Betamethasone activation of CTP:cholinephosphate cytidylyltransferase in vivo is lipid dependent.

Abstract:

:Glucocorticoids increase surfactant phosphatidylcholine synthesis, in part, by stimulating the rate regulatory enzyme CTP:cholinephosphate cytidylyltransferase. This enzyme exists in mammalian lung cytosol as an active lipoprotein form (H-form) and an inactive apoprotein (L-form) species. We administered betamethasone to pregnant rats to examine the mechanisms for glucocorticoid stimulation of cytidylyltransferase activity in fetal lung. The hormone stimulated cytosolic activity threefold, and this effect was nearly abolished after lipid extraction. The addition of lipid extracts isolated from betamethasone-treated cytosolic preparations to L-form species increased enzyme activity to a greater extent than lipid extracts from control lungs. Further, the glucocorticoid increased the proportion of H-form activity from 34 to 55% of the total activity in the fetal lung cytosol. These changes were associated with a marked decrease in the activity of the L-form species. Analysis of the lipid composition of the H-form revealed that betamethasone increased the content of lipid activators, including phosphatidylglycerol and fatty acids. These observations provide evidence that glucocorticoid stimulation of CTP:cholinephosphate cytidylyltransferase in vivo is mediated by a conversion of the inactive form (L-form) to the active species (H-form). These studies further emphasize the critical role of lung lipids in mediating the glucocorticoid activation of this enzyme.

authors

Mallampalli RK,Walter ME,Peterson MW,Hunninghake GW

doi

10.1165/ajrcmb.10.1.8292380

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

48-57

issue

1

eissn

1044-1549

issn

1535-4989

journal_volume

10

pub_type

杂志文章
  • Reversible or irreversible remodeling in pulmonary arterial hypertension.

    abstract::Vascular remodeling is an important pathological feature of pulmonary arterial hypertension (PAH), which leads to increased pulmonary vascular resistance, with marked proliferation of pulmonary artery smooth muscle cells (SMC) and/or endothelial cells (EC). Successful treatment of experimental PAH with a platelet-deri...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章,评审

    doi:10.1165/rcmb.2009-0389TR

    authors: Sakao S,Tatsumi K,Voelkel NF

    更新日期:2010-12-01 00:00:00

  • Endothelin-1 inhibits background two-pore domain channel TASK-1 in primary human pulmonary artery smooth muscle cells.

    abstract::Endothelin (ET)-1 causes long-lasting vasoconstriction and vascular remodeling by interacting with specific G-protein-coupled receptors in pulmonary artery smooth muscle cells (PASMCs), and thus plays an important role in the pathophysiology of pulmonary arterial hypertension. The two-pore domain K(+) channel, TASK-1,...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2008-0412OC

    authors: Tang B,Li Y,Nagaraj C,Morty RE,Gabor S,Stacher E,Voswinckel R,Weissmann N,Leithner K,Olschewski H,Olschewski A

    更新日期:2009-10-01 00:00:00

  • A cystic fibrosis tracheal gland cell line, CF-KM4. Correction by adenovirus-mediated CFTR gene transfer.

    abstract::Human tracheal gland serous (HTGS) cells are now considered one principal pulmonary target for the gene therapy of cystic fibrosis (CF). We developed a CF tracheal gland serous cell line, CF-KM4, obtained by the transformation of primary cultures of CF tracheal gland serous cells homozygous for the DeltaF508 mutation ...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.20.4.3341

    authors: Kammouni W,Moreau B,Becq F,Saleh A,Pavirani A,Figarella C,Merten MD

    更新日期:1999-04-01 00:00:00

  • Role of endogenous cytokines in endotoxin- and interleukin-1-induced pulmonary inflammatory response and oxygen tolerance.

