Abstract：
:This study was undertaken in order to establish the presence of pluripotential neuroblasts in the developing chick CNS. This has been suggested by our previous observations that expression of emerging neuronal phenotypes in the chick embryo CNS is affected by exposure to neurotrophic substances (i.e., GHRH, SRIF, NGF, EGF, muscle-derived factors) or neurotoxins such as ethanol. We have proposed that one mechanism whereby these substances elicit their effects is by shifting phenotypic expression in populations of pluripotential neuroblasts. In order to establish the presence of significant populations of pluripotential neuroblasts, cultures obtained from 3-day-old whole chick embryos (E3WE) were double-stained with antibodies to markers specific for four neuronal phenotypes in various permutations. Cultures at 6 DIV were tested for the presence of tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), gamma-aminobutyric acid (GABA), and somatostatin (SRIF) alone, and in various combinations. We observed a colocalization of all phenotypic markers within neuronal perikarya and processes in more than fifty percent of neuronal cells in these cultures. These data suggest that developing neuroblasts at this stage of embryogenesis possess the machinery necessary to adopt multiple neuronal phenotypes. The colocalization of neurotransmitter proteins in early neuroblasts (60 hr of embryogenesis) supports the recent concept that these substances themselves may influence phenotypic expression and also supports our idea that microenvironmental factors (i.e., ethanol, growth factors) provide signals which affect emerging phenotypes.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Kentroti S,Vernadakis Adoi
10.1002/jnr.490410516subject
Has Abstractpub_date
1995-08-01 00:00:00pages
696-707issue
5eissn
0360-4012issn
1097-4547journal_volume
41pub_type
杂志文章abstract：:Cathepsin D-like activity in homogenates of five brain areas of 3-month-old and 24-month-old Fischer 344 rats was measured. With hemoglobin as substrate at pH 3.2, more than 90% of the activity was inhibited by pepstatin. In each area studied, activity was more than twice as high in the old rat brain: 140-160% higher ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490230412
更新日期：1989-08-01 00:00:00
abstract：:Cultured Schwann cells treated with heregulin growth factor require costimulation with a cyclic adenosine monophosphate-elevating agent to produce maximal cell proliferation. Gene chip expression analysis was used to identify genes that are induced or repressed when Schwann cells are treated with heregulin and/or fors...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10679
更新日期：2003-08-15 00:00:00
abstract：:GM1 ganglioside has been shown to stimulate recovery of the damaged dopamine system under a number of different circumstances. In addition to rescue of damaged dopamine neurons, the present study assessed the ability of GM1 to enhance the synthesis of dopamine in remaining nigrostriatal neurons following 1-methyl-4-ph...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490420113
更新日期：1995-09-01 00:00:00
abstract：:The striatum plays an important role in learning, selecting, and executing actions. As a major input hub of the basal ganglia, it receives and processes a diverse array of signals related to sensory, motor, and cognitive information. Aberrant neural activity in this area is implicated in a wide variety of neurological...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24519
更新日期：2019-12-01 00:00:00
abstract：:Multiple sclerosis (MS) is an immune-mediated demyelinating disorder of unknown etiology. Both genetic-susceptibility and environment exposures, including vitamin D deficiency, Epstein-Barr viral and Herpesvirus (HHV-6) infections are strongly implicated in the activation of T cells and MS-pathogenesis. Despite precis...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24322
更新日期：2018-12-01 00:00:00
abstract：:Dissociated embryonic rat ventral mesencephalon containing the developing A8-A10 dopamine (DA) neurons was cultured alone or in the presence of a 10 mm segment of adult rat sciatic nerve that had been explanted and maintained in separate culture for 72 hours prior to introduction to mesencephalic cultures. Nerve segme...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270318
更新日期：1990-11-01 00:00:00
abstract：:Toll-like receptors (TLRs) play an essential role in initiating intracellular type I interferon (IFN)-mediated innate immunity against viral infections. We examined whether human neuronal cells (primary human neurons, NT2-N and CHP-212 cells) express TLRs and mount type I IFN-mediated innate immunity against herpes si...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22110
