Formation of proinsulin by immobilized Bacillus subtilis.

Abstract:

:There has been an increasing interest in the use of immobilized cells for the production of pharmaceuticals as well as for products such as high fructose syrup or ethanol. Some of these compounds are now produced on an industrial scale whereby the cells are used in a resting or growing state or in a nonviable form as natural carriers of the enzyme(s) involved in the synthesis. The advantages of immobilized cell technology should also apply to microorganisms modified by recombinant DNA techniques to produce a variety of eukaryotic proteins such as hormones. We describe here the properties of immobilized Bacillus subtilis cells carrying plasmids encoding rat proinsulin. Cell proliferation normally coupled to DNA replication is undesirable in immobilized cell systems as "clogging' of the system occurs due to cells growing outside the beads. Therefore, different ways were investigated to inhibit cell division while allowing continued protein synthesis. We found that the addition of certain antibiotics in the growth medium, such as novobiocin which inhibits DNA replication, fulfills these requirements, allowing proinsulin synthesis and excretion to take place over a period of several days.

journal_name

Nature

journal_title

Nature

authors

Mosbach K,Birnbaum S,Hardy K,Davies J,Bülow L

doi

10.1038/302543a0

subject

Has Abstract

pub_date

1983-04-07 00:00:00

pages

543-5

issue

5908

eissn

0028-0836

issn

1476-4687

journal_volume

302

pub_type

杂志文章

相关文献

NATURE文献大全
  • Myristylation of picornavirus capsid protein VP4 and its structural significance.

    abstract::We have obtained evidence that poliovirus and other picornavirus particles are specifically modified by having myristic acid covalently bound to a capsid protein. The electron density map of poliovirus confirms the position of the myristate molecule and defines its location in the virus particle. Analogies with other ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/327482a0

    authors: Chow M,Newman JF,Filman D,Hogle JM,Rowlands DJ,Brown F

    更新日期:1987-06-11 00:00:00

  • B220: a B cell-specific member of th T200 glycoprotein family.

    abstract::T200, a major cell-surface glycoprotein on lymphoid cells, exists in several forms with different electrophoretic mobilities. These forms have been correlated with different classes of lymphoid cell. The smaller forms, with molecular weights (MWs) of congruent to 170,000 and 180,000 are found predominantly on T cells ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/289681a0

    authors: Coffman RL,Weissman IL

    更新日期:1981-02-19 00:00:00

  • In situ observation of incompressible Mott-insulating domains in ultracold atomic gases.

    abstract::The observation of the superfluid to Mott insulator phase transition of ultracold atoms in optical lattices was an enabling discovery in experimental many-body physics, providing the first tangible example of a quantum phase transition (one that occurs even at zero temperature) in an ultracold atomic gas. For a trappe...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature08244

    authors: Gemelke N,Zhang X,Hung CL,Chin C

    更新日期:2009-08-20 00:00:00

  • A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans.

    abstract::How left/right functional asymmetry is layered on top of an anatomically symmetrical nervous system is poorly understood. In the nematode Caenorhabditis elegans, two morphologically bilateral taste receptor neurons, ASE left (ASEL) and ASE right (ASER), display a left/right asymmetrical expression pattern of putative ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02255

    authors: Johnston RJ,Hobert O

    更新日期:2003-12-18 00:00:00

  • Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

    abstract::Ageing is characterized by the development of persistent pro-inflammatory responses that contribute to atherosclerosis, metabolic syndrome, cancer and frailty1-3. The ageing brain is also vulnerable to inflammation, as demonstrated by the high prevalence of age-associated cognitive decline and Alzheimer's disease4-6. ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-03160-0

    authors: Minhas PS,Latif-Hernandez A,McReynolds MR,Durairaj AS,Wang Q,Rubin A,Joshi AU,He JQ,Gauba E,Liu L,Wang C,Linde M,Sugiura Y,Moon PK,Majeti R,Suematsu M,Mochly-Rosen D,Weissman IL,Longo FM,Rabinowitz JD,Andreasson K

    更新日期:2021-01-20 00:00:00

  • The gut microbiota is associated with immune cell dynamics in humans.

    abstract::The gut microbiota influences development1-3 and homeostasis4-7 of the mammalian immune system, and is associated with human inflammatory8 and immune diseases9,10 as well as responses to immunotherapy11-14. Nevertheless, our understanding of how gut bacteria modulate the immune system remains limited, particularly in ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-2971-8

    authors: Schluter J,Peled JU,Taylor BP,Markey KA,Smith M,Taur Y,Niehus R,Staffas A,Dai A,Fontana E,Amoretti LA,Wright RJ,Morjaria S,Fenelus M,Pessin MS,Chao NJ,Lew M,Bohannon L,Bush A,Sung AD,Hohl TM,Perales MA,van den

