Abstract:
:We have examined the effects of low pH and the selectivity of the capsaicin antagonist capsazepine on single sensory fibres innervating the guinea-pig trachea in vitro, and on the whole isolated vagus nerve. Application of a pH 5 solution for 1 min to the exposed receptive fields of single fibres caused excitation of all C-fibres tested but had no effect on A delta-fibres. Capsazepine (1 microM) perfused onto the receptive field for 5 min produced a reversible inhibition of both low pH- and capsaicin (60 nM)-evoked firing of C-fibres. In contrast, capsazepine had no effect on responses of C-fibres to bradykinin (0.1 microM) or of A delta-fibres to hypertonic saline. Perfusion of tissues with zero-calcium Krebs' solution containing trypsin produced denudation of the epithelium. In these tissues responses to low pH and capsaicin were unchanged and, moreover, the inhibitory effect of capsazepine against low pH and capsaicin was maintained. C- and A delta-fibre responses to bradykinin and hypertonic saline were similarly unaffected by epithelium removal. Perfusion of the whole guinea-pig vagus nerve with capsaicin (0.3 microM) or pH 5 buffer caused depolarization. However, in this preparation prior perfusion with capsazepine (1 microM) abolished responses to capsaicin whilst low pH-evoked depolarization was unchanged. These data show that capsazepine is a specific antagonist of proton- and capsaicin-evoked activation of the peripheral endings of sensory nerves in the guinea-pig airways, and suggest the release by protons of an endogenous ligand for the capsaicin receptor that does not originate from the epithelium.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Fox AJ,Urban L,Barnes PJ,Dray Adoi
10.1016/0306-4522(95)00115-ysubject
Has Abstractpub_date
1995-08-01 00:00:00pages
741-52issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(95)00115-Yjournal_volume
67pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Delta opioid receptor (DOR) is essential for neuronal survival against hypoxic/ischemic damages. However, current understanding on how DOR activation affects astrocytic functions under ischemia remains incomplete. The present study investigated the astroglial responses to [d-Ala2, d-Leu5] enkephalin (DADLE) (a selecti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.067
更新日期:2011-09-29 00:00:00
abstract::The mechanism of neuropeptide secretion induced by bradykinin from capsaicin-sensitive afferents was studied in guinea-pig atria. Both the inotropic response induced by bradykinin (0.1 microM) in the electrically driven isolated guinea-pig left atria and the bradykinin (10 microM)-induced release of calcitonin gene-re...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90062-9
更新日期:1990-01-01 00:00:00
abstract::Neuronal gap junctions in the brain, providing intercellular electrotonic signal transfer, have been implicated in physiological and behavioral correlates of learning and memory. In connexin31.1 (Cx31.1) knockout (KO) mice the coding region of the Cx31.1 gene was replaced by a LacZ reporter gene. We investigated the i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.01.077
更新日期:2008-05-02 00:00:00
abstract::Estrogens influence neuronal activity and are important for normal brain functions. Effects of estrogens on seizures are contradictory. It is commonly accepted that estrogens may increase neuronal excitability and thus mediate proconvulsant effects. However, clinical and animal data show that estrogen may also have no...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2005.07.005
更新日期:2006-01-01 00:00:00
abstract::The pedunculopontine nucleus (PPN) shows altered electrophysiological and anatomic characteristics in Parkinson's disease (PD), but little is known about the effect of 6-hydroxydopamine (6-OHDA) lesion and levodopa (L-DOPA) therapy on the relationship between spike and local field potential (LFP) activities in the PPN...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.01.044
更新日期:2019-04-15 00:00:00
abstract::While systemic capsaicin in adult rats is known to reduce substance P and somatostatin in primary sensory nerves, it is still unknown if it also affects the production of these peptides at the genetic level. Therefore, we examined the effects of systemically administered capsaicin on the expression of the beta-preprot...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00334-x
更新日期:1997-01-01 00:00:00
abstract::The sec6/8 (exocyst) complex is implicated in targeting of vesicles for regulated exocytosis in various cell types and is believed to play a role in synaptogenesis and brain development. We show that the subunits sec6 and sec8 are present at significant levels in neurons of adult rat brain, and that immunoreactivity f...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00065-4
