Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.

abstract：:Mitochondrial membrane potentials and resistances have been determined under various metabolic conditions by the use of piezoelectric-driven microelectrodes. The mean state 3 potentials (about +19 mv) are higher than the potentials in state 4 (about +9 mv). DNP in uncoupling concentrations or KCN have no significant e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tupper JT,Tedeschi H

更新日期：1969-07-01 00:00:00

abstract：:Soil systems are being increasingly exposed to the interactive effects of biological invasions and climate change, with rising temperatures expected to benefit alien over indigenous species. We assessed this expectation for an important soil-dwelling group, the springtails, by determining whether alien species show br...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Janion-Scheepers C,Phillips L,Sgrò CM,Duffy GA,Hallas R,Chown SL

更新日期：2018-01-02 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:Heterotrimeric G proteins relay extracellular cues from heptahelical transmembrane receptors to downstream effector molecules. Composed of an alpha subunit with intrinsic GTPase activity and a betagamma heterodimer, the trimeric complex dissociates upon receptor-mediated nucleotide exchange on the alpha subunit, enabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slep KC,Kercher MA,Wieland T,Chen CK,Simon MI,Sigler PB

更新日期：2008-04-29 00:00:00

abstract：:Insights into the pathogenesis of migraine with aura may be gained from a study of human Ca(V)2.1 channels containing mutations linked to familial hemiplegic migraine (FHM). Here, we extend the previous single-channel analysis to human Ca(V)2.1 channels containing mutation V1457L. This mutation increased the channel o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tottene A,Fellin T,Pagnutti S,Luvisetto S,Striessnig J,Fletcher C,Pietrobon D

更新日期：2002-10-01 00:00:00

abstract：:Sympathetic ganglia and some peripheral tissues of adult guinea pig and cat were analyzed by the indirect immunofluorescence technique with antisera to catecholamine-synthesizing enzymes and some peptides. In the guinea pig, noradrenergic neurons could be subdivided into three populations containing respectively (i) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Hökfelt T,Anggård A,Terenius L,Elde R,Markey K,Goldstein M,Kimmel J

更新日期：1982-02-01 00:00:00

abstract：:Actin polymerization provides a powerful propulsion force for numerous types of cell motility. Although tremendous progress has been made in identifying the biochemical components necessary for actin-based motility, the precise biophysical mechanisms of force generation remain unclear. To probe the polymerization forc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Upadhyaya A,Chabot JR,Andreeva A,Samadani A,van Oudenaarden A

更新日期：2003-04-15 00:00:00

abstract：:d-amino acids are present in some peptides from amphibian skin. These residues are derived from the corresponding L-amino acids present in the respective precursors. From skin secretions of Bombinae, we have isolated an enzyme that catalyzes the isomerization of an L-Ile in position 2 of a model peptide to D-allo-Ile....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jilek A,Mollay C,Tippelt C,Grassi J,Mignogna G,Müllegger J,Sander V,Fehrer C,Barra D,Kreil G

更新日期：2005-03-22 00:00:00

abstract：:Cajal-Retzius (CR) cells are early-generated transient neurons and are important in the regulation of cortical neuronal migration and cortical laminar formation. Molecular entities characterizing the CR cell identity, however, remain largely elusive. We purified mouse cortical CR cells expressing GFP to homogeneity by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamazaki H,Sekiguchi M,Takamatsu M,Tanabe Y,Nakanishi S

更新日期：2004-10-05 00:00:00

abstract：:We introduced radioactive precursors directly into identified neurons of Aplysia californica. [(3)H]-Choline and L-[(3)H]tryptophan were injected with pressure into nerve cell bodies to study synthesis of acetylcholine and serotonin. We confirmed the cholinergic nature of R2, L10, and L11, identified neurons of the ab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenstadt M,Goldman JE,Kandel ER,Koike H,Koester J,Schwartz JH

更新日期：1973-12-01 00:00:00

abstract：:Thermus thermophilus possesses an aspartyl-tRNA synthetase (AspRS2) able to aspartylate efficiently tRNAAsp and tRNAAsn. Aspartate mischarged on tRNAAsn then is converted into asparagine by an omega amidase that differs structurally from all known asparagine synthetases. However, aspartate is not misincorporated into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker HD,Kern D

更新日期：1998-10-27 00:00:00

abstract：:Obesity is a major health problem worldwide, given its growing incidence and its association with a variety of comorbidities. Weight gain results from an increase in energy intake without a concomitant increase in energy expenditure. To combat the obesity epidemic, many studies have focused on the pathways underlying ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaspari RR,Reyna-Neyra A,Jung L,Torres-Manzo AP,Hirabara SM,Carrasco N

更新日期：2020-09-08 00:00:00

abstract：:The role of chemotaxis in the virulence of gastrointestinal pathogens is ill defined. Counterintuitively, nonchemotactic mutants of the polarly flagellated pathogen Vibrio cholerae greatly out-compete the wild-type strain during infection of the small intestine. We show that the out-competition phenotype is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler SM,Camilli A

更新日期：2004-04-06 00:00:00

abstract：:Glucagon-like peptide 1 (GLP-1) is a hormone derived from the preproglucagon molecule and is secreted by intestinal L cells. It is the most potent stimulator of glucose-induced insulin secretion and also suppresses in vivo acid secretion by gastric glands. A cDNA for the GLP-1 receptor was isolated by transient expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thorens B

