Preparation of an antiserum using a fusion protein produced by a cDNA for rat aromatic L-amino acid decarboxylase.

Abstract:

:Aromatic L-amino acid decarboxylase (AADC) decarboxylates L-DOPA and 5-hydroxytryptophan into dopamine and serotonin, respectively. Starting from a rat AADC cDNA clone isolated in our laboratory, we produced a beta-galactosidase-AADC fusion protein in E. coli. It was purified from inclusion bodies and injected into a rabbit. The antiserum identified AADC on a Western blot of extracts from rat organs as a unique 50 kDa band; it also strongly reacted by immunohistochemistry with dopaminergic and serotonergic neurons. This new beta-galactosidase-AADC fusion protein then constitutes a useful tool for producing AADC as an antigen free of contamination by mammalian proteins.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Krieger M,Tillet Y,Gros F,Thibault J

doi

10.1016/0304-3940(93)90084-x

subject

Has Abstract

pub_date

1993-04-16 00:00:00

pages

88-92

issue

1

eissn

0304-3940

issn

1872-7972

pii

0304-3940(93)90084-X

journal_volume

153

pub_type

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