Abstract:
:We used a murine model to determine whether chronic corticosteroid therapy has uniform effects on bacterial clearance processes in different tissues. After a 2-week regimen of oral prednisolone, Swiss Webster mice were challenged with Pseudomonas aeruginosa strain PAO1 or virulent derivatives of PAO1 (IP-8 and IT-10). Chronic corticosteroid therapy delayed the clearance of strain PAO1 from the peritoneal cavity. However, steroid treatment did not reduce the neutrophil influx, did not reduce the in vivo phagocytic capacity of neutrophils, and did not alter bactericidal activity of peritoneal exudate cells in vitro. Virulent isolates (IP-8 and IT-10) replicated in the peritoneal cavity in steroid-treated mice even though the neutrophil influx was similar to control mice. In contrast to the abnormal peritoneal clearance, all strains were rapidly cleared from the lungs of control and steroid-treated mice after aerosol challenge. Neutrophil influx into bronchoalveolar spaces was greater in steroid-treated mice than in control mice. All mice (control and steroid treated) survived these challenges, except for some steroid-treated mice infected intraperitoneally with IP-8. These results demonstrate that chronic steroid therapy alters bacterial clearance processes on the peritoneal surfaces to a greater extent than in the lower respiratory tract. The explanation for this altered clearance in the peritoneum is unclear, but cannot be explained by reductions in neutrophil migration to inflammatory stimuli. Therefore, the infectious risk associated with chronic corticosteroid therapy appears to depend on both the tissue and the virulence of the bacterial strain and may reflect alterations in clearance processes other than neutrophil migration.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nugent KM,Cox CD,Pesanti ELdoi
10.1128/IAI.43.3.901-905.1984subject
Has Abstractpub_date
1984-03-01 00:00:00pages
901-5issue
3eissn
0019-9567issn
1098-5522journal_volume
43pub_type
杂志文章abstract::Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.318-326.1982
更新日期:1982-07-01 00:00:00
abstract::A major virulence factor of group A streptococci is M protein, a surface-exposed fibrillar molecule of which there exist more than 80 distinct serological types. Antigenic variability resides largely in the amino-terminal region of M protein, whereas the carboxy-terminal half of the molecule is highly conserved among ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2666-2672.1988
更新日期:1988-10-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
abstract::We investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (PBMCs) cocultured with adult liver flukes (Opisthorchis viverrini) for 6 to 24 h. PBMC-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytok...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00901-13
更新日期:2014-05-01 00:00:00
abstract::In this study we investigated the mucosal and systemic responses to two T-cell-independent polysaccharides, a serogroup f polysaccharide (formed of rhamnose glucose polymers [RGPs]) from Streptococcus mutans OMZ 175 and a mannan from Saccharomyces cerevisiae, covalently conjugated either to a linear peptide (peptide 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2645-2651.1995
更新日期:1995-07-01 00:00:00
abstract::We studied seven strains of group G streptococci isolated from clinically severe bacteremic infections in six intravenous drug abusers. These group G strains multiplied luxuriantly in fresh human blood. On electron microscopy, they exhibited surface fibrillae similar to those observed in M-protein-rich group A strepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.753-757.1987
更新日期:1987-03-01 00:00:00
abstract::We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3623-3627.2003
更新日期:2003-06-01 00:00:00
abstract::Our previous studies identified two iron-regulated cytoplasmic membrane proteins of 32 and 36 kDa expressed by both Staphylococcus epidermidis and Staphylococcus aureus. In this study we show by Triton X-114 phase partitioning and tritiated palmitic acid labelling that these proteins are lipoproteins which are anchore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3767-3774.1998
更新日期:1998-08-01 00:00:00
abstract::In this study, native cells of Streptococcus mutans VA-29R and Streptococcus rattus FA-1 displayed significantly higher aminopeptidase activity than did cells of Streptococcus cricetus AHT or Streptococcus sobrinus 6715 toward the nitroanilide derivatives of leucine, alanine, methionine, arginine, and lysine. These di...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.182-186.1993
更新日期:1993-01-01 00:00:00
abstract::Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.4.431-440.1971
更新日期:1971-10-01 00:00:00
abstract::We have previously described a new system for the delivery of recombinant antigens in live Salmonella vaccines as genetic fusions to the C terminus of fragment C of tetanus toxin (TetC) driven by the anaerobically inducible nirB promoter. It has been reported that preimmunization with tetanus toxoid (TT) can suppress ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
更新日期:1995-07-01 00:00:00
abstract::The mucosal and systemic immune responses after primary and booster immunizations with two attenuated live oral vaccine strains derived from a noninvasive (Vibrio cholerae) and an invasive (Salmonella typhi) enteric pathogen were comparatively evaluated. Vaccination with S. typhi Ty21a elicited antibody-secreting cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3680-3685.1999
更新日期:1999-07-01 00:00:00
abstract::Pneumocystis carinii is an ascomycete phylogenetically related to Schizosaccharomyces pombe. Little is known about gene regulation in P. carinii. The removal of introns from pre-mRNA requires spliceosomal recognition of the intron-exon boundary. In S. pombe and higher eukaryotes, this boundary and a branch site within...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6157-6160.1999
更新日期:1999-11-01 00:00:00
abstract::A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4011-4016.1997
更新日期:1997-10-01 00:00:00
