Compaction assay: a rapid and simple in vitro method to assess the responsiveness of a biopolymer matrix to enzymatic modification.

Abstract:

:A rapid and simple in vitro method is described which measures the extent of unrecoiled solids compression when a complex biopolymer is subjected to a centrifugal force. This method, termed the compaction assay, was used to assess the response of purulent cystic fibrosis (CF) sputum samples to the addition of recombinant human deoxyribonuclease I (rhDNase). Enzyme treatment resulted in a dramatic decrease in DNA size, a redistribution of total DNA content from the pellet to supernatant, a significant decrease in that pellet volume and a decrease in elastic modulus. Sample elasticity, measured by a dynamic cone and plate viscometer, could be related to compaction assay results. These results suggest that the compaction assay may be a useful in vitro method for rapidly assessing the actions of enzymatic disruption of a complex biopolymer, such as that observed for the actions of rhDNase on purulent airway secretions.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Daugherty AL,Patapoff TW,Clark RC,Sinicropi DV,Mrsny RJ

doi

10.1016/0142-9612(95)91129-m

subject

Has Abstract

pub_date

1995-05-01 00:00:00

pages

553-8

issue

7

eissn

0142-9612

issn

1878-5905

pii

014296129591129M

journal_volume

16

pub_type

杂志文章
  • Processing and mechanical properties of HA/UHMWPE nanocomposites.

    abstract::A hydroxyapatite (HA) particulate reinforced ultrahigh molecular weight polyethylene (UHMWPE) nanocomposite was fabricated by compounding HA and UHMWPE mixtures in paraffin oil using twin-screw extrusion and then compression molding. Scanning electron microscope images revealed that HA aggregates were broken down to n...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2006.02.023

    authors: Fang L,Leng Y,Gao P

    更新日期:2006-07-01 00:00:00

  • Crosslinked type II collagen matrices: preparation, characterization, and potential for cartilage engineering.

    abstract::The limited intrinsic repair capacity of articular cartilage has stimulated continuing efforts to develop tissue engineered analogues. Matrices composed of type II collagen and chondroitin sulfate (CS), the major constituents of hyaline cartilage, may create an appropriate environment for the generation of cartilage-l...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(02)00067-4

    authors: Pieper JS,van der Kraan PM,Hafmans T,Kamp J,Buma P,van Susante JL,van den Berg WB,Veerkamp JH,van Kuppevelt TH

    更新日期:2002-08-01 00:00:00

  • Magnetic nanoparticles encapsulated into biodegradable microparticles steered with an upgraded magnetic resonance imaging system for tumor chemoembolization.

    abstract::In this work, therapeutic magnetic micro carriers (TMMC) guided in real time by a magnetic resonance imaging (MRI) system are proposed as a mean to improve drug delivery to tumor sites. MRI steering constraints and physiological parameters for the chemoembolization of liver tumors were taken into account to design mag...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.08.005

    authors: Pouponneau P,Leroux JC,Martel S

    更新日期:2009-10-01 00:00:00

  • Human iPSC-derived blood-brain barrier microvessels: validation of barrier function and endothelial cell behavior.

    abstract::Microvessels of the blood-brain barrier (BBB) regulate transport into the brain. The highly specialized brain microvascular endothelial cells, a major component of the BBB, express tight junctions and efflux transporters which regulate paracellular and transcellular permeability. However, most existing models of BBB m...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2018.10.023

    authors: Linville RM,DeStefano JG,Sklar MB,Xu Z,Farrell AM,Bogorad MI,Chu C,Walczak P,Cheng L,Mahairaki V,Whartenby KA,Calabresi PA,Searson PC

    更新日期:2019-01-01 00:00:00

  • Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype.

    abstract::Valvular interstitial cells (VICs) actively maintain and repair heart valve tissue; however, persistent activation of VICs to a myofibroblast phenotype can lead to aortic stenosis. To better understand and quantify how microenvironmental cues influence VIC phenotype and myofibroblast activation, we compared expression...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.09.035

    authors: Mabry KM,Payne SZ,Anseth KS

    更新日期:2016-01-01 00:00:00

  • The development of acid-base reaction cements as formulations for the controlled release of trace elements.

    abstract::Novel acid-base reaction cements have been developed for use as controlled release formulations. Many new ones have been discovered and assessed; these include those which are capable of releasing copper, cobalt or selenium singly or in combination. A selection was made of the most suitable formulations for this purpo...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(86)90065-7

    authors: Prosser HJ,Wilson AD,Groffman DM,Brookman PJ,Allen WM,Gleed PT,Manston R,Sansom BF

