Physiological oxygen tension is relevant to MHC-1 expression, spontaneous transformation, and interferon response of in vitro aging murine fibroblasts.

Abstract:

:Working from the hypothesis that modest deviations from physiological oxygen tension will influence cell characteristics important for infections/immunity and tumor development, cells were studied at three oxygen tensions during in vitro aging. Primary mouse embryo fibroblasts were established and subsequently passaged at 3, 6, and 18 kPa oxygen tension (6 representing normal tissue tension and 18 being the conventionally tension at in vitro cultures). The growth rate was slightly higher at 6 than 3 and 18 kPa. All cultures eventually stopped growing and subsequently transformed to nonmalignant cells with unlimited growth capacity. Cells kept at 3 kPa reached the highest number of cell doublings before crisis. Stimulation with PolyI:C resulted in detectable interferon response only at the high oxygen tension, and after transformation none of the cultures responded with interferon production. Expression of the major histocompatibility complex H-2K was elevated above and below physiological oxygen tension, indicating regulatory processes optimizing MHC expression at about physiological oxygen tension.

journal_name

Exp Gerontol

journal_title

Experimental gerontology

authors

Ebbesen P,Hager H,Aboagye-Mathiesen G,Petersen PM,Lützhøft J,Villadsen JA,Zdravkovic M,Dalsgaard AM,Zachar V

doi

10.1016/0531-5565(93)90046-g

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

573-8

issue

6

eissn

0531-5565

issn

1873-6815

journal_volume

28

pub_type

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