Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.

abstract：:The three-dimensional crystal structure of variant-3 toxin from the scorpion Centruroides sculpturatus Ewing has been determined at 3 A resolution. Phases were obtained by use of K2PtCl4 and K2IrCl6 derivatives. The most prominent secondary structural features are two and a half turns of alpha-helix and a three-strand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fontecilla-Camps JC,Almassy RJ,Suddath FL,Watt DD,Bugg CE

更新日期：1980-11-01 00:00:00

abstract：:Zea mays L. ssp. mays, or corn, one of the most important crops and a model for plant genetics, has a genome approximately 80% the size of the human genome. To gain global insight into the organization of its genome, we have sequenced the ends of large insert clones, yielding a cumulative length of one-eighth of the g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Messing J,Bharti AK,Karlowski WM,Gundlach H,Kim HR,Yu Y,Wei F,Fuks G,Soderlund CA,Mayer KF,Wing RA

更新日期：2004-10-05 00:00:00

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:Insulin/Insulin-like growth factor signaling regulates homeostasis and growth in mammals, and is implicated in diseases from diabetes to cancer. In Drosophila melanogaster, as in other invertebrates, multiple Insulin-Like Peptides (DILPs) are encoded by a family of related genes. To assess DILPs' physiological roles, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang H,Liu J,Li CR,Momen B,Kohanski RA,Pick L

更新日期：2009-11-17 00:00:00

abstract：:The reported isolation of nanobacteria from human kidney stones raises the intriguing possibility that these microorganisms are etiological agents of pathological extraskeletal calcification [Kajander, E. O. & Ciftçioglu, N. (1998) Proc. Natl. Acad. Sci. USA 95, 8274-8279]. Nanobacteria were previously isolated from F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cisar JO,Xu DQ,Thompson J,Swaim W,Hu L,Kopecko DJ

更新日期：2000-10-10 00:00:00

abstract：:To test the hypothesis that the nonrandom organization of the contents of interphase nuclei represents a compartmentalization of function, we examined the relative, spatial relationship of small nuclear ribonucleoproteins (snRNPs) and of DNase I hypersensitive chromatin (DHC) in rat pheochromocytoma cells. In controls...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park PC,De Boni U

更新日期：1996-10-15 00:00:00

abstract：:The precise determination of de novo genetic variants has enormous implications across different fields of biology and medicine, particularly personalized medicine. Currently, de novo variations are identified by mapping sample reads from a parent-offspring trio to a reference genome, allowing for a certain degree of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gómez-Romero L,Palacios-Flores K,Reyes J,García D,Boege M,Dávila G,Flores M,Schatz MC,Palacios R

更新日期：2018-05-22 00:00:00

abstract：:Accumulating evidence indicates that epigenetic alterations contribute to exacerbated activation or deregulation of the mechanisms that maintain tolerance to self-antigens in patients with lupus, a systemic autoimmune disease that can be triggered by medications taken to treat a variety of conditions. Here, we tested ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazari L,Ouarzane M,Zouali M

更新日期：2007-04-10 00:00:00

abstract：:We have investigated the effect of the v-Myc oncoprotein on gene expression in myelomonocytic cells. We find that v-Myc dramatically down-regulates the expression of myelomonocytic-specific genes, such as the chicken mim-1 and lysozyme genes, both of which are known targets for C/EBP transcription factors. We present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mink S,Mutschler B,Weiskirchen R,Bister K,Klempnauer KH

更新日期：1996-06-25 00:00:00

abstract：:A cofactor needed for the reduction of CDP-4-keto-6-deoxy-D-glucose to CDP-4-keto-3,6-dideoxy D-glucose has been detected and purified. Its spectra in neutral, acid, and basic solutions, its behavior on thin-layer chromatography, and other properties led to its identification as pyridoxamine-5'-phosphate. Moreover, au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonzalez-Porque P,Strominger JL

更新日期：1972-06-01 00:00:00

abstract：:Unlike normal cells, malignant rat and two simian virus 40-transformed human cell lines can neither grow nor survive in B12-and folate-supplemented media in which methionine is replaced by homocysteine. Yet three lines of evidence indicate that the malignant and transformed cells synthesize large amounts of methionine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman RM,Erbe RW

更新日期：1976-05-01 00:00:00

abstract：:We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences between an initially f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gelfand CA,Plum GE,Mielewczyk S,Remeta DP,Breslauer KJ

更新日期：1999-05-25 00:00:00

abstract：:The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, on Na+ influx were investigated in cultured human foreskin fibroblasts (HSWP cells). We report here that in serum-deprived HSWP cells the addition of PMA alone has no significant effect on Na+ influx. However, the addition of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vicentini LM,Villereal ML

更新日期：1985-12-01 00:00:00

abstract：:A regeneration chamber was created in vivo by suturing a synthetic tube sealed at its distal end onto the proximal stump of a severed rat sciatic nerve. Nerves regenerated into tubes coated with laminin at a rate of 0.33 mm/day after a lag of about 2 days. At 25 days, regenerating nerves had extended 23% farther into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toyota B,Carbonetto S,David S

更新日期：1990-02-01 00:00:00

abstract：:In this study we compared the cellular concentrations and stability of the mRNA transcribed from the aprE (subtilisin) gene (a gene preferentially expressed in stationary growth phase) with those of a vegetative mRNA, succinate dehydrogenase (SDH) mRNA. The subtilisin transcript was shown to be at least 3 times more s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Resnekov O,Rutberg L,von Gabain A