    更新日期:1986-03-01 00:00:00

  • Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells.

    abstract::Expansion of hematopoietic stem cells (HSCs) from cord blood is highly desired for treatment and transplantation of adult patients for hematologic diseases. For efficient proliferation of HSCs, CD34(+) cells from cord blood were co-cultured with microencapsulated murine stromal cells (HESS-5) or immortalized human mes...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2007.07.033

    authors: Fujimoto N,Fujita S,Tsuji T,Toguchida J,Ida K,Suginami H,Iwata H

    更新日期:2007-11-01 00:00:00

  • Interactions between meniscal cells and a self assembled biomimetic surface composed of hyaluronic acid, chitosan and meniscal extracellular matrix molecules.

    abstract::Menisci are one of the most commonly injured parts of the knee with a limited healing potential. This study focuses on fabrication and characterization of biomimetic surfaces for meniscal tissue engineering. To achieve this, a combination of hyaluronic acid/chitosan (HA/CH) mutilayers with covalently immobilized major...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.04.018

    authors: Tan GK,Dinnes DL,Butler LN,Cooper-White JJ

    更新日期:2010-08-01 00:00:00

  • Hierarchically constructed selenium-doped bone-mimetic nanoparticles promote ROS-mediated autophagy and apoptosis for bone tumor inhibition.

    abstract::Biomimetic materials are often capable of subtly affecting tissue development, regeneration and carcinogenesis due to their high similarity to natural tissues. Despite the benefit of using such materials in tissue engineering, their prospective use in cancer therapy has been neglected, particularly the functions and m...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2020.120253

    authors: Li X,Wang Y,Chen Y,Zhou P,Wei K,Wang H,Wang J,Fang H,Zhang S

    更新日期:2020-10-01 00:00:00

  • Effect of pH on protein adsorption to hydroxyapatite and tricalcium phosphate ceramics.

    abstract::Calcium phosphate ceramics used for clinical applications vary considerably in their phase composition. A range of hydroxyapatite and tricalcium phosphate powders, some of which are used in plasma-sprayed coatings for metal prostheses, were found to leach substances which change the pH of water and cell culture medium...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(96)00157-3

    authors: Sharpe JR,Sammons RL,Marquis PM

    更新日期:1997-03-01 00:00:00

  • Design maps for cellular uptake of gene nanovectors by computer simulation.

    abstract::Understanding how nanovectors transport DNA molecules through cell membranes is of great importance in gene therapy. In this paper, we systematically investigate the mechanism of cellular uptake of cationic polymeric nanovectors containing DNA molecules through dissipative particle dynamics simulations. Our results sh...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2013.06.067

    authors: Ding HM,Ma YQ

    更新日期:2013-11-01 00:00:00

  • The significance of pore microarchitecture in a multi-layered elastomeric scaffold for contractile cardiac muscle constructs.

    abstract::Multi-layered poly(glycerol-sebacate) (PGS) scaffolds with controlled pore microarchitectures were fabricated, combined with heart cells, and cultured with perfusion to engineer contractile cardiac muscle constructs. First, one-layered (1L) scaffolds with accordion-like honeycomb shaped pores and elastomeric mechanica...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.11.032

    authors: Park H,Larson BL,Guillemette MD,Jain SR,Hua C,Engelmayr GC Jr,Freed LE

    更新日期:2011-03-01 00:00:00

  • Microtopographical features generated by photopolymerization recruit RhoA/ROCK through TRPV1 to direct cell and neurite growth.

    abstract::Cell processes, including growth cones, respond to biophysical cues in their microenvironment to establish functional tissue architecture and intercellular networks. The mechanisms by which cells sense and translate biophysical cues into directed growth are unknown. We used photopolymerization to fabricate methacrylat...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.02.057

    authors: Li S,Tuft BW,Xu L,Polacco MA,Clarke JC,Guymon CA,Hansen MR

    更新日期:2015-06-01 00:00:00

  • Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation.

    abstract::Hydrogels produced from self-assembling peptides and peptide derivatives are being investigated as synthetic extracellular matrices for defined cell culture substrates and scaffolds for regenerative medicine. In many cases, however, they are less stiff than the tissues and extracellular matrices they are intended to m...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2008.01.008

    authors: Jung JP,Jones JL,Cronier SA,Collier JH

    更新日期:2008-05-01 00:00:00

  • Combination strategy of multi-layered surface camouflage using hyperbranched polyethylene glycol and immunosuppressive drugs for the prevention of immune reactions against transplanted porcine islets.

