Migration of human leukocytes from soft agarose droplet: a simplified method for studying chemotaxis and spontaneous migration.

Abstract:

:Studies of in vitro chemotaxis and spontaneous migration of human leukocytes using the accepted method with the Boyden-chamber-filter are troublesome, because of the need for specially constructed vessels as well as the difficulties caused by the use of membrane filters. We describe a new and simplified method for measuring human leucocyte chemotaxis, which is a modification of the recently described underagarose migration method and which is based upon spontaneous migration of cells from a soft agarose droplet and in response to a chemotactic gradient. We examined suspensions of leukocytes, purified granulocytes, and mononuclear cells from 10 healthy normal adults and from 10 samples of cord blood using E Coli O111B4 endotoxin-activated human serum as attractant. Our results showed that the mean chemotactic indices (C.I.-chemotaxis/migration) for purified granulocytes and for mononuclear cells from normal individuals were 3.0 +/- 1.2 and 2.7 +/- 1.5, respectively. Chemotaxis was significantly reduced when unwashed leukocytes were studied, indicating a detrimental effect of autologous plasma on leukocytic response to a chemotactic stimulus in this system. Cord blood cells showed normal spontaneous migration, but significantly decreased chemotaxis. This preliminary report shows that the technique is simple, rapid, and reproducible, and can detect abnormalities of chemotaxis in both granulocytes and mononuclear cells.

journal_name

Am J Hematol

authors

Barak Y,Karov Y,Levin S

doi

10.1002/ajh.2830140308

subject

Has Abstract

pub_date

1983-05-01 00:00:00

pages

271-8

issue

3

eissn

0361-8609

issn

1096-8652

journal_volume

14

pub_type

杂志文章
  • Genomic Characteristics and Prognostic Significance of Co-mutated ASXL1/SRSF2 Acute Myeloid Leukemia.

    abstract::ASXL1 and SRSF2 mutations in AML are frequently found in patients with preexisting myeloid malignancies and are individually associated with poor outcomes. In this multi-institutional retrospective analysis, we assessed the genetic features and clinical outcomes of 43 patients with ASXL1mut SRSF2mut AML and compared o...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.26110

    authors: Richardson DR,Swoboda DM,Moore DT,Johnson SM,Chan O,Galeotti J,Esparza S,Hussaini MO,Van Deventer H,Foster MC,Coombs CC,Montgomery ND,Sallman DA,Zeidner JF

    更新日期:2021-01-27 00:00:00

  • The addition of dexamethasone to bortezomib for patients with relapsed multiple myeloma improves outcome but ongoing maintenance therapy has minimal benefit.

    abstract::Despite the common practice of combining dexamethasone (Dex) with bortezomib (Bz) in patients with multiple myeloma (MM), until now there has been few prospective trials undertaken. We undertook a trial that recapitulated the original APEX study except that dexamethasone was incorporated from cycle 1. We also incorpor...

    journal_title:American journal of hematology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1002/ajh.23967

    authors: Harrison SJ,Quach H,Link E,Feng H,Dean J,Copeman M,Van De Velde H,Schwarer A,Baker B,Spencer A,Catalano J,Campbell P,Augustson B,Romeril K,Prince HM

    更新日期:2015-05-01 00:00:00

  • Utility and prognostic value of 18 F-FDG positron emission tomography-computed tomography scans in patients with newly diagnosed multiple myeloma.

    abstract::Positron emission tomography-computed tomography (PET-CT) can identify bony lesions, assess disease burden, and detect extramedullary disease (EMD) in patients with multiple myeloma. We retrospectively reviewed records of patients who underwent PET-CT within 60 days of a new diagnosis (before therapy commenced) to ide...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.25279

    authors: Aljama MA,Sidiqi MH,Buadi FK,Lacy MQ,Gertz MA,Dispenzieri A,Dingli D,Muchtar E,Fonder AL,Hayman SR,Hobbs MA,Gonsalves WI,Warsame RM,Kourelis T,Hwa YL,Kapoor P,Kyle RA,Leung N,Go RS,Rajkumar SV,Kumar SK

    更新日期:2018-12-01 00:00:00

  • Monocytic leukemia cutis diagnosed simultaneously with refractory anemia with monocytosis: a case report.

