Abstract:
:Working from the hypothesis that modest deviations from physiological oxygen tension will influence cell characteristics important for infections/immunity and tumor development, cells were studied at three oxygen tensions during in vitro aging. Primary mouse embryo fibroblasts were established and subsequently passaged at 3, 6, and 18 kPa oxygen tension (6 representing normal tissue tension and 18 being the conventionally tension at in vitro cultures). The growth rate was slightly higher at 6 than 3 and 18 kPa. All cultures eventually stopped growing and subsequently transformed to nonmalignant cells with unlimited growth capacity. Cells kept at 3 kPa reached the highest number of cell doublings before crisis. Stimulation with PolyI:C resulted in detectable interferon response only at the high oxygen tension, and after transformation none of the cultures responded with interferon production. Expression of the major histocompatibility complex H-2K was elevated above and below physiological oxygen tension, indicating regulatory processes optimizing MHC expression at about physiological oxygen tension.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Ebbesen P,Hager H,Aboagye-Mathiesen G,Petersen PM,Lützhøft J,Villadsen JA,Zdravkovic M,Dalsgaard AM,Zachar Vdoi
10.1016/0531-5565(93)90046-gsubject
Has Abstractpub_date
1993-11-01 00:00:00pages
573-8issue
6eissn
0531-5565issn
1873-6815journal_volume
28pub_type
杂志文章abstract::The cold-induced enhancement of non-shivering thermogenesis (NST), involving brown-adipose tissue (BAT) metabolism, could participate to impair energy balance in the aged gray mouse lemur (Microcebus murinus). We first investigated the age-related modulations of cold-stimulated BAT cell morphology and contents. Then, ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2010.03.013
更新日期:2010-06-01 00:00:00
abstract::DNA methylation patterns change as individuals grow older, and DNA methylation appears susceptible to modification by the diet. Thus DNA methylation may be a mechanism through which diet can affect aging and longevity. We propose that effects on DNA methylation also contribute to the extension in lifespan observed in ...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2012.07.010
更新日期:2013-07-01 00:00:00
abstract::In this study we report the identification of FAH domain containing protein 1 (FAHD1), a recently described member of the fumarylacetoacetate hydrolase (FAH) superfamily of metabolic enzymes, as a novel player in the regulation of cellular senescence. FAHD1 was found in a proteomic screen searching for mitochondrial p...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.03.004
更新日期:2017-06-01 00:00:00
abstract:INTRODUCTION:The ageing process can be influenced by different dietary patterns and dietary factors. AIM:To analyse the association of dietary factors, which include the Mediterranean diet adherence, the compliance with the "Sociedad Española de Nutrición Comunitaria" food portion recommendations, and the intake of di...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.01.019
更新日期:2019-05-01 00:00:00
abstract::Several studies have shown that centenarians have better cardiovascular risk profiles compared to younger old people. Some reports have revealed that cardiovascular diseases (i.e. hypertension, diabetes, angina and/or myocardial infarction) are less common in centenarians respect to 70 and 80 years old persons. In ord...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2007.06.009
更新日期:2008-02-01 00:00:00
abstract::Mitochondrial proton leak may modulate reactive oxygen species (ROS) production and play a role in aging. The purpose of this study was to determine proton leak across the life span in skeletal mitochondria from calorie-restricted and UCP2/3 overexpressing mice. Proton leak in isolated mitochondria and markers of oxid...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2008.09.010
更新日期:2008-12-01 00:00:00
abstract::Aging is associated with a reduction in the maximum density of n-methyl-d-aspartate (NMDA)-sensitive glutamate binding sites in the hippocampus of Fischer 344 rats. This study was designed to investigate the effect of acetyl-l-carnitine (ALCAR) on NMDA receptors in the old rat (24 months) after chronic or single-dose ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(93)90042-c
更新日期:1993-11-01 00:00:00
abstract::The goal of these experiments was to determine if the increased longevity characteristics of our genetically selected long lived line of Drosophila could be attributed to metabolic differences. The data shows an inverse relationship between life span and temperature for both the long lived (L) and normal (R) strains; ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(88)90020-4
更新日期:1988-01-01 00:00:00
abstract::During the course of life, cyclic females face a state of midlife transition that occurs in a fully functioning neurological system, and results in reproductive senescence. The authors' hypothesis was that changes in the activity noradrenergic neurons may be one of the factors involved in this phenomenon. The aim of t...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.04.015
更新日期:2016-08-01 00:00:00
abstract::Skeletal remodelling is a continuous process during life and is still active also in extreme senescence. In the elderly, bone resorption often prevails over bone formation, causing bone loss and fragility. Elderly subjects are exposed to the risk of fractures, and loss of self-sufficiency, if considering that the prox...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2007.06.013
更新日期:2008-02-01 00:00:00
abstract::Long-term caloric restriction (CR) has been shown to extend maximum life span in laboratory rodents. We investigated the activities of gluconeogenic and transaminase enzymes in the livers of old and young mice fed either control or calorie-restricted diets. Livers were sampled 48 h after the last scheduled feeding tim...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(02)00202-4
更新日期:2003-03-01 00:00:00
abstract::Synaptic disturbances may play a key role in the pathophysiology of Alzheimer's disease. To characterize differential synaptic alterations in the brains of Alzheimer patients, chromogranin A, chromogranin B and secretoneurin were applied as soluble constituents for large dense core vesicles, synaptophysin as a vesicle...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2003.09.018
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:Depression is common in hemodialysis patients. Reduced DHEA-S levels have been shown to be associated with depression in general population. Abnormalities in hormone production and metabolism are found in hemodialysis patients. However, the association between DHEA-S levels and depression in hemodialysis pati...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2009.08.009
更新日期:2009-11-01 00:00:00
