Colony assay using collagen gel: a better technique for studying the cell morphology of colonies.

Abstract:

:Collagen gel was used as a substance for the assay of human granulocyte-macrophage colony-forming units (CFUc). The number of colonies formed in collagen gel was consistently higher than that in a conventional soft-agar system. In six out of eight experiments, the difference was statistically significant. Whole permanent preparations of the plates were made easily and were stained by Giemsa solution with minimum background staining. Preservation of cellular morphology in the preparations was excellent, and neutrophil, macrophage, and eosinophil colonies could be differentiated by their nuclear shape, cytoplasmic color, and specific granules.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Suda T,Nemoto K,Izumi T,Miyawaki S,Maekawa T

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

245-9

issue

4

eissn

0301-472X

issn

1873-2399

journal_volume

12

pub_type

杂志文章
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    authors: Coggle JE,Gordon MY

    更新日期:1975-06-01 00:00:00

  • Immunological properties of bone marrow microenvironment 1 year after allogeneic hematopoietic stem cell transplantation.

    abstract::Regeneration of the bone marrow microenvironment after transplantation of allogeneic hematopoietic stem cells is poorly explored. The goal of our study was to investigate this process focusing on immunologic factors: concentrations of selected cytokines, expression of immunosuppressive proteins CD47 and CD274 on hemat...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2016.08.001

    authors: Ciomber A,Mitrus I,Fidyk W,Smagur A,Chwieduk A,Glowala-Kosinska M,Czerw T,Sobczyk-Kruszelnicka M,Mendrek W,Sadus-Wojciechowska M,Najda J,Holowiecki J,Giebel S

    更新日期:2016-12-01 00:00:00

  • Anti-CD117 immunotherapy to eliminate hematopoietic and leukemia stem cells.

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    doi:10.1016/j.exphem.2021.01.003

    authors: Russkamp NF,Myburgh R,Kiefer JD,Neri D,Manz MG

    更新日期:2021-01-20 00:00:00

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Shadduck RK,Waheed A,Mangan KF,Rosenfeld CS

    更新日期:1993-04-01 00:00:00

  • Cell kinetics of GM-CFC in the steady state.

    abstract::The kinetics of cell turnover for myeloid/monocyte cells that form colonies in agar (GM-CFC) were measured through the progressive increase in their sensitivity to 313-nm light during a period of cell labeling with BrdCyd. Two components of cell killing with distinctly separate labeling kinetics revealed both the pres...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Hagan MP,MacVittie TJ,Dodgen DP

    更新日期:1985-07-01 00:00:00

  • Proliferation of lymphoid precursor cells in the bone marrow of patients with various disorders of the immune system.

    abstract::In the human bone marrow the nuclear enzyme terminal deoxynucleotidyl transferase (TdT) is expressed by cells during early stages of lymphocyte differentiation. In order to investigate a possible regulation of lymphopoiesis at this level of differentiation, the relative frequency and the in vitro 3H-thymidine labeling...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Hirt A,Morell A,Frei H,Imbach P,Wagner HP

    更新日期:1988-01-01 00:00:00

  • Human adipose tissue-derived mesenchymal stem cells facilitate the immunosuppressive effect of cyclosporin A on T lymphocytes through Jagged-1-mediated inhibition of NF-κB signaling.

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2010.10.009

    authors: Shi D,Liao L,Zhang B,Liu R,Dou X,Li J,Zhu X,Yu L,Chen D,Zhao RC

    更新日期:2011-02-01 00:00:00

  • GATA transcription in a small rhodamine 123(low)CD34(+) subpopulation of a peripheral blood-derived CD34(-)CD105(+) mesenchymal cell line.

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(02)00865-2

    authors: Conrad C,Gottgens B,Kinston S,Ellwart J,Huss R

    更新日期:2002-08-01 00:00:00

  • TGF-beta1 drives and accelerates erythroid differentiation in the epo-dependent UT-7 cell line even in the absence of erythropoietin.

    abstract:OBJECTIVE:TGF-beta1 is a powerful inhibitor of erythropoiesis. However, its mechanisms of action are not fully elucidated yet at the cellular level. In this work we have studied the effects of TGF-beta on UT-7 cell survival, proliferation and differentiation. MATERIALS AND METHODS:UT-7 cell line is strictly dependent ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(99)00155-1

    authors: Zermati Y,Varet B,Hermine O

    更新日期:2000-03-01 00:00:00

  • A new form of Filgrastim with sustained duration in vivo and enhanced ability to mobilize PBPC in both mice and humans.