    abstract::Endotoxin lipopolysaccharide and the cytokines, tumor necrosis factor (TNF) and interleukin-1 (IL-1), are known to protect adult rats against O2 toxicity. However, whether the effect of endotoxin is mediated by these cytokines is not clear. We have previously demonstrated that depletion of 84% rat alveolar macrophages...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.12.3.7873200

    authors: Tang G,White JE,Lumb PD,Lawrence DA,Tsan MF

    更新日期:1995-03-01 00:00:00

  • Peroxisome Proliferator-Activated Receptor γ and microRNA 98 in Hypoxia-Induced Endothelin-1 Signaling.

    abstract::Endothelin-1 (ET-1) plays a critical role in endothelial dysfunction and contributes to the pathogenesis of pulmonary hypertension (PH). We hypothesized that peroxisome proliferator-activated receptor γ (PPARγ) stimulates microRNAs that inhibit ET-1 and pulmonary artery endothelial cell (PAEC) proliferation. The objec...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2014-0337OC

    authors: Kang BY,Park KK,Kleinhenz JM,Murphy TC,Green DE,Bijli KM,Yeligar SM,Carthan KA,Searles CD,Sutliff RL,Hart CM

    更新日期:2016-01-01 00:00:00

  • p42/44 Mitogen-activated protein kinase regulated by p53 and nitric oxide in human pulmonary arterial smooth muscle cells.

    abstract::Although nitric oxide (NO) is known to inhibit vascular smooth muscle cell proliferation, the subcellular molecular mechanisms involved with the inhibitory signal transduction pathways are uncertain. We investigated the effect of exogenous NO on cell proliferation and the expression of p53, p21, and phosphorylated p42...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2003-0397OC

    authors: Mizuno S,Kadowaki M,Demura Y,Ameshima S,Miyamori I,Ishizaki T

    更新日期:2004-08-01 00:00:00

  • Upregulation of interleukin-4 receptor by interferon-gamma: enhanced interleukin-4-induced eotaxin-3 production in airway epithelium.

    abstract::Airway epithelial cells produce a number of chemokines, including eotaxins. Among the three known eotaxins, T helper (Th) type 2 cytokines have been observed to induce the expression of eotaxin-3 mRNA. This study investigated the effect of interferon (IFN)-gamma, a Th1 cytokine, on Th2 cytokine-induced eotaxin-3 produ...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2004-0128OC

    authors: Yamamoto S,Kobayashi I,Tsuji K,Nishi N,Muro E,Miyazaki M,Zaitsu M,Inada S,Ichimaru T,Hamasaki Y

    更新日期:2004-10-01 00:00:00

  • Monocyte-macrophage differentiation induced by human upper airway epithelial cells.

    abstract::We examined the ability of conditioned medium (CM) generated by human upper airway epithelial (Ep) cells from normal (NN) and inflamed, allergic rhinitis (AR) and nasal polyp (NP) tissues to induce monocytic differentiation of hemopoietic progenitors of the HL-60 myeloid leukemia cell line in vitro. In HL-60 cells cul...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb/4.3.255

    authors: Ohtoshi T,Vancheri C,Cox G,Gauldie J,Dolovich J,Denburg JA,Jordana M

    更新日期:1991-03-01 00:00:00

  • Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils.

    abstract::Eosinophils are an important source of leukotriene (LT)C(4), which can be synthesized within lipid bodies-cytoplasmic organelles where eicosanoid formation may take place. Allergy-driven lipid body formation and function have never been investigated. Here, we studied the in vivo induction and role of lipid bodies with...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2005-0145OC

    authors: Vieira-de-Abreu A,Assis EF,Gomes GS,Castro-Faria-Neto HC,Weller PF,Bandeira-Melo C,Bozza PT

    更新日期:2005-09-01 00:00:00

  • Neutrophil-Expressed p21/waf1 Favors Inflammation Resolution in Pseudomonas aeruginosa Infection.

