Abstract:
:Cytokines such as tumor necrosis factor alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 8 (IL-8), IL-6, IL-1 alpha, and IL-1 beta produced during the immune and inflammatory responses to bacterial stimuli have been reported to interact with polymorphonuclear neutrophil (PMN) activities. However, contradictory findings on their direct and priming effects on the PMN oxidative burst, which is essential for bacterial killing, have been reported. We have used a flow cytometry method to study the effects of these cytokines on the oxidative burst of PMN in whole blood to avoid PMN activation related to isolation procedures. None of the cytokines tested directly activated the PMN oxidative burst, but they did have differential priming effects on the oxidative burst in response to bacterial N-formyl peptides. TNF, GM-CSF, and IL-8 strongly primed a subpopulation of PMN to produce H2O2 in response to N-formyl-methionyl-leucyl-phenylalanine (FMLP), while IL-1 alpha, IL-1 beta, and IL-6 failed to do so. Furthermore, the addition of TNF, GM-CSF, or IL-8 to whole blood increased the capacity of a subpopulation of PMN to bind N-formyl peptides, a phenomenon that could account, at least in part, for the strong H2O2 production in response to FMLP after priming by the cytokines. The size of the primed hyperresponsive subpopulation was greater after priming with TNF or GM-CSF than after priming with IL-8. However, GM-CSF, TNF, and IL-8 at suboptimal concentrations cooperated in the induction of a subpopulation hyperresponsive to FMLP. These results show that, of the various proinflammatory cytokines tested, TNF, GM-CSF, and IL-8 strongly prime the PMN oxidative burst in response to bacterial peptides in whole blood and suggest that these cytokines may play a critical role in bacterial killing in vivo.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Elbim C,Bailly S,Chollet-Martin S,Hakim J,Gougerot-Pocidalo MAdoi
10.1128/IAI.62.6.2195-2201.1994subject
Has Abstractpub_date
1994-06-01 00:00:00pages
2195-201issue
6eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
abstract::The addition of inhibitors of cell wall biosynthesis to exponential-phase cultures of Streptococcus mutans may do one of three things, depending on the concentrations used: (i) prevent cell division at a time coincident with the onset of chromosome replication, (ii) prevent cell division later in the cell cycle coinci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.28-32.1983
更新日期:1983-07-01 00:00:00
abstract::Bordetella dermonecrotizing toxin (DNT) stimulates the assembly of actin stress fibers and focal adhesions by deamidating Gln63 of the small GTPase Rho. To clarify the functional and structural organization of DNT, we cloned and sequenced the DNT gene and examined the functions of various DNT mutants. Our analyses of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3727-3732.1999
更新日期:1999-08-01 00:00:00
abstract::In vivo expression of pili by Escherichia coli in the urine of 41 adults with lower urinary tract infections was analyzed by immunostaining with polyclonal antiserum to type 1 and P pili. Type 1 pili were detected in 31 of 41 urine specimens, while P pili were detected in 6 of 18 specimens. The piliation status of bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1656-1662.1989
更新日期:1989-06-01 00:00:00
abstract::After interaction with tracheal epithelial cells, Bordetella pertussis induces the secretion of interleukin-6. This secretion is dependent on the expression of adenylate cyclase-hemolysin by the bacterium but not on the expression of other characterized bacterial toxins or adhesins. This finding confirms the important...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5530-5533.2004
更新日期:2004-09-01 00:00:00
abstract::No studies to date clearly define the interactions between Porphyromonas gingivalis and human peripheral blood polymorphonuclear leukocytes (PMN), nor has a protective role for antibody to P. gingivalis been defined. Using a fluorochrome phagocytosis microassay, we investigated PMN phagocytosis and killing of P. gingi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2097-2104.1991
更新日期:1991-06-01 00:00:00
abstract::The triggering receptor expressed on myeloid cell type 1 (TREM-1) is a cell surface molecule that has been identified on both human and murine polymorphonuclear neutrophils and mature monocytes. The activation of TREM-1 in the presence of microbial components amplifies the inflammatory response and may be responsible ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2823-2830.2006
更新日期:2006-05-01 00:00:00
abstract::Female mice infected at birth with 10(7) 50% tissue culture infective doses of polyoma virus were mated when at least 6 weeks old. Polyoma was not detected in any tissues of 27 female mice before mating except for trace amounts in the kidneys of 2 mice, but late in gestation polyoma virus could be found in the kidneys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.998-1002.1979
更新日期:1979-09-01 00:00:00
abstract::Several strains of gram-negative bacteria (seven genera, eight species) isolated from patients with urinary tract infections were found to hydrolyze myeloma immunoglobulin A (IgA) protein. Human IgG and IgM and colostrum IgA were not degraded by these organisms. Examination of cleavage digests showed two fragments of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.11-13.1984
更新日期:1984-01-01 00:00:00
abstract::We have developed an improved scheme for the classification of environmental and clinical isolates of Histoplasma capsulatum that is based on analysis of mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA). Strains were initially divided into mtDNA groups according to restriction digests of whole-cell DNA and Southern ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1409-1412.1989
更新日期:1989-05-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHI) is an important etiological agent of otitis media (OM) and of exacerbated chronic obstructive pulmonary diseases (COPD). Inflammation is a hallmark of both diseases. Interleukin-8 (IL-8), one of the important inflammatory mediators, is induced by NTHI and may play a significan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5523-5530.2003
更新日期:2003-10-01 00:00:00
abstract::Trypanosoma cruzi species is categorized into six discrete typing units (TcI to TcVI) of which TcI is most abundantly noted in the sylvatic transmission cycle and considered the major cause of human disease. In our study, the TcI strains Colombiana (COL), SylvioX10/4 (SYL), and a cultured clone (TCC) exhibited differe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00193-16