更新日期：2009-10-01 00:00:00
abstract：:Mutations in the beta-amyloid precursor protein (APP) gene cause familial Alzheimer's disease (AD). Although amyloid beta peptide (Abeta) is the principal constituent of senile plaques in AD, other cleavage products of APP are also implicated in playing a role in the pathogenesis of AD. C-terminal fragments of APP (AP...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000515)60:4<565::AID-JNR
更新日期：2000-05-15 00:00:00
abstract：:Cultures of rat C6 rat glioma cells exhibit a diminished response to isoproterenol and forskolin after being treated with phorbol 12,13-dibutyrate (PDbU). An IC50 for PDbU of 38 +/- 5 nM and 62 +/- 8 nM was observed in the isoproterenol and forskolin response, respectively. Similarly, C6 cultures exhibited a diminishe...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250110
更新日期：1990-01-01 00:00:00
abstract：:There are two types of calcium-activated neutral protease (CANP), m-CANP and mu-CANP, following the nomenclature of Suzuki et al to show that each requires mM and microM Ca2+, respectively, for its activation. We found mu-CANP activity in a crude CANP fraction extracted from the peripheral nerve, which degraded the ne...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130306
更新日期：1985-01-01 00:00:00
abstract：:The use of trimethadione (TMO) as a protector in hyperbaric oxygen toxicity in the conscious rat has been examined in detail. The oxidation-reduction state of pyridine nucleotides was measured simultaneously with the EEG activity from the surface of the brain cortex. From the data obtained, a few parameters were calcu...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490010205
更新日期：1975-01-01 00:00:00
abstract：:SH-SY5Y cells differentiate into neuronal-like cells and express marker proteins like growth-associated protein (GAP-43) and neuropeptide tyrosine when treated with a low concentration (16 nM) of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of growth factors or serum. Both control and d...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490410609
更新日期：1995-08-15 00:00:00
abstract：:Plexiform layer synaptic and photoreceptor cell components were investigated in retinas of Royal College of Surgeons (RCS) dystrophic rats transplanted with normal retinal pigment epithelial (RPE) cells by immunocytochemistry using previously characterized monoclonal antibodies. In retinas of normal adult rats and RPE...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360408
更新日期：1993-11-01 00:00:00
abstract：:Withdrawal of serum from cell cultures constitutes a useful model for the study of mechanisms involved in the regulation of Akt function in vitro. However, there have been several reports of changes in Akt activity that are not fully explained by the current model of phosphatidylinositol 3'-kinase (PI3K)/Akt signaling...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21516
更新日期：2008-02-15 00:00:00
abstract：:In the present study we report self-assembly of individual neurofilament (NF) triplet proteins (70K, 150K, and 200K daltons) isolated by anion exchange chromatography from bovine spinal cord. Formation of smooth 10-nm filaments by both NF 150K and NF 70K is shown. Optimal conditions for NK 150K filament formation were...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110204
更新日期：1984-01-01 00:00:00
abstract：:The twitcher mouse is well known as a naturally occurring authentic mouse model of human globoid cell leukodystrophy (GLD; Krabbe disease) due to genetic deficiency of lysosomal galactosylceramidase. The twitcher mice used most commonly are on the C57BL/6J background. We generated twitcher mice that were on the mixed ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20190
更新日期：2004-08-15 00:00:00
abstract：:We used the Plp1-overexpressing transgenic mouse model to investigate whether progressive demyelination of axons results in adaptive changes involving mitochondria within the axons. These models have myelinated axons from birth but gradually lose myelin and develop axonal loss associated with progressive neurological ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21867
更新日期：2009-02-01 00:00:00
abstract：:Immunocytochemical studies indicated that Purkinje cells cultured from chick embryonic cerebellum (embryonic day 8) strongly express a glutamate transporter EAAT2 cloned from human brain (GLT-1 in rat brain). At both 7 days and 14 days in culture, Purkinje neurons accumulated 1 microM [3H]L-glutamate via a potent "hig...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981201)54:5<595::AID-JNR
更新日期：1998-12-01 00:00:00