    abstract::Neutrophil-associated inflammation during Pseudomonas aeruginosa lung infection is a determinant of morbidity in cystic fibrosis (CF). Neutrophil apoptosis is a key factor in inflammation resolution and is controlled by cytosolic proliferating cell nuclear antigen (PCNA). p21/Waf1, a cyclin-dependent kinase inhibitor,...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2015-0047OC

    authors: Martin C,Ohayon D,Alkan M,Mocek J,Pederzoli-Ribeil M,Candalh C,Thevenot G,Millet A,Tamassia N,Cassatella MA,Thieblemont N,Burgel PR,Witko-Sarsat V

    更新日期:2016-05-01 00:00:00

  • Elevated levels of the IGF-binding protein protease MMP-1 in asthmatic airway smooth muscle.

    abstract::We have previously demonstrated that the asthma-associated proinflammatory eicosanoid leukotriene D4 (LTD4) is co-mitogenic with insulin-like growth factors (IGFs) in airway smooth-muscle (ASM) cells in vitro. This synergistic effect of LTD4 and IGF on ASM cell growth involves proteolysis of ASM-produced IGF binding p...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.20.2.3148

    authors: Rajah R,Nachajon RV,Collins MH,Hakonarson H,Grunstein MM,Cohen P

    更新日期:1999-02-01 00:00:00

  • Acute allergen-induced airway remodeling in atopic asthma.

    abstract::Studies in animals and in human atopic skin suggest that allergen challenge may activate acute tissue remodeling changes via transforming growth factor-beta pathways. We determined whether inhalational allergen challenge in subjects with mild asthma induces similar acute changes to the airway epithelial mesenchymal tr...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 临床试验,杂志文章

    doi:10.1165/rcmb.2004-0193OC

    authors: Phipps S,Benyahia F,Ou TT,Barkans J,Robinson DS,Kay AB

    更新日期:2004-12-01 00:00:00

  • Calcitriol and its synthetic analogue MC 903 inhibit the interleukin-2-induced migration of human lymphocytes.

    abstract::Sarcoidosis is characterized by the accumulation of activated lymphocytes in the lungs and other organs and by the spontaneous production of the active form of vitamin D, calcitriol. We hypothesized that calcitriol may modulate the responsiveness of human lymphocytes to the relevant biologic mediator, interleukin-2 (I...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.12.6.7766430

    authors: Fraher LJ,Caveney AN,McFadden RG

    更新日期:1995-06-01 00:00:00

  • Proteinase-activated receptor-2 and human lung epithelial cells: disarming by neutrophil serine proteinases.

    abstract::Proteinase-activated receptor (PAR)-2 is cleaved within its aminoterminal extracellular domain by serine proteinases such as trypsin, unmasking a new aminoterminus starting with the sequence SLIGKV, which binds intramolecularly and activates the receptor. PAR-2 has been reported to be involved in inflammation within t...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.4908

    authors: Dulon S,Candé C,Bunnett NW,Hollenberg MD,Chignard M,Pidard D

    更新日期:2003-03-01 00:00:00

  • ST depression, arrhythmia, vagal dominance, and reduced cardiac micro-RNA in particulate-exposed rats.

    abstract::Recently, investigators demonstrated associations between fine particulate matter (PM)-associated metals and adverse health effects. Residual oil fly ash (ROFA), a waste product of fossil fuel combustion from boilers, is rich in the transition metals Fe, Ni, and V, and when released as a fugitive particle, is an impor...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2009-0456OC

    authors: Farraj AK,Hazari MS,Haykal-Coates N,Lamb C,Winsett DW,Ge Y,Ledbetter AD,Carll AP,Bruno M,Ghio A,Costa DL

    更新日期:2011-02-01 00:00:00

  • Cyclic stretch-induced oxidative stress increases pulmonary alveolar epithelial permeability.

