Abstract:
:Induction of heat shock/stress proteins is a key feature of a universal mechanism of cellular defence to injury known as the "stress response". The present study investigated whether heat shock protein expression correlates with the extent of neuronal injury inflicted by increasingly intense seizure activity. Limbic epilepsy was elicited by injecting intraperitoneally 8, 10 or 12 mg/kg kainic acid in adult Sprague-Dawley rats, resulting in graded degrees of seizure intensity and duration that closely correlated with the respective dose. Stress protein expression was investigated by immunocytochemistry and western blot analysis of microdissected brain areas with specific antibodies directed against representative members of three major classes of stress proteins, i.e. heat shock protein 72, heat shock protein 90 and heat shock protein 27, respectively. Heat shock protein 72 was absent in the brains of control animals, but markedly induced after limbic seizures in neurons of the limbic system, cortex, striatum and thalamus, with peak levels at 24 h. An increasing degree of seizure intensity caused a graded increase of heat shock protein 72 levels with a sequence reflecting the rank order of kainic acid susceptible hippocampal subpopulations. In contrast to heat shock protein 72, heat shock protein 90 was markedly expressed and equally abundant in all brain areas of untreated control animals and at any time point investigated following limbic seizures. Heat shock protein 27 was not detected in the brain of untreated animals nor following epilepsy. The present investigations demonstrate that the induction threshold of heat shock protein 72 in specific neuronal subpopulations clearly correlates with seizure intensity and duration. In addition, our experiments also define a narrow range of heat shock protein 72 expression with an upper limit beyond which heat shock protein 72 synthesis sharply declines. These findings reflect the risk of hippocampal neurons to undergo limbic seizure induced neuronal degeneration. It remains to be determined whether heat shock protein 72 expression is only a valuable marker for reversible neuronal injury or actually confers a neuroprotective effect.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Gass P,Prior P,Kiessling Mdoi
10.1016/0306-4522(95)92049-psubject
Has Abstractpub_date
1995-03-01 00:00:00pages
27-36issue
1eissn
0306-4522issn
1873-7544pii
0306-4522(95)92049-Pjournal_volume
65pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Electrophysiological records of unit activity were used to compare the effects of excitotoxic pallidal lesions and 6-hydroxydopamine-induced damage to the midbrain dopaminergic neurons on the discharge rates and patterns of the subthalamic neurons. Removal of the pallidal input induced a slight, but statistically sign...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00535-8
更新日期:1996-05-01 00:00:00
abstract::Peripheral and central diabetic neuropathies were studied in streptozotocin-diabetic rats, using behavioral, biochemical and electrophysiological techniques. Diabetic rats showed thermal hypoalgesia and decreasing motor nerve conduction velocity at 4 and 8 weeks of diabetes. In addition, amplitude of the evoked potent...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.02.047
更新日期:2009-05-05 00:00:00
abstract::A major input to the substantia nigra is from the 5-hydroxytryptamine-containing neurons in the dorsal raphe nucleus. In order to examine the morphology and distribution of this projection, rats were given injections of the anterograde tracers, Phaseolus vulgaris-leucoagglutinin or biocytin, in the dorsal raphe nucleu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90510-m
更新日期:1993-07-01 00:00:00
abstract::Spreading depolarizations are accompanied by transient changes in cerebral blood flow (CBF). In a post hoc analysis of previously studied control rats we analyzed CBF time courses after middle cerebral artery occlusion in the rat in order to test whether intra-ischemic flow, reperfusion, and different parameters of pe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.08.049
更新日期:2012-12-13 00:00:00
abstract::The cre/loxP system is used routinely to manipulate gene expression in the mouse nervous system. In order to delete genes specifically from the telencephalon, the Foxg1-cre line was created previously by replacing the intron-less Foxg1 coding region with cre, resulting in a Foxg1 heterozygous mouse. As the telencephal...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.06.012
更新日期:2007-08-24 00:00:00
abstract::Cognitive impairment is one of the common non-motor symptoms in Parkinson's disease (PD). The hippocampus is a critical structure for learning and memory processes. The abnormal synaptic plasticity in the hippocampus is suggested to be associated with cognitive dysfunction in PD. Voltage gated sodium channels (VGSCs) ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.08.024
