Abstract：
:The sarcomere length (SL) dependence of the calcium sensitivity of force was measured in skinned single twitch and slow-tonic muscle fibres from frog and toad. Twitch and slow-tonic fibres were characterized by location, appearance, physiological response to calcium and by protein band patterns from sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Force-calcium relations were determined for each fibre type at two sarcomere lengths, 2.4 and 3.1 microns. Bathing solution ionic strength (IS) was 200 mM and solution pH was 7.0, 6.0 or 5.5; experiments were also done at IS = 120 mM and pH 7.0. At all pHs and ionic strengths tested, slow-tonic fibres exhibited a slower time course of force development and were more sensitive to calcium than were twitch fibres. Lowering IS increased calcium sensitivity and lowering pH decreased calcium sensitivity in both fibre types. Increasing SL increased the calcium sensitivity of force in both twitch and slow-tonic fibres at pH 7.0 and at both 200 and 120 mM IS. Lowering pH caused a decrease in the length dependence of calcium sensitivity of both fibre types; at pH 5.5 the calcium sensitivity of force in slow-tonic fibres exhibited a slight decrease with increasing SL.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Martyn DA,Coby R,Huntsman LL,Gordon AMdoi
10.1007/BF00132181subject
Has Abstractpub_date
1993-02-01 00:00:00pages
65-75issue
1eissn
0142-4319issn
1573-2657journal_volume
14pub_type
杂志文章abstract：:Two procedures to inhibit Ca(2+) release designed to differentiate between local and common pool mechanisms for the Ca(2+) dependent, fast inactivation of Ca(2+) release in skeletal muscle of the frog were compared. Inhibition by voltage dependent inactivation of Ca(2+) release, without modification of the single chan...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-010-9212-y
更新日期：2010-08-01 00:00:00
abstract：:Our current understanding of the structure of the thin filaments of muscle and the molecular mechanism by which thin filaments regulate muscle contraction are reviewed and discussed. We focus, in particular, on the crucial role played by Jean Hanson in these studies and on later contributions from those whose work she...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章,评审
doi：10.1007/s10974-004-3148-z
更新日期：2004-01-01 00:00:00
abstract：:Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-017-9489-1
更新日期：2017-08-01 00:00:00
abstract：:Calsequestrin type-1 (CASQ1), the main sarcoplasmic reticulum (SR) Ca(2+) binding protein, plays a dual role in skeletal fibers: a) it provides a large pool of rapidly-releasable Ca(2+) during excitation-contraction (EC) coupling; and b) it modulates the activity of ryanodine receptors (RYRs), the SR Ca(2+) release ch...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9277-2
更新日期：2011-12-01 00:00:00
abstract：:Phosphorylation of skeletal myosin regulatory light chain (RLC) occurs in fatigue and may play a role in the inhibition of shortening velocities observed in vivo. Forces and shortening velocities were measured in permeabilized rabbit psoas fibers with either phosphorylated or dephosphorylated RLCs and in the presence ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9162-9
更新日期：2009-01-01 00:00:00
abstract：:Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7-284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosi...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-007-9114-9
更新日期：2007-01-01 00:00:00
abstract：:The optimal utilization of energy substrates in muscle fibers is of primary importance for muscle contraction and whole body physiology. This study aimed to investigate the age-related changes in some indicators of glucose catabolism and fatty acid oxidation in muscles of growing rabbits. Longissimus lumborum (fast-tw...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-2768-7
更新日期：2004-01-01 00:00:00
abstract：:We found that the active tension of C2C12 myotubes that had been subjected to artificial exercise for ~10 days decreased rapidly after termination of the artificial exercise. When differentiated C2C12 myotubes were subjected to continuous 1 Hz artificial exercise for ~10 days, the active tension increased to ~4× compa...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-010-9230-9
更新日期：2010-12-01 00:00:00
abstract：:The properties of spin-labelled myosin, prepared from rabbit skeletal and scallop adductor muscle, on forming a long-lived complex with ADP and vanadate (M.ADP.Vi), have been investigated. In the case of an iodoacetamide-based label attached to rabbit myosin or subfragment 1, M.ADP.Vi formation is characterized by a m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00713154
更新日期：1984-02-01 00:00:00
abstract：:Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-020-09578-3
更新日期：2020-09-01 00:00:00
abstract：:This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a me...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9260-y
更新日期：2011-11-01 00:00:00
abstract：:Hodges and his colleagues identified a 12 amino acid fragment of troponin I (TnI-ip) that inhibits Ca(2+)-activated force and reduces the effectiveness Ca(2+) as an activator. To understand the role of troponin C (TnC) in the extended cooperative interactions of thin filament activation, we compared the effect of TnI-...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-013-9336-y
更新日期：2013-05-01 00:00:00
abstract：:Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-012-9300-2
更新日期：2012-08-01 00:00:00
abstract：:In isolated myosin the reaction sequence of essential thiol groups with N-ethylmaleimide was studied using the following five approaches: kinetics of the modification reaction, effects of modification on enzyme properties, affinity chromatography of isolated subfragment-1 stemming from modified myosin, isolation of cy...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711923