更新日期:2016-05-24 00:00:00
abstract::Neisseria meningitidis is a major cause of sepsis and meningitis but is also a common commensal, present in the nasopharynx of between 8 and 20% of healthy individuals. During carriage, the bacterium is found on the surface of the nasopharyngeal epithelium and in deeper tissues, while to develop disease the meningococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01377-09
更新日期:2010-09-01 00:00:00
abstract::Mannheimia haemolytica, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this proces...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00312-08
更新日期:2009-01-01 00:00:00
abstract::This study was undertaken to determine the sequence of events in the microenvironment of the intestinal tract that culminate in the symptoms of cholera and to attempt to define more clearly the mechanisms involved in antibacterial immunity. The extent to which mucus occurs in the normal intestine of rabbits and the ap...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.195-203.1976
更新日期:1976-01-01 00:00:00
abstract::Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02790-14
更新日期:2015-04-01 00:00:00
abstract::Rickettsia prowazeki, pretreated with typhus immune human serum, readily infects, and grows in, chicken embryo cells in culture. This finding is similar to those of previous studies which showed that typhus rickettsiae, pretreated with immune serum, grow in cells of the yolk sac of embryonated hen eggs and in the cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.571-575.1974
更新日期:1974-03-01 00:00:00
abstract::Pneumolysin, the cholesterol-dependent cytolysin of Streptococcus pneumoniae, induces inflammatory and apoptotic events in mammalian cells. Toll-like receptor 4 (TLR4) confers resistance to pneumococcal infection via its interaction with pneumolysin, but the underlying mechanisms remain to be identified. In the presen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6479-6487.2005
更新日期:2005-10-01 00:00:00
abstract::Mutant cultures that lacked the ability to produce filamentous hemagglutinin were obtained by UV irradiation of Bordetella pertussis strain 18-323. Various biological activities of these mutants were compared with those of the parent culture. It was found that the leukocytosis-promoting, histamine-sensitizing, and mou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1018-1023.1982
更新日期:1982-03-01 00:00:00
abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.153-158.1985
更新日期:1985-04-01 00:00:00
abstract::Three dominant proteins (41, 48, and 49 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in purified preparations of the extracellular aspartyl proteinase (AP) of Candida albicans. All three proteins bound to the specific carboxyl proteinase ligand, pepstatin A, and were assoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2030-2036.1993
更新日期:1993-05-01 00:00:00
abstract::In this study we tested the in vitro and in vivo anti-Cryptococcus neoformans activity of an antilaminarin (anti-beta-glucan) monoclonal antibody (MAb 2G8) (immunoglobulin G2b) which was previously shown to inhibit the growth of beta-glucan-exposing Candida albicans cells. Here we show that MAb 2G8 binds to the cell w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00278-07
更新日期:2007-11-01 00:00:00
abstract::Gamma interferon (IFN-γ) drives antiparasite responses and immunopathology during infection with Plasmodium species. Immunity-related GTPases (IRGs) are a class of IFN-γ-dependent proteins that are essential for cell autonomous immunity to numerous intracellular pathogens. However, it is currently unknown whether IRGs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02701-14
更新日期:2015-04-01 00:00:00
abstract::Intravenous injection of rat anti-mouse gamma interferon (IFN-gamma) monoclonal antibody as well as rabbit anti-mouse tumor necrosis factor (TNF) antibody into mice which had received a sublethal infection with Listeria monocytogenes cells resulted in acceleration of listeriosis. Endogenous IFN-gamma seemed to be prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3331-3337.1989
更新日期:1989-11-01 00:00:00
abstract::Plasmodium falciparum's ability to invade erythrocytes is essential for its survival within the human host. Immune mechanisms that impair this ability are therefore expected to contribute to immunity against the parasite. Plasma of humans who are naturally exposed to malaria has been shown to have growth-inhibitory ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06190-11
更新日期:2012-05-01 00:00:00
abstract::Fibronectin binds to Streptococcus pyogenes, and this binding is inhibited by lipoteichoic acid (LTA). Previous studies have shown that LTA can be released from S. pyogenes by treatment with penicillin. Penicillin released LTA from both S. pyogenes and Staphylococcus aureus; however, the binding of fibronectin correla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.529-535.1986
更新日期:1986-02-01 00:00:00
abstract::Staphylococcus aureus and Salmonella enterica serovar Dublin invade osteoblasts and are causative agents of human bone disease. In the present study, we examined the ability of S. aureus and Salmonella serovar Dublin to induce the production of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by normal ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1581-1586.2001
更新日期:2001-03-01 00:00:00
abstract::The mechanisms that govern the initial interaction between Paracoccidioides brasiliensis, a primary dimorphic fungal pathogen, and cells of the innate immunity need to be clarified. Our previous studies showed that Toll-like receptor 2 (TLR2) and TLR4 regulate the initial interaction of fungal cells with macrophages a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00375-10
更新日期:2011-06-01 00:00:00
abstract::Clostridium difficile toxins A and B (TcdA and TcdB) are the causative agents of antibiotic-associated pseudomembranous colitis. Mucosal mast cells play a crucial role in the inflammatory processes underlying this disease. We studied the direct effects of TcdA and TcdB on the human mast cell line HMC-1 with respect to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00195-07
更新日期:2007-08-01 00:00:00
abstract::The lipopolysaccharide (LPS)-potentiating effect of serum is due to LPS-binding protein (LBP), which facilitates the binding of LPS to CD14 receptors. We observed a remarkable heat sensitivity of recombinant LBP and various sera with respect to both immunoreactivity (measured by enzyme-linked immunosorbent assay) and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.363-365.1995
更新日期:1995-01-01 00:00:00