abstract:OBJECTIVE:Aging has been reported to be associated with changes in immune function. Although frequent infection and the development of malignancy suggest the decline of immune function with aging, changes toward proinflammatory conditions also develop at the same time. Th17 cells are well known CD4(+) T cell subpopulat...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2013.10.006
更新日期:2014-01-01 00:00:00
abstract::Histological surveys of the hearts of guppies Poecilia reticulatus throughout their lifespan showed no aging changes until the fish were three years old. In younger fishes the hearts were healthy, even in three individuals with "wasting" disease, where there was considerable loss of muscle throughout the body. There w...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(84)90048-2
更新日期:1984-01-01 00:00:00
abstract::Chronic low-grade elevations in circulating levels of interleukin (IL)-6 act as a marker of subclinical cardiovascular diseases (CVD) and provide an independent predictor of increased mortality in elderly populations. The purpose of the present study was to test the hypothesis that the IL-6 -174G>C promoter polymorphi...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2003.10.012
更新日期:2004-02-01 00:00:00
abstract::Sex hormones are known to help maintaining the cognitive ability in male and female rats. Hypogonadism results in the reduction of the dendritic spines of central neurons which is believed to undermine memory and cognition and cause fatigue and poor concentration. In our previous studies, we have reported age-related ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2014.06.010
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:1) To determine if there was a practice effect associated with walk tests performed by frail older adults with and without dementia, 2) to examine the role of systematic cueing in the walk tests for those with dementia, and 3) to make recommendations to testing protocols of the walk tests for frail older adu...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.110648
更新日期:2019-09-01 00:00:00
abstract::To understand the mechanism of aging at the cellular level, cellular senescence has been extensively studied as an experimental model of aging in vitro. Although several hypotheses have been proposed for the mechanism of cellular senescence, none of them could give a comprehensive framework to the mechanism. In this s...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(98)00008-4
更新日期:1998-08-01 00:00:00
abstract::The loss of bone mineral with aging and subsequent development of osteoporosis is a common problem in elderly women, and as life expectancy increases, in elderly men as well. Space flight also causes bone loss and could be a limiting factor for long duration missions, such as, a Mars expedition or extended occupation ...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/0531-5565(91)90011-a
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Longitudinal studies on sleep duration and grip strength decline are limited. This study aimed to evaluate the associations of baseline sleep duration with follow-up grip strength and grip strength changeover time among a large sample of middle-aged and older Chinese. METHODS:Data from China Health and Reti...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2018.01.009
更新日期:2018-03-01 00:00:00
abstract::Aging is associated with an increased incidence of heart failure, but the existence of an age-related cardiomyopathy remains controversial. Differences in strain, age and technique of measuring cardiac function differ between experiments, confounding the interpretation of these studies. Additionally, the structural an...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2011.02.010
更新日期:2011-07-01 00:00:00
abstract::The CG9940 gene, which encodes the NAD(+) synthase protein in Drosophila, is conserved in human, zebra fish, and mosquito. NAD(+) synthase is a homodimer, which catalyzes the final step in de novo nicotinamide adenine dinucleotide (NAD(+)) biosynthesis, an amide transfer from either ammonia or glutamine to nicotinic a...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.07.006
更新日期:2016-10-01 00:00:00
abstract::While it may be possible to employ panels of molecular parameters which correlate with senescence in vivo or in vitro, in a manner analogous to the use of mutagenesis assays for economic carcinogen screening, such an endeavor would at present be impeded by the absence of a clear mechanistic rationale for focusing on p...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/0531-5565(88)90030-7
更新日期:1988-01-01 00:00:00
abstract::Gene expression is much altered in aging. We observed age-dependent decline of core clock genes' expression in the whole body of the fruit fly. We hypothesized that inducible overexpression of clock genes (cry, per, tim, cyc and Clk) in the nervous system can improve healthspan of D. melanogaster. We studied the lifes...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2018.11.005
更新日期:2019-03-01 00:00:00
abstract::A growing body of evidence indicates that dysregulation of cerebral biometals (Fe, Cu, Zn) and their interactions with APP and Abeta amyloid may contribute to the Alzheimer's amyloid pathology, and thus metal chelation could be a rational therapeutic approach for interdicting AD pathogenesis. However, poor target spec...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.08.016
更新日期:2004-11-01 00:00:00
abstract::Post-translational oxidative protein modifications which are more marked during aging and/or high-calorie (HC) diets affect protein function and metabolism. Protein function and metabolism are different according to the type of muscle proteins. Oxidative muscle protein modifications may thus be associated with age-rel...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2011.11.007
更新日期:2012-02-01 00:00:00
abstract::Diabetic peripheral neuropathy (DPN) is associated with peripheral arterial disease and endothelial dysfunction. We investigated the effect of exercise training on the measures of superficial femoral artery (SFA) and neuropathic symptoms in patients with DPN. In a randomized-controlled trial, 31 volunteers with establ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.110799
更新日期:2020-03-01 00:00:00
abstract::A longitudinal study was performed to confirm the inverse relationship between in vivo age and in vitro proliferative capacity previously observed in a cross-sectional study, and to investigate the relationship between the growth of dermal fibroblasts in vitro and a physiological function (i.e., wound repair) that is ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(91)90058-t
更新日期:1991-01-01 00:00:00
abstract::Aging is associated with increased cellular levels of lipid peroxides and reactive oxygen species. This increase in the radical species may affect membrane fluidity and consequently membrane functions. We attempted to determine whether age-related increases in lipid peroxide and reactive oxygen species levels affect t...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(01)00164-4
更新日期:2001-12-01 00:00:00