Abstract：
:In dissociated hypothalamic cell cultures of 16-day mouse embryos, growing in chemically defined medium, the catecholaminergic neurons were identified by autoradiography after labelling with [3H]dopamine and by immunocytochemistry with an anti-tyrosine hydroxylase antibody. Using selective inhibitors of amine transport and radioenzymatic determination of amine levels in these cultures, we show that these neurons were mostly dopaminergic. The number of dopaminergic neurons identified by the two techniques increased between days 5 and 8 and decreased after 15 days in vitro. The same number of neurons were identified by autoradiography and by immunocytochemistry and consisted of fusiform and multipolar neurons. The proportion of both types remained steady until 15 days in vitro. Under these conditions, the addition of triiodothyronine (10(-9) M) at the initiation of the culture increased the size but not the number of dopaminergic neurons after 8 days in vitro. Furthermore, triiodothyronine significantly increased the dopaminergic neurite length and arborization. This morphological effect of triiodothyronine was associated with an increase of 35% in [3H]dopamine uptake. Our study shows that hypothalamic dopaminergic neurons are responsive to triiodothyronine which acts as a maintenance or trophic factor having an effect on neurite extension and arborization.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Puymirat J,Barret A,Picart R,Vigny A,Loudes C,Faivre-Bauman A,Tixier-Vidal Adoi
10.1016/0306-4522(83)90217-8subject
Has Abstractpub_date
1983-11-01 00:00:00pages
801-10issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(83)90217-8journal_volume
10pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:Components of the brain's dopaminergic system, such as dopamine receptors, undergo final maturation in adolescence. Exposure to social stress during human adolescence contributes to substance abuse behaviors. We utilized a rat model of adolescent social stress to investigate the neural mechanisms underlying this corre...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.09.008
更新日期：2011-12-01 00:00:00
abstract：:The recognition molecule myelin-associated glycoprotein is expressed by oligodendrocytes, the myelinating cells of the central nervous system. The myelin-associated glycoprotein gene gives rise to two alternatively spliced transcript variants ("early" and "late" message) which are developmentally regulated. In this st...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00586-6
更新日期：1997-05-01 00:00:00
abstract：:Growth-associated protein-43, an established marker of neuronal plasticity during development and in injury, was used to characterize innervation in the normal human pancreas and changes in chronic alcohol-induced pancreatitis by using light microscopic immunocytochemistry and computer-assisted image analysis. Immunos...
journal_title：Neuroscience
pub_type： 临床试验,杂志文章
doi：10.1016/0306-4522(94)90020-5
更新日期：1994-11-01 00:00:00
abstract：:Horizontal and amacrine cells in the isolated carp retina were impaled with micropipette electrode, identified by their characteristic light responses, and injected iontophoretically with markers for morphological study. Both Lucifer Yellow CH and biocytin were injected simultaneously. Lucifer Yellow was seen by its o...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90112-0
更新日期：1994-03-01 00:00:00
abstract：:The temporal pattern of distribution of somatostatin receptor was investigated using the somatostatin analogue [125I]Tyr0-DTrp8-somatostatin14 as a ligand and compared with that of somatostatin immunoreactivity during early developmental stages in the spinal cord and the sensory derivatives in rat fetuses. Qualitative...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90335-2
更新日期：1994-09-01 00:00:00
abstract：:Updating the position of an earth-fixed target during whole-body rotation seems to rely on cognitive processes such as the utilization of external feedback. According to perceptual learning models, improvement in performance can also occur without external feedback. The aim of this study was to assess spatial updating...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.05.024
更新日期：2015-08-06 00:00:00
abstract：:Nitric oxide (NO) has been long assumed to play a key role in mammalian olfaction. This was based largely on circumstantial evidence, i.e. prominent staining for nitric oxide synthase (NOS) and cyclic guanosine 3',5'-cyclic monophosphate (cGMP) or soluble guanylyl cyclase, an effector enzyme activated by NO, in local ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2008.03.003
更新日期：2008-05-15 00:00:00
