Abstract：
AIMS:To assess the consistency and reproducibility of assessment of c-erbB-2 immunostaining, and to examine some of the problems relating to inter- and intraobserver variability in the documentation of positive staining; to profile the spectrum of cytoplasmic and membranous staining in a wide range of tumour types. METHODS:A total of 283 neoplasms were examined for immunohistochemical expression of the c-erbB-2 oncoprotein. Three independent observers were required to assess intensity both of membrane and cytoplasmic staining on a three point and then a four point scale. Extent of positive staining was also assessed on a two point scale. A minimum of two weeks elapsed between assessments using the differing scales. RESULTS:Positive membrane staining was documented by one or more observers in 16.6% of tumours examined. This positivity was largely restricted to bladder, renal, and breast tumours. The overall level of disagreement as to the presence or absence of membranous staining was 11.3%. Cytoplasmic staining was identified in 55.5% of tumours studied. The level of disagreement as to the presence or absence of cytoplasmic staining was 26.5%. CONCLUSIONS:Intraobserver variability was minimal, indicating that each pathologist was adhering to internal reproducible standards. Interobserver variability was greater, indicating that the interpretation of c-erbB-2 immunostaining may require set guidelines. It is suggested that assessment should be referenced to a standard positive control, that a three tier system for grading of intensity and a two tier system for grading of extent should be adopted, and that the evaluation should be agreed by at least two pathologists. The presence of cytoplasmic staining should continue to be routinely recorded until its biological role and clinical implications are fully understood.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Kay EW,Walsh CJ,Cassidy M,Curran B,Leader Mdoi
10.1136/jcp.47.9.816subject
Has Abstractpub_date
1994-09-01 00:00:00pages
816-22issue
9eissn
0021-9746issn
1472-4146journal_volume
47pub_type
杂志文章abstract：CLINICAL QUESTION:A 45-year-old woman presented with painless vaginal bleeding for 2 months. A biopsy was taken from the uterine cervix, and poorly differentiated adenocarcinoma was suspected. After three cycles of neoadjuvant chemotherapy treatment, the patient underwent radical hysterectomy with bilateral salpingo-oo...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2018-205081
更新日期：2018-11-01 00:00:00
abstract：AIMS:The signalling system of phosphoinositides (PIs) is involved in a number of cell and tissue functions including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation and cell and tissue polarity. Recently, a role in cell migration was hypothesised for PI and related molecules including...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2011-200096
更新日期：2011-10-01 00:00:00
abstract：AIM:To assess the clinical value of malignancy grading systems compared with nuclear DNA content, protein p53, and angiogenesis for predicting recurrence of stage I (UICC, 1987) tongue carcinomas. METHODS:Histopathological malignancy grading according to Jakobsson and tumour front grading according to Bryne et al were...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.1.35
更新日期：1999-01-01 00:00:00
abstract：:A "Centrifichem" centrifugal analyser was found using a double fluorescence/bacterial tracer method to be a source of airborne and surface contamination in the laboratory. The airborne contamination was controlled by a modification incorporating a filter. Ubiquitous surface contamination generally accompanied the proc...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.41.11.1229
更新日期：1988-11-01 00:00:00
abstract：:Synovial sarcoma is a distinctive soft tissue neoplasm with monophasic and biphasic forms. It is typically a deep-seated soft tissue tumour of the extremities of young adults and occasional cases have been described in large peripheral nerves. A rare example has a predominance of the glandular component and may mimic ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2007.053124
更新日期：2008-05-01 00:00:00
abstract：:Mixed lymphocyte reactions (MLRs) were measured in 25 HLA-A, B and DR compatible sibling bone marrow transplants. Only four of 25 MLRs were positive and in these the low reactivity was of doubtful clinical importance. There was no correlation between MLR and the subsequent development or severity of graft versus host ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.41.11.1155
更新日期：1988-11-01 00:00:00
