Adeno-associated virus 2-mediated gene transfer and functional expression of the human granulocyte-macrophage colony-stimulating factor.

Abstract:

:It is becoming increasingly clear that the adeno-associated virus 2 (AAV)-based vector system may prove to be useful for high-efficiency gene transfer in human cells as well as for potential gene therapy in humans. A recombinant AAV vector containing the gene for a human hematopoietic growth factor, granulocyte-macrophage colony-stimulating factor (GM-CSF), was constructed and used to infect COS-1 cells, a monkey kidney cell line. COS-1 cells infected with the recombinant virus, but not mock-infected cells, expressed high levels of the human GM-CSF gene transcripts. Furthermore, in co-cultivation experiments with the recombinant virus-infected cells, but not in those with mock-infected cells, active proliferation of a GM-CSF-dependent human megakaryocytic leukemia cell line, M07e, could be obtained in the absence of exogenously added GM-CSF. The recombinant GM-CSF protein released into the supernatant was biologically active in progenitor cell assays carried out with primary human hematopoietic cells, and this activity was specifically abrogated by treatment of the supernatant with anti-GM-CSF antibodies. This recombinant virus may be potentially useful in the management and gene therapy of a variety of malignant disorders in the human hematopoietic system.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Luo F,Zhou SZ,Cooper S,Munshi NC,Boswell HS,Broxmeyer HE,Srivastava A

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

1261-7

issue

12

eissn

0301-472X

issn

1873-2399

journal_volume

23

pub_type

杂志文章
  • Antibodies to the beta 1-integrin chain, CD44, or ICAM-3 stimulate adhesion of blast colony-forming cells and may inhibit their growth.

    abstract::Early hematopoietic progenitor cells adhere to bone marrow stromal cells (BMSCs) mainly through VLA-4/VCAM-1 interactions. However, many adhesion molecules are expressed by these cell types. Cell adhesion molecules not only mediate adhesion; some are also capable of triggering cellular signaling events. These signals ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Oostendorp RA,Spitzer E,Reisbach G,Dörmer P

    更新日期:1997-04-01 00:00:00

  • Importance of marrow dose on posttransplant outcome in acute leukemia: models derived from patients autografted with mafosfamide-purged marrow at a single institution.

    abstract::Several prospective randomized trials in acute myelocytic leukemia (AML) documented a lower relapse rate with autologous bone marrow transplantation (ABMT) than with conventional chemotherapy. However, they also identified some transplant difficulties, such as failure to collect sufficient numbers of stem cells, slow ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(99)00121-6

    authors: Gorin NC,Labopin M,Laporte JP,Douay L,Lopez M,Lesage S,Fouillard L,Isnard F,Jouet JP,Bellal N,Perot C,Van Den Akker J,Bauters F,Najman A

    更新日期:1999-12-01 00:00:00

  • The relationships between Epstein-Barr virus latent membrane protein 1 and regulatory T cells in Hodgkin's lymphoma.

    abstract:OBJECTIVE:Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is expressed by the malignant cells of about 30% of cases of Hodgkin's lymphoma (HL) and is therefore a potential target for immune attack. Given the predominantly immunosuppressive nature of HL infiltrating lymphocytes (HLILs) and the ability ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2007.01.030

    authors: Marshall NA,Culligan DJ,Tighe J,Johnston PW,Barker RN,Vickers MA

    更新日期:2007-04-01 00:00:00

  • Down-regulation of retroviral transgene expression during differentiation of progenitor-derived dendritic cells.

    abstract:OBJECTIVE:Hematopoietic progenitor cells are a promising source for generation of genetically modified dendritic cells. A prerequisite for using these cells in therapeutic approaches is stable vector-mediated transgene expression during and after cell maturation. We investigated the expression of enhanced green fluores...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(01)00778-0

    authors: Lindemann C,Schilz AJ,Emons B,Baum C,Löw R,Fauser AA,Kuehlcke K,Eckert HG

    更新日期:2002-02-01 00:00:00

  • Kinase signaling and targeted therapy for primary myelofibrosis.

