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Effects of N6-methyladenosine on the synthesis of phenylethanolamine N-methyltransferase in cultured explants of rat adrenal medulla.

Abstract：

:Explants of adrenal medullae were cultured in defined media for up to 48 h, during which time the tissue remained histologically intact. Addition of N6-methyladenosine to the medium led to a diminution in the activity of phenylethanolamine N-methyltransferase (EC 2.1.1.28) in the tissue. The enzyme activity was inversely proportional to the concentration N6-methyladenosine in the culture medium. The extent of loss of phenylethanolamine N-methyltransferase, as measured by immunochemical titration, corresponded to the degree of loss in enzyme activity under the same conditions. Furthermore, the decreased amount of enzyme protein was due to a decrease in the rate of synthesis of phenylethanolamine N-methyltransferase. Neither adenosine nor several methylated nucleosides, including 7-methylguanosine, N2-methylguanosine, and 5-methylcytosine, had an effect on the enzyme. Two other adrenal medullary enzymes, monoamine oxidase (EC 1.4.3.4) and acid phosphatase (EC 3.1.3.2), were not affected by addition of N6-methyladenosine to the medium. The results are consistent with the view that this effect of N6-methyladenosine on the concentration of phenylethanolamine N-methyltransferase is due to an inhibition of its biosynthesis rather than to an alteration of its rate of degradation.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Burke WJ,Joh TH

doi

10.1111/j.1471-4159.1982.tb04705.x

subject

Has Abstract

pub_date

1982-07-01 00:00:00

pages

92-6

issue

1

eissn

0022-3042

issn

1471-4159

journal_volume

39

pub_type

杂志文章

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