Abstract：
:The rod and cone transducins are specific G proteins originally thought to be present only in photoreceptor cells of the vertebrate retina. Transducins convert light stimulation of photoreceptor opsins into activation of cyclic GMP phosphodiesterase (reviewed in refs. 5-7). A transducin-like G protein, gustducin, has been identified and cloned from rat taste cells. We report here that rod transducin is also present in vertebrate taste cells, where it specifically activates a phosphodiesterase isolated from taste tissue. Furthermore, the bitter compound denatonium in the presence of taste-cell membranes activates transducin but not Gi. A peptide that competitively inhibits rhodopsin activation of transducin also blocks taste-cell membrane activation of transducin, arguing for the involvement of a seven-transmembrane-helix G-protein-coupled receptor. These results suggest that rod transducin transduces bitter taste by coupling taste receptor(s) to taste-cell phosphodiesterase. Phosphodieterase-mediated degradation of cyclic nucleotides may lead to taste-cell depolarization through the recently identified cyclic-nucleotide-suppressible conductance.
journal_name
Naturejournal_title
Natureauthors
Ruiz-Avila L,McLaughlin SK,Wildman D,McKinnon PJ,Robichon A,Spickofsky N,Margolskee RFdoi
10.1038/376080a0subject
Has Abstractpub_date
1995-07-06 00:00:00pages
80-5issue
6535eissn
0028-0836issn
1476-4687journal_volume
376pub_type
杂志文章相关文献
NATURE文献大全abstract：:Anti-inflammatory glucocorticoids inhibit prostaglandin (PG) biosynthesis by preventing arachidonic acid release from phospholipids rather than inhibiting the cyclooxygenase. As in other cells, this steroid action depends on receptor occupation and de novo protein/RNA biosynthesis. We have previously shown in guinea p...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/287147a0
更新日期：1980-09-11 00:00:00
abstract：:Over the past decade, ice loss from the Greenland Ice Sheet increased as a result of both increased surface melting and ice discharge to the ocean. The latter is controlled by the acceleration of ice flow and subsequent thinning of fast-flowing marine-terminating outlet glaciers. Quantifying the future dynamic contrib...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12068
更新日期：2013-05-09 00:00:00
abstract：:Proteomics, the large-scale analysis of proteins, will contribute greatly to our understanding of gene function in the post-genomic era. Proteomics can be divided into three main areas: (1) protein micro-characterization for large-scale identification of proteins and their post-translational modifications; (2) 'differ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/35015709
更新日期：2000-06-15 00:00:00
abstract：:While progress in fusion research continues with magnetic and inertial confinement, alternative approaches--such as Coulomb explosions of deuterium clusters and ultrafast laser-plasma interactions--also provide insight into basic processes and technological applications. However, attempts to produce fusion in a room t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03575
更新日期：2005-04-28 00:00:00
abstract：:Modern chitons (Mollusca: Polyplacophora) possess a highly conserved skeleton of eight shell plates (valves) surrounded by spicules or scales, and fossil evidence suggests that the chiton skeleton has changed little since the first appearance of the class in the Late Cambrian period (about 500 million years before pre...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02548
更新日期：2004-05-20 00:00:00
abstract：:For unknown reasons, autoimmune diseases such as type 1 diabetes develop after prolonged periods of inflammation of mononuclear cells in target tissues. Here we show that progression of pancreatic islet inflammation to overt diabetes in nonobese diabetic (NOD) mice is driven by the 'avidity maturation' of a prevailing...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35021081
更新日期：2000-08-17 00:00:00
abstract：:Methane is an important greenhouse gas that is emitted from multiple natural and anthropogenic sources. Atmospheric methane concentrations have varied on a number of timescales in the past, but what has caused these variations is not always well understood. The different sources and sinks of methane have specific isot...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/nature11461
更新日期：2012-10-04 00:00:00
abstract：:Rous sarcoma virus (RSV) is an acutely oncogenic avian retrovirus which induces sarcomas in animals and transforms fibroblasts in cell culture. Genetic analysis indicates that the viral src gene (v-src) mediates neoplastic transformation. The product of v-src is a 60,000 molecular weight (MW) phosphoprotein (pp60v-src...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/302837a0
更新日期：1983-04-28 00:00:00
abstract：:Activity-dependent change in the efficacy of transmission is a basic feature of many excitatory synapses in the central nervous system. The best understood postsynaptic modification involves a change in responsiveness of AMPAR (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor)-mediated currents follo...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35013064
更新日期：2000-05-25 00:00:00
abstract：:In response to environmental cues that promote IP3 (inositol 1,4,5-trisphosphate) generation, IP3 receptors (IP3Rs) located on the endoplasmic reticulum allow the 'quasisynaptical' feeding of calcium to the mitochondria to promote oxidative phosphorylation. However, persistent Ca2+ release results in mitochondrial Ca2...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature22965
更新日期：2017-06-22 00:00:00
abstract：:Ever since Stephen Paget's 1889 hypothesis, metastatic organotropism has remained one of cancer's greatest mysteries. Here we demonstrate that exosomes from mouse and human lung-, liver- and brain-tropic tumour cells fuse preferentially with resident cells at their predicted destination, namely lung fibroblasts and ep...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature15756
更新日期：2015-11-19 00:00:00
abstract：:The biological importance of DNA methylation for gene expression in eukaryotes is becoming increasingly evident, and a direct role of methylation in gene expression has been suggested by an analysis of the infectivity of integrated retroviral genomes in a transfection assay. These studies, however, did not address whe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/304275a0
更新日期：1983-07-21 00:00:00
abstract：:Cosmological models in which dark matter consists of cold elementary particles predict that the dark halo population should extend to masses many orders of magnitude below those at which galaxies can form1-3. Here we report a cosmological simulation of the formation of present-day haloes over the full range of observe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2642-9
更新日期：2020-09-01 00:00:00
abstract：:The Kaposi's sarcoma-associated herpesvirus (KSHV/HHV8) is a gamma-2 herpesvirus that is implicated in the pathogenesis of Kaposi's sarcoma and of primary effusion B-cell lymphomas (PELs). KSHV infects malignant and progenitor cells of Kaposi's sarcoma and PEL, it encodes putative oncogenes and genes that may cause Ka...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/34193
更新日期：1998-01-01 00:00:00
abstract：:Monoclonal antibodies can now be genetically engineered and endowed with new properties. In the future, gene technology could enable antigen-binding fragments to be made by exploiting repertoires of variable domain genes derived from immunized animals and expressed in bacteria. How readily can this approach be extende...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/349293a0
更新日期：1991-01-24 00:00:00
abstract：:An experiment by the Gates Foundation is food for thought for other research agencies. ...