    abstract::The myeloproliferative neoplasms (MPNs) are somatic mutation-driven hematologic malignancies characterized by bone marrow fibrosis and the accumulation of atypical megakaryocytes with reduced polyploidization in the primary myelofibrosis subtype of the MPNs. Increasing evidence points to a dominant role of abnormal me...

    journal_title:Experimental hematology

    pub_type: 杂志文章,评审

    doi:10.1016/j.exphem.2016.12.007

    authors: Yang Q,Crispino JD,Wen QJ

    更新日期:2017-04-01 00:00:00

  • Selective proliferation of lymphoid cells from lineage-c-Kit+ Sca-1+ cells by a clonal bone marrow stromal cell line.

    abstract::To understand the regulatory mechanisms involved in the development of hematopoietic stem cells, we cultured lineage-negative, c-Kit+ Sca-1+ stem cells sorted from bone marrow cells by a fluorescence-activated cell sorter (FACS) on layers of bone marrow stromal cell lines established from SV40 T-antigen gene transgeni...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Koguma M,Matsuda K,Okuyama R,Yanai N,Obinata M

    更新日期:1998-04-01 00:00:00

  • HSP27 protects AML cells against VP-16-induced apoptosis through modulation of p38 and c-Jun.

    abstract:OBJECTIVE:To investigate 1) the signal transduction pathways affected by heat shock protein 27 (HSP27) expression; and 2) the expression and regulation of HSP27 in acute myeloid leukemia (AML). MATERIALS AND METHODS:RNA interference studies for HSP27 in leukemic TF-1 cells were used to investigate the effects on downs...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2005.03.009

    authors: Schepers H,Geugien M,van der Toorn M,Bryantsev AL,Kampinga HH,Eggen BJ,Vellenga E

    更新日期:2005-06-01 00:00:00

  • GPIIb (CD41) integrin is expressed on mast cells and influences their adhesion properties.

    abstract:OBJECTIVES:GPIIb integrin expression has been found on platelets and megakaryocytes, and more recently on immature hematopoietic progenitors. We set out to investigate expression of GPIIb in other hematopoietic cell lineages and, having detected it on mast cells, aimed to determine what possible role it might perform. ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2005.01.011

    authors: Berlanga O,Emambokus N,Frampton J

    更新日期:2005-04-01 00:00:00

  • Hemangioblastic characteristics of fetal bone marrow-derived Flk1(+)CD31(-)CD34(-) cells.

    abstract:OBJECTIVE:To investigate whether Flk1(+)CD31(-)CD34(-) cells isolated from fetal bone marrow (BM) have characteristics of hemangioblasts, i.e., progenitors of endothelial and hematopoietic cells. MATERIALS AND METHODS:Mononuclear cells from fetal BM were negatively sorted by CD45, GlyA, and CD34 micromagnetic beads, t...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(03)00087-0

    authors: Guo H,Fang B,Liao L,Zhao Z,Liu J,Chen H,Hsu SH,Cui Q,Zhao RC

    更新日期:2003-07-01 00:00:00

  • Contribution of STAT3 to the activation of survivin by GM-CSF in CD34+ cell lines.

    abstract:OBJECTIVE:Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to specifically stimulate proliferation of CD34(+) hematopoietic progenitor cells. Although signal transducers and activators of transcription 3 (STAT3) is believed essential for transduction of GM-CSF-induced cell proliferation, the sig...

    journal_title:Experimental hematology

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.exphem.2007.03.007

    authors: Gu L,Chiang KY,Zhu N,Findley HW,Zhou M

    更新日期:2007-06-01 00:00:00

  • Distinct roles of IL-7 and stem cell factor in the OP9-DL1 T-cell differentiation culture system.

    abstract:OBJECTIVE:The OP9-DL1 culture system is an in vitro model for T-cell development in which activation of the Notch pathway by Delta-like 1 promotes differentiation of mature T cells from progenitors. The roles of specific cytokines in this culture system have not been well defined, and controversy regarding the role of ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2006.08.001

    authors: Wang H,Pierce LJ,Spangrude GJ

    更新日期:2006-12-01 00:00:00

  • T-cell apoptosis induced by granulocyte colony-stimulating factor is associated with retinoblastoma protein phosphorylation and reduced expression of cyclin-dependent kinase inhibitors.

