The protective effects of naproxen against interleukin-1β (IL-1β)- induced damage in human umbilical vein endothelial cells (HUVECs).

abstract：:Affinity precipitation is a non-chromatographic method which is useful for purification and refolding of proteins. Quite often, a stimuli-sensitive polymer can be identified which selectively binds to the desired protein. For separation, the protein can be recovered from the precipitate of the protein-smart polymer co...

journal_title：Bioengineered

authors： Gautam S,Dubey P,Varadarajan R,Gupta MN

更新日期：2012-09-01 00:00:00

abstract：:Bacteriorhodopsin (BR), a model system in biotechnology, is a G-protein dependent trans membrane protein which serves as a light driven proton pump in the cell membrane of Halobacterium salinarum. Due to the linkage of retinal to the protein, it seems colored and has numbers of versatile properties. As in vitro cultur...

journal_title：Bioengineered

authors： Saeedi P,Moosaabadi JM,Sebtahmadi SS,Mehrabadi JF,Behmanesh M,Mekhilef S

更新日期：2012-11-01 00:00:00

abstract：:The properties of bacteriorhodopsin (BR) can be manipulated by genetic engineering. Therefore, by the methods of gene engineering, Asp85 was replaced individually by two other amino acids (D85V, D85S). The resulting recombinant proteins were assembled into soybean vesicles retinylated to form functional BR-like nano-p...

journal_title：Bioengineered

authors： Saeedi P,Moosaabadi JM,Sebtahmadi SS,Mehrabadi JF,Behmanesh M,Nejad HR,Nazaktabar A

更新日期：2012-09-01 00:00:00

abstract：:Therapeutic properties of recombinant proteins are very often affected by the composition and heterogeneity of their glycans. Conventional expression systems for recombinant pharmaceutical proteins typically do not address this problem and produce a mixture of glycoforms that are neither identical to human glycans nor...

journal_title：Bioengineered

authors： Strasser R

更新日期：2013-07-01 00:00:00

abstract：:In pursuit of better influenza vaccines, many strategies are being studied worldwide. An attractive alternative is the generation of a broadly cross-reactive vaccine based on the induction of cytotoxic T-lymphocytes (CTL) directed against conserved internal antigens of influenza A virus. The feasibility of this approa...

journal_title：Bioengineered

authors： Cargnelutti DE,Sánchez MV,Mattion NM,Scodeller EA

更新日期：2013-11-01 00:00:00

abstract：:To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endoglucanase I (EGI), nativ...

journal_title：Bioengineered

authors： Ribeiro O,Magalhães F,Aguiar TQ,Wiebe MG,Penttilä M,Domingues L

更新日期：2013-09-01 00:00:00

abstract：:Cancer is one of the most important health problems because many cases are difficult to prevent. Cancer still has unknown mechanisms of pathogenesis, and its capacity to produce temporary or permanent damage, besides death, is very high. Although many anticancer therapies are available, finding a cure for cancer conti...

journal_title：Bioengineered

authors： Pranchevicius MC,Vieira TR

更新日期：2013-09-01 00:00:00

abstract：:It has been documented that bacteria from the Burkholderia genera produce different kinds of compounds that inhibit plant pathogens, however in Burkholderia tropica, an endophytic diazotrophic and phosphate-solubilizing bacterium isolated from a wide diversity of plants, the capacity to produce antifungal compounds ha...

journal_title：Bioengineered

authors： Tenorio-Salgado S,Tinoco R,Vazquez-Duhalt R,Caballero-Mellado J,Perez-Rueda E

更新日期：2013-07-01 00:00:00

abstract：:In this study the growth of genetically modified Lactobacillus casei LAB6, overexpressing proline iminopeptidase PepI and its capacity to increase free proline was investigated during ripening of Edam cheese. The strain successfully survived 12 weeks of ripening period in cheese. The food-grade plasmid pLEB604, carryi...

journal_title：Bioengineered

authors： Navidghasemizad S,Takala TM,Alatossava T,Saris PE

更新日期：2013-11-01 00:00:00

abstract：:Research has shown that a greater variety of enzymes, as well as variety of microorganisms producing enzymes, can have an overall synergistic effect on the decomposition of lignocellulosic biomass for the production of value-added bio-products. Here, 8 cellulase-degrading bacterial isolates were selected to develop co...

