Abstract:
STUDY DESIGN:This was an experimental study to compare various methods of storing rib autograft between stages of spinal surgery. OBJECTIVE:The authors' goal was to determine the optimum conditions for storing bone graft, while preserving its osteogenic potential. SUMMARY OF BACKGROUND DATA:It never has been demonstrated that osteoblastic function can be preserved after bone graft is stored. Other researchers have shown preservation of cellular function, but not its nature. METHODS:Excised rib was stored in one of several ways: in culture medium at -196 C, 4 C, or 37 C, or in saline at 4 C for 2 weeks and then cultured at 37 C. Any cells produced were characterized using standard techniques. RESULTS:Bone stored at 37 C and -196 C retained its osteogenic potential. Bone stored at 4 C in saline did not. Bone stored at 4 C in culture medium was intermediate in its preservation of osteogenic capacity. CONCLUSIONS:Bone stored under suitable conditions retains osteogenic potential, and when reimplanted will add osteogenic cells to the fusion mass.
journal_name
Spine (Phila Pa 1976)journal_title
Spineauthors
Evans CE,Williamson JBdoi
10.1097/00007632-199404000-00004subject
Has Abstractpub_date
1994-04-01 00:00:00pages
752-4issue
7eissn
0362-2436issn
1528-1159journal_volume
19pub_type
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