Abstract:
:Escherichia coli and many other microorganisms synthesize aromatic amino acids through the condensation reaction between phosphoenolpyruvate (PEP) and erythrose 4-phosphate to form 3-deoxy-D-arabinoheptulosonate 7-phosphate (DAHP). It has been shown that overexpression of transketolase increases the production of DAHP in an aroB mutant strain (unable to further metabolize DAHP) with elevated DAHP synthase. However, the yield (percent conversion) of DAHP from glucose is still low. Stoichiometric analysis shows that many enzymes compete for intracellular PEP. In particular, the phosphotransferase system, responsible for glucose transport in E. coli, uses PEP as a phosphate donor and converts it to pyruvate, which is less likely to recycle back to PEP. This stoichiometric limitation greatly reduces the yield of aromatic metabolites. To relieve this limitation, we overexpressed PEP synthase in the presence of glucose and showed that it increased the final concentration and the yield of DAHP by almost twofold, to a near theoretical maximum. The PEP synthase effect is not observed without overproduced transketolase, suggesting that erythrose 4-phosphate is the first limiting metabolite. This result demonstrates the utility of pathway analysis and the limitation of central metabolites in the high-level overproduction of desired metabolites.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Patnaik R,Liao JCdoi
10.1128/AEM.60.11.3903-3908.1994subject
Has Abstractpub_date
1994-11-01 00:00:00pages
3903-8issue
11eissn
0099-2240issn
1098-5336journal_volume
60pub_type
杂志文章abstract::The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...
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doi:10.1128/AEM.02349-10
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pub_type: 杂志文章
doi:10.1128/aem.68.10.5167-5169.2002
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1983-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00