DNA replication regulated by the priming promoter.

abstract：:The Department of Energy (DOE) Joint Genome Institute (JGI) is a national user facility with massive-scale DNA sequencing and analysis capabilities dedicated to advancing genomics for bioenergy and environmental applications. Beyond generating tens of trillions of DNA bases annually, the Institute develops and maintai...

journal_title：Nucleic acids research

authors： Grigoriev IV,Nordberg H,Shabalov I,Aerts A,Cantor M,Goodstein D,Kuo A,Minovitsky S,Nikitin R,Ohm RA,Otillar R,Poliakov A,Ratnere I,Riley R,Smirnova T,Rokhsar D,Dubchak I

更新日期：2012-01-01 00:00:00

abstract：:Inhibition of an RNA processing reaction after treatment with the Ca2(+)-dependent micrococcal nuclease (MN) is often used as a criterion for the presence of a required RNA or ribonucleoprotein component in the system. Following MN digestion, the nuclease is inactivated with EGTA and radiolabeled substrate is added to...

journal_title：Nucleic acids research

authors： Wang MJ,Gegenheimer P

更新日期：1990-11-25 00:00:00

abstract：:Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host and make available such data to the community, but these databases are frequently limited in scope and/or content. BioMuta, a database of cancer-associated si...

journal_title：Nucleic acids research

authors： Dingerdissen HM,Torcivia-Rodriguez J,Hu Y,Chang TC,Mazumder R,Kahsay R

更新日期：2018-01-04 00:00:00

abstract：:Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus pola...

journal_title：Nucleic acids research

authors： Pochart P,Agoutin B,Rousset S,Chanet R,Doroszkiewicz V,Heyman T

更新日期：1993-07-25 00:00:00

abstract：:Genome-wide association (GWA) studies to map genes for complex traits are powerful yet costly. DNA-pooling strategies have the potential to dramatically reduce the cost of GWA studies. Pooling using Affymetrix arrays has been proposed and used but the efficiency of these arrays has not been quantified. We compared and...

journal_title：Nucleic acids research

authors： Macgregor S,Zhao ZZ,Henders A,Nicholas MG,Montgomery GW,Visscher PM

更新日期：2008-04-01 00:00:00

abstract：:Horizontally acquired genes are typically regulated by ancestral regulators. This regulation enables expression of horizontally acquired genes to be coordinated with that of preexisting genes. Here, we report a singular example of the opposite regulation: a horizontally acquired gene that controls an ancestral regulat...

journal_title：Nucleic acids research

authors： Choi J,Groisman EA

更新日期：2020-11-04 00:00:00

abstract：:The International Committee on Taxonomy of Viruses (ICTV) is charged with the task of developing, refining, and maintaining a universal virus taxonomy. This task encompasses the classification of virus species and higher-level taxa according to the genetic and biological properties of their members; naming virus taxa;...

journal_title：Nucleic acids research

authors： Lefkowitz EJ,Dempsey DM,Hendrickson RC,Orton RJ,Siddell SG,Smith DB

更新日期：2018-01-04 00:00:00

abstract：:Aberrant DNA methylation at CpG dinucleotides can result in epigenetic silencing of tumour suppressor genes and represents one of the earliest events in tumourigenesis. To date, however, high-throughput tools that are capable of surveying the methylation status of multiple gene promoters have been restricted to a limi...

journal_title：Nucleic acids research

authors： Mund C,Beier V,Bewerunge P,Dahms M,Lyko F,Hoheisel JD

更新日期：2005-04-28 00:00:00

abstract：:A correlation between histone acetylation and transcription has been noted for a long time, but little is known about what step(s) in the transcription cycle is influenced by acetylation. We have examined the immediate transcriptional response to histone deacetylase (HDAC) inhibition, and find that release of promoter...

