Fungal propagules in house dust. I. Comparison of analytic methods and their value as estimators of potential exposure.

Abstract:

:The presence of viable mold propagules in house dust was investigated by 10 different analytic methods, in order to determine to what extent different results are obtained when different analytic methods are used. Moreover, the value of this measurement as an estimator of the potential exposure to fungi in epidemiologic studies was assessed. Floor and mattress dust was sampled in 60 homes in The Netherlands during autumn 1990. For investigation of the variability in time, sampling was repeated in 20 homes after 6 weeks. Each analytic method is characterized by a unique combination of culture medium, suspension medium, and dilution step. The highest mean number of colony-forming units (CFU)/g dust was obtained by suspension of at least 100 mg dust in a peptone or sucrose solution in a ratio of 1:50 (w/w), followed by 10-fold dilution and plating on DG18 agar (geometric mean (GM) approximately 60,000 CFU/g dust). The lowest mean number of CFU/g dust was obtained by direct plating of 30 mg dust on V8 agar (GM approximately 5300 CFU/g dust). The mean coefficient of variation of duplicate analyses varied from 11%, for suspension in sucrose and plating on DG18 agar, to 27%, for suspension and dilution in sucrose in combination with V8 agar. The highest mean number of species isolated was obtained by direct plating of 30 mg dust on DG18 agar (mean number of species: 17). Suspension and dilution on DG18 or V8 agars yielded an average of approximately six species. In duplicate analyses, the mean percentage of agreement for the species isolated varied from approximately 35%, for suspension and dilution, to 60%, for direct plating.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Allergy

journal_title

Allergy

authors

Verhoeff AP,van Reenen-Hoekstra ES,Samson RA,Brunekreef B,van Wijnen JH

doi

10.1111/j.1398-9995.1994.tb01125.x

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

533-9

issue

7

eissn

0105-4538

issn

1398-9995

journal_volume

49

pub_type

杂志文章

相关文献

ALLERGY文献大全
  • Detection of specific IgE antibodies towards cat flea (Ctenocephalides felis felis) in patients with suspected cat allergy.

    abstract::Cat flea sensitivity is considered one of the most important skin diseases in cats and dogs. Cat fleas, however, are also a growing allergen problem for humans. Cat flea-specific IgE antibodies were studied in serum samples from 70 patients with suspected cat allergy, using RAST-based techniques and the nitrocellulose...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1987.tb02196.x

    authors: Rolfsen W,Schröder H,Tibell C,Tibell M

    更新日期:1987-04-01 00:00:00

  • Rhinitis symptoms and IgE sensitization as risk factors for development of later allergic rhinitis in adults.

    abstract:BACKGROUND:Rhinitis symptoms and IgE-sensitization often mismatch. Asymptomatic sensitization is an established risk factor for later rhinitis, whereas it is not clear whether rhinitis is a risk factor for later development of IgE-sensitization. OBJECTIVE:To investigate whether nonallergic rhinitis is a risk factor fo...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2006.01140.x

    authors: Bodtger U,Poulsen LK,Linneberg A

    更新日期:2006-06-01 00:00:00

  • Allergen-induced mediator release tests.

    abstract::The diagnosis of allergic reactions in clinical practice is based on both clinical history and the determination of specific immunoglobulin E (IgE), either in the serum or on skin mast cells. However, for various reasons, identification of the causative factors is not possible in all the cases. Moreover, not all aller...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1034/j.1398-9995.2003.00253.x

    authors: Demoly P,Lebel B,Arnoux B

    更新日期:2003-07-01 00:00:00

  • Effect of cetirizine on bronchial hyperresponsiveness in patients with seasonal allergic rhinitis and asthma.

    abstract::Although H1 antihistamine compounds (H1) are highly effective in the treatment of allergic rhinitis (AR), their role in the treatment of asthma is still controversial. Because a strong association between AR and bronchial hyperresponsiveness (BHR) has been reported, this study was designed to assess the effect of a ne...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1034/j.1398-9995.2001.00629.x

    authors: Aubier M,Neukirch C,Peiffer C,Melac M

    更新日期:2001-01-01 00:00:00

  • Crossed Immunoelectrophoresis analysis of bee venom. Enzymatic identification of antigens.

    abstract::Bee venom obtained by electrical stimulation has been analysed by crossed immunoelectrophoresis against high-titer rabbit antibodies. The antigenic analysis of bee venom revealed that the extract contained 17 antigens, which were detected in the crossed immunoelectrophoresis pattern after staining with Coomassie Brill...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1982.tb01910.x

    authors: Einarsson R,Annerhed A,Karlsson R,Olsson B,Renck B

    更新日期:1982-05-01 00:00:00

  • Gene expression analysis predicts insect venom anaphylaxis in indolent systemic mastocytosis.