    abstract::Peripheral blood progenitor cells (PBPC) mobilized by granulocyte colony-stimulating factor (G-CSF) promptly engraft allogeneic recipients after myeloablative chemotherapy for hematologic malignancies. Surprisingly, no exacerbation of acute graft-vs-host disease has been observed despite a 10-fold higher T-cell conten...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(01)00617-8

    authors: Rutella S,Pierelli L,Rumi C,Bonanno G,Marone M,Sica S,Capoluongo E,Ameglio F,Scambia G,Leone G

    更新日期:2001-04-01 00:00:00

  • Cell-cycle kinetics and VSV-G pseudotyped retrovirus-mediated gene transfer in blood-derived CD34+ cells.

    abstract::As hematopoietic stem and progenitor cells have a low mitotic index, we have quantitated the impact of cytokine combinations on cell cycling of CD34+ cells and, using VSV-G pseudotyped retroviral vectors, correlated our findings with ex vivo gene transfer. We tested nine different combinations of cytokines for inducti...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Agrawal YP,Agrawal RS,Sinclair AM,Young D,Maruyama M,Levine F,Ho AD

    更新日期:1996-05-01 00:00:00

  • Effects of rhG-CSF (filgrastim) on the recovery of hematopoiesis after high-dose chemotherapy and autologous peripheral blood stem cell transplantation in children: a report from the Children's Cancer and Leukemia Study Group of Japan.

    abstract::In a nonrandomized study, hematopoietic recovery kinetics were evaluated in 98 consecutive patients who underwent high-dose chemotherapy without total body irradiation (TBI) and autologous peripheral blood stem cell transplantation (PBSCT). Fifty-three patients received recombinant human granulocyte colony-stimulating...

    journal_title:Experimental hematology

    pub_type: 临床试验,杂志文章

    doi:

    authors: Suzue T,Takaue Y,Watanabe A,Kawano Y,Watanabe T,Abe T,Kuroda Y,Matsushita T,Kikuta A,Iwai A

    更新日期:1994-11-01 00:00:00

  • Induction of differentiation and enhancement of vincristine sensitivity of human erythroleukemia HEL cells by vesnarinone, a positive inotropic agent.

    abstract::We examined the effect of vesnarinone, an oral cardiotonic, on the growth and differentiation of human myeloid leukemia cells. Vesnarinone alone markedly induced erythroid differentiation of HEL cells. All-trans-retinoic acid also induced erythroid differentiation of the cells, and the differentiation was greatly enha...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Sato S,Tomoyasu S,Okabe-Kado J,Hozumi M,Tsuruoka N,Nakai S,Adachi M,Honma Y

    更新日期:1996-01-01 00:00:00

  • Thyroid hormones enhance hypoxia-induced erythropoietin production in vitro.

    abstract::Effects of thyroid hormones on the production of erythropoietin (Epo) were investigated in isolated perfused rat kidneys and in the human hepatoma cell line, HepG2. Epo protein was measured by radioimmunoassay. L-triiodothyronine and L-thyroxine stimulated hypoxia-induced Epo formation both in the kidney and in HepG2 ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Fandrey J,Pagel H,Frede S,Wolff M,Jelkmann W

    更新日期:1994-03-01 00:00:00

  • Pluripotent stem cells with normal or reduced self renewal survive lethal irradiation.

    abstract::Transfusion with 10,000 or 20,000 marrow cells resulted in 30+ days survival of 15%-50% of mice exposed to an Ld90 or LD100 or radiation. The use of congenic mice with alloenzyme markers permitted the identification of host and donor cells in the peripheral blood of transfused animals. Donor cells were present initial...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Brecher G,Neben S,Yee M,Bullis J,Cronkite EP

