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Lactate dehydrogenase in human cervical mucus: correlation with ovulation, influence of ovarian steroid hormones, and isozyme pattern.

Abstract：

:The glycolytic enzymes, lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase, and hexokinase in cervical mucus of 96 ovulatory women were serially measured daily during the menstrual cycle. Among all of the enzymes, the cyclic changes in LDH activity were the most significant, being high during the proliferative phase, gradually decreasing to the lowest level around ovulation, and then increasing markedly again. Estrogen inhibited cervical mucus LDH activity; progestrone accelerated it. Examination of the LDH isozyme pattern showed that H-LDH (H-subunit of LDH), being predominant throughout the cycle, apparently carries some responsibility for the cyclic change in LDH activity. In a comparison of LDH activity with the serum luteinizing hormone (LH) peak, the activity level nadir of LDH usually appeared between 1 day before and 1 day after the serum LH peak, pointing to the advantage of daily measurement of cervical mucus LDH activity for predicting the potential day of conception. :Approximately 20% of all causes of inability to conceive by married couples originate in the uterine cervix. This paper examines: 1) cyclic changes in LDH (lactate dehydrogenase) activity in cervical mucus during the menstrual cycle and the effect of hormone treatment on LDH; 2) the effects of ovarian steroid hormones on LDH activity in the cervical mucus; and 3) changes in LDH isozyme patterns in cervical mucus during the menstrual cycle. Multiple cervical mucus samples were obtained from 96 ovulatory women during their menstrual cycle. The women were divided into: 1) untreated group consisting of 46 infertile women; 2) gonadotropin-treated group of 20 women; 3) clomiphene citrate-treated group of 15 patients, and 4) estriol-treated group of 15 patients. In addition to LDH, the following enzymes were serially measured: glycolytic enzymes, glucose-6-phosphate dehydrogenase, and hexokinase. The cyclic changes in LDH activity proved to be the most significant, being high during the proliferative phase, gradually diminishing to the lowest level around ovulation, and then increasing markedly again. Cervical mucus LDH activity was inhibited by estrogen and accelerated by progesterone. Analysis of the LDH isozyme pattern revealed that H-LDH (a subunit of LDH) predominated throughout the cycle and appeared to be responsible for the cyclic changes in LDH activity. The lowest values for cervical mucus LDH usually appeared between 1 day before and 1 day after the serum LH peak. Daily measurement of cervical mucus LDH activity may be of help in predicting potential day of conception.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Takehisa T

doi

10.1016/s0015-0282(16)44533-4

subject

Has Abstract

pub_date

1980-02-01 00:00:00

pages

135-40

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)44533-4

journal_volume

pub_type

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Lactate dehydrogenase in human cervical mucus: correlation with ovulation, influence of ovarian steroid hormones, and isozyme pattern.