abstract：:Enteroviruses are rare causes of acute focal encephalitis. A fatal case of echovirus type 9 infection is reported in a 9 month old boy who presented with a fever and a macular rash followed by two focal seizures. Echovirus type 9 was isolated from lung tissue after seven days. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.9.865
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND:Serum prostate specific antigen (PSA) increases after radical prostatectomy are thought to indicate recurrent disease, although some suggest they result from benign prostatic epithelial tissue left at surgical margins. AIMS:To investigate whether presence, location, and extent of benign prostatic tissue at ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2004.024182
更新日期：2005-07-01 00:00:00
abstract：:A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale gre...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.35.6.642
更新日期：1982-06-01 00:00:00
abstract：:The aggregating effects of adenosine diphosphate, thrombin, 5-hydroxytryptamine, tryptamine, adrenaline and noradrenaline, and tri-ethyl tin have been carefully compared. The first three compounds in some circumstances produce remarkably similar effects although there are important differences. The kinetics of aggrega...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.17.3.275
更新日期：1964-05-01 00:00:00
abstract：AIMS:To elucidate potential mechanisms of drug resistance, levels of topoisomerase II alpha mRNA, a target for cytostatic drugs, were measured in cryopreserved tumour tissue from 36 patients with non-Hodgkin's lymphoma. To evaluate the potential association between topoisomerase II alpha and cell proliferation, Ki-67 i...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.50.1.22
更新日期：1997-01-01 00:00:00
abstract：:Fifty-three breast carcinomas were examined by an indirect immunoperoxidase technique for the presence of the alpha-subunit of human chorionic gonadotrophin. Positive staining occurred in 12 (22.6%). There was no correlation between alpha-subunit production and specific histological features, but production was relate...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.3.245
更新日期：1978-03-01 00:00:00
abstract：:A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinoma...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.43.3.213
更新日期：1990-03-01 00:00:00
abstract：AIMS:Accurate assessment of HER2 status is essential for selection of patients for HER2-targeted treatment such as trastuzumab. This study investigated the hypothesis that delayed fixation impairs HER2 assessment. METHODS:9 carcinomas were received fresh, and samples were fixed immediately or put in fixative at time i...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2013-201978
更新日期：2014-07-01 00:00:00
abstract：:Patients with congenital lung cysts are at increased risk of developing carcinoma, but the mechanisms concerned are not clear. The case of a young adult who developed a bronchioloalveolar carcinoma associated with a cystic congenital adenomatoid malformation is reported. The adjacent lung showed an unusual intra-alveo...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.40.6.612
更新日期：1987-06-01 00:00:00
abstract：BACKGROUND:A genetic component to the development of chronic fatigue syndrome (CFS) has been proposed, and a possible association between human leucocyte antigen (HLA) class II antigens and chronic fatigue immune dysfunction has been shown in some, but not all, studies. AIMS:To investigate the role of HLA class II ant...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2004.022681
更新日期：2005-08-01 00:00:00
abstract：:The principal neuropathological and general pathological findings in a group of 24 patients with renal transplants who died in a nine-year period at the Western Infirmary, Glasgow, are described. Opportunistic infections--bacterial, protozoal, and fungal--were the commonest causes of death. Other causes included cardi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.35.1.31
更新日期：1982-01-01 00:00:00
abstract：AIMS:To develop a rapid, sensitive, and safe method for the analysis of von Willebrand factor (vWf) multimers in plasma or platelet lysates. METHOD:Analysis of vWf multimers was carried out by sodium dodecyl sulphate-agarose discontinuous gel electrophoresis followed by protein transfer to nitrocellulose membranes by ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.5.470
更新日期：1993-05-01 00:00:00
abstract：:A 35 year old man with a fatal Campylobacter jejuni infection is described. He had HbE/beta zero thalassaemia and had undergone splenectomy nine months previously for hypersplenism; he also had chronic hepatitis C infection. He presented with high grade fever but no gastrointestinal symptoms and rapidly progressed to ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.50.5.436