更新日期:2019-10-15 00:00:00
abstract::In urethane-anesthetized rats with body temperature maintained at 39-40 degrees C, electrical stimulation of raphe magnus/pallidus/parapyramidal region within 0.5 mm of the ventral medullary surface reduced arterial blood flow to the tail cutaneous bed (measured with a chronically implanted Doppler ultrasonic flowmete...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00251-2
更新日期:2001-01-01 00:00:00
abstract::The electrical activity of locus coeruleus neurons was investigated during cortical spreading depression in urethane-anaesthetized rats. Cortical spreading depression was induced by a direct application of 1-3 M KCl solution to the surface of the cerebral cortex. The occurrence of cortical spreading depression was ass...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00679-3
更新日期:1997-06-01 00:00:00
abstract::We have recently identified a third subtype of glutamate vesicular transporter (VGLUT) named VGLUT3. In the present study, we provide a detailed account of the regional and cellular distributions of VGLUT3 in the rat brain, using specific nucleotide probes and antisera. The distribution of VGLUT3 protein was compared ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2003.10.039
更新日期:2004-01-01 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) produce widespread and complex effects on neocortex excitability. We studied how heteromeric nAChRs regulate inhibitory post-synaptic currents (IPSCs), in fast-spiking (FS) layer V neurons of the mouse frontal area 2 (Fr2). In the presence of blockers of ionotropic glutamate ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.10.045
更新日期:2017-01-06 00:00:00
abstract::Recent studies have demonstrated that total cellular levels of voltage-gated potassium channel subunits can change on a time scale of minutes in acute slices and cultured neurons, raising the possibility that rapid changes in the abundance of channel proteins contribute to experience-dependent plasticity in vivo. In o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.02.046
更新日期:2010-05-19 00:00:00
abstract::The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synapto...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.04.046
更新日期:2009-08-18 00:00:00
abstract::Electroconvulsive therapy has been commonly applied in the treatment of refractory depression, but its cognitive side effects are noticed and restrict its application. The molecular mechanisms underlying the side effects remain elusive, and there is no efficient prevention. By employing a recognized electroconvulsive ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.03.060
更新日期:2010-06-30 00:00:00
abstract::In vitro receptor autoradiography was used to localize, quantify and characterize [125I-Tyr8]bradykinin binding sites in all major spinal cord segments of normal rats and animals subjected to various chemical treatments and surgical lesions. [125I-Tyr8]bradykinin specific binding sites were predominantly located to su...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00161-b
更新日期:1995-10-01 00:00:00
abstract::The effects of systemically administered interleukin-1beta (1.0 microg), interleukin-6 (1.0 microg) and interleukin-2 (1.0 microg) on in vivo variations of monoamines were assessed in the nucleus accumbens. Administration of interleukin-1beta did not affect extracellular accumbal dopamine, provoked a modest rise of ho...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00271-1
更新日期:1999-01-01 00:00:00
abstract::3-Nitropropionic acid (3-NP), an irreversible inhibitor of succinate dehydrogenase, induced ATP depletion and both necrosis and apoptosis in human NT2-N neurons. Necrosis occurred predominantly within the first two days, and increased in a dose-dependent fashion with the concentration of 3-NP, whereas apoptosis was ob...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00177-x
更新日期:2002-01-01 00:00:00
abstract::Cannabinoid receptor type 1 (CB1)-dependent signaling in the brain is known to modulate food intake. Recent evidence has actually shown that CB1 can both inhibit and stimulate food intake in fasting/refeeding conditions, depending on the specific neuronal circuits involved. However, the exact brain sites where this bi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.01.005
更新日期:2014-03-28 00:00:00
abstract::A better understanding of BOLD responses stems from a better characterization of the brain's ability to metabolize glucose and oxygen. Non-invasive techniques such as functional magnetic resonance spectroscopy (fMRS) have thus been developed allowing for the reproducible assessment of metabolic changes during barrel c...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.01.034
更新日期:2017-03-27 00:00:00