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(99)00112-5

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    更新日期:1999-12-01 00:00:00

  • Induction of murine peritoneal macrophage colony-forming cells by peritoneal administration of macrophage inflammatory protein-1 alpha.

    abstract::Peritoneal injection of thioglycollate medium (TM) to mice results in a dramatic increase in total number of peritoneal macrophages within 48 to 72 hours. Unlike resident macrophages, a fraction (10 to 20%) of these newly arrived young macrophages, designated as macrophage colony-forming cells (M-CFC), are highly prol...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Chen B,Chou TH,Sensenbrenner L

    更新日期:1993-11-01 00:00:00

  • Improvement of erythroid toxicity by lentinan and erythropoietin in mice treated with chemotherapeutic agents.

    abstract::Lentinan, an antitumor polysaccharide, has been assessed for its potential in vivo to augment erythroid progenitor cells and protect them from the cytotoxic effects of antitumor chemotherapeutics. Lentinan augmented the level of burst-forming units-erythroid (BFU-E) and accelerated the recovery of the reduced number o...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Takatsuki F,Miyasaka Y,Kikuchi T,Suzuki M,Hamuro J

    更新日期:1996-02-01 00:00:00

  • Efficient gene transfer into hematopoietic cells by a retargeting adenoviral vector system with a chimeric fiber of adenovirus serotype 5 and 11p.

    abstract:OBJECTIVE:Adenoviral vectors (Ad) were widely used in gene therapy and study of gene function, but the commonly used serotype 5 adenovirus-based vectors (Ad5) could poorly transduce hematopoietic cells because of low expression of viral receptors on these cells. To overcome this limitation, we developed a retargeting a...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2006.05.005

    authors: Lu ZZ,Ni F,Hu ZB,Wang L,Wang H,Zhang QW,Huang WR,Wu CT,Wang LS

    更新日期:2006-09-01 00:00:00

  • Thyroid hormones enhance hypoxia-induced erythropoietin production in vitro.

    abstract::Effects of thyroid hormones on the production of erythropoietin (Epo) were investigated in isolated perfused rat kidneys and in the human hepatoma cell line, HepG2. Epo protein was measured by radioimmunoassay. L-triiodothyronine and L-thyroxine stimulated hypoxia-induced Epo formation both in the kidney and in HepG2 ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Fandrey J,Pagel H,Frede S,Wolff M,Jelkmann W

    更新日期:1994-03-01 00:00:00

  • Robust conversion of marrow cells to skeletal muscle with formation of marrow-derived muscle cell colonies: a multifactorial process.

    abstract:OBJECTIVE:Murine marrow cells are capable of repopulating skeletal muscle fibers. A point of concern has been the "robustness" of such conversions. We have investigated the impact of type of cell delivery, muscle injury, nature of delivered cell, and stem cell mobilizations on marrow-to-muscle conversion. METHODS:We t...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2004.02.007

    authors: Abedi M,Greer DA,Colvin GA,Demers DA,Dooner MS,Harpel JA,Weier HU,Lambert JF,Quesenberry PJ

    更新日期:2004-05-01 00:00:00

  • The interaction of erythropoietin with fetal liver cells. II. Inhibition of the erythropoietin effect by interferon.

    abstract::To investigate the effect of interferon on the proliferation of normal erythroid precursor cells, various interferon preparations were added to mouse fetal liver cells cultured in a liquid microculture system. The effect of interferon was studied using cellular tritiated thymidine incorporation both in the presence an...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Smith KA,Fredrickson TN,Mobraaten LE,DeMaeyer E

    更新日期:1977-09-01 00:00:00

  • How hematopoietic stem/progenitors and their niche sense and respond to infectious stress.

    abstract::Hematopoietic stem/progenitor cells (HSPCs) play important roles in fighting systemic infection as they supply immune cells in a demand-adapted manner. Various mechanisms govern HSPC responses to infection, including cytokine signaling, niche function, and direct sensing of pathogen-derived molecules by HSPCs themselv...

    journal_title:Experimental hematology

    pub_type: 杂志文章,评审

    doi:10.1016/j.exphem.2015.11.008

    authors: Kobayashi H,Suda T,Takubo K

    更新日期:2016-02-01 00:00:00

  • An evaluation of factors affecting the in vitro bioassay for erythropoietin.

    abstract::Two main aspects of the in vitro mouse foetal liver cell assay for Erythroid Stimulating Factor (ESF) in human sera have been investigated. The haem extraction process has been shown to give specific and quantitative recovery of 59Fe labelled haem from haemoglobin thus confirming that the material assayed in human ser...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Dunn CD,Napier JA

    更新日期:1975-11-01 00:00:00

  • In vitro maintenance of hemopoietic stem cells with lymphoid and myeloid repopulating ability by a cloned murine adherent bone marrow cell line.

    abstract::Colonies containing blast cells (greater than 95%) have been characterized in 14-day cultures of normal murine bone marrow or spleen cells, stimulated by an adherent bone marrow cell line, B.Ad. These blast cell colonies occurred at a frequency of 1-2 per 2.5 X 10(4) bone marrow or 2.5 X 10(5) spleen cells and contain...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Leung LC,Johnson GR

    更新日期:1987-10-01 00:00:00

  • Cholinergic enhancement of megakaryocytopoiesis and granulocytopoiesis in culture: mediation via T-lymphocytes.

    abstract::Addition of carbamylcholine, a cholinergic analogue, to bone marrow cultures enhanced megakaryocytic and granulocytic growth by 60% and 42%, respectively. When carbamylcholine was added to spleen cells cultured in the presence of pokeweed mitogen, the resulting conditioned medium (PWM-SCM) increased the number of mega...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Chatelain C,Hamood M,De Bast M,Symann M