    abstract::Mechanical ventilation with high tidal volumes has been associated with pulmonary alveolar flooding. Understanding the mechanisms underlying cyclic stretch-induced increases in alveolar epithelial permeability may be important in designing preventive measures for acute lung injury. In this work, we assessed whether cy...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2012-0252OC

    authors: Davidovich N,DiPaolo BC,Lawrence GG,Chhour P,Yehya N,Margulies SS

    更新日期:2013-07-01 00:00:00

  • IL-5 induces suspended eosinophils to undergo unique global reorganization associated with priming.

    abstract::The experiments described herein define a unique program of polarization of suspended human eosinophils stimulated with IL-5 family cytokines. We found that eosinophil granules and the nucleus move in opposite directions to form, respectively, a granular compartment and the nucleopod, a specialized uropod occupied by ...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2013-0181OC

    authors: Han ST,Mosher DF

    更新日期:2014-03-01 00:00:00

  • Inhibition of pulmonary artery smooth muscle cell growth by hypoxanthine, xanthine, and uric acid.

    abstract::We have previously reported that medium conditioned by hypoxic pulmonary artery endothelial cells (ECCM) contains a factor of small molecular weight that inhibits the growth of pulmonary artery smooth muscle cells (SMC). We postulated that this factor might be a breakdown product of ATP and, therefore, measured the le...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb/6.6.617

    authors: Hassoun PM,Shedd AL,Lanzillo JJ,Thappa V,Landman MJ,Fanburg BL

    更新日期:1992-06-01 00:00:00

  • Potential role of soluble TRAIL in epithelial injury in children with severe RSV infection.

    abstract::Lower respiratory tract infection by respiratory syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies in adults and animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate lung injury by causing apoptosis of epithelial cells. The main goal of the ...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2009-0100OC

    authors: Bem RA,Bos AP,Wösten-van Asperen RM,Bruijn M,Lutter R,Sprick MR,van Woensel JB

    更新日期:2010-06-01 00:00:00

  • Critical role of serum response factor in pulmonary myofibroblast differentiation induced by TGF-beta.

    abstract::Transforming growth factor-beta (TGF-beta) is a cytokine implicated in wound healing and in the pathogenesis of pulmonary fibrosis. TGF-beta stimulates myofibroblast differentiation characterized by expression of contractile smooth muscle (SM)-specific proteins such as SM-alpha-actin. In the present study, we examined...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2008-0288OC

    authors: Sandbo N,Kregel S,Taurin S,Bhorade S,Dulin NO

    更新日期:2009-09-01 00:00:00

  • Vitronectin and fibronectin function as glucan binding proteins augmenting macrophage responses to Pneumocystis carinii.

    abstract::beta-glucans represent major structural components of fungal cell walls. We recently reported that Pneumocystis carinii beta-glucans stimulate alveolar macrophages to release proinflammatory cytokines. Macrophage activation by beta-glucan is augmented by serum, implying the presence of circulating factors that interac...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.25.2.4427

    authors: Vassallo R,Kottom TJ,Standing JE,Limper AH

    更新日期:2001-08-01 00:00:00

  • Lung-specific loss of α3 laminin worsens bleomycin-induced pulmonary fibrosis.

    abstract::Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2014-0057OC

    authors: Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

    更新日期:2015-04-01 00:00:00

  • Regulation of hydrogen peroxide generation in cultured endothelial cells.

    abstract::Endogenous hydrogen peroxide (H2O2) release from aortic endothelial cells was studied in the presence of antioxidant enzyme inhibitors, mitochondrial inhibitors, a microsomal cytochrome P-450 inhibitor, and after oxidative stress induced with H2O2 or menadione. Extracellular H2O2 generation was determined spectrofluor...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb/6.2.175

    authors: Kinnula VL,Whorton AR,Chang LY,Crapo JD

    更新日期:1992-02-01 00:00:00

  • Pulmonary macrophage subpopulations in the induction and resolution of acute lung injury.

