series of patients with large fetomaternal haemorrhage (FMH). METHODS:Patient samples identified by Kleihauer testing in local laboratories as having FMH > 4 ml were sent for flow cytometric analysis. In a proportion of cases the mothers received anti-D immunoglobulin prophylaxis according to the flow cytometer estimate of FMH volume. RESULTS:Forty three cases of FMH were studied prospectively. The correlation between Kleihauer and flow cytometry results was poor. In 38 (88%) cases the size of FMH quantitated by flow cytometry was lower than that estimated using the Kleihauer technique. In 13 (30%) cases no Rh D immunoglobulin positive cells were detected by flow cytometry. Centralised review of the original Kleihauer films using a calibrated microscope resulted in improved, but still suboptimal correlation with flow cytometry results. In 15 cases anti-D immunoglobulin was given according to the flow cytometer estimation of FMH size, resulting in a 58% reduction in the amount of anti-D immunoglobulin given. None of the patients were immunised when tested six months later. CONCLUSIONS:Flow cytometry is helpful for the accurate quantitation and management of patients with large FMH and in cases where the presence of maternal haemoglobin F containing cells renders the Kleihauer technique inaccurate. Worthwhile reductions in the use of anti-D immunoglobulin can be achieved.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Johnson PR,Tait RC,Austin EB,Shwe KH,Lee Ddoi
10.1136/jcp.48.11.1005subject
Has Abstractpub_date
1995-11-01 00:00:00pages
1005-8issue
11eissn
0021-9746issn
1472-4146journal_volume
48pub_type
临床试验,杂志文章abstract::Visceral botryomycosis is rare, and documented sites are lung, brain, kidney, liver and prostate. This report describes a rare autopsy case of disseminated visceral botryomycosis, with bulky, grape-like botryomycotic vegetations in the heart, and similar abscesses in the lungs and bone marrow. This is the first such r...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2008.057588
更新日期:2008-08-01 00:00:00
abstract:AIMS:To identify calcifying epithelial odontogenic tumour (CEOT) mutations in oncogenes and tumour suppressor genes. METHODS:A panel of 50 genes commonly mutated in cancer was sequenced in CEOT by next-generation sequencing. Sanger sequencing was used to cover the region of the frameshift deletion identified in one sa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2017-204813
更新日期:2018-03-01 00:00:00
abstract::A sensitive gas chromatography-mass spectrometric method was used to measure the generation in whole blood of leukotriene B4 (LTB4), a potent stimulator of neutrophil chemotaxis, in eight patients with chronic granulocytic leukaemia (CGL) and 12 healthy controls. LTB4 was detectable in unstimulated samples from all th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.11.1163
更新日期:1988-11-01 00:00:00
abstract:AIMS:To validate a method of assessment of low hepatic iron concentration based on a biochemical colorimetric assay plus histological scoring. METHODS:The within-day and day to day precision of the iron colorimetric assay was determined on frozen rat liver. The coefficient of variation (CV) of iron measurement in two ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.6.430
更新日期:1999-06-01 00:00:00
abstract::Metabolic balance studies for iron have been carried out on 24 apparently healthy elderly people (11 men and 13 women) aged 69.7 to 85.5 years living in their own homes and eating self selected diets. Several biochemical and haematological indices of iron state were also measured. The mean daily iron intake was 176 mu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.12.1353
更新日期:1984-12-01 00:00:00
abstract::Rapid diagnoses were made on 510 breast lumps by three methods in sequence and the results compared with those obtained by paraffin histology.Naked-eye examination of the excised lumps correctly distinguished between benign and malignant tumours in 95.1% of cases with 23 (4.5%) false negative and two (0.4%) false posi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.4.273
更新日期:1973-04-01 00:00:00
abstract:AIMS:To investigate the role of needle core biopsy (NCB) in the preoperative assessment of impalpable breast lesions, mainly derived from the NHS Breast Screening Programme (NHSBSP) and to assess our own modifications to a suggested system for the classification of breast NCBs. METHODS:The NCB, fine needle aspiration ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.2.121
更新日期:2001-02-01 00:00:00
abstract::Two groups of women with non-specific genital infection were treated for two weeks with tetracycline 250 mg six-hourly and minocycline 100 mg twice daily respectively. Cultures for yeasts were performed before treatment and at the end of the first and second weeks. Before treatment yeasts, mostly Candida albicans, wer...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.28.5.403
更新日期:1975-05-01 00:00:00
abstract::Specimens from 95 patients attending a venereal diseases clinic were examined for gonococci by three methods--a conventional culture technique using modified Thayer-Martin medium, microscopy of a Gram-stained direct smear, and the Microcult system. For 56% of the specimens the results by all three methods agreed. Assu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.3.209
更新日期:1978-03-01 00:00:00
abstract::The biochemical classification of the Klebsiella proposed by Cowan, Steel, Shaw, and Duguid (1960) has been applied to 50 strains isolated as the pure or predominant growth from sputum, and Klebsiella species have been correlated with the severity of the chest infection in the patient. A modification in nomenclature i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.5.478
更新日期:1962-09-01 00:00:00
abstract:AIMS:This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia. METHODS:Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202668
更新日期:2015-03-01 00:00:00
abstract::The use of liquid media to detect the production of beta-lactamase by beta-lactamase producing organisms has been compared with the conventional method of inoculation on to agar media. Pharyngeal cultures were obtained from 162 children treated with penicillin for acute tonsillitis. beta-lactamase producing organisms ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.12.1392
更新日期:1984-12-01 00:00:00
abstract::Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203231