    abstract:BACKGROUND:Anaphylaxis to insect venom (Hymenoptera) is most severe in patients with mastocytosis and may even lead to death. However, not all patients with mastocytosis suffer from anaphylaxis. The aim of the study was to analyze differences in gene expression between patients with indolent systemic mastocytosis (ISM)...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2010.02521.x

    authors: Niedoszytko M,Bruinenberg M,van Doormaal JJ,de Monchy JG,Nedoszytko B,Koppelman GH,Nawijn MC,Wijmenga C,Jassem E,Elberink JN

    更新日期:2011-05-01 00:00:00

  • Excess subcutaneous tissue may preclude intramuscular delivery when using adrenaline autoinjectors in patients with anaphylaxis.

    abstract::Intramuscular adrenaline is the gold standard treatment for anaphylaxis. Intramuscular injection provides more rapid and higher plasma concentrations than subcutaneous routes. Given the increasing epidemic of obesity patients are at increased risk of subcutaneous delivery, we therefore assessed the depth of subcutaneo...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12595

    authors: Johnstone J,Hobbins S,Parekh D,O'Hickey S

    更新日期:2015-06-01 00:00:00

  • Ebastine in allergic rhinitis and chronic idiopathic urticaria.

    abstract::Histamine is a key mediator in the development of allergy symptoms, and oral H(1)-antihistamines are among the most widely used treatments for symptomatic relief in conditions such as allergic rhinitis and chronic urticaria. Ebastine is a second-generation antihistamine which has been shown to be an effective treatmen...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.2008.01897.x

    authors: Sastre J

    更新日期:2008-12-01 00:00:00

  • Comparison of quantitative ELISA and semiquantitative Dustscreen for determination of Der p 1, Der f 1, and Fel d 1 in domestic dust samples.

    abstract:BACKGROUND:Quantitative ELISA kits used for the measurement of indoor allergens are time-consuming. Therefore, an immunodot assay (Dustscreen) has been introduced, so that results can be obtained within 4 h. In order to assess the potential applications of the new method, we compared quantitative ELISA methods and Dust...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2001.00117.x

    authors: Lau S,Schulz G,Sommerfeld C,Wahn U

    更新日期:2001-10-01 00:00:00

  • Experimental allergic conjunctivitis in inbred guinea pig strains with high respectively low bronchial allergic reactivity.

    abstract::Inbred guinea pigs selected for high (IMM/S) respectively low (IMM/R) responsiveness to ovalbumin (OA) as measured by induced respiratory anaphylaxis, were investigated for atopic immune responses of their conjunctival mucosa. IMM/S animals sensitized either by inhalation of OA, or by instillation of antigen into the ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1987.tb02208.x

    authors: Lundberg L,Bertelsen C,Zavaro A,Samra Z,Sompolinsky D

    更新日期:1987-05-01 00:00:00

  • IgE, IgG1, and IgG4 antibody responses to Blomia tropicalis in atopic patients.

    abstract:BACKGROUND:Allergens from house dust mites (HDMs), Dermatophagoides pteronyssinus and Blomia tropicalis are clinically relevant in atopic respiratory diseases in tropical countries. AIMS OF THE STUDY:To evaluate immunoglobulin (Ig)E, IgG1, and IgG4 antibody responses to B. tropicalis in Brazilian atopic patients. MET...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2005.00738.x

    authors: Pereira EA,Silva DA,Cunha-Júnior JP,Almeida KC,Alves R,Sung SJ,Taketomi EA

    更新日期:2005-03-01 00:00:00

  • Immunostimulatory DNA-based therapeutics for experimental and clinical allergy.

    abstract::Although allergen immunotherapy (IT) has been shown to modulate allergic hypersensitivities, its efficacy is limited. Recently, in various models of experimental allergy, a number of reagents which we have termed immunostimulatory DNA-based therapeutics have proven highly effective in both the prevention and reversal ...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1034/j.1398-9995.57.s72.12.x

    authors: Horner AA,Takabaysahi K,Zubeldia JM,Raz E

    更新日期:2002-01-01 00:00:00

  • Sleep disturbances in allergic diseases.

    abstract::Sleep is fundamental for physical and mental health as well as daytime performance and represents a crucial aspect in the personal representations of well-being. Sleep disturbances in patients with chronic disorders constitute a factor that contributes to exacerbate symptoms, complicate management, and affect mood and...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.2006.01221.x

    authors: Baiardini I,Braido F,Cauglia S,Canonica GW

    更新日期:2006-11-01 00:00:00

  • Enzyme-linked immunosorbent assay for detection of antibodies to the unmodified beta-lactam ring.

