Abstract:
:A rapid and simple in vitro method is described which measures the extent of unrecoiled solids compression when a complex biopolymer is subjected to a centrifugal force. This method, termed the compaction assay, was used to assess the response of purulent cystic fibrosis (CF) sputum samples to the addition of recombinant human deoxyribonuclease I (rhDNase). Enzyme treatment resulted in a dramatic decrease in DNA size, a redistribution of total DNA content from the pellet to supernatant, a significant decrease in that pellet volume and a decrease in elastic modulus. Sample elasticity, measured by a dynamic cone and plate viscometer, could be related to compaction assay results. These results suggest that the compaction assay may be a useful in vitro method for rapidly assessing the actions of enzymatic disruption of a complex biopolymer, such as that observed for the actions of rhDNase on purulent airway secretions.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Daugherty AL,Patapoff TW,Clark RC,Sinicropi DV,Mrsny RJdoi
10.1016/0142-9612(95)91129-msubject
Has Abstractpub_date
1995-05-01 00:00:00pages
553-8issue
7eissn
0142-9612issn
1878-5905pii
014296129591129Mjournal_volume
16pub_type
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