Abstract：
:Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. A significant difference between the fructose 1,6-bisphosphate inhibition constants for both activities was found (Ki(isomerase) = 800 muM and Ki(anomerase) = 140 muM). Also the Km values for both activities were found to be significantly different (Km(isomerase) = 140 muM and Km(anomerase) = 3.6 muM). Attempts to independently alter the anomerase to isomerase activity ratio through protein modification yielded mixed results. While several modifying reagents destroyed the catalytic activities at identical rates, inactivation by iodoacetamide or pyridoxal 5' phosphate sensitized photo-oxidation displayed differential initial effects on the two activities with the anomerase activity being the less affected. These data support the theory that an imidazole residue is catalytically important for isomerization, but less so for anomerization.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Howell EE,Schray KJdoi
10.1007/BF02354933subject
Has Abstractpub_date
1981-07-07 00:00:00pages
101-7issue
2eissn
0300-8177issn
1573-4919journal_volume
37pub_type
杂志文章abstract：:The present study was aimed at addressing the effect of hyperglycemia on antioxidant enzymes. The expression of catalase, superoxide dismutase and glutathione peroxidase, the three primary scavenger enzymes involved in detoxifying reactive oxygen species has been evaluated in the renal cortex of rats after 6 weeks of ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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更新日期：1989-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1993-08-11 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1990-07-17 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/BF00285223
更新日期：1984-09-01 00:00:00
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更新日期：2013-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230808
更新日期：1991-01-16 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1007048313556
更新日期：2000-06-01 00:00:00
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更新日期：2003-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1993-02-17 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1993-07-07 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1993-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1976-09-30 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1998-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1225-y
更新日期：2012-05-01 00:00:00
