Abstract:
:Despite reports that Tris [tris (hydroxymethyl)aminomethane] affects platelets, it is often used to buffer suspending media. Human or rabbit platelets were washed and resuspended in Tyrode solution containing apyrase and 0.35% albumin. Addition of 15 mM Tris partially inhibited primary aggregation induced by 10 microM ADP and inhibited aggregation and release of 14C-serotonin from prelabelled platelets stimulated with low concentrations of thrombin (0.05-0.2 U/mL), or collagen. Platelets resuspended in 15 mM Tris, 0.15 M NaCl, 0.35% albumin, pH 7.5, did not aggregate in response to 10 microM ADP whereas platelets in Tyrode-albumin aggregated extensively. Ca2+ (5 mM) did not overcome the inhibition of thrombin-induced aggregation. Tris (15 or 1.5 mM) potentiated aggregation and release induced by sodium arachidonate (20-50 microM) or the ionophore A23187 (0.6-1 microM). Pretreatment of platelets with aspirin did not prevent potentiation by A23187, indicating that it is not mediated through activation of the arachidonate pathway. The inhibitory and potentiating effects of Tris are similar to those of amino sugars, lysine, arginine and primary amines such as methylamine and cadaverine, and may represent general effects of amines on platelets. Potentiation of the effects of some aggregating agents and inhibition of others re-emphasizes the concept that there are several different mechanisms through which aggregation can occur. Tris-based buffers are unsuitable for platelet suspending media and their use as solvents for aggregating agents or inhibitors should be limited.
journal_name
Thromb Haemostjournal_title
Thrombosis and haemostasisauthors
Packham MA,Guccione MA,Nina M,Kinlough-Rathbone RL,Mustard JFsubject
Has Abstractpub_date
1984-04-30 00:00:00pages
140-4issue
2eissn
0340-6245issn
2567-689Xjournal_volume
51pub_type
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
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journal_title:Thrombosis and haemostasis
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更新日期:1994-12-01 00:00:00
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journal_title:Thrombosis and haemostasis
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doi:
更新日期:1983-06-28 00:00:00
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更新日期:2009-08-01 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
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journal_title:Thrombosis and haemostasis
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更新日期:1999-12-01 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
更新日期:1990-02-19 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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journal_title:Thrombosis and haemostasis
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doi:
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pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
更新日期:1976-08-31 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章,评审
doi:
更新日期:1978-10-31 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:10.1160/TH10-04-0216
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
更新日期:1976-08-31 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
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更新日期:2000-06-01 00:00:00
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journal_title:Thrombosis and haemostasis
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1160/TH04-03-0154
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