journal_title：Nature
pub_type： 社论
doi：10.1038/4551150a
更新日期：2008-10-30 00:00:00
abstract：:In budding yeast many genes are expressed under cell-cycle control in late G1. These include a large group of DNA synthesis genes, the HO gene involved in mating-type switching, CTS1 (chitinase) and also CLN1 and CLN2 (ref. 4) encoding G1 cyclins. Two factors, encoded by the SWI4 and SWI6 genes, are required for HO (r...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/357505a0
更新日期：1992-06-11 00:00:00
abstract：:Stable maintenance of gene regulatory programs is essential for normal function in multicellular organisms. Epigenetic mechanisms, and DNA methylation in particular, are hypothesized to facilitate such maintenance by creating cellular memory that can be written during embryonic development and then guide cell-type-spe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13458
更新日期：2014-09-04 00:00:00
abstract：:Fas is a type-I membrane protein that transduces an apoptotic signal. Binding of Fas ligand or agonistic anti-Fas antibody to Fas kills the cells by apoptosis. Studies in the nematode Caenorhabditis elegans have suggested that proteases such as interleukin-1 beta-converting enzyme (ICE) or the product of the C. elegan...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/375078a0
更新日期：1995-05-04 00:00:00
abstract：:Transmembrane sodium-ion gradients provide energy that can be harnessed by 'secondary transporters' to drive the translocation of solute molecules into a cell. Decades of study have shown that such sodium-coupled transporters are involved in many physiological processes, making them targets for the treatment of numero...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature08143
更新日期：2009-05-21 00:00:00
abstract：:Magma intrusions and eruptions commonly produce abrupt changes in seismicity far from magma conduits that cannot be associated with the diffusion of pore fluids or heat. Such 'swarm' seismicity also migrates with time, and often exhibits a 'dog-bone'-shaped distribution. The largest earthquakes in swarms produce after...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature00997
更新日期：2002-09-05 00:00:00
abstract：:With the discovery1 of superconductivity at 203 kelvin in H3S, attention returned to conventional superconductors with properties that can be described by the Bardeen-Cooper-Schrieffer and the Migdal-Eliashberg theories. Although these theories predict the possibility of room-temperature superconductivity in metals th...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1201-8
更新日期：2019-05-01 00:00:00
abstract：:The TGF-beta/activin/BMP cytokine family signals through serine/threonine kinase receptors, but how the receptors transduce the signal is unknown. The Mad (Mothers against decapentaplegic) gene from Drosophila and the related Sma genes from Caenorhabditis elegans have been genetically implicated in signalling by membe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/381620a0
更新日期：1996-06-13 00:00:00
abstract：:The crystal structure of a solid controls the interactions between the electronically active units and thus its electronic properties. In the high-temperature superconducting copper oxides, only one spatial arrangement of the electronically active Cu(2+) units-a two-dimensional square lattice-is available to study the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09120
更新日期：2010-07-08 00:00:00
abstract：:Inhibitory neurotransmission mediated by GABA(A) receptors can be modulated by the endogenous neurosteroids, allopregnanolone and tetrahydro-deoxycorticosterone. Neurosteroids are synthesized de novo in the brain during stress, pregnancyand after ethanol consumption, and disrupted steroid regulation of GABAergic trans...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05324
更新日期：2006-11-23 00:00:00
abstract：:The angle between the spin of a star and the orbital planes of its planets traces the history of the planetary system. Exoplanets orbiting close to cool stars are expected to be on circular, aligned orbits because of strong tidal interactions with the stellar convective envelope. Spin-orbit alignment can be measured w...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature24677
更新日期：2018-01-25 00:00:00
abstract：:Comparative mapping, which compares the location of homologous genes in different species, is a powerful tool for studying genome evolution. Comparative maps suggest that rates of chromosomal change in mammals can vary from one to ten rearrangements per million years. On the basis of these rates we would expect 84 to ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/46555
更新日期：1999-11-25 00:00:00
abstract：:Overexploitation of renewable resources today has a high cost on the welfare of future generations. Unlike in other public goods games, however, future generations cannot reciprocate actions made today. What mechanisms can maintain cooperation with the future? To answer this question, we devise a new experimental para...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13530
更新日期：2014-07-10 00:00:00
abstract：:Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome. How the intasome interfaces with chromosomal DNA, which exists in the form of nucleosomal arrays, is currently unknown. Here we show that the prototype foamy virus (PFV) intasome ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature14495
更新日期：2015-07-16 00:00:00
abstract：:Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanen...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10163
更新日期：2011-06-15 00:00:00