    abstract::A case of leukemia cutis (LC) of monocytic lineage in a patient with myelodysplastic syndrome (MDS) is presented. Cutaneous infiltrates were recognized concurrent with diagnosis of refractory anemia (RA) with monocytosis. Skin infiltrates subsequently spontaneously regressed although MDS progressed with increasing mon...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/1096-8652(200102)66:2<120::AID-AJH1027>3.0

    authors: Yavorkovsky LL,Zain J,Wu CD,Trivelli L,Cook P

    更新日期:2001-02-01 00:00:00

  • High serum lactate dehydrogenase level predicts short survival after vincristine-doxorubicin-dexamethasone (VAD) salvage for refractory multiple myeloma.

    abstract::We evaluated possible prognostic factors just before salvage therapy with vincristine, doxorubicin, and dexamethasone (VAD) for 36 patients with refractory multiple myeloma. The median duration from diagnosis to the first VAD salvage was 14 months (range 2-76 months). Among parameters that have been shown to be associ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/1096-8652(200010)65:2<132::aid-ajh7>3.0.co

    authors: Suguro M,Kanda Y,Yamamoto R,Chizuka A,Hamaki T,Matsuyama T,Takezako N,Miwa A,Togawa A

    更新日期:2000-10-01 00:00:00

  • Enhancement of sickle erythrocyte adherence to endothelium by autologous platelets.

    abstract::The increased adhesiveness of sickle erythrocytes (SS RBC) to endothelial cells has been confirmed in a static system utilizing fresh umbilical vein endothelium. Adherence of SS RBC to the endothelium was as great in the presence of calcium-containing buffer as when incubated in plasma. SS RBC suspended in autologous ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830340110

    authors: Antonucci R,Walker R,Herion J,Orringer E

    更新日期:1990-05-01 00:00:00

  • A single-center experience in 20 patients with infantile malignant osteopetrosis.

    abstract::Infantile malignant osteopetrosis (IMO) includes various genetic disorders that affect osteoclast development and/or function. Genotype-phenotype correlation studies in IMO have been hampered by the rarity and heterogeneity of the disease and by the severity of the clinical course, which often leads to death early in ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.21447

    authors: Mazzolari E,Forino C,Razza A,Porta F,Villa A,Notarangelo LD

    更新日期:2009-08-01 00:00:00

  • Isolation and characterization of granulocyte lysosomal proteins and study of their effects on the clotting system.

    abstract::Lysosomes (granules) of rabbit PMN leukocytes were extracted with either HCl or H2SO4, and the extracts were chromatographed over Sephadex to separate protein constituents. Some of the low molecular weight cationic proteins homogeneous on SDS PAGE (8% and 12.5% gels) were characterized by electrophoretic mobility in a...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830070309

    authors: Herion JC,Bucher JR,Penniall R,Walker RI,Baker M,Roberts HR

    更新日期:1979-01-01 00:00:00

  • Mutant calreticulin-expressing cells induce monocyte hyperreactivity through a paracrine mechanism.

    abstract::Mutations in the calreticulin gene (CALR) were recently identified in approximately 70-80% of patients with JAK2-V617F-negative essential thrombocytosis and primary myelofibrosis. All frameshift mutations generate a recurring novel C-terminus. Here we provide evidence that mutant calreticulin does not accumulate effic...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.24245

    authors: Garbati MR,Welgan CA,Landefeld SH,Newell LF,Agarwal A,Dunlap JB,Chourasia TK,Lee H,Elferich J,Traer E,Rattray R,Cascio MJ,Press RD,Bagby GC,Tyner JW,Druker BJ,Dao KH

    更新日期:2016-02-01 00:00:00

  • Addition of cladribine to the standard induction treatment improves outcomes in a subset of elderly acute myeloid leukemia patients. Results of a randomized Polish Adult Leukemia Group (PALG) phase II trial.

    abstract::Intensive induction chemotherapy using anthracycline and cytarabine backbone is considered the most effective upfront therapy in physically fit older patients with acute myeloid leukemia (AML). However, outcomes of the standard induction in elderly AML are inferior to those observed in younger patients, and they are s...