journal_title：Bioengineered

authors： Maki M,Iskhakova S,Zhang T,Qin W

更新日期：2014-05-01 00:00:00

abstract：:Feline infectious peritonitis (FIP) is caused by feline coronaviruses (FCoVs) and represents one of the most important lethal infectious diseases of cats. To date, there is no efficacious prevention and treatment, and our limited knowledge on FIP pathogenesis is mainly based on analysis of experiments with field isola...

journal_title：Bioengineered

authors： Thiel V,Thiel HJ,Tekes G

更新日期：2014-01-01 00:00:00

abstract：:Maintaining high gene expression level during long-term culture is critical when producing therapeutic recombinant proteins using mammalian cells. Transcriptional silencing of promoters, most likely due to epigenetic events such as DNA methylation and histone modifications, is one of the major mechanisms causing produ...

journal_title：Bioengineered

authors： Mariati,Koh EY,Yeo JH,Ho SC,Yang Y

更新日期：2014-09-01 00:00:00

abstract：:Active recombinant proteins are used for studying the biological functions of genes and for the development of therapeutic drugs. Overexpression of recombinant proteins in bacteria often results in the formation of inclusion bodies, which are protein aggregates with non-native conformations. Protein refolding is an im...

journal_title：Bioengineered

authors： Yamaguchi H,Miyazaki M

更新日期：2015-01-01 00:00:00

abstract：:Three-dimensional (3D) tumor models generated in vitro using methods of tissue engineering are just starting to show potential for predictive studies of therapeutic targets and screening of anticancer drugs. By mimicking some of the key features of the in vivo tumor environment, these models allow us to grow physiolog...

journal_title：Bioengineered

authors： Villasante A,Vunjak-Novakovic G

更新日期：2015-01-01 00:00:00

abstract：:An NADH-dependent reductase (ClCR) was discovered by genome data mining. After ClCR was overexpressed in E. coli BL21, recombinant E. coli CCZU-T15 with high reductase activity and excellent stereoselectivity for the reduction of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (S)-4-chloro-3-hydroxybutanoate [(S)-CHBE...

journal_title：Bioengineered

authors： He YC,Zhang DP,Lu Y,Tao ZC,Ding Y,Wang LQ,Liu F

更新日期：2015-01-01 00:00:00

abstract：:In this paper, litchi freshness rapid non-destructive evaluating method using electronic nose (e-nose) and non-linear stochastic resonance (SR) was proposed. EN responses to litchi samples were continuously detected for 6 d Principal component analysis (PCA) and non-linear stochastic resonance (SR) methods were utiliz...

journal_title：Bioengineered

authors： Ying X,Liu W,Hui G

更新日期：2015-01-01 00:00:00

abstract：:The lantibiotics are a class of bacterially produced antimicrobial peptides (bacteriocins) that contain several unusual amino acids resulting from enzyme-mediated post-translational modifications. They exhibit high specific activity against Gram-positive targets, including many antibiotic-resistant pathogens, and cons...

journal_title：Bioengineered

authors： Field D,Cotter PD,Ross RP,Hill C

更新日期：2015-01-01 00:00:00

abstract：:The transcription factor NUCLEAR FACTOR Y (NF-Y) plays an essential role in many developmental and stress-responsive processes in plants. NF-Y composed of 3 subunits, NF-YA, NF-YB, and NF-YC, targets the CCAAT box, a common cis-element in eukaryotic promoters. We recently identified a gene TaNF-YA10-1 from the wheat s...

journal_title：Bioengineered

authors： Ma X,Li C,Wang M

更新日期：2015-01-01 00:00:00

abstract：:In this paper, a novel benzene quantitative analysis method utilizing miniaturized metal ionization gas sensor and non-linear bistable dynamic system was investigated. Al plate anodic gas-ionization sensor was installed for electrical current-voltage data measurement. Measurement data was analyzed by non-linear bistab...