    更新日期:1989-12-01 00:00:00

  • Upregulated microRNA-146a expression induced by granulocyte colony-stimulating factor enhanced low-dosage chemotherapy response in aged acute myeloid leukemia patients.

    abstract::The selection of chemotherapy regimen for elderly patients with acute myeloid leukemia (AML) remains challenging. Here, we report that granulocyte colony-stimulating factor (G-CSF) upregulates the expression of microRNA (miR)-146a in a nuclear factor kappaB-dependent manner, leading to direct decreases in the expressi...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/j.exphem.2018.09.002

    authors: Li X,Xu L,Sheng X,Cai J,Liu J,Yin T,Xiao F,Chen F,Zhong H

    更新日期:2018-12-01 00:00:00

  • Thalidomide maintenance therapy maturates the T cell compartment and compromises antigen-specific antitumor immunity in patients with multiple myeloma.

    abstract::Interferon (INF)-α was the maintenance treatment of choice after autologous stem cell transplantation in multiple myeloma in the past, but currently Thalidomide is commonly used. In this prospective study, the implications of the various types of maintenance therapy on the patients T cell pattern and activation status...

    journal_title:Experimental hematology

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.exphem.2012.10.018

    authors: Herth I,Witzens-Harig M,Beckhove P,Hose D,Meissner T,Neuber B,Engelhardt M,Haas J,Neben K,Ho AD,Klein B,Goldschmidt H,Hundemer M

    更新日期:2013-03-01 00:00:00

  • Intraperitoneally injected cultured mast cells suppress recruitment and differentiation of bone marrow-derived mast cell precursors in the peritoneal cavity of W/Wv mice.

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Waki N,Kitamura Y,Kanakura Y,Asai H,Nakano T

    更新日期:1990-03-01 00:00:00

  • Preventive and therapeutic effects of gene therapy using silica nanoparticles-binding of GM-CSF gene on white blood cell production in dogs with leukopenia.

    abstract:OBJECTIVE:Our previous study has shown that granulocyte-macrophage colony-stimulating factor (GM-CSF) gene/silica nanoparticles have a leukocytosis effect in normal dogs. Therefore, this study was conducted to determine whether treatment of canine GM-CSF gene/silica nanoparticles has preventive or therapeutic effects i...

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    pub_type: 杂志文章

    doi:10.1016/j.exphem.2008.04.002

    authors: Choi EW,Koo HC,Shin IS,Chae YJ,Lee JH,Han SM,Lee SJ,Bhang DH,Park YH,Lee CW,Youn HY

    更新日期:2008-09-01 00:00:00

  • Effect of androgens on maturation and metabolism of erythroid tissue.

    abstract::Specific changes taking place in the erythroid tissue following depletion or replacement of androgens were studied in rats. The reduction of testosterone levels in blood of orchiectomized animals did occur in conjunction with a decline of erythrocyte glucose-6-phosphate (G6P) and lactate levels. No evidence of anemia ...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Molinari PF,Esber HJ,Snyder LM

    更新日期:1976-09-01 00:00:00

  • Regulation of the functional activity of mast cells and fibroblasts by mononuclear cells in murine and human chronic graft-vs.-host disease.

    abstract::Mast cell (MC)-fibroblast-immunocompetent cell interactions may play a role in the inflammatory and fibrotic processes present in chronic graft-vs.-host disease (cGVHD). Interactions between these cell types were examined in both murine and human cGVHD models. To this purpose, cell supernatants from mice or humans wit...

    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:

    authors: Levi-Schaffer F,Segal V,Barak V,Rubinchik E,Nagler A

    更新日期:1997-03-01 00:00:00

  • Differential gene expression signatures of adult peripheral blood vs cord blood monocyte-derived immature and mature dendritic cells.

    abstract:OBJECTIVE:Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos. MATERIALS AND METHODS:In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature...

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    doi:10.1016/j.exphem.2009.07.010

    authors: Jiang H,van de Ven C,Baxi L,Satwani P,Cairo MS

    更新日期:2009-10-01 00:00:00

  • Heat shock protein 70 (Hsp70) stimulates proliferation and cytolytic activity of natural killer cells.

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    journal_title:Experimental hematology

    pub_type: 杂志文章

    doi:10.1016/s0301-472x(99)00104-6

    authors: Multhoff G,Mizzen L,Winchester CC,Milner CM,Wenk S,Eissner G,Kampinga HH,Laumbacher B,Johnson J

    更新日期:1999-11-01 00:00:00

  • Kynurenine, by activating aryl hydrocarbon receptor, decreases erythropoietin and increases hepcidin production in HepG2 cells: A new mechanism for anemia of inflammation.

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    pub_type: 杂志文章

    doi:10.1016/j.exphem.2015.08.010

    authors: Eleftheriadis T,Pissas G,Antoniadi G,Liakopoulos V,Stefanidis I

    更新日期:2016-01-01 00:00:00

  • Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia.

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    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1016/j.exphem.2019.09.001

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    更新日期:2019-10-01 00:00:00