    journal_title:American journal of hematology

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/ajh.24654

    authors: Pluta A,Robak T,Wrzesien-Kus A,Katarzyna Budziszewska B,Sulek K,Wawrzyniak E,Czemerska M,Zwolinska M,Golos A,Holowiecka-Goral A,Kyrcz-Krzemien S,Piszcz J,Kloczko J,Mordak-Domagala M,Lange A,Razny M,Madry K,Wiktor-Jedrze

    更新日期:2017-04-01 00:00:00

  • Does iron overload really matter in stem cell transplantation?

    abstract::A growing body of evidence suggests that iron overload is associated with inferior outcomes after myeloablative allogeneic hematopoietic stem cell transplantation (HSCT). However, all of those studies used surrogate markers of iron overload, especially serum ferritin, and most had a retrospective design. We conducted ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23188

    authors: Armand P,Sainvil MM,Kim HT,Rhodes J,Cutler C,Ho VT,Koreth J,Alyea EP,Neufeld EJ,Kwong RY,Soiffer RJ,Antin JH

    更新日期:2012-06-01 00:00:00

  • Immunoglobulin light chain amyloidosis: 2020 update on diagnosis, prognosis, and treatment.

    abstract:DISEASE OVERVIEW:Immunoglobulin light chain amyloidosis is a clonal, nonproliferative plasma cell disorder in which fragments of immunoglobulin light or heavy chain are deposited in tissues. Clinical features depend on organs involved but can include heart failure with preserved ejection fraction, nephrotic syndrome, h...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.25819

    authors: Gertz MA

    更新日期:2020-07-01 00:00:00

  • High prevalence of thrombocytopenia in SLE patients with a high level of anticardiolipin antibodies combined with lupus anticoagulant.

    abstract::The relationship between thrombocytopenia and the level of anticardiolipin antibodies (aCL) and/or the existence of lupus anticoagulant (LA) ware studied in 146 patients with systemic lupus erythematosus (SLE). These patients were divided into six groups: A, those LA positive with a high level of aCL (>10 U/ml) (10 ca...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-8652(199805)58:1<55::aid-ajh10>

    authors: Nojima J,Suehisa E,Kuratsune H,Machii T,Toku M,Tada H,Yamaguti K,Koike T,Kanakura Y,Kitani T,Amino N

    更新日期:1998-05-01 00:00:00

  • Platelet interaction with bacteria. VI. contrasting the role of fibrinogen and fibronectin.

    abstract::The roles of fibrinogen and fibronectin were contasted in the responses of human platelets to Staphylococcus aureus and collagen. Congenital afibrinogenemic (CA) platelets and washed normal platelets had delayed aggregation due to a prolonged latent phase in response to contact with the bacteria when fibrinogen was ab...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830090106

    authors: Clawson CC,White JG,Herzberg MC

    更新日期:1980-01-01 00:00:00

  • Elevated serum free light chains are associated with inferior event free and overall survival in Hodgkin lymphoma.

    abstract::The serum free light chain (FLC) assay quantitates free immunoglobulin kappa and lambda light chains, which has prognostic value in plasma cell dyscrasias. However, there is limited data on serum FLC in lymphoid malignancies. We analyzed the association of pretreatment FLC with event-free survival (EFS) and overall su...

    journal_title:American journal of hematology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ajh.22168

    authors: Thompson CA,Maurer MJ,Cerhan JR,Katzmann JA,Ansell SM,Habermann TM,Macon WR,Weiner GJ,Link BK,Witzig TE

    更新日期:2011-12-01 00:00:00

  • Postoperative thrombotic thrombocytopenic purpura following cardiovascular surgeries.

    abstract::Among 47 patients with thrombotic thrombocytopenic purpura (TTP), 8 patients were diagnosed to have postoperative-TTP. Two patients underwent vascular surgery, 5 patients coronary artery bypass grafts, and 1 patient resection of myocardial sarcoma. Prior to surgery, all patients except one had normal hemograms and pla...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-8652(199609)53:1<11::AID-AJH3>3

    authors: Chang JC,Shipstone A,Llenado-Lee MA

    更新日期:1996-09-01 00:00:00

  • Natural history of acquired immunodeficiency syndrome in hemophilic patients.

