Abstract:
:Malondialdehyde (MDA) is a stable produce of arachidonic acid metabolism, catalyzed by the enzyme cyclo-oxygenase. The experimental conditions for measuring the kinetics of MDA formation in rat citrated platelet-rich plasma were defined. Platelets were stimulated with either arachidonic acid, the substrate of MDA, or thrombin, an enzyme which induces release of free arachidonic acid from platelet membrane phospholipids. MDA formation was almost linear for a limited period of time (between 0 and 2 min with arachidonic acid and between 1 and 3 min with thrombin) and was concentration-dependent with saturation kinetics. The hyperbolic curves obtained could be recast in linear plots (according to Woolf transformation S/V versus S) when arachidonic acid was used. With thrombin, in contrast, the highest concentration at which no MDA production could be detected (3 NIH u/ml) had to be subtracted from each concentration of the enzyme used to obtain Woolf plots. The apparent Km value of arachidonic acid was 0.49 /+- 0.09 mM and Vmax was 1.44 /+- 0.06 nmoles MDA/1.4 Z 10(9) platelets/min. The corresponding values in experiments with thrombin were 6.5 /+- 1.5 NIH u/ml and 0.233 /+- 0.012 nmoles MDA/1.4 X 10(9) platelets/min.
journal_name
Thromb Haemostjournal_title
Thrombosis and haemostasisauthors
Rajtar G,Livio M,Merino J,de Gaetano Gsubject
Has Abstractpub_date
1980-10-31 00:00:00pages
49-51issue
2eissn
0340-6245issn
2567-689Xjournal_volume
44pub_type
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
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pub_type: 杂志文章,多中心研究
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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更新日期:1991-05-06 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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更新日期:1992-10-05 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
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pub_type: 杂志文章,meta分析,评审
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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journal_title:Thrombosis and haemostasis
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
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