Abstract:
:Aromatic L-amino acid decarboxylase (AADC) decarboxylates L-DOPA and 5-hydroxytryptophan into dopamine and serotonin, respectively. Starting from a rat AADC cDNA clone isolated in our laboratory, we produced a beta-galactosidase-AADC fusion protein in E. coli. It was purified from inclusion bodies and injected into a rabbit. The antiserum identified AADC on a Western blot of extracts from rat organs as a unique 50 kDa band; it also strongly reacted by immunohistochemistry with dopaminergic and serotonergic neurons. This new beta-galactosidase-AADC fusion protein then constitutes a useful tool for producing AADC as an antigen free of contamination by mammalian proteins.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Krieger M,Tillet Y,Gros F,Thibault Jdoi
10.1016/0304-3940(93)90084-xsubject
Has Abstractpub_date
1993-04-16 00:00:00pages
88-92issue
1eissn
0304-3940issn
1872-7972pii
0304-3940(93)90084-Xjournal_volume
153pub_type
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