    abstract::During the 5-year period from 1981 to 1985, we have observed 8 cases of acquired immunodeficiency syndrome (AIDS) among our 85 patients with hemophilia A. Thus, the prevalence of AIDS with hemophilia A is 9.4% in our patient population. By utilizing stored serum or plasma samples dating back to 1978, antibody against ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830240208

    authors: Kim HC,Nahum K,Raska K Jr,Gocke DJ,Kosmin M,Karp GI,Saidi P

    更新日期:1987-02-01 00:00:00

  • Serum from outdated human platelet concentrates: an alternative supplement for tissue (fibroblast) culture media.

    abstract::Fetal calf serum (FCS) is used as a component of the media for cells grown in culture. However, the high cost of FCS has stimulated a search for a possible alternative. Approximately 10% of human platelet concentrates prepared for clinical transfusions are outdated. Sera prepared from platelet concentrates were compar...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830170104

    authors: Schwartz KA,Lu G,Trosko JE,Chang CC

    更新日期:1984-07-01 00:00:00

  • Disseminated intravascular coagulation: clinical and laboratory aspects.

    abstract::Disseminated intravascular coagulation (DIC) is a complex acquired coagulopathy resulting from excessive thrombin formation. Abnormal tissue factor (TF) expression is a major mechanism initiating DIC in many disorders, including obstetrical complications, sepsis, cancer, and trauma. Numerous laboratory tests are avail...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1096-8652(199809)59:1<65::aid-ajh13>

    authors: Carey MJ,Rodgers GM

    更新日期:1998-09-01 00:00:00

  • High NPM1 mutant allele burden at diagnosis correlates with minimal residual disease at first remission in de novo acute myeloid leukemia.

    abstract::Acute myeloid leukemia (AML) with mutated NPM1 is a newly recognized separate entity in the revised 2016 WHO classification, and is associated with a favorable prognosis. While previous studies have evaluated NPM1 in a binary fashion, we recently demonstrated a significant independent negative prognostic effect of hig...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.25544

    authors: Patel SS,Pinkus GS,Ritterhouse LL,Segal JP,Dal Cin P,Restrepo T,Harris MH,Stone RM,Hasserjian RP,Weinberg OK

    更新日期:2019-08-01 00:00:00

  • Rituximab-cyclophosphamide-dexamethasone is highly effective in patients with monoclonal Ig deposit-related glomerulopathy and indolent non-Hodgkin lymphomas.

    abstract::Indolent non-hodgkin lymphomas (iNHL) are a rare cause of monoclonal immunoglobulin deposits-related glomerulopathy (mIgGN). In patients with iNHL-related mIgGN, whether treatment should include either single or a combination of drug(s) to target the malignant clone and renal inflammation remains elusive. In this retr...

    journal_title:American journal of hematology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1002/ajh.23798

    authors: Perry M,Delarche A,Ribes D,Vergez F,Guilbeau-Frugier C,Laurent C,Huart A,Tavitian S,El Hachem H,Oberic L,Chauveau D,Michallet AS,Ysebaert L,Faguer S

    更新日期:2014-10-01 00:00:00

  • Splenectomy for thrombocytopenia in chronic lymphocytic leukemia.

    abstract::The role of peripheral platelet destruction as a reversible etiology of thrombocytopenia in chronic lymphocytic leukemia (CLL) was evaluated in nine patients with CLL and refractory thrombocytopenia who underwent splenectomy. The patients' ages ranged from 54 to 74 years. Progressive thrombocytopenia refractory to ant...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830150306

    authors: Merl SA,Theodorakis ME,Goldberg J,Gottlieb AJ

    更新日期:1983-11-01 00:00:00

  • Hereditary hemochromatosis and red cell aplasia.

    abstract::A 63-year-old white man with myasthenia gravis and red cell aplasia was found to have hepatic iron overload. The differential diagnosis included hereditary hemochromatosis and secondary iron overload related to red cell aplasia. Family studies of siblings, including HLA typing, initially suggested a diagnosis of secon...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830450314

    authors: Adams PC

    更新日期:1994-03-01 00:00:00

  • An introduction to chimeric antigen receptor (CAR) T-cell immunotherapy for human cancer.