更新日期:2003-01-01 00:00:00
abstract::Synaptic plasticity is considered a physiological substrate for learning and memory [Lynch MA (2004) Long-term potentiation and memory. Physiol Rev 84:87-136] that contributes to maladaptive learning in drug addiction [Schoenbaum G, Roesch MR, Stalnaker TA (2006) Orbitofrontal cortex, decision-making and drug addictio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.03.061
更新日期:2009-06-30 00:00:00
abstract::The function of N-methyl-d-aspartate (NMDA) receptors in primary afferents remains controversial, in particular regarding their ability to evoke substance P release in the spinal cord. The objective of this study was, first, to confirm that substance P release evoked by NMDA is mediated by NMDA receptors in primary af...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.01.009
更新日期:2010-03-31 00:00:00
abstract::A new mammalian neuromuscular preparation is introduced for physiology and microscopy of all sorts: the intrinsic muscle of the mouse ear. The great utility of this preparation is demonstrated by illustrating how it has permitted us to develop a wholly new technique for staining muscle T-tubules, the critical conducti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.07.028
更新日期:2020-07-15 00:00:00
abstract::Experiment 1 investigated the effects of catecholaminergic deafferentation or cell body lesions of the amygdala on fear conditioning to explicit and contextual cues. Bilateral infusions of quinolinic acid mainly damaged neurons within the basolateral region of the amygdala. 6-Hydroxydopamine infusions at the same coor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90379-3
更新日期:1991-01-01 00:00:00
abstract::In the present study, we investigated the possible participation of the endocannabinoid system in the basolateral amygdala and N-methyl-d-aspartate (NMDA) or GABA-A receptor neurotransmission in the ventral tegmental area in the memory consolidation impairment induced by morphine administration. To measure memory form...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.03.036
更新日期:2017-06-14 00:00:00
abstract::Previous studies have shown that amphetamine (AMPH) markedly activates dopaminergic projection areas, together with some important limbic nuclei. However, a global picture of the brain areas activated is lacking and the contribution of the dose of the drug and individual differences to this global brain activation is ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.04.020
更新日期:2010-07-14 00:00:00
abstract::Seizures produced by pilocarpine given i.p. to rats provide an animal model for studying the initiation, spread and generalisation of convulsive activity within the forebrain. Pilocarpine, 380 mg/kg, produces a sequence of behavioural and electroencephalographic alterations indicative of motor limbic seizures and stat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90179-x
更新日期:1986-05-01 00:00:00
abstract::The assessment of binge ethanol-induced neuronal activation, using c-Fos immunoreactivity (IR) as a marker of neuronal activity, is typically accomplished via forced ethanol exposure, such as intraperitoneal injection or gavage. Neuronal activity using a voluntary binge-like drinking model, such as "drinking-in-the-da...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.10.027
更新日期:2017-12-26 00:00:00
abstract::The distribution of gene expression domains during development constitutes a novel tool for the identification of distinct brain regions. This is particularly useful in the brain of amphibians where cell migration is very limited and most neurons organize in a periventricular layer. Here we report the expression patte...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00326-3
更新日期:2002-01-01 00:00:00
abstract::A few hours after peripheral axons of cutaneous afferent neurons have been transected, some of their novel endings become excitable by physical or chemical stimuli. It has been assumed that these axon endings preferentially respond to those stimuli which have excited their previous receptive endings. We studied the pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00325-5
更新日期:1999-01-01 00:00:00
abstract::Previous studies have clearly demonstrated that the immediate-early gene, c-fos can regulate, through its protein product Fos, the expression of the pro-opiomelanocortin gene. In the present study, immunohistochemistry for Fos and beta-endorphin was used to assess the basal activity of hypothalamic pro-opiomelanocorti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00208-0
更新日期:1999-01-01 00:00:00
