Abstract:
:We have cloned and identified a DNA sequence complementary to the mRNA of creatine kinase (CK) isozyme M, although the mRNA is a minor species of the total mRNA in developing myoblasts. Poly(A)+RNA from breast and thigh muscle of 5-week-old chicks was enriched for CK mRNA by a novel procedure of sucrose gradient centrifugation in the presence of methylmercuric hydroxide. DNA complementary to this mRNA was inserted into pBR322, and colonies containing the recombinant plasmids were screened for the ability of the plasmid DNA to hybridize with and rescue CK mRNA from total muscle mRNA. Three plasmids, pCS195, pCS192, and pM35-4, could specifically rescue CK-M mRNA. CK-M mRNA was detected by in vitro translation and specific immunoprecipitation. The identity of the in vitro translation product was further confirmed by its migration in two-dimensional gels at the isoelectric point and molecular weight of CK-M. The heterogeneity of CK-M observed in vivo also was found upon translation of the CK-M mRNA which hybridizes to the plasmid.
journal_name
Proc Natl Acad Sci U S Aauthors
Schweinfest CW,Kwiatkowski RW,Dottin RPdoi
10.1073/pnas.79.16.4997subject
Has Abstractpub_date
1982-08-01 00:00:00pages
4997-5000issue
16eissn
0027-8424issn
1091-6490journal_volume
79pub_type
杂志文章abstract::More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered. Upon light-induced changes in ligand-receptor interaction, movement of specific transmembrane helices within the receptor opens a crevice at the cytoplasm...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2018-01-23 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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