    更新日期:2020-12-01 00:00:00

  • Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression.

    abstract::Reactive oxygen species are involved in many cellular metabolic and signalling processes and are thought to have a role in disease, particularly in carcinogenesis and ageing. We have generated mice with targeted inactivation of Prdx1, a member of the peroxiredoxin family of antioxidant enzymes. Here we show that mice ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature01819

    authors: Neumann CA,Krause DS,Carman CV,Das S,Dubey DP,Abraham JL,Bronson RT,Fujiwara Y,Orkin SH,Van Etten RA

    更新日期:2003-07-31 00:00:00

  • Two dynamically distinct circuits drive inhibition in the sensory thalamus.

    abstract::Most sensory information destined for the neocortex is relayed through the thalamus, where considerable transformation occurs1,2. One means of transformation involves interactions between excitatory thalamocortical neurons that carry data to the cortex and inhibitory neurons of the thalamic reticular nucleus (TRN) tha...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-2512-5

    authors: Martinez-Garcia RI,Voelcker B,Zaltsman JB,Patrick SL,Stevens TR,Connors BW,Cruikshank SJ

    更新日期:2020-07-01 00:00:00

  • Nuclear reprogramming and pluripotency.

    abstract::The cloning of mammals from differentiated donor cells has refuted the old dogma that development is an irreversible process. It has demonstrated that the oocyte can reprogramme an adult nucleus into an embryonic state that can direct development of a new organism. The prospect of deriving patient-specific embryonic s...

    journal_title:Nature

    pub_type: 杂志文章,评审

    doi:10.1038/nature04955

    authors: Hochedlinger K,Jaenisch R

    更新日期:2006-06-29 00:00:00

  • A massive star origin for an unusual helium-rich supernova in an elliptical galaxy.

    abstract::The unusual helium-rich (type Ib) supernova SN 2005E is distinguished from all supernovae hitherto observed by its faint and rapidly fading light curve, prominent calcium lines in late-phase spectra and lack of any mark of recent star formation near the supernova location. These properties are claimed to be explained ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature09055

    authors: Kawabata KS,Maeda K,Nomoto K,Taubenberger S,Tanaka M,Deng J,Pian E,Hattori T,Itagaki K

    更新日期:2010-05-20 00:00:00

  • Astrocytes present myelin basic protein to encephalitogenic T-cell lines.

    abstract::Astrocyte proliferation and perivascular lymphocyte infiltration are conspicuous among the cellular changes in the active brain lesions of multiple sclerosis patients. Recent observations have indicated that most of the perivascular lymphocytes are T cells which may be actively involved in the generation of the brain ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/307273a0

    authors: Fontana A,Fierz W,Wekerle H

    更新日期:1984-01-19 00:00:00

  • The chemical structure of the Hawaiian mantle plume.

    abstract::The Hawaiian-Emperor volcanic island and seamount chain is usually attributed to a hot mantle plume, located beneath the Pacific lithosphere, that delivers material sourced from deep in the mantle to the surface. The shield volcanoes of the Hawaiian islands are distributed in two curvilinear, parallel trends (termed '...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature03907

    authors: Ren ZY,Ingle S,Takahashi E,Hirano N,Hirata T

    更新日期:2005-08-11 00:00:00

  • Potassium conductances in hippocampal neurons blocked by excitatory amino-acid transmitters.

    abstract::Excitatory amino acids mediate fast synaptic transmission in the central nervous system through the activation of at least three distinct ionotropic receptors: N-methyl-D-aspartate (NMDA), the alpha-amino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA)/quisqualate (QUIS) and the kainate subtypes (for reviews, see ref...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/347765a0

    authors: Charpak S,Gähwiler BH,Do KQ,Knöpfel T

    更新日期:1990-10-25 00:00:00

  • Reconstitution of active dimeric ribulose bisphosphate carboxylase from an unfoleded state depends on two chaperonin proteins and Mg-ATP.

    abstract::In vitro reconstitution of active ribulose bisphosphate carboxylase (Rubisco) from unfolded polypeptides is facilitated by the molecular chaperones: chaperonin-60 from Escherichia coli (groEL), yeast mitochondria (hsp60) or chloroplasts (Rubisco sub-unit-binding protein), together with chaperonin-10 from E. coli (groE...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/342884a0

    authors: Goloubinoff P,Christeller JT,Gatenby AA,Lorimer GH

    更新日期:1989-12-21 00:00:00

  • Elevation alters ecosystem properties across temperate treelines globally.