更新日期：1992-09-15 00:00:00

abstract：:Uncouplers are molecules with protonophoric and ionophoric capabilities that mediate coupled cyclical transport of cations--a transport that takes precedence over all other coupled processes. Uncouplers form cation-containing complexes with electrogenic ionophores that potentiate cyclical transport of cations. The mol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kessler RJ,Vande Zande H,Tyson CA,Blondin GA,Fairfield J,Glasser P,Green DE

更新日期：1977-06-01 00:00:00

abstract：:A glycoprotein was isolated and purified to homogeneity from the serum of a patient with chronic non-A, non-B hepatitis. NaDodSO4/PAGE of the glycoprotein revealed a single major band at Mr approximately 77,000. Antibodies to this glycoprotein were shown to possess the following immunoreactivity: (i) they reacted by r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seto B,Gerety RJ

更新日期：1985-08-01 00:00:00

abstract：:The oligomerization of activated D- and L- and racemic guanosine-5'-phosphoro-2-methylimidazole on short templates containing D- and L-deoxycytidylate has been studied. Results obtained with D-oligo(dC)s as templates are similar to those previously reported for experiments with a poly(C) template. When one L-dC or two...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kozlov IA,Pitsch S,Orgel LE

更新日期：1998-11-10 00:00:00

abstract：:It was previously shown that coexpression of the lactose permease of Escherichia coli in two contiguous fragments leads to functional complementation. We demonstrate here that site-directed thiol crosslinking of coexpressed permease fragments can be used to determine helix proximity in situ without the necessity of pu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Kaback HR

更新日期：1996-12-10 00:00:00

abstract：:The cholesterol-dependent cytolysins (CDCs) constitute a large family of pore-forming toxins that function exclusively on cholesterol-containing membranes. A detailed analysis of the various stages in the cytolytic mechanism of three members of the CDC family revealed that significant depletion of cholesterol from the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giddings KS,Johnson AE,Tweten RK

更新日期：2003-09-30 00:00:00

abstract：:Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3'-end linked to a tyrosyl moiety and has been implicated in the repair of topoisomerase I (Top1)-DNA covalent complexes. TDP1 can also hydrolyze other 3'-end DNA alterations including 3'-phosphoglycolate and 3'-abasic sites, and ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das BB,Dexheimer TS,Maddali K,Pommier Y

更新日期：2010-11-16 00:00:00

abstract：:Activation of the mitogen-activated protein kinase (MAP kinase) isoforms ERK1 and ERK2 was investigated in rat adipocytes. Kinase activities were measured by using myelin basic protein as substrate after the isoforms were resolved by Mono Q chromatography or by immunoprecipitation with specific antibodies. Insulin inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sevetson BR,Kong X,Lawrence JC Jr

更新日期：1993-11-01 00:00:00

abstract：:Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dhonukshe P,Grigoriev I,Fischer R,Tominaga M,Robinson DG,Hasek J,Paciorek T,Petrásek J,Seifertová D,Tejos R,Meisel LA,Zazímalová E,Gadella TW Jr,Stierhof YD,Ueda T,Oiwa K,Akhmanova A,Brock R,Spang A,Friml J

更新日期：2008-03-18 00:00:00

abstract：:Plants produce a wide range of allelochemicals to defend against herbivore attack, and generalist herbivores have evolved mechanisms to avoid, sequester, or detoxify a broad spectrum of natural defense compounds. Successful arthropod pests have also developed resistance to diverse classes of pesticides and this adapta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dermauw W,Wybouw N,Rombauts S,Menten B,Vontas J,Grbic M,Clark RM,Feyereisen R,Van Leeuwen T

更新日期：2013-01-08 00:00:00

abstract：:In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciandra JJ,Subjeck JR,Hughes CS

更新日期：1984-08-01 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00

abstract：:We have cloned an additional member (GC-D) of the membrane receptor guanylyl cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] family that is specifically expressed in a subpopulation of olfactory sensory neurons. The extracellular, putative ligand-binding domain of the olfactory cyclase is similar in primary ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fülle HJ,Vassar R,Foster DC,Yang RB,Axel R,Garbers DL

更新日期：1995-04-11 00:00:00

abstract：:A power-law distribution of the length of perfectly conserved sequence from mouse/human whole-genome intersection and alignment is exhibited. Spatial correlations of these elements within the mouse genome are studied. It is argued that these power-law distributions and correlations are comprised in part by functional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salerno W,Havlak P,Miller J

更新日期：2006-08-29 00:00:00

abstract：:The c-ras family is a set of c-onc genes that are highly conserved in vertebrates. The genes in this family are homologous to the transforming genes of Harvey and Kirsten murine sarcoma viruses (v-Ha-ras and v-Ki-ras, respectively). Using an in situ molecular hybridization method, we detected three sites on the human ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jhanwar SC,Neel BG,Hayward WS,Chaganti RS

更新日期：1983-08-01 00:00:00

abstract：:We investigate energy transport through an alpha-aminoisobutyric acid-based 3(10)-helix dissolved in chloroform in a combined experimental-theoretical approach. Vibrational energy is locally deposited at the N terminus of the helix by ultrafast internal conversion of a covalently attached, electronically excited, azob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Botan V,Backus EH,Pfister R,Moretto A,Crisma M,Toniolo C,Nguyen PH,Stock G,Hamm P

更新日期：2007-07-31 00:00:00

abstract：:Patchy particles with shape complementarity can serve as building blocks for assembling colloidal superstructures. Alternatively, encoding information on patches using DNA can direct assembly into a variety of crystalline or other preprogrammed structures. Here, we present a tool where DNA is used both to engineer sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diaz A JA,Oh JS,Yi GR,Pine DJ

更新日期：2020-05-19 00:00:00