Emergence of a synaptic neuronal network within primary striatal cultures seeded in serum-free medium.

Abstract:

:In order to investigate the basic cellular mechanisms involved in neuronal interactions within the striatum, we prepared a primary striatal cell culture from rat fetal brain in chemically defined medium. Using morphological and whole-cell recording methods, we observed that an intensive neuritic elongation with a progressive build up of a sodium-dependent electrogenesis occurred during the first week of culture. Morphologically mature synapses began to develop after 10 days in vitro. By this time, most of the neurons (82 +/- 9%) received spontaneously synaptic potentials, which led them to fire (71 +/- 11%). The spontaneous firing was prevented by cadmium (200 microM) and tetrodotoxin (5 microM), which suggested that a Ca(2+)-dependent release of neurotransmitters was involved in the synaptic activation. We further obtained evidence that GABA, and to a lesser extent acetylcholine, contributed to these spontaneous synaptic potentials. At 15 days in vitro, it was possible to observe up to four synaptic contacts on a given dendrite. By this time, whole-cell recordings performed on pairs of neurons showed that the mature neurons were interconnected by excitatory synapses. As the number of synapses increased, the striatal neurons gradually formed a large network in which spontaneous activity developed, which tended to be organized into synchronized bursting patterns.

journal_name

Neuroscience

journal_title

Neuroscience

authors

Kowalski C,Crest M,Vuillet J,Pin T,Gola M,Nieoullon A

doi

10.1016/0306-4522(94)00453-c

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

979-93

issue

4

eissn

0306-4522

issn

1873-7544

pii

030645229400453C

journal_volume

64

pub_type

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