abstract::Naegleria fowleri produces fatal meningoencephalitis in humans and in experimentally infected laboratory animals. The course of the disease in mice is dependent upon the infecting dose of amoebae, route of inoculation, and prior exposure to Naegleria antigens. DUB/ICR mice were immunized by various routes and antigen ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.817-820.1977
更新日期:1977-06-01 00:00:00
abstract::By using in vitro assays, a group of related, filamentous gram-negative bacteria isolated from subgingival plaque deposits of patients with periodontal disease were found to colonize intact teeth. Tentatively identified as members of the genus Cytophaga, these isolates exhibited a preference for colonizing the cementu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.15-18.1979
更新日期:1979-10-01 00:00:00
abstract::Encapsulation of soluble protein antigens in liposomes was previously shown to result in processing of antigen via the major histocompatibility complex class I pathway, as evidenced by costaining of the trans-Golgi region of murine bone marrow-derived macrophages (BMs) by fluorophore-labeled liposomal antigen and by a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2859-2865.1998
更新日期:1998-06-01 00:00:00
abstract::An escape variant of Borrelia burgdorferi, selected with a monoclonal antibody to OspB, expressed a truncated form of OspB, the result of point mutations in the ospB gene leading to a premature termination codon. A single amino acid position in the C terminus of OspB was critical for monoclonal antibody recognition. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.303-307.1994
更新日期:1994-01-01 00:00:00
abstract::Whole cells, morphological components, and various extracts of Candida albicans were tested for toxicity by methods involving biological activities ordinarily used to characterize bacterial endotoxin. Fungal preparations exerted several of these activities, but only at much higher dose levels than those required for b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.616-627.1972
更新日期:1972-10-01 00:00:00
abstract::Shigella flexneri 2a is an invasive enteric pathogen that may produce diarrhea when ingested by human beings and subhuman primates. We have previously shown that shigella diarrhea correlates with water and electrolyte transport abnormalities in the jejunum and colon. Dysentery alone is associated only with colonic tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.368-371.1976
更新日期:1976-08-01 00:00:00
abstract::Neisseria gonorrhoeae, the causative agent of the sexually transmitted infection (STI) gonorrhea, is a growing public health threat for which a vaccine is urgently needed. We characterized the functional role of the gonococcal MetQ protein, which is the methionine binding component of an ABC transporter system, and as...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00898-16
更新日期:2017-01-26 00:00:00
abstract::Studies were carried out to determine whether the in vitro capacity of adherent peritoneal cells to replicate herpes simplex virus type 1 (HSV-1) might correlate with the in vivo susceptibility of mice genetically resistant, moderately susceptible, or very susceptible to HSV-1 infection. Unstimulated and proteose pept...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.432-437.1979
更新日期:1979-02-01 00:00:00
abstract::Salmonella typhimurium SH5014 porins induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin 1 alpha (IL-1 alpha), and IL-6 by human monocytes and of gamma interferon (IFN-gamma) and IL-4 by human lymphocytes. Porins at 1 microgram/ml induce the greatest release of TNF-alpha, IL-1 alpha, and IL-6 by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.155-161.1993
更新日期:1993-01-01 00:00:00
abstract::Antibody rises to various virus subcomponents were measured by enzyme-linked immunosorbent assay in the paired sera of volunteers experimentally infected with human coronavirus 229E group viruses. Most of the antibody made during infection was directed against the virus surface projections, with only small amounts of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.845-849.1981
更新日期:1981-03-01 00:00:00
abstract::We developed a human inhibition monoclonal enzyme-linked immunosorbent assay (HIMELISA) to investigate the human immune response to the lipooligosaccharides (LOS) of Neisseria meningitidis. Monoclonal antibodies (MAb) were used to define seven epitopes on four LOS molecules of a meningococcal strain (126E) previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2204-2213.1990
更新日期:1990-07-01 00:00:00
abstract::The role of a plasmid in the virulence activity of an enteropathogenic Escherichia coli (EPEC) strain belonging to serotype 0111:NM was examined. EPEC strain B171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pYR111, and exhibits localized adher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2052-2056.1987
更新日期:1987-09-01 00:00:00
abstract::Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.711-719.1978
更新日期:1978-02-01 00:00:00
abstract::There is an emerging paradigm shift from coronary heart disease having a purely hereditary and nutritional causation to possibly having an infectious etiology. Recent epidemiological studies have shown a correlation between periodontal disease and coronary heart disease. However, to date, there is minimal information ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5792-5798.1999
更新日期:1999-11-01 00:00:00
abstract::Thirty percent of Streptococcus pneumoniae isolates contain pilus islet 1, coding for a pilus composed of the backbone subunit RrgB and two ancillary proteins, RrgA and RrgC. RrgA is the major determinant of in vitro adhesion associated with pilus 1, is protective in vivo in mouse models, and exists in two variants (c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00601-10
更新日期:2010-12-01 00:00:00
abstract::Various features of salmonellosis were examined in a burned-mouse model. In this model, which uses an outbred mouse strain, a challenge dose of ca. 100 CFU with any of several strains of Salmonella typhimurium caused a fatal infection. A variety of mutated strains attenuated for virulence in Salmonella-susceptible par...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2842-2846.1989
更新日期:1989-09-01 00:00:00