    abstract::Chimeric antigen receptor (CAR) T-cell therapy represents a major advancement in personalized cancer treatment. In this strategy, a patient's own T cells are genetically engineered to express a synthetic receptor that binds a tumor antigen. CAR T cells are then expanded for clinical use and infused back into the patie...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.25418

    authors: Feins S,Kong W,Williams EF,Milone MC,Fraietta JA

    更新日期:2019-05-01 00:00:00

  • Baseline levels of plasma endothelin-1 (ET-1) and changes during transfusion in thalassemic patients.

    abstract::We studied levels of plasma endothelin-1 (ET-1) in 25 beta-thalassemia/Hb E patients before and after blood transfusion. Baseline ET-1 levels in these patients were significantly higher than in normal controls (10.17 +/- 2.1 pg/mL vs. 8.9 +/- 2.0 pg/mL, P < 0.05). After blood transfusion, levels of plasma ET-1 tend to...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.10129

    authors: Viprakasit V,Kankirawatana S,Akarasereenont P,Durongpisitkul K,Chotewuttakorn S,Tanphaichitr VS

    更新日期:2002-07-01 00:00:00

  • Functional analysis of the marginating pool of human polymorphonuclear leukocytes.

    abstract::The intravascular pool of human polymorphonuclear leukocytes (PMN) is composed of one compartment which is circulating and another that is marginated to the vascular endothelium. Administration of B-adrenergic agonists leads to a rapid demargination with an increase in the circulating PMN pool. The marginating PMN has...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830240107

    authors: Berkow RL,Dodson RW

    更新日期:1987-01-01 00:00:00

  • Detection of platelet associated IgG in immune thrombocytopenia: a new assay employing protein A and peroxidase anti-peroxidase (PROA-PAP).

    abstract::Immune thrombocytopenia is frequently encountered in medical practice and is generally accepted as being caused by an IgG antibody. The capability of detecting platelet-bound IgG as a diagnostic and therapeutic modality is critical for appropriate care and management of patients with idiopathic thrombocytopenic purpur...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830090402

    authors: Tate DY,Carlton GT,Nesbit ME,White JG,Krivit W,Sorenson RL

    更新日期:1980-01-01 00:00:00

  • Haplotypes and levels of fetal hemoglobin and G gamma to A gamma ratios in Mediterranean patients with thalassemia minor and major.

    abstract::This study concerned the gamma chain composition of Hb F and the haplotypes of 44 patients with beta-thalassemia major or intermedia and many of their relatives. Seventeen patients came from Northern (Turkish) Cyprus, 12 from the Istanbul area, and 15 from Macedonia and Bulgaria. Analysis of the A gamma T-G gamma-A ga...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830200103

    authors: Aksoy M,Kutlar A,Efremov GD,Nikolov N,Petkov G,Reese AL,Harano T,Chen SS,Huisman TH

    更新日期:1985-09-01 00:00:00

  • Systemic amyloidosis associated with chronic lymphocytic leukemia/small lymphocytic lymphoma.

    abstract::To clarify the presentation and course of patients with chronic lymphocytic leukemia (CLL) and amyloidosis. Mayo databases were interrogated for patients who carried a diagnosis of amyloidosis and CLL evaluated at Mayo Clinic, Rochester from January 1974 to October 2012. Charts were abstracted and data analyzed. Of th...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23413

    authors: Kourelis TV,Gertz M,Zent C,Lacy M,Kyle R,Kapoor P,Zeldenrust S,Buadi F,Witzig T,Hayman S,Lust J,Russell S,Lin Y,Rajkumar VS,Kumar S,Leung N,Dingli D,Dispenzieri A

    更新日期:2013-05-01 00:00:00

  • Phenotypic variability of Filipino beta(o)-thalassemia/HbE patients in Indonesia.

    abstract::Three Indonesian patients with identical genotypes, each compound heterozygotes for Filipino beta(o)-thalassemia/HbE, expressed different clinical severities. One patient has mild disease and is transfusion independent, while the other two are severely affected and transfusion dependent. The size of the Filipino beta(...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-8652(199909)62:1<7::aid-ajh2>3.

    authors: Setianingsih I,Williamson R,Daud D,Harahap A,Marzuki S,Forrest S

    更新日期:1999-09-01 00:00:00