abstract：:The peroxisome proliferator-activated receptor-alpha (PPAR-alpha) plays a key role in lipid metabolism and inflammation. Recently, we demonstrated that administration of the PPAR-alpha agonists gemfibrozil and fenofibrate, inhibit the clinical signs of experimental autoimmune encephalomyelitis (EAE), the animal model ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20518
更新日期：2005-08-01 00:00:00
abstract：:The 14-3-3 proteins exist predominantly in the brain and may play regulatory roles in cellular processes of growth, differentiation, survival, and apoptosis. The biological functions, however, of the various 14-3-3 isoforms (beta, epsilon, eta, gamma, and zeta) in the brain remain unclear. We have reported previously ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20323
更新日期：2005-01-01 00:00:00
abstract：:Phencyclidine (PCP) is a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist. Several studies have demonstrated that chronic NMDA receptor antagonist treatment in humans and animals can cause long-term behavioral changes that are reminiscent of negative and cognitive schizophrenia-like symptoms. The muscari...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21247
更新日期：2007-05-01 00:00:00
abstract：:Perceptually similar stimuli, despite not being consciously distinguishable, may result in distinct cortical brain activations. Hypothesizing that perceptually similar tastes are discriminable by electroencephalography (EEG), we recorded 22 human participants' response to equally intense sweet-tasting stimuli: caloric...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24281
更新日期：2019-03-01 00:00:00
abstract：:Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system with an autoimmune attack on the components of the myelin sheath and axons. The etiology of the disease remains largely unknown, but it is commonly acknowledged that the development of MS probably results from the interacti...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24023
更新日期：2017-10-01 00:00:00
abstract：:Acoustic overstimulation causes apoptotic cell death in the cochlea. This death process is mediated, in part, by the mitochondrial signaling pathway involving Bcl-2 family proteins. Myeloid cell leukemia sequence 1 (Mcl-l) is an antiapoptotic member of the Bcl-2 family. Its involvement in noise-induced hair cell death...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22333
更新日期：2010-06-01 00:00:00
abstract：:Our previous study demonstrated that 2-phenylethynyl-butyltellurium (PEBT), an organotellurium compound, enhances memory in mice. In this study, the effects of PEBT on cognitive impairment induced by Aβ25-35 were assessed by Morris water maze and step-down inhibitory avoidance tasks. Mice received a single intracerebr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23211
更新日期：2013-06-01 00:00:00
abstract：:The growth and differentiation of neural precursor cells in the central nervous system (CNS) are regulated by their response to polypeptide growth factors which interact with specific transmembrane receptor tyrosine kinases (RTKs). We demonstrate that rat oligodendrocyte-type 2 astrocyte (O-2A) glial progenitor cells,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360512
更新日期：1993-12-01 00:00:00
abstract：:Alcohol-related events have been proposed to be under neurochemical control. For example, the concentration of methionine enkephalin (Met-enkephalin) in brain has been inversely correlated with ethanol consumption in alcohol-preferring and alcohol-nonpreferring mice. The concentrations of Met-enkephalin are controlled...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:α-Synuclein (α-syn) is the major component of pathological inclusions characteristic of several neurodegenerative disorders, such as Parkinson's disease. The major posttranslational modification of α-syn is phosphorylation at S129, and previous studies estimate that approximately 90% of α-syn in proteinaceous, patholo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22537
更新日期：2011-02-01 00:00:00
abstract：:We used cDNA arrays to investigate molecular aspects of the differentiation of an immortalized line of oligodendroglial progenitors, and of immunopan-purified primary cultures of oligodendroglial progenitors, to immature oligodendroglia. Developmental regulation of the proteolipid and 2-hydroxyacylsphingosine 1-galact...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000201)59:3<430::AID-JNR
更新日期：2000-02-01 00:00:00
abstract：:Cultures of dissociated hypothalamic cells taken from rat fetuses of 19 days of gestation were studied using time-lapse recording and sequential microphotography from 1 to 5 days in vitro (DIV) and at 7 and 21 DIV. Cultures were seeded with cells taken from fetuses grouped by sex or sexually mixed; experimental cultur...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330210
更新日期：1992-10-01 00:00:00