    abstract::This study suggests a novel method of stabilizing fragile porcine islets to prevent the dissociation after isolation and reducing immune cell invasion in a combination therapy of 'surface camouflaging' and immunosuppressive drugs (FK506, Rapamycin, MR-1, anti-CD19 mAb, and Clodrosome(®)) to effectively alleviate overa...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2016.01.039

    authors: Haque MR,Jeong JH,Byun Y

    更新日期:2016-04-01 00:00:00

  • Importance of hydroxyapatite particles characteristics on cytokines production by human monocytes in vitro.

    abstract::Calcium phosphate bioceramics have been applied as bone substitutes for several decades. Aseptic loosening after total joint arthroplasty is a major problem in orthopaedic surgery. Hydroxyapatite particles from materials wear have been reported as the main cause of implant failure. For this reason, an investigation in...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(03)00089-9

    authors: Laquerriere P,Grandjean-Laquerriere A,Jallot E,Balossier G,Frayssinet P,Guenounou M

    更新日期:2003-07-01 00:00:00

  • The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching.

    abstract::Vascular endothelial growth factor (VEGF) has been extensively investigated to promote vascularization at damaged or diseased sites and in tissue implants. Here we are interested in determining if the manner in which VEGF is presented from a scaffold to endothelial cells influences the architecture of the blood vessel...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.06.027

    authors: Anderson SM,Siegman SN,Segura T

    更新日期:2011-10-01 00:00:00

  • Analysis of surface cleanliness of three commercial dental implants.

    abstract::Six routinely packaged commercially pure titanium dental implants (three commercial brands) were analysed using secondary ion mass spectrometry to examine the outermost surface layer. The aim of the analysis was to compare the surface purity (99.95%) of the implants with the substrate metal, common to all three brands...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(92)90017-i

    authors: Edwards BN,Gold BR

    更新日期:1992-01-01 00:00:00

  • Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating.

    abstract::We report a new template-directed method for the fabrication of hydroxyapatite (HAp) sponges by using amino-acid-coated HAp nanoparticles dispersed within a viscous polysaccharide (dextran sulfate) matrix, and describe the use of these materials for the viability and proliferation of human bone marrow stromal cells. T...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2005.04.037

    authors: Gonzalez-McQuire R,Green D,Walsh D,Hall S,Chane-Ching JY,Oreffo RO,Mann S

    更新日期:2005-11-01 00:00:00

  • In vitro assessment of bacterial adhesion to Hydromer-coated cerebrospinal fluid shunts.

    abstract::The adherence of five strains of Staphylococcus epidermidis and one strain of S. aureus to both untreated and Hydromer-coated silicone rubber cerebrospinal fluid shunts was studied in vitro using epifluorescent image analysis. All five strains of S. epidermidis showed similar levels of adherence to untreated shunts, w...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(93)90021-s

    authors: Bridgett MJ,Davies MC,Denyer SP,Eldridge PR

    更新日期:1993-02-01 00:00:00

  • Folic acid conjugated nanoparticles of mixed lipid monolayer shell and biodegradable polymer core for targeted delivery of Docetaxel.

    abstract::A system of nanoparticles of mixed lipid monolayer shell and biodegradable polymer core was developed for targeted delivery of anticancer drugs with Docetaxel as a model drug, which provide targeting versatility with a quantitative control of the targeting effect by adjusting the lipid component ratio of the mixed lip...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.09.036

    authors: Liu Y,Li K,Pan J,Liu B,Feng SS

    更新日期:2010-01-01 00:00:00

  • The effect of poly (ethylene-co-vinyl alcohol) on senescence-associated alterations of human dermal fibroblasts.

    abstract::It is well known that biomaterials play an important role in the regulation of adhesion and growth of a variety of cultured cell types. However, whether biomaterials are associated with the senescence of cultured cells is not known. The present work shows that the decrease of the hydrophobic property of poly (ethylene...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.11.048

    authors: Lou PJ,Chiu MY,Chou CC,Liao BW,Young TH

    更新日期:2010-03-01 00:00:00

  • Development of highly reactive mono-(meth)acrylates as reactive diluents for dimethacrylate-based dental resin systems.