abstract::The analgesic effect of alpha-2 adrenergic receptor (α2AR) agonists, which relieve chronic neuropathic pain, is highly variable among individuals. Here, we used a mouse model of spared nerve injury (SNI) to show that treatment time after the establishment of neuropathic pain was important for the variability in the an...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.12.021
更新日期:2020-12-25 00:00:00
abstract::Neuronal differentiation is a critical developmental process that determines accurate synaptic connection and circuit wiring. A wide variety of naturally occurring compounds have been shown as promising drug leads for the generation and differentiation of neurons. Here we report that a diarylheptanoid from the plant A...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.07.019
更新日期:2015-09-10 00:00:00
abstract::The ontogeny of the calcitonin gene-related peptide in the neuron system of the rat brain stem was investigated by means of the indirect immunofluorescence technique. Calcitonin gene-related peptide-like immunoreactivity was first detected in the fibers of the nucleus of spinal tract trigeminal nerve on gestational da...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90108-x
更新日期:1988-09-01 00:00:00
abstract::Estrogen exerts many of its receptor-mediated neuroprotective functions through the activation of various intracellular signal transduction pathways including the mitogen activating protein kinase (MAPK), phospho inositol-3 kinase and protein kinase C pathways. Here we have used a hippocampal slice culture model of ka...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.12.029
更新日期:2005-01-01 00:00:00
abstract::Cellular distribution of creatine kinase BB-isoenzyme and tubulin was investigated immunohistochemically with tissue sections from various areas of the gerbil brain. Both the reaction for creatine kinase BB-isoenzyme and tubulin visualized nerve cell bodies, dendrites and axons. While the reaction for tubulin was simi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90183-0
更新日期:1984-07-01 00:00:00
abstract::The majority of work examining the nucleus accumbens core (NAc) has focused on functions pertaining to behaviors guided by appetitive outcomes. These studies have pointed to the NAc as being critical for motivating behavior toward desirable outcomes. For example, we have recently shown that lesions of the NAc impaired...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.11.028
更新日期:2014-01-31 00:00:00
abstract::Ciliary neurotrophic factor is known to exert both survival and differentiative actions on a number of neuronal populations of the peripheral and central nervous systems. In this study we have compared the trophic effects of ciliary neurotrophic factor and nerve growth factor on developing septal neurons of the rat in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00590-2
更新日期:1995-06-01 00:00:00
abstract::The present study was undertaken to quantify selected neuropeptides (thyrotropin releasing hormone, substance P, methionine and leucine enkephalin) in the cervical spinal cord and other regions of the central nervous system of Wobbler mice by radioimmunoassays during several stages of the motoneuron disease compared w...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90393-g
更新日期:1992-09-01 00:00:00
abstract::We investigated functional organization of the vagus nerve (N. X)- and glossopharyngeal nerve (N. IX)-related nuclei in the embryonic rat brainstem and compared their development and spatial distribution patterns, using multiple-site optical recording with a fast voltage-sensitive dye, NK2761. Intact brainstem prepara...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.019
更新日期:2011-09-29 00:00:00
abstract::Despite a long history of interrogation, the functional organization of motor cortex remains obscure. A major barrier has been the inability to measure and perturb activity with sufficient resolution to reveal clear functional elements within motor cortex and its associated circuits. Increasingly, the mouse has been e...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2020.07.054
更新日期:2020-12-01 00:00:00
abstract::Neurons in the region of dorsomedial hypothalamus are involved in the organization of the physiological responses to emotional stress. We have recently shown that the cardiovascular response evoked by activation of dorsomedial hypothalamus neurons is largely dependent on a synaptic relay with the lateral/dorsolateral ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.12.041
更新日期:2006-01-01 00:00:00
abstract::Rats were given bilateral lesions of the motor cortex on the tenth day of life, and then received a daily subcutaneously injection of either basic fibroblast growth factor (FGF-2) or vehicle for 7 consecutive days. In adulthood, they were trained and assessed on a skilled forelimb reaching task. Although all lesion gr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.04.006
更新日期:2005-01-01 00:00:00