更新日期：1990-11-01 00:00:00

abstract：:Ischemia and reperfusion both contribute to tissue damage after myocardial infarction. Although many drugs have been shown to reduce infarct size when administered before ischemia, few have been shown to be effective when administered at reperfusion. Moreover, although it is generally accepted that a burst of reactive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Granville DJ,Tashakkor B,Takeuchi C,Gustafsson AB,Huang C,Sayen MR,Wentworth P Jr,Yeager M,Gottlieb RA

更新日期：2004-02-03 00:00:00

abstract：:We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alwine JC,Kemp DJ,Stark GR

更新日期：1977-12-01 00:00:00

abstract：:Recoverin, a recently discovered member of the EF-hand superfamily of Ca(2+)-binding proteins, serves as a Ca2+ sensor in vision. The amino terminus of the protein from retinal rod cells contains a covalently attached myristoyl or related N-acyl group. We report here studies of unmyristoylated and myristoylated recomb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zozulya S,Stryer L

更新日期：1992-12-01 00:00:00

abstract：:Many fatal neurodegenerative diseases such as Alzheimer's, Parkinson, the prion-related diseases, and non-neurodegenerative disorders such as type II diabetes are characterized by abnormal amyloid fiber aggregates, suggesting a common mechanism of pathogenesis. We have discovered that a class of systematically designe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hauser CA,Deng R,Mishra A,Loo Y,Khoe U,Zhuang F,Cheong DW,Accardo A,Sullivan MB,Riekel C,Ying JY,Hauser UA

更新日期：2011-01-25 00:00:00

abstract：:A site that is preferentially cleaved by the single-strand-specific endonuclease from Aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(I) collagen gene. It is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finer MH,Fodor EJ,Boedtker H,Doty P

更新日期：1984-03-01 00:00:00

abstract：:The TFIID transcription initiation complex is composed of TBP and multiple TAFs. Studies in unicellular systems indicate that TAF250 is required for progression through G(1)/S of the cell cycle and repression of apoptosis. Here we extend these in vivo studies by determining the developmental requirements for TAF250 in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wassarman DA,Aoyagi N,Pile LA,Schlag EM

更新日期：2000-02-01 00:00:00

abstract：:Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter CP,Warner CL

更新日期：1974-03-01 00:00:00

abstract：:Familial multiple system tauopathy with presenile dementia (MSTD) is a neurodegenerative disease with an abundant filamentous tau protein pathology. It belongs to the group of familial frontotemporal dementias with Parkinsonism linked to chromosome 17 (FTDP-17), a major class of inherited dementing disorders whose gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spillantini MG,Murrell JR,Goedert M,Farlow MR,Klug A,Ghetti B

更新日期：1998-06-23 00:00:00

abstract：:FKBP12-rapamycin associated protein (FRAP, also known as mTOR or RAFT) is the founding member of the phosphatidylinositol kinase-related kinase family and functions as a sensor of physiological signals that regulate cell growth. Signals integrated by FRAP include nutrients, cAMP levels, and osmotic stress, and cellula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Desai BN,Myers BR,Schreiber SL

更新日期：2002-04-02 00:00:00

abstract：:Adaptation of photosynthesis in marine environment has been examined in two strains of the green, picoeukaryote Ostreococcus: OTH95, a surface/high-light strain, and RCC809, a deep-sea/low-light strain. Differences between the two strains include changes in the light-harvesting capacity, which is lower in OTH95, and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cardol P,Bailleul B,Rappaport F,Derelle E,Béal D,Breyton C,Bailey S,Wollman FA,Grossman A,Moreau H,Finazzi G

更新日期：2008-06-03 00:00:00

abstract：:Entamoeba histolytica is a single cell eukaryote that is the etiologic agent of amoebic colitis. Core promoter elements of E. histolytica protein encoding genes include a TATA-like sequence (GTATTTAAAG/C) at -30, a novel element designated GAAC (GAACT) that has a variable location between TATA and the site of transcri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh U,Rogers JB,Mann BJ,Petri WA Jr

更新日期：1997-08-05 00:00:00

abstract：:Factor VII is a precursor to a serine protease that is present in mammalian plasma. In its activated form, it participates in blood coagulation by activating factor X and/or factor IX in the presence of tissue factor and calcium. Clones coding for factor VII were obtained from two cDNA libraries prepared from poly(A) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hagen FS,Gray CL,O'Hara P,Grant FJ,Saari GC,Woodbury RG,Hart CE,Insley M,Kisiel W,Kurachi K

更新日期：1986-04-01 00:00:00

abstract：:Recent evidence indicates there is a role for small membrane vesicles, including exosomes, as vehicles for intercellular communication. Exosomes secreted by most cell types can mediate transfer of proteins, mRNAs, and microRNAs, but their role in the transmission of infectious agents is less established. Recent studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramakrishnaiah V,Thumann C,Fofana I,Habersetzer F,Pan Q,de Ruiter PE,Willemsen R,Demmers JA,Stalin Raj V,Jenster G,Kwekkeboom J,Tilanus HW,Haagmans BL,Baumert TF,van der Laan LJ

更新日期：2013-08-06 00:00:00

abstract：:The protein products of T4 bacteriophage genes 41, 43, 45, 44, and 62 have been purified to near homogeneity using an assay which measures their stimulation of DNA synthesis in a crude lysate of Escherichia coli cells in fected by an appropriate mutant phage. When all of these proteins and T4 gene 32 protein are incub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris CF,Sinha NK,Alberts BM

更新日期：1975-12-01 00:00:00

abstract：:The high susceptibility of human immunoglobulin D to proteolytic degradation affects its biological function, metabolism, and immunoassay. High-pressure liquid chromatography was used to investigate the mechanism and rate of limited proteolytic cleavage of IgD and also to identify, isolate, and quantify the reaction p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishioka N,Takahashi N,Putnam FW

更新日期：1987-01-01 00:00:00