journal_title：Bioengineered

authors： Tang X,Liu F

更新日期：2015-01-01 00:00:00

abstract：:The scientific evidence supporting the gut microbiome in relation to health maintenance and links with various disease states afflicting humans, from metabolic to mental health, has grown dramatically in the last few years. Strategies addressing the positive modulation of microbiome functionality associated with these...

journal_title：Bioengineered

authors： Linares DM,Ross P,Stanton C

更新日期：2016-01-01 00:00:00

abstract：:Malaria remains a major global health problem. Parasite resistance to existing drugs makes development of new antimalarials an urgency. The protein synthesis machinery is an excellent target for the development of new anti-infectives, and aminoacyl-tRNA synthetases (aaRS) have been validated as antimalarial drug targe...

journal_title：Bioengineered

authors： Saint-Léger A,Sinadinos C,Ribas de Pouplana L

更新日期：2016-04-02 00:00:00

abstract：:This paper presents a new ChainMail method for real-time soft tissue simulation. This method enables the use of different material properties for chain elements to accommodate various materials. Based on the ChainMail bounding region, a new time-saving scheme is developed to improve computational efficiency for isotro...

journal_title：Bioengineered

authors： Zhang J,Zhong Y,Smith J,Gu C

更新日期：2016-07-03 00:00:00

abstract：:In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro. In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, deter...

journal_title：Bioengineered

authors： Wang XH,Wang Z,Zhang J,Qi HX,Chen J,Xu M

更新日期：2016-09-02 00:00:00

abstract：:2-Phenylethanol (2-PE) is a high value aromatic alcohol with a rose-like odor that is utilized in the cosmetics and other industries. Although the chemical routes of 2-PE production have been altered by some microbial transformation processes, the poor tolerance to organic solvents of these microorganisms has limited ...

journal_title：Bioengineered

authors： Lu X,Wang Y,Zong H,Ji H,Zhuge B,Dong Z

更新日期：2016-11-01 00:00:00

abstract：:There is a growing interest in applying tobacco agroinfiltration for recombinant protein production in a plant based system. However, in such a system, the action of proteases might compromise recombinant protein production. Protease sensitivity of model recombinant foot-and-mouth disease (FMD) virus P1-polyprotein (P...

journal_title：Bioengineered

authors： Pillay P,Kunert KJ,van Wyk S,Makgopa ME,Cullis CA,Vorster BJ

更新日期：2016-11-01 00:00:00

abstract：:ABSTACT The purpose of this study is to research the morphologies and functional characteristics of the cell-scaffold complex in vitro constructed under dynamic culture conditions. BMSCs were isolated from the long bones of Fischer344 rats, and performed in vitro amplification to the third generation as seed cells, to...

journal_title：Bioengineered

authors： Huang L,Xu F,Guo B,Ma J,Zhao J

更新日期：2016-07-03 00:00:00

abstract：:The western area of the Jilin province, a typical seasonal frost region, is located in the southern Songnen plain of China. Significantly salinized soils are widely distributed on the Songnen plain in western Jilin. Soil salinization can cause degradation of cultivated land and grass, which threatens the human environ...

journal_title：Bioengineered

authors： Bao S,Wang Q,Bao X,Li M,Wang Z

更新日期：2016-09-02 00:00:00

abstract：:RNA interference (RNAi) technology is considered as an alternative for control of pests. However, RNAi has not been used in field conditions yet, since delivering exogenous ds/siRNA to target pests is very difficult. The laboratory methods of introducing the ds/siRNA into insects through feeding, micro feeding / dripp...

journal_title：Bioengineered

authors： Hu Q,Wu W

更新日期：2016-11-01 00:00:00

abstract：:The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that use this genus have been developed. Amycolatopsis mediterranei is an industrial-scale pr...

journal_title：Bioengineered

authors： Li C,Zhou L,Wang Y,Zhao G,Ding X

更新日期：2017-09-03 00:00:00

abstract：:Petroleum wastewater samples from oil refinery and oil exploration site were treated by hyper phenol-tolerant Bacillus cereus (AKG1 and AKG2) in laboratory-scale batch process to assess their bioremediation efficacy. Quality of the treated wastewater samples were analyzed in terms of removal of chemical oxygen demand ...

journal_title：Bioengineered

authors： Banerjee A,Ghoshal AK

更新日期：2017-09-03 00:00:00