abstract：:A systematic line-sampling method is described for counting and mapping myelinated axons in transverse sections of the spinal cord. Its advantages over random sampling of small areas are considered. The technique was applied to quantifying experimental weight-drop contusion injuries of cat spinal cord, from several mo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(83)90150-1
更新日期：1983-10-01 00:00:00
abstract：:In mice, microRNAs (miRNAs) are required for embryonic viability, and previous reports implicate miRNA participation in brain cortical neurogenesis. Here, we provide a more comprehensive analysis of miRNA involvement in cortical brain development. To accomplish this we used mice in which Dicer, the RNase III enzyme ne...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.08.009
更新日期：2012-10-25 00:00:00
abstract：:Midbrain dopamine neurons in the ventral tegmental area, substantia nigra and retrorubral field play key roles in reward processing, learning and memory, and movement. Within these midbrain regions and admixed with the dopamine neurons, are also substantial populations of GABAergic neurons that regulate dopamine neuro...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2008.01.046
更新日期：2008-04-09 00:00:00
abstract：:Brain ischemia induces a marked response of resident microglia and hematopoietic cells including monocytes/macrophages. The present study was designed to assess the distribution of microglia/macrophages in cerebral ischemia using bone marrow chimera mice known to express enhanced green fluorescent protein (EGFP). At 2...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00954-5
更新日期：2003-01-01 00:00:00
abstract：:A diet consisting of high levels of saturated fat has been linked to a dramatic rise in obesity, type II diabetes, and metabolic syndrome. The effect of these co-morbidities on stroke outcome has not been examined in detail in human or animal studies. In this study we hypothesized that maintaining animals on a high fa...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.03.028
更新日期：2011-05-19 00:00:00
abstract：:A double transgenic mouse expressing the amyloid precursor protein, bearing the Swedish mutations, and expressing tau protein containing three of the mutations present in frontotemporal dementia linked to chromosome 17 (FTDP-17), has been characterized. In the double transgenic mouse an increase in tau phosphorylation...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.09.029
更新日期：2005-01-01 00:00:00
abstract：:Hypocretin/orexin (Hcrt/Orx) and melanin concentrating hormone (MCH) are peptides contained in overlapping cell groups of the lateral hypothalamus and commonly involved in regulating sleep-wake states and energy balance, though likely in different ways. To see if these neurons are similarly or differentially modulated...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.10.032
更新日期：2005-01-01 00:00:00
abstract：:Autophagy plays an essential role in neurodevelopment, axonal guidance, neuropathic pain remission, and neuronal survival. Inhibiting the mammalian target of rapamycin (mTOR) signaling pathway can induce the occurrence of autophagy. In this study, we initially detected the effect of probucol on autophagy after spinal ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.05.019
更新日期：2016-08-04 00:00:00
abstract：:Intracellular recordings were made from submucous plexus neurons of the guinea-pig cecum maintained in vitro. Histamine (0.3-10 microM) produced a dose-dependent membrane depolarization (congruent to 13 mV with 3 microM) in about 28% of the cells tested; most of these cells showed a prominent calcium-activated potassi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90018-3
更新日期：1989-01-01 00:00:00
abstract：:To date, the neurochemical basis underlying the motor and cognitive deficits described in patients with multiple sclerosis (MS) is unclear. Since the neuropeptide somatostatin (SRIF) and the striatum have been implicated in movement control and implicit memory, the aim of this study was to analyze the striatal somatos...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.04.065
更新日期：2007-08-10 00:00:00
abstract：:Hippocampal neurogenesis and associated cognitive behaviors are regulated by a number of factors including stress, inflammation, and exercise. However, the interplay between these factors remains relatively unexplored, especially across the lifespan. In the current study, the effect of social isolation stress during t...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.09.020
更新日期：2017-12-04 00:00:00
abstract：:Studies demonstrated that chronic high-dose homocysteine administration induced learning and memory impairment in animals. Atractylenolide III (Aen-III), a neuroprotective constituent of Atractylodis macrocephalae Koidz, was isolated in our previous study. In this study, we investigated potential benefits of Aen-III i...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.01.060