    abstract::Reactive diluents such as triethyleneglycol-dimethacrylate (TEGDMA) have been widely used with bisphenol-A-glycidyl-dimethacrylate (Bis-GMA) to achieve restorative resins with appropriate viscosity and higher conversion. However, additional water sorption and polymerization shrinkage were also introduced. The aim of t...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2004.04.041

    authors: Lu H,Stansbury JW,Nie J,Berchtold KA,Bowman CN

    更新日期:2005-04-01 00:00:00

  • SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics.

    abstract::Surface plasmon resonance (SPR) sensing has long been used to study biomolecular binding events and their kinetics in a label-free way. This approach has recently been extended to SPR microscopy, which is an ideal tool for probing large microarrays of biomolecules for their binding interactions with various partners a...

    journal_title:Biomaterials

    pub_type: 杂志文章,评审

    doi:10.1016/j.biomaterials.2007.01.047

    authors: Campbell CT,Kim G

    更新日期:2007-05-01 00:00:00

  • Intracellular redox-activated anticancer drug delivery by functionalized hollow mesoporous silica nanoreservoirs with tumor specificity.

    abstract::In this study, a type of intracellular redox-triggered hollow mesoporous silica nanoreservoirs (HMSNs) with tumor specificity was developed in order to deliver anticancer drug (i.e., doxorubicin (DOX)) to the target tumor cells with high therapeutic efficiency and reduced side effects. Firstly, adamantanamine was graf...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2014.05.058

    authors: Luo Z,Hu Y,Cai K,Ding X,Zhang Q,Li M,Ma X,Zhang B,Zeng Y,Li P,Li J,Liu J,Zhao Y

    更新日期:2014-09-01 00:00:00

  • HEMA/MMMA microcapsule implants in hemiparkinsonian rat brain: biocompatibility assessment using [3H]PK11195 as a marker for gliosis.

    abstract::Microencapsulation of dopamine-secreting cells in biocompatible, semi-permeable polymer membranes has been proposed as an alternative strategy for dopamine replacement for Parkinson's disease. In order to assess the viability of this proposal, dopamine-secreting PC12 cells were immunoisolated via microencapsulation in...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(97)00241-x

    authors: Campioni EG,Nobrega JN,Sefton MV

    更新日期:1998-04-01 00:00:00

  • The influence of the restoration-tooth interface in light cured composite restorations: a finite element analysis.

    abstract::This study examines the early shrinkage behaviour of dental composite resins, and in particular the interfacial stresses around the margins of a composite restoration. The development of stresses at the restoration-tooth interface can have a detrimental effect on the longevity of a restoration. The influence of this i...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(01)00058-8

    authors: Ensaff H,O'Doherty DM,Jacobsen PH

    更新日期:2001-12-01 00:00:00

  • Preparation of three-dimensional interconnected macroporous cellulosic hydrogels for soft tissue engineering.

    abstract::This work exploits the thermal responsive phase behavior of hydroxypropylcellulose to produce 3D interconnected macroporous hydrogels in aqueous environment. Hydroxypropylcellulose was modified with allyl isocyanate, and their temperature mediated phase behavior was studied as a function of degree of modification (DS)...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.07.059

    authors: Yue Z,Wen F,Gao S,Ang MY,Pallathadka PK,Liu L,Yu H

    更新日期:2010-11-01 00:00:00

  • Preparation of bioactive Ti6Al4V surfaces by a simple method.

    abstract::Boiling diluted alkali incubation was found to be an effective way to prepare bioactive Ti6Al4V surfaces, whether polished or not, as indicated in vitro after immersion in two different supersaturated calcification solutions (SCSs). The induction of calcium phosphate (Ca-P) precipitation from the SCSs is most probably...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(97)00232-9

    authors: Wen HB,de Wijn JR,Cui FZ,de Groot K

    更新日期:1998-01-01 00:00:00

  • Skin permeating nanogel for the cutaneous co-delivery of two anti-inflammatory drugs.

    abstract::The aim of this study was to develop an effective drug delivery system for the simultaneous topical delivery of two anti-inflammatory drugs, spantide II (SP) and ketoprofen (KP). To achieve this primary goal, we have developed a skin permeating nanogel system (SPN) containing surface modified polymeric bilayered nanop...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.11.011

    authors: Shah PP,Desai PR,Patel AR,Singh MS

    更新日期:2012-02-01 00:00:00