    更新日期:1988-08-01 00:00:00

  • Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytes.

    abstract:OBJECTIVE:We have recently reported that 20% O2 significantly enhances total megakaryocyte (Mk) number, polyploidy, and proplatelet formation compared to 5% O2 in culture. In order to further elucidate the regulatory role of pO2 on megakaryocytopoiesis, we conducted a kinetic study of the expression of surface markers ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(01)00658-0

    authors: Mostafa SS,Papoutsakis ET,Miller WM

    更新日期:2001-07-01 00:00:00

  • Effects of recombinant granulocyte colony-stimulating factor (rG-CSF) and recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) on acute radiation hematopoietic injury in mice.

    abstract::We have attempted to evaluate in vivo effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on acute radiation hematopoietic injury in mice. BDF1 mice, irradiated with 7.5-Gy x-rays, were injected i.p. twice daily for 10 days with 10(5) U recombinant hum...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Tanikawa S,Nakao I,Tsuneoka K,Nara N

    更新日期:1989-09-01 00:00:00

  • A 17-kd polypeptide, sensitive to bacterial collagenase, is synthesized by bone marrow macrophages.

    abstract::A novel polypeptide with a molecular weight of 17 kd (17k protein) was identified in bone marrow cell cultures. The synthesis of 17k protein is elevated in cell cultures maintained under Dexter conditions, which support myelopoiesis. The predominance of macrophages in the stromal layer of these cultures and the observ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Ruland LJ 3rd,Quesenberry PJ,Balian G

    更新日期:1990-10-01 00:00:00

  • Removal of hematopoietic cells and macrophages from mouse bone marrow cultures: isolation of fibroblastlike stromal cells.

    abstract::A method is described that permits the removal of hematopoietic cells and macrophages from mouse bone marrow cultures. The method is based on the difference in effect of extracellular ATP4- ions (ATP in the absence of divalent, complexing cations) on cells of hematopoietic origin, including macrophages, and of nonhema...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Modderman WE,Vrijheid-Lammers T,Löwik CW,Nijweide PJ

    更新日期:1994-02-01 00:00:00

  • Cyclooxygenase-2 is essential for normal recovery from 5-fluorouracil-induced myelotoxicity in mice.

    abstract::Cyclooxygenase (COX) plays a key regulatory role in prostaglandin synthesis. COX-2 is inducible and is the major isoform of inflammatory cells. COX-2-deficient mice were shown to have normal basal hematopoiesis and hematology. We hypothesized that COX-2 induction plays a role in the recovery phase of 5-fluorouracil (5...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(99)00087-9

    authors: Lorenz M,Slaughter HS,Wescott DM,Carter SI,Schnyder B,Dinchuk JE,Car BD

    更新日期:1999-10-01 00:00:00

  • Suppression of apoptosis and granulocyte colony-stimulating factor-induced differentiation by an oncogenic form of Cbl.

    abstract:OBJECTIVE:The retroviral oncogene v-Cbl causes pre-B cell lymphomas and myeloid leukemias in mice, and its Drosophila homologue is oncogenic, causing enhanced receptor tyrosine kinase signaling. The human Cbl gene resides at 11q23. The aim of this study is to determine the effect of oncogenic Cbl on growth-regulating r...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(01)00647-6

    authors: Sinha S,Jancarik J,Roginskaya V,Rothermund K,Boxer LM,Corey SJ

    更新日期:2001-06-01 00:00:00

  • Progenipoietin-1: a multifunctional agonist of the granulocyte colony-stimulating factor receptor and fetal liver tyrosine kinase-3 is a potent mobilizer of hematopoietic stem cells.

    abstract:OBJECTIVE:Progenipoietin-1 is an agonist of both the granulocyte colony-stimulating factor and fetal liver tyrosine kinase-3 receptors capable of inducing the proliferation of multiple hematopoietic cell lineages. The potential of progenipoietin-1 to mobilize transplantable hematopoietic stem cells into the peripheral ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(01)00675-0

    authors: Fleming WH,Mulcahy JM,McKearn JP,Streeter PR

    更新日期:2001-08-01 00:00:00

  • Gene expression profile of primary human CD34+CD38lo cells differentiating along the megakaryocyte lineage.