    abstract::Temperature is a primary driver of the distribution of biodiversity as well as of ecosystem boundaries. Declining temperature with increasing elevation in montane systems has long been recognized as a major factor shaping plant community biodiversity, metabolic processes, and ecosystem dynamics. Elevational gradients,...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature21027

    authors: Mayor JR,Sanders NJ,Classen AT,Bardgett RD,Clément JC,Fajardo A,Lavorel S,Sundqvist MK,Bahn M,Chisholm C,Cieraad E,Gedalof Z,Grigulis K,Kudo G,Oberski DL,Wardle DA

    更新日期:2017-02-02 00:00:00

  • A developmental coordinate of pluripotency among mice, monkeys and humans.

    abstract::The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of pluripotent stem cells in vitro. To explore the ontogeny of human and primate pluripotency, here we perform comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cynomolgus monkeys (Macaca fasciculari...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature19096

    authors: Nakamura T,Okamoto I,Sasaki K,Yabuta Y,Iwatani C,Tsuchiya H,Seita Y,Nakamura S,Yamamoto T,Saitou M

    更新日期:2016-09-01 00:00:00

  • Impaired T(H)17 cell differentiation in subjects with autosomal dominant hyper-IgE syndrome.

    abstract::The autosomal dominant hyper-IgE syndrome (HIES, 'Job's syndrome') is characterized by recurrent and often severe pulmonary infections, pneumatoceles, eczema, staphylococcal abscesses, mucocutaneous candidiasis, and abnormalities of bone and connective tissue. Mutations presumed to underlie HIES have recently been ide...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature06764

    authors: Milner JD,Brenchley JM,Laurence A,Freeman AF,Hill BJ,Elias KM,Kanno Y,Spalding C,Elloumi HZ,Paulson ML,Davis J,Hsu A,Asher AI,O'Shea J,Holland SM,Paul WE,Douek DC

    更新日期:2008-04-10 00:00:00

  • Atomic structure of the eukaryotic intramembrane RAS methyltransferase ICMT.

    abstract::The maturation of RAS GTPases and approximately 200 other cellular CAAX proteins involves three enzymatic steps: addition of a farnesyl or geranylgeranyl prenyl lipid to the cysteine (C) in the C-terminal CAAX motif, proteolytic cleavage of the AAX residues and methylation of the exposed prenylcysteine residue at its ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature25439

    authors: Diver MM,Pedi L,Koide A,Koide S,Long SB

    更新日期:2018-01-25 00:00:00

  • Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.

    abstract::A single U leads to G transversion in the 3' consensus sequence AAUAAA of the adenovirus early region 1A gene was constructed and the effect of this mutation on processing of the 3' end of the nuclear early region 1A RNAs was analysed. The results demonstrate that the intact AAUAAA is not required for RNA polyadenylat...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/305600a0

    authors: Montell C,Fisher EF,Caruthers MH,Berk AJ

    更新日期:1983-10-13 00:00:00

  • Reconstitution of a microtubule plus-end tracking system in vitro.

    abstract::The microtubule cytoskeleton is essential to cell morphogenesis. Growing microtubule plus ends have emerged as dynamic regulatory sites in which specialized proteins, called plus-end-binding proteins (+TIPs), bind and regulate the proper functioning of microtubules. However, the molecular mechanism of plus-end associa...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature06386

    authors: Bieling P,Laan L,Schek H,Munteanu EL,Sandblad L,Dogterom M,Brunner D,Surrey T

    更新日期:2007-12-13 00:00:00

  • Poly(dA).poly(dT) is a B-type double helix with a distinctively narrow minor groove.

    abstract::The structure of poly(dA).poly(dT) currently arouses great interest, mainly because dAn.dTn stretches are associated with considerable DNA bending. Until recently the heteronomous DNA described by Arnott et al., with the poly(dA) and poly(dT) chains in A and B conformations respectively, was the only detailed model of...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/325821a0

    authors: Alexeev DG,Lipanov AA,Skuratovskii IYa

    更新日期:1987-02-26 00:00:00

  • Correlation of DNA exonic regions with protein structural units in haemoglobin.