更新日期：1980-03-01 00:00:00
abstract：:Tropomyosins (Tm) in humans are expressed from four distinct genes and by alternate splicing >40 different Tm polypeptide chains can be made. The functional Tm unit is a dimer of two parallel polypeptide chains and these can be assembled from identical (homodimer) or different (heterodimer) polypeptide chains provided...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-013-9353-x
更新日期：2013-08-01 00:00:00
abstract：:Results were obtained from contracting frog muscles by collecting high quality time-resolved, two-dimensional, X-ray diffraction patterns at the British Synchrotron Radiation Source (SERC, Daresbury, Laboratory). The structural transitions associated with isometric tension generation were recorded under conditions in ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00123096
更新日期：1993-06-01 00:00:00
abstract：:The mechanical events following release of ATP from P3-1-(2-nitro)phenylethyladenosine-5'-triphosphate (caged-ATP) in skinned guinea pig taenia coli smooth muscle in rigor were investigated. A rigor force of about 25-35% of the maximal active force was obtained by removing ATP at the plateau of a maximal active contra...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01578427
更新日期：1987-10-01 00:00:00
abstract：:This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depola...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1012289526618
更新日期：2001-01-01 00:00:00
abstract：:The effect of changes in hydrostatic pressure (up to 10 MPa) on the maximally calcium-activated tension in glycerinated rabbit psoas fibres has been examined. The steady active tension was depressed by 0.8% per MPa pressure rise. This pressure sensitivity was enhanced by the pressure of millimolar phosphate and depres...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739967
更新日期：1989-04-01 00:00:00
abstract：:Immunohistochemistry was used to determine the myosin composition of defined fibre types of three embryologically different adult muscles, the oro-facial, masseter and limb muscles. In addition, the myosin composition in whole muscle specimens was analysed with biochemical methods. Both similarities and differences be...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00121158
更新日期：1994-10-01 00:00:00
abstract：:The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00121293
更新日期：1993-08-01 00:00:00
abstract：:The width (D) and the 1,0 lattice spacing (d1,0) at various ionic compositions of mechanically skinned single fibres (from semitendinosus muscle of Rana catesbeiana) were measured at various concentrations of polyvinyl pyrrolidone (PVP K-30, Mn = 40 000) from 0 to 6% at 20 degrees C. In a standard relaxing solution (4...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01753558
更新日期：1986-06-01 00:00:00
abstract：:Scallop S1 has a region sensitive to tryptic hydrolysis not found thus far in S1s of other species, located 65K from the N-terminus as determined by SDS/polyacrylamide-gel electrophoresis. In the presence of actin the S1 heavy chain is preferentially cleaved at this site. The high-salt EDTA and calcium ATPase activiti...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01568891
更新日期：1987-08-01 00:00:00
abstract：:The non-hydrolysable ATP analogue, beta,gamma-imido-adenosine-5'-triphosphate (AMPPNP, adenylilimidodiphosphate) does not reduce the stiffness of demembranated sea urchin sperm flagella measured in the rigor state in the absence of MgATP2- and does not cause relaxation of rigor bends. The competitive inhibitions provi...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712064
更新日期：1981-03-01 00:00:00
abstract：:A popular part of many athletes pre-game regime is to stretch. We examined whether a pre-injury stretching protocol could prevent acute contraction-induced injury. The in situ extensor digitorum longus (EDL) muscle of an anesthetized mouse (80 mg/kg intra-peritoneal) was used. Damage to the muscle from eccentric contr...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1013188001776
更新日期：2001-01-01 00:00:00
abstract：:The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005670507906
更新日期：2000-04-01 00:00:00
abstract：:Cytokinesis is the mechanical process that allows the simplest unit of life, the cell, to divide, propagating itself. To divide, the cell converts chemical energy into mechanical energy to produce force. This process is thought to be active, due in large part to the mechanochemistry of the myosin-II ATPase. The cell's...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1024419510314
更新日期：2002-01-01 00:00:00
abstract：:Gaps in our understanding of muscle mechanics demonstrate that the current model is incomplete. Increasingly, it appears that a role for titin in active muscle contraction might help to fill these gaps. While such a role for titin is increasingly accepted, the underlying molecular mechanisms remain unclear. The goals ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-019-09540-y
更新日期：2020-03-01 00:00:00
abstract：:The ability for muscle to repeatedly generate force is limited by fatigue. The cellular mechanisms behind muscle fatigue are complex and potentially include breakdown at many points along the excitation-contraction pathway. In this paper we construct a mathematical model of the skeletal muscle excitation-contraction p...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-007-9125-6
更新日期：2007-01-01 00:00:00
abstract：:The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005585807179
更新日期：1999-10-01 00:00:00