    abstract::Dose- and pH- dependent carbodiimide-mediated coupling of Penicillin-G to polystyrene microtiter-plates that leaves the beta-lactam ring unchanged is described. A new ELISA method was developed using Penicillin-G coated plates. The binding of 3 different monoclonal antibodies as well as human IgG antibodies of the IgG...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1993.tb00705.x

    authors: Klein GF,Stanzl U,Fritsch PO,Varga JM

    更新日期:1993-04-01 00:00:00

  • Single-dose inhaled budesonide in subjects with chronic asthma.

    abstract::In 30 stable asthmatics, a comparison was made between the changes in pulmonary function (FEV1, FVC, PEF, MEF75, MEF50 and MEF25) hourly for 9 h after a single dose of inhaled budesonide 1,600 micrograms, and placebo. All subjects used inhaled steroids daily; this medication was, however, withheld 8 days prior to the ...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1991.tb00619.x

    authors: Engel T,Dirksen A,Heinig JH,Nielsen NH,Weeke B,Johansson SA

    更新日期:1991-10-01 00:00:00

  • Volumetric aerobiological survey of conidial fungi in the North-East Netherlands. II. Comparison of aerobiological data and skin tests with mould extracts in an asthmatic population.

    abstract::A study was undertaken to see whether the principal airborne fungi in the North-East Netherlands were also found to be the most reactive in skin testing. Atmospheric samples were taken weekly with the Andersen sampler, from April 1981, up to and including, June 1983. At the same time skin tests of 833 patients referre...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1985.tb00214.x

    authors: Beaumont F,Kauffman HF,de Monchy JG,Sluiter HJ,de Vries K

    更新日期:1985-04-01 00:00:00

  • Ratio of myeloid and plasmacytoid dendritic cells and TH2 skew in CRS with nasal polyps.

    abstract:BACKGROUND:The role of myeloid and plasmacytoid dendritic cells and its consequences for the T(H)2 skew in chronic rhinosinusitis (CRS) with nasal polyps (CRSNP(+)) should be detailed. METHODS:In 18 CRS patients without nasal polyps (CRSNP(-)), 35 CRSNP(+) patients and 22 patients with nasal structural abnormalities w...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2009.02174.x

    authors: Kirsche H,Niederführ A,Deutschle T,Fuchs C,Riechelmann H

    更新日期:2010-01-01 00:00:00

  • Characterization of monocyte subtypes in the allergic form of atopic eczema/dermatitis syndrome.

    abstract:BACKGROUND:Monocyte (Mo) subsets exhibiting distinct phenotypic and functional properties identified in peripheral blood are assumed to be under the control of soluble factors from their surrounding micromilieu. Atopic eczema/dermatitis syndrome (AEDS) is accompanied by humoral and cellular alterations among which an i...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2002.23737.x

    authors: Novak N,Allam P,Geiger E,Bieber T

    更新日期:2002-10-01 00:00:00

  • Non-specific histamine release activity of busereline, LH-releasing hormone.

    abstract::Busereline acetate is an LH-RH agonist that was found to induce local reactions at the injection site or generalized minor skin reactions in some patients. Since these reactions may represent the first reaction of systemic anaphylaxis, we tested the non-specific histamine releasing activity of this drug by intradermal...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1988.tb01634.x

    authors: Bousquet J,Hédon B,Hejjaoui A,Chanal I,Michel FB

    更新日期:1988-10-01 00:00:00

  • Interpretation of subjective symptoms in double-blind placebo-controlled food challenges - interobserver reliability.

    abstract:BACKGROUND:Subjective symptoms after food challenges are difficult to interpret and no standard is available. We discuss a strategy for how to interpret a diary. Furthermore, the interobserver reliability is evaluated. METHODS:Diaries for 32 patients with subjective symptoms were used. The diaries were re-evaluated wi...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2003.00437.x

    authors: Gellerstedt M,Magnusson J,Gråjö U,Ahlstedt S,Bengtsson U

    更新日期:2004-03-01 00:00:00

  • Safety of rush insect venom immunotherapy. The results of a retrospective study in 178 patients.

    abstract:BACKGROUND:Venom immunotherapy (VIT) is regarded as an established and efficient treatment of Hymenoptera venom allergy. Different frequencies of side-effects have been reported in the literature, depending on the hyposensitization regimen (conventional outpatient programme, rush immunotherapy, ultra rush immunotherapy...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2003.00268.x

    authors: Wenzel J,Meissner-Kraemer M,Bauer R,Bieber T,Gerdsen R

    更新日期:2003-11-01 00:00:00

  • Nucleotide sequence of cDNA encoding the group II allergen of cocksfoot/orchard grass (Dactylis glomerata), Dac g II.