    abstract::The discovery of intervening sequences (introns) in DNA led Gilbert and Tonegawa to suggest that a new protein could have been produced by bringing together certain segments of pre-existing ones. However, Blake argued that if DNA was so organized that coding sequences (exons) correspond to structural as well as functi...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/291090a0

    authors: Go M

    更新日期:1981-05-07 00:00:00

  • Signal clipping by the rod output synapse.

    abstract::The properties of synapses between retinal neurons make an essential contribution to early visual processing. Light produces a graded hyperpolarization in photoreceptors, up to 25 mV in amplitude, and it is conventionally assumed that all of this response range is available for coding visual information. We report her...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/328522a0

    authors: Attwell D,Borges S,Wu SM,Wilson M

    更新日期:1987-08-06 00:00:00

  • A point mutation in the A gamma-globin gene promoter in Greek hereditary persistence of fetal haemoglobin.

    abstract::Hereditary persistance of fetal haemoglobin (HPFH) is a benign condition characterized by the production in adulthood of more than 1% fetal haemoglobin (HbF, alpha 2 gamma 2) in the absence of erythropoietic stress. Several genetic types have been discerned based on the level of HbF produced, the relative contribution...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/313325a0

    authors: Collins FS,Metherall JE,Yamakawa M,Pan J,Weissman SM,Forget BG

    更新日期:1985-01-24 00:00:00

  • Cooperation and competition in pathogenic bacteria.

    abstract::Explaining altruistic cooperation is one of the greatest challenges for evolutionary biology. One solution to this problem is if costly cooperative behaviours are directed towards relatives. This idea of kin selection has been hugely influential and applied widely from microorganisms to vertebrates. However, a problem...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02744

    authors: Griffin AS,West SA,Buckling A

    更新日期:2004-08-26 00:00:00

  • Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å.

    abstract::Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 Å. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature09913

    authors: Umena Y,Kawakami K,Shen JR,Kamiya N

    更新日期:2011-05-05 00:00:00

  • Adenylyl cyclases and the interaction between calcium and cAMP signalling.

    abstract::Adenylyl cyclase is the prototypical second messenger generator. Nearly all of the eight cloned adenylyl cyclases are regulated by one or other arm of the phospholipase C pathway. Functional and ultrastructural investigations have shown that adenylyl cyclases are intimately associated with sites of calcium ion entry i...

    journal_title:Nature

    pub_type: 杂志文章,评审

    doi:10.1038/374421a0

    authors: Cooper DM,Mons N,Karpen JW

    更新日期:1995-03-30 00:00:00

  • An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay.

    abstract::The specification of both the germ line and abdomen in Drosophila depends on the localization of oskar messenger RNA to the posterior of the oocyte. This localization requires several trans-acting factors, including Barentsz and the Mago-Y14 heterodimer, which assemble with oskar mRNA into ribonucleoprotein particles ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02351

    authors: Palacios IM,Gatfield D,St Johnston D,Izaurralde E

    更新日期:2004-02-19 00:00:00

  • A new hominid from the Upper Miocene of Chad, Central Africa.

    abstract::The search for the earliest fossil evidence of the human lineage has been concentrated in East Africa. Here we report the discovery of six hominid specimens from Chad, central Africa, 2,500 km from the East African Rift Valley. The fossils include a nearly complete cranium and fragmentary lower jaws. The associated fa...

    journal_title:Nature

    pub_type: 历史文章,杂志文章

    doi:10.1038/nature00879

    authors: Brunet M,Guy F,Pilbeam D,Mackaye HT,Likius A,Ahounta D,Beauvilain A,Blondel C,Bocherens H,Boisserie JR,De Bonis L,Coppens Y,Dejax J,Denys C,Duringer P,Eisenmann V,Fanone G,Fronty P,Geraads D,Lehmann T,Lihoreau F,

    更新日期:2002-07-11 00:00:00

  • Cytoplasmic chromatin triggers inflammation in senescence and cancer.

    abstract::Chromatin is traditionally viewed as a nuclear entity that regulates gene expression and silencing. However, we recently discovered the presence of cytoplasmic chromatin fragments that pinch off from intact nuclei of primary cells during senescence, a form of terminal cell-cycle arrest associated with pro-inflammatory...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature24050

    authors: Dou Z,Ghosh K,Vizioli MG,Zhu J,Sen P,Wangensteen KJ,Simithy J,Lan Y,Lin Y,Zhou Z,Capell BC,Xu C,Xu M,Kieckhaefer JE,Jiang T,Shoshkes-Carmel M,Tanim KMAA,Barber GN,Seykora JT,Millar SE,Kaestner KH,Garcia BA,Ada

    更新日期:2017-10-19 00:00:00