    abstract::Macrophages are key orchestrators of the inflammatory and repair responses in the lung, and the diversity of their function is indicated by their polarized states and distinct subpopulations and localization in the lung. Here, we characterized the pulmonary macrophage populations in the interstitial and alveolar compa...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2012-0090OC

    authors: Johnston LK,Rims CR,Gill SE,McGuire JK,Manicone AM

    更新日期:2012-10-01 00:00:00

  • Eicosanoid production in rabbit tracheal epithelium by adenine nucleotides: mediation by P2-purinoceptors.

    abstract::Adenosine triphosphate (ATP) acting through epithelial nucleotide receptors exerts multiple physiologic actions on airway mucociliary clearance and caliber. However, the effect of ATP on arachidonate metabolism in the airway remains unknown. In this study, the ability of ATP to regulate eicosanoid production was studi...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb.13.4.7546770

    authors: Aksoy MO,Borenstein M,Li XX,Kelsen SG

    更新日期:1995-10-01 00:00:00

  • Tuberculosis susceptibility of diabetic mice.

    abstract::Increased susceptibility to infections, including tuberculosis (TB), is a major cause of morbidity and mortality in patients with diabetes. Despite the clinical importance of this problem, little is known about how diabetes impairs protective immunity. We modeled this phenomenon by infecting acute (< or = 1 mo) or chr...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2006-0478OC

    authors: Martens GW,Arikan MC,Lee J,Ren F,Greiner D,Kornfeld H

    更新日期:2007-11-01 00:00:00

  • NOX1 Promotes Mesothelial-Mesenchymal Transition Through Modulation of ROS-mediated Signaling.

    abstract::Pleural organization may occur after empyema or complicated parapneumonic effusion and can result in restrictive lung disease with pleural fibrosis (PF). Pleural mesothelial cells; PMCs, may contribute to PF through acquisition of a profibrotic phenotype; meso-mesenchymal transition (MesoMT), which is characterized by...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2020-0077OC

    authors: Qin W,Jeffers A,Owens S,Chauhan P,Komatsu S,Qian G,Guo X,Ikebe M,Idell S,Tucker TA

    更新日期:2021-01-29 00:00:00

  • Role of tyrosine kinases in IgE-mediated signal transduction in human lung mast cells and basophils.

    abstract::Recent evidence suggests that tyrosine kinases play an important role in signal transduction mechanisms utilized by a range of different agonists in many cell types. We have investigated the effects of four different inhibitors of tyrosine kinases on IgE-dependent histamine release from human lung mast cells and basop...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/ajrcmb/7.6.637

    authors: Lavens SE,Peachell PT,Warner JA

    更新日期:1992-12-01 00:00:00

  • Long-Term Microevolution of Pseudomonas aeruginosa Differs between Mildly and Severely Affected Cystic Fibrosis Lungs.

    abstract::Chronic airway infections with Pseudomonas aeruginosa determine morbidity in most individuals with cystic fibrosis (CF). P. aeruginosa may persist for decades in CF lungs, which provides a rare opportunity to study the long-term within-host evolution of a bacterial airway pathogen. In this work, we sought to resolve t...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2017-0356OC

    authors: Klockgether J,Cramer N,Fischer S,Wiehlmann L,Tümmler B

    更新日期:2018-08-01 00:00:00

  • Human rhinovirus-induced epithelial production of CXCL10 is dependent upon IFN regulatory factor-1.

    abstract::Human rhinovirus (HRV) infections are associated with exacerbations of lower-airway diseases. HRV-induced production of proinflammatory chemokines, such as CXCL10, from infected airway epithelial cells may play a role in the pathogenesis of exacerbations. We have previously shown that the MAP/ERK kinase (MEK) pathway ...

    journal_title:American journal of respiratory cell and molecular biology

    pub_type: 杂志文章

    doi:10.1165/rcmb.2009-0203OC

    authors: Zaheer RS,Proud D

    更新日期:2010-10-01 00:00:00