更新日期：1997-05-01 00:00:00
abstract：:Three adults with alpha 1-antitrypsin deficiency are described. In two of the cases the deficiency was genetically determined (cases 1 and 2), and each demonstrated unusual features of the disease. The liver in case 1 (homozygous) showed cholangiolar hyperplasia which has been recorded only once before. Case 2 (hetero...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.28.8.613
更新日期：1975-08-01 00:00:00
abstract：:A case of Cryptococcus neoformans meningitis is described in an HIV negative patient with undiagnosed systemic sarcoidosis. The patient presented with signs of meningitis together with generalised lymphadenopathy and hepatosplenomegaly. Cryptococcal meningitis was diagnosed on lumbar puncture. She was treated with int...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.12.928
更新日期：1999-12-01 00:00:00
abstract：:This best practice review examines four series of common primary care questions in laboratory medicine: (i) "minor" blood platelet count and haemoglobin abnormalities; (ii) diagnosis and monitoring of anaemia caused by iron deficiency; (iii) secondary hyperlipidaemia and hypertriglyceridaemia; and (iv) glycated haemog...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.200X.033944
更新日期：2006-08-01 00:00:00
abstract：AIMS:The aim of this study was to evaluate the mismatch repair gene expression and their correlation with NF-kB in patients with oral squamous cell carcinoma (SCC). METHODS:Total RNA was isolated from 28 biopsy samples. A control group was composed of 20 volunteers. Differential expression of hMSH2, hMSH6 and NF-kB ge...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2019-206161
更新日期：2020-05-01 00:00:00
abstract：:A case of B-CLL which was complicated by chronic renal failure due to leukaemic infiltration of the kidney is reported. Treatment with chlorambucil, prednisolone, and renal bed irradiation resulted in a substantial improvement in renal function which persisted until the the patient's death from marrow failure some eig...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.12.1131
更新日期：1993-12-01 00:00:00
abstract：:A large body of evidence supports the idea that certain adult stem cells, particularly those of bone marrow origin, can engraft at alternative locations, particularly when the recipient organ is damaged. Under strong and positive selection pressure these cells will clonally expand/differentiate, making an important co...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2003.010074
更新日期：2004-02-01 00:00:00
abstract：:This study has shown that different supplies/batches of orcein perform differently and may fail. The "natural" forms generally performed better although the most informative results were obtained with a "synthetic" product. Orcein dye solutions can be used soon after preparation and for up to 7 days without the need f...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.35.4.430
更新日期：1982-04-01 00:00:00
abstract：:Liver function was assessed in 38 Edinburgh haemophiliacs. Results before the introduction of NHS intermediate factor VIII concentrate from 1974 onwards were compared with values in 1979. Measurements of serum bile salts in 16 patients as well as conventional liver function tests gave useful evidence of deranged liver...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.34.1.17
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:Some uterine endometrioid adenocarcinomas exhibit a distinctive morphological phenotype characterised by the formation of microcystic, elongated and fragmented (MELF) glands. Immunohistochemical studies have suggested that MELF-type changes represent an epithelial-mesenchymal transition which has been associ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2009.069500
更新日期：2010-07-01 00:00:00
abstract：:Although the UK National Institute for Health and Care Excellence guidelines recommend that in patients with biopsy-proven invasive lobular carcinoma (ILC), preoperative MRI scan is considered, the accuracy of diagnosis of ILC in core biopsy of the breast has not been previously investigated. Eleven pathology laborato...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,多中心研究
doi：10.1136/jclinpath-2016-203886
更新日期：2016-12-01 00:00:00
abstract：:Mast cells were identified by electronmicroscopy in the alveolar wall of the lung in 20 subjects (10 normal, 10 abnormal). A quantitative and qualitative study was made of the mast cells. In the normal lung there was an average concentration of 350 mast cells/mm2 of alveolar wall and in the abnormal 523/mm2. Mast cell...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.34.12.1333
更新日期：1981-12-01 00:00:00