    abstract:OBJECTIVE:To identify genes involved in megakaryopoiesis, high-density oligonucleotide microarrays were used to compare transcript profiles from undifferentiated CD34+CD38lo cells and culture-derived megakaryocytes (MKs). MATERIALS AND METHODS:Megakaryocyte differentiation was achieved in vitro by inducing primary hum...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2004.04.002

    authors: Shim MH,Hoover A,Blake N,Drachman JG,Reems JA

    更新日期:2004-07-01 00:00:00

  • The BMP pathway: A unique tool to decode the origin and progression of leukemia.

    abstract::The microenvironment (niche) governs the fate of stem cells (SCs) by balancing self-renewal and differentiation. Increasing evidence indicates that the tumor niche plays an active role in cancer, but its important properties for tumor initiation progression and resistance remain to be identified. Clinical data show th...

    journal_title:Experimental hematology

    pub_type: 杂志文章,评审

    doi:10.1016/j.exphem.2018.02.005

    authors: Zylbersztejn F,Flores-Violante M,Voeltzel T,Nicolini FE,Lefort S,Maguer-Satta V

    更新日期:2018-05-01 00:00:00

  • Kynurenine, by activating aryl hydrocarbon receptor, decreases erythropoietin and increases hepcidin production in HepG2 cells: A new mechanism for anemia of inflammation.

    abstract::It is known that inadequate erythropoietin (EPO) production contributes to the pathogenesis of anemia of inflammation, although the exact molecular mechanism is unknown. Aryl hydrocarbon receptor (AhR) may compete with hypoxia-inducible factor 2α (HIF-2α), the master regulator of EPO production, for binding with HIF-1...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2015.08.010

    authors: Eleftheriadis T,Pissas G,Antoniadi G,Liakopoulos V,Stefanidis I

    更新日期:2016-01-01 00:00:00

  • A randomized multicenter comparison of CD34(+)-selected progenitor cells from blood vs from bone marrow in recipients of HLA-identical allogeneic transplants for hematological malignancies.

    abstract:OBJECTIVE:Peripheral blood progenitor cells (PBPC) have been established as an alternative source of hematopoietic stem cells for allogeneic transplantation, but an increased incidence of both acute and chronic graft-vs-host disease (GVHD) has become apparent. We performed a prospective randomized trial comparing bone ...

    journal_title:Experimental hematology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1016/s0301-472x(03)00195-4

    authors: Cornelissen JJ,van der Holt B,Petersen EJ,Vindelov L,Russel CA,Höglund M,Maertens J,Schouten HC,Braakman E,Steijaert MM,Zijlmans MJ,Slaper-Cortenbach I,Boogaerts MA,Löwenberg B,Verdonck LF

    更新日期:2003-10-01 00:00:00

  • Inhibition of human bone marrow-derived stem cell colony formation (CFU-E, BFU-E, and CFU-GM) following in vitro exposure to organophosphates.

    abstract::Cholinergic toxicity of organophosphate insecticides is regarded as the principal health hazard associated with both human and animal exposures. Recent studies indicate that these pesticides may have important effects on both the immune and hematopoietic systems. In the present study, human bone marrow cells were expo...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Gallicchio VS,Casale GP,Watts T

    更新日期:1987-12-01 00:00:00

  • A multifactorial analysis of umbilical cord blood, adult bone marrow and mobilized peripheral blood progenitors using the improved ML-IC assay.

    abstract:OBJECTIVE:Assays that can evaluate the potential of individual human hematopoietic stem cells (HSC) are still lacking. We previously developed the myeloid-lymphoid initiating cell (ML-IC) assay that enumerates single CD34(+) cells that generate long-term culture-initiating (LTC-IC) and NK-initiating (NK-IC) daughter ce...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2004.10.016

    authors: Theunissen K,Verfaillie CM

    更新日期:2005-02-01 00:00:00