Abstract:
:This study examines the ultrastructural features of diffuse intimal thickenings of human coronary arteries and correlates structural heterogeneity along the radial axis with the distribution and known actions of transforming growth factor beta 1 (TGF-beta 1). Morphometric and immunohistochemical data were collected from thickenings of varying widths, sampled at autopsy from 19 persons ranging in age from 3 months to 81 years. Thickenings were characterised by an inner proteoglycan layer (PGL), up to approximately 70 mm wide, and an underlying variable-width musculofibrous layer (MFL). The PGL was characterised by low volume fractions (v/fs) for collagen, elastin, basement membranes and cells and a high v/f for matrix space; v/fs for the MFL components were more evenly distributed. Proteoglycans were visualised by ruthenium red staining, quantified and sized. Densities of large (versican; > 20 nm) and small (< 20 nm) granules changed little across intimal thickenings. Mean diameters of matrix space granules increased with increasing intimal thickness and notably were significantly (p < 0.001) larger in the PGL than the MFL. In contrast, diameters of collagen-associated small granules (decorin) did not differ between the PGL and MFL. TGF-beta 1 staining was detected in 70% of vessels examined and occurred almost exclusively in the PGL, although showed a patchy distribution. Both the distribution of TGF-beta 1 and its known differential effects on versican and decorin synthesis suggest that it may play a significant role in the formation and maintenance of the PGL.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Merrilees MJ,Beaumont Bdoi
10.1159/000159008subject
Has Abstractpub_date
1993-09-01 00:00:00pages
293-302issue
5eissn
1018-1172issn
1423-0135journal_volume
30pub_type
杂志文章abstract::This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159097
更新日期:1995-07-01 00:00:00
abstract::Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081804
更新日期:2004-11-01 00:00:00
abstract::Protein localization in endothelial cells is tightly regulated to create distinct signaling domains within their tight spatial restrictions including luminal membranes, abluminal membranes, and interendothelial junctions, as well as caveolae and calcium signaling domains. Protein localization in endothelial cells is a...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000512618
更新日期:2021-01-27 00:00:00
abstract::Inflammation is a highly regulated process with common but also specific characteristics in each tissue affected. Recruitment of leukocytes from the blood to the injured tissue is an important early step in the inflammatory cascade. This review highlights the role of connexins (Cxs) in the regulation of both acute and...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000316942
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00
abstract::The effect of shear stress at the endothelium in the attenuation of the noradrenaline-induced constriction of the femoral vascular bed perfused at a constant blood flow was investigated in 16 anesthetized cats. It is known that the adrenergic vasoconstriction of the femoral vascular bed is considerably greater at a co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097750
更新日期:2007-01-01 00:00:00
abstract::Actions of the novel Ca2+ antagonist, Ro 40-5967, which displays unusual efficacy against endothelin (ET)-induced contractions, were studied in isolated vascular muscle cells (VMCs) using the fluorescent protein kinase C (PKC) indicator, BODIPY 12 alpha-phorbol ester 13 beta-acetate (PBA-BODIPY). High-sensitivity (pho...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159134
更新日期:1996-01-01 00:00:00
abstract::Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are cytoplasmic adaptor proteins of the TNF/interleukin (IL)-1/Toll-like receptor superfamily. Ligands of this family such as TNFα, CD40L, and IL-1β promote chronic inflammatory processes such as atherosclerosis and restenosis, the latter being a common a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000501615
更新日期:2019-01-01 00:00:00
abstract::The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025655
更新日期:1999-07-01 00:00:00
abstract::Although most physiologically important processes are regulated through negative feedback loops and numerous factors regulating endothelial apoptosis are identified, little is known about negative feedback mechanisms for endothelial apoptosis. Here we describe the release of soluble anti-apoptotic activity by endothel...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000111072
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND/AIMS:Dysfunction of endothelial progenitor cell (EPCs) contributes to diabetic vascular disease. We reported that downregulated miR-126 in diabetic patients causes EPC dysfunction. The study was designed to investigate how high glucose (HG) and advanced glycation end products (AGEs) regulate miR-126 expressi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000448713
更新日期:2016-01-01 00:00:00
abstract::Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000353287
更新日期:2013-01-01 00:00:00
abstract:AIM:Vascular remodeling following injury substantially accounts for restenosis and adverse clinical outcomes. In this study, we investigated the role of the giant scaffold protein Ahnak1 in vascular healing after endothelial denudation of the murine femoral artery. METHODS:The spatiotemporal expression pattern of Ahna...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000464287
更新日期:2017-01-01 00:00:00
abstract::During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000070711
更新日期:2003-03-01 00:00:00
abstract::In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000317399
更新日期:2011-01-01 00:00:00
abstract::The aim of this study was to verify that subcutaneous lymph node transplantation inducing lymphatic regeneration is possible in healthy adult rats, in analogy to results obtained in other species. This rat model was used to determine the effects of lymph node fragmentation as well as sheep erythrocytes and platelet-ri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000194269
更新日期:2009-01-01 00:00:00
abstract::Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081247
更新日期:2004-09-01 00:00:00
abstract::The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159237
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000258905
更新日期:2010-01-01 00:00:00
abstract::The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. Heparin has been widely reported to inhibit the growth of VSMC in culture and intimal VSMC in some animal models of vascular hyperplasia. Clinical trials with heparin, howe...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025616
更新日期:1998-11-01 00:00:00
abstract::Conduit arteries of hypertensive rats are thicker and stiffer than those of normotensive controls. The possible role played by collagen type I and II subtypes in the mechanism of arterial stiffness remains unknown. The carotid and aortic arterial wall of rats of Japanese (Wistar-Kyoto and spontaneously hypertensive ra...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025637
更新日期:1999-03-01 00:00:00
abstract::Neointima formation participates in the pathophysiology of atherosclerosis and restenosis. Proliferation and migration of vascular smooth muscle cells (VSMC) are initial responses to vascular injury. The aim of the present study was to assess the effect of gliotoxin, an inhibitor of nuclear factor (NF)-kappaB, on migr...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000176043
更新日期:2009-01-01 00:00:00
abstract::Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159106
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND/AIMS:Laser Doppler flowmetry (LDF) was used to determine the influence of acute myocardial infarction (AMI) and of successful reperfusion treatment on basal skin blood flow and its oscillatory components. METHODS:Skin LDF was performed on all extremities in 58 patients 4-9 days after AMI (Killip class I), a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000110208
更新日期:2008-01-01 00:00:00
abstract::Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000481781
更新日期:2017-01-01 00:00:00
abstract::Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097977
更新日期:2007-01-01 00:00:00
abstract::Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass g...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000376548
更新日期:2015-01-01 00:00:00
abstract::We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051081
更新日期:2001-09-01 00:00:00
abstract:UNLABELLED:Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000381097
更新日期:2015-01-01 00:00:00