更新日期:2015-12-01 00:00:00
abstract:AIMS:Our laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-205732
更新日期:2019-06-01 00:00:00
abstract::Cervical smears from 327 women were examined using the polymerase chain reaction (PCR) targeted to a sequence in the Bam H1 W region of the Epstein-Barr virus (EBV) to determine the prevalence of the virus in the cervix. EBV was detected in 131 (40%) of the 327 women. Of the 235 women with normal cytology, 98 (42%) we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.1.92
更新日期:1994-01-01 00:00:00
abstract::Experiments are described with glass fibre filter paper and complete glass filters as fitted to high pre-vacuum autoclaves to determine whether organisms can grow or penetrate through the filters. A filter consisting of four layers of glass fibre paper fitted inside a sterilizer chamber for a year and used for 96 ster...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.3.259
更新日期:1963-05-01 00:00:00
abstract:AIMS:Cervical cytology biobanking is a feasible concept in cervical pathology and could be an indispensable tool for fundamental and applied molecular biological research. PCR is a powerful molecular technique that can be performed on a variety of cervical sample types including Pap-stained cervical smears. However, si...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.052688
更新日期:2008-05-01 00:00:00
abstract::Fifty-three breast carcinomas were examined by an indirect immunoperoxidase technique for the presence of the alpha-subunit of human chorionic gonadotrophin. Positive staining occurred in 12 (22.6%). There was no correlation between alpha-subunit production and specific histological features, but production was relate...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.3.245
更新日期:1978-03-01 00:00:00
abstract::Phyllodes tumours (PTs) of the breast are true biphasic neoplasms within which interactions between the epithelium and stroma are critical for tumour development and progression. Despite numerous studies reporting the results of ancillary marker investigations in PTs, the current histological grading systems remain un...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2012-201082
更新日期:2013-06-01 00:00:00
abstract::Painful lymphadenopathy developed in eight female patients after massive infusions of iron dextran for sideropaenic anaemia. Fever, arthralgias, and malaise were also features of the clinical picture. The histology of one lymph node is described and changes in it are discussed in relation to the pathogenesis of the sy...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.4.492
更新日期:1968-07-01 00:00:00
abstract:BACKGROUND:Fungal infection is increasingly recognised as an important cause of morbidity and mortality, especially in immunocompromised patients. Little information exists on laboratory services available and the methods used by general microbiology laboratories to diagnose these important infections. AIM:To investig...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2005.034108
更新日期:2006-07-01 00:00:00
abstract:AIM:To compare the sensitivity and staining pattern of the new immunohistochemical antibody to tyrosinase (T311) with S-100, HMB45, and the recently evaluated antibody to melan-A (A103) in a range of melanocytic lesions. METHOD:Archival, formalin fixed, paraffin wax embedded sections from 50 benign and malignant melan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.3.196
更新日期:2001-03-01 00:00:00
abstract::A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.11.1015
更新日期:1977-11-01 00:00:00
abstract:AIMS:The aim of the present study was to immunohistochemically investigate the expression and prognostic significance of putative cancer stem cell markers CD117 (c-kit), CD34, CD20 and CD15 in a cohort of patients with primary choroidal and ciliary body melanoma. METHODS:The immunohistochemical expression of these mar...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203130
更新日期:2016-03-01 00:00:00
abstract::The results of the measurement of vitamin B(12) absorption by counting the radioactivity of 5 ml. serum obtained eight to 10 hours after the ingestion of an oral dose of 0.5 mug. vitamin B(12) labelled with 0.5 muc. (57)Co are compared with those obtained with the urinary excretion (Schilling) test. Inadequate urine c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.6.606
更新日期:1966-11-01 00:00:00
abstract::The mechanism of DNA degradation and its clinical applications were examined. When purified lambda phage and extracted liver DNA were fixed in phosphate buffered formaldehyde, the DNA did not degrade, but there was incomplete digestion with endonuclease. Rat liver tissues were fixed under various conditions and DNA ex...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.9.748
更新日期:1990-09-01 00:00:00
abstract:AIMS:To assess the incidence of nocardia infection over 15 years in a tertiary care hospital. METHODS:Over a 15 year period, Nocardia spp were isolated from 20 patients hospitalised at the Geneva University Hospitals, Switzerland. RESULTS:Sixteen patients had one or more underlying conditions. The median time between...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.016923
更新日期:2004-08-01 00:00:00
abstract:AIMS:We aimed to study epidermal growth factor receptor (EGFR) expression in surgically resected pancreatic ductal adenocarcinomas (PDACs) by immunohistochemistry and their relationship to clinicopathological features, cell proliferation and cell adhesion protein expression. METHODS:A total of 99 PDACs were analysed o...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201662
更新日期:2014-04-01 00:00:00
abstract::A case of cirrhosis of the liver, associated with false-positive serological tests for syphilis and an antibody active against stored red cells, is presented. Methods used in the identification of the ;stored red cell' antibody are described. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.4.460
更新日期:1965-07-01 00:00:00
abstract::An analysis of the plasma proteins in the sol phase of sputum was carried out using quantitative cross immunoelectrophoresis. The average concentrations of nine plasma proteins were estimated in the sol phase of sputum specimens from 30 patients with chronic bronchitis and the values were compared with the concentrati...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.11.852
更新日期:1973-11-01 00:00:00