Binding of dexamethasone by hepatic, intestine, and tailfin cytosol in Rana catesbeiana tadpoles during spontaneous and triiodothyronine-induced metamorphosis.

Abstract:

:The binding of the synthetic glucocorticoid dexamethasone (dex) to Rana catesbeiana tadpole liver, intestine, and tailfin cytosol during both spontaneous and triiodothyronine (T3)-induced metamorphosis has been examined. No change was observed in the dissociation constant (KD) in the liver or intestine during either spontaneous or T3-induced metamorphosis compared with liver and intestine cytosol from the frog. The binding capacity (N) in liver cytosol of premetamorphic tadpoles (14.33 x 10(-14) mol dex/mg protein) was not significantly different from that found during prometamorphosis (stage XVIII) (11.50 x 10(-14) mol dex/mg protein) and in the adult frog (19.24 x 10(-14) mol dex/mg protein). Following the onset of metamorphic climax, however, there were significant reductions in N in liver cytosol, reaching a nadir at stage XXIV (0.38 x 10(-14) mol dex/mg protein). Binding capacity in premetamorphic tadpole intestine (19.60 x 10(-14) mol dex/mg protein) was significantly reduced following premetamorphosis . Values did not return to premetamorphic values in the frog intestine (6.54 x 10(-14) mol dex/mg protein) as occurred in the frog liver, nor were values significantly reduced following the onset of metamorphic climax (10.43 x 10(-14) mol dex/mg protein) when compared with prometamorphosis (11.58 x 10(-14) mol dex/mg protein). The binding capacity in the tailfin cytosol did not deviate from premetamophic tadpole values (11.61 x 10(-14) mol dex/mg protein) through stage XXI (9.68 x 10(-14) mol dex/mg protein), the last stage in which sufficient tissue was available for analysis.

journal_name

Gen Comp Endocrinol

authors

Woody CJ,Jaffe RC

doi

10.1016/0016-6480(84)90173-4

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

194-202

issue

2

eissn

0016-6480

issn

1095-6840

journal_volume

54

pub_type

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