更新日期：2015-04-02 00:00:00
abstract：:The distribution and staining pattern of gamma-aminobutyric acid immunoreactivity have been examined by both light and electron microscopy in the dorsal part of the lateral geniculate nucleus of three reptilian species: the turtle Chinemys reevesi, the lizard Ophisaurus apodus and the snake Vipera aspis. After perfusi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90254-y
更新日期：1992-01-01 00:00:00
abstract：:The aim of this study is to characterize calbindin-positive photoreceptors and their opsin content in the retina of nocturnal prosimians (Microcebus murinus), New World monkeys (Callithrix jacchus), Old World monkeys (Macaca fascicularis), and humans. To identify the calbindin and opsin content of cones, combined mult...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00327-5
更新日期：2002-01-01 00:00:00
abstract：:Recent advances in multiple areas of research have contributed to the identification of several pathophysiological factors underlying obsessive-compulsive disorder (OCD). In particular, the glutamate transporter gene SLC1A1 has been associated with the diagnosis of OCD. Immunological and infectious studies have report...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2009.10.043
更新日期：2010-01-20 00:00:00
abstract：:This study was performed using intracellular and multiunit extracellular recording techniques in order to characterize the role of nitric oxide in the afferent synaptic transmission of the vestibular system of the axolotl (Ambystoma tigrinum). Bath application of nitric oxide synthase inhibitors N(G)-nitro-L-arginine ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00587-x
更新日期：2001-01-01 00:00:00
abstract：:Activities of daily living require simultaneous and coordinated activation of trunk and upper-limb segments, which involves complex interlimb interaction within the central nervous system. Although many studies have reported associations between activity of trunk and limb muscles during functional tasks, evidence on c...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.10.011
更新日期：2020-12-15 00:00:00
abstract：:In several tauopathies such as Alzheimer's disease (AD), an increased incidence of seizures is observed. Tau, one of the major proteins implicated in AD pathology, is an important regulator of neural network excitability and might participate in the underlying epileptic cascade. However, the mechanisms underlying this...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.11.007
更新日期：2020-01-15 00:00:00
abstract：:Even simple behaviors of vertebrates are typically generated by the concerted action of large numbers of brain cells. However, the mechanisms by which groups of neurons work together as functional populations to guide behavior remain largely unknown. One of the major model systems for exploring these mechanisms has be...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.11.073
更新日期：2006-01-01 00:00:00
abstract：:We investigated immunohistochemically the cellular localization of multifunctional type II Ca2+/calmodulin-dependent protein kinase in the rat basal ganglia and intrastriatal grafts derived from fetal striatal primordia, in comparison with that of calcineurin, a reliable marker for striatal medium-sized spinous neuron...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90470-7
更新日期：1994-10-01 00:00:00
abstract：:Estradiol-induced plasticity involves changes in dendritic spine density and in the relative proportions of the different dendritic spine types that influence neurons and neural circuits. Such events affect brain structures that control the timing of neuroendocrine and behavioral processes, influencing both reproducti...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.04.019
更新日期：2015-07-09 00:00:00
abstract：:When the neural tracer Fluoro-Gold is used to retrogradely label a population of axotomized neurons, cellular labeling can persist in the axotomized nucleus even when Nissl staining indicates that the injured neurons have degenerated. In order to determine the identity of the labeled cells that remain, this study comb...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90096-7
更新日期：1991-01-01 00:00:00
abstract：:We investigated computer models of a single thalamocortical neuron to assess the interaction of intrinsic voltage-sensitive channels and cortical synaptic input in producing the range of oscillation frequencies observed in these cells in vivo. A morphologically detailed model with Hodgkin-Huxley-like ion channels demo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)83006-5
更新日期：1996-02-01 00:00:00