abstract::Stimulation of the mu-opioid receptor (MOR) on nociceptors with fentanyl can produce hyperalgesia (opioid-induced hyperalgesia, OIH) and hyperalgesic priming, a model of transition to chronic pain. We investigated if local and systemic administration of biased MOR agonists (PZM21 and TRV130 [oliceridine]), which prefe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.10.015
更新日期:2018-12-01 00:00:00
abstract::Environmental enrichment of laboratory animals leads to multi-faceted changes to physiology, health and disease prognosis. An important and under-appreciated factor in enhancing cognition through environmental manipulation may be improved basic sensory function. Previous studies have highlighted changes in cortical se...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.07.029
更新日期:2010-10-13 00:00:00
abstract::The auditory system continuously monitors the environment for irregularities in an automatic, preattentive fashion. This is presumably accomplished by two mechanisms: a sensory mechanism detects a deviant sound on the basis of differential refractoriness of neural populations sensitive to the standard and deviant soun...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.039
更新日期:2013-12-03 00:00:00
abstract::Brain structural connectivity is known to be altered in cases of intrauterine growth restriction and premature birth, although the specific effect of maternal nutritional restriction, a common burden in human populations, has not been assessed yet. Here we analyze the effects of maternal undernutrition during pregnanc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.03.049
更新日期:2018-06-01 00:00:00
abstract::Ghrelin induces orexigenic behavior by activation of growth hormone secretagogue 1 receptors (GHSRs) in the ventral tegmental area (VTA) as well as hypothalamus, suggesting the involvement of mesolimbic dopamine system in the action of ghrelin. The present study aimed to identify neuronal mechanisms by which periphera...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.03.086
更新日期:2009-07-07 00:00:00
abstract::Cholesterol is an essential substance for maintaining normal structure and function of the brain. But unfortunately, a long-term high-cholesterol diet can lead to a variety of pathological changes of the brain such as β-amyloid (Aβ) accumulation, Tau hyperphosphorylation, reactive gliosis, neuroinflammation, neuronal ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.12.039
更新日期:2016-03-01 00:00:00
abstract::Secretion of neurotrophins is critical for the delivery of neurotrophic support. Brain-derived neurotrophic factor is targeted to a regulated secretory pathway in neurons as well as the neurosecretory AtT-20 cells. Here, we show that pertussis toxin, which inactivates Gi and Go G proteins, inhibits up to 50% of the re...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00309-2
更新日期:2000-01-01 00:00:00
abstract::The visual word form area (VWFA) has been consistently identified as a crucial structure in visual word processing. Nevertheless, it is controversial whether the VWFA represents external visual information (e.g., case information) of visual words. To address that question, we functionally localized VWFA at the group l...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.10.040
更新日期:2021-01-01 00:00:00
abstract::Estrogens influence neuronal activity and are important for normal brain functions. Effects of estrogens on seizures are contradictory. It is commonly accepted that estrogens may increase neuronal excitability and thus mediate proconvulsant effects. However, clinical and animal data show that estrogen may also have no...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2005.07.005
更新日期:2006-01-01 00:00:00
abstract::The heme oxygenase (HO) enzyme system has been shown to participate in nociceptive signaling in a number of different models of pain. In these experiments we investigated the role of the HO type 2 (HO-2) isozyme in tolerance to the analgesic effects of morphine, and the hyperalgesia and allodynia which are measurable ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00483-4
更新日期:2003-01-01 00:00:00
abstract::The C57BL/Wld s mouse is a mutant strain of mouse that shows greatly slowed Wallerian degeneration both in the central and peripheral nervous system. Using immunohistochemistry, immunofluorescence and Western blotting, we have investigated the distribution of the chimeric Wld s protein and its different components in ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.06.078
更新日期:2005-01-01 00:00:00
abstract::The purpose of the present study was to investigate, by use of in vivo microdialysis technique, the regulatory role of galanin on acetylcholine (ACh) release in the CA1, CA3, and dentate gyrus (DG) subregions of rat dorsal and ventral hippocampus. In the ventral hippocampus, local infusions of galanin (1.5 nmol) into ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.09.035
更新日期:2011-12-01 00:00:00