    abstract::Cocksfoot/orchard grass (Dactylis glomerata) anther cDNA clones encoding the group II allergen Dac g II were previously isolated on the basis of immunoreactivity of human, rabbit, and murine antibodies with a 24-kDa protein expressed as a fusion protein with beta-galactosidase. Nucleotide sequencing reveals an open re...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1993.tb00758.x

    authors: Roberts AM,Bevan LJ,Flora PS,Jepson I,Walker MR

    更新日期:1993-11-01 00:00:00

  • Immunological mechanisms of sublingual immunotherapy.

    abstract::Administration of allergen-specific immunotherapy by the oral route, sublingual immunotherapy (SLIT), has been shown to be effective, with an improved safety profile compared with subcutaneous administration. However, the precise mechanisms underlying the induction of immune tolerance by SLIT remain unclear. Contact o...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.2006.01159.x

    authors: Akdis CA,Barlan IB,Bahceciler N,Akdis M

    更新日期:2006-01-01 00:00:00

  • Mustard allergy confirmed by double-blind placebo-controlled food challenges: clinical features and cross-reactivity with mugwort pollen and plant-derived foods.

    abstract:BACKGROUND:Mustard IgE-mediated allergy is supposed to be a rare cause of food allergy, and its clinical features and cross-reactivities have not been fully elucidated. METHODS:A prospective study was carried out, recruiting mustard allergic patients, and paired control subjects. A clinical questionnaire was administe...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2005.00644.x

    authors: Figueroa J,Blanco C,Dumpiérrez AG,Almeida L,Ortega N,Castillo R,Navarro L,Pérez E,Gallego MD,Carrillo T

    更新日期:2005-01-01 00:00:00

  • Up-dosing with bilastine results in improved effectiveness in cold contact urticaria.

    abstract:BACKGROUND:Cold contact urticaria (CCU) is characterized by itchy wheal and flare responses due to the release of histamine and other pro-inflammatory mediators after exposure to cold. The treatment of choice is nonsedating antihistamines, dosages of which may be increased up to fourfold if standard doses are ineffecti...

    journal_title:Allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/all.12171

    authors: Krause K,Spohr A,Zuberbier T,Church MK,Maurer M

    更新日期:2013-07-01 00:00:00

  • Are overweight asthmatics more difficult to control?

    abstract::The relationship between asthma and obesity appears to be quite complex. The aim of this study was to assess the effect of excess weight on asthma control evolution in a cohort of asthmatics. A prospective database was set up, which enrolled adult asthmatics with persistent (mild, moderate or severe) asthma. The contr...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2005.00953.x

    authors: Saint-Pierre P,Bourdin A,Chanez P,Daures JP,Godard P

    更新日期:2006-01-01 00:00:00

  • Cytodestructive mechanisms provoked by food antigens. II. Antibody-dependent, allergic autocytotoxicity.

    abstract::Antibody-dependent, allergic autocytotoxicity (ACT) is an in vitro process through which WBC from patients and/or controls, passively sensitized with RAST positive serum, are damaged in the presence of corresponding food antigens. Studies among 13 patients, sensitive to cow milk, corn and wheat, and 13 asymptomatic co...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1985.tb00212.x

    authors: Podleski WK

    更新日期:1985-04-01 00:00:00

  • Quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the Basque region (Spain).

    abstract::We present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). Monoclonal antibody-based immunoassays were used to quantify Der p 1, Der f 1, Der 2, Lep d 1, and Fel d 1. T...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1995.tb01182.x

    authors: Echechipía S,Ventas P,Audícana M,Urrutia I,Gastaminza G,Polo F,Fernández de Corres L

    更新日期:1995-06-01 00:00:00

  • Efficacy of mequitazine in comparison with placebo assessed by ocular challenge with allergen in allergic conjunctivitis.

    abstract::Pharmacologic intervention in the management of allergic conjunctivitis was evaluated with different topical ocular agents in man. Their effect can be precisely assessed with the conjunctival provocation test (CPT). A potent specific H1-receptor antagonist, 0.05% mequitazine eye-drops, was tested in a double-blind ran...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1997.tb01028.x

    authors: Persi L,Dupin O,Arnaud B,Trinquand C,Michel FB,Bousquet J

    更新日期:1997-04-01 00:00:00

  • Circadian profiling reveals higher histamine plasma levels and lower diamine oxidase serum activities in 24% of patients with suspected histamine intolerance compared to food allergy and controls.

    abstract:BACKGROUND:Histamine intolerance is thought to trigger manifold clinical symptoms after ingesting histamine-rich food due to reduced activity of diamine oxidase (DAO). No study has hitherto systematically assessed daily fluctuations of histamine levels and DAO activities in symptomatic patients. The aim of the study wa...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.13361

    authors: Pinzer TC,Tietz E,Waldmann E,Schink M,Neurath MF,Zopf Y

    更新日期:2018-04-01 00:00:00