journal_title：Nucleic acids research

authors： Vaid R,Wen J,Mannervik M

更新日期：2020-05-21 00:00:00

abstract：:ATRX and MeCP2 belong to an expanding group of chromatin-associated proteins implicated in human neurodevelopmental disorders, although their gene-regulatory activities are not fully resolved. Loss of ATRX prevents full repression of an imprinted gene network in the postnatal brain and in this study we address the mec...

journal_title：Nucleic acids research

authors： Kernohan KD,Vernimmen D,Gloor GB,Bérubé NG

更新日期：2014-07-01 00:00:00

abstract：:DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted r...

journal_title：Nucleic acids research

authors： Lin LS,Meyer RJ

更新日期：1987-10-26 00:00:00

abstract：:Saccharomyces cerevisiae Pif1 (ScPif1) is known as an ATP-dependent DNA helicase that plays critical roles in a number of important biological processes such as DNA replication, telomere maintenance and genome stability maintenance. Besides its DNA helicase activity, ScPif1 is also known as a single-stranded DNA (ssDN...

journal_title：Nucleic acids research

authors： Lu C,Le S,Chen J,Byrd AK,Rhodes D,Raney KD,Yan J

更新日期：2019-08-22 00:00:00

abstract：:In most bacteria, two tRNAs decode the four arginine CGN codons. One tRNA harboring a wobble inosine (tRNA(Arg)ICG) reads the CGU, CGC and CGA codons, whereas a second tRNA harboring a wobble cytidine (tRNA(Arg)CCG) reads the remaining CGG codon. The reduced genomes of Mycoplasmas and other Mollicutes lack the gene en...

journal_title：Nucleic acids research

authors： Yokobori S,Kitamura A,Grosjean H,Bessho Y

更新日期：2013-07-01 00:00:00

abstract：:Augmented biological activity in vitro has been demonstrated in oligonucleotides (oligos) modified to provide nuclease resistance, to enhance cellular uptake or to increase target affinity. How chemical modification affects the duration of effect of an oligo with potent activity has not been investigated directly. We ...

journal_title：Nucleic acids research

authors： Tam RC,Li Y,Noonberg S,Hwang DG,Lui G,Hunt CA,Garovoy MR

更新日期：1994-03-25 00:00:00

abstract：:Dlx homeobox genes are first expressed in embryonic retina at E11.5. The Dlx1/Dlx2 null retina has a reduced ganglion cell layer (GCL), with loss of late-born differentiated retinal ganglion cells (RGCs) due to increased apoptosis. TrkB signaling is proposed to regulate the dynamics of RGC apoptosis throughout develop...

journal_title：Nucleic acids research

authors： de Melo J,Zhou QP,Zhang Q,Zhang S,Fonseca M,Wigle JT,Eisenstat DD

更新日期：2008-02-01 00:00:00

abstract：:The bHLH transcription factor MyoD, the prototypical master regulator of differentiation, directs a complex program of gene expression during skeletal myogenesis. The up-regulation of the cdk inhibitor p57kip2 plays a critical role in coordinating differentiation and growth arrest during muscle development, as well as...

journal_title：Nucleic acids research

authors： Busanello A,Battistelli C,Carbone M,Mostocotto C,Maione R

更新日期：2012-09-01 00:00:00

abstract：:E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The proce...

journal_title：Nucleic acids research

authors： Dower WJ,Miller JF,Ragsdale CW

更新日期：1988-07-11 00:00:00

abstract：:WorfDB (Worm ORFeome DataBase; http://worfdb.dfci.harvard.edu) was created to integrate and disseminate the data from the cloning of complete set of approximately 19 000 predicted protein-encoding Open Reading Frames (ORFs) of Caenorhabditis elegans (also referred to as the 'worm ORFeome'). WorfDB serves as a central ...

journal_title：Nucleic acids research

authors： Vaglio P,Lamesch P,Reboul J,Rual JF,Martinez M,Hill D,Vidal M

更新日期：2003-01-01 00:00:00

abstract：:We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudopromoter. We inve...

journal_title：Nucleic acids research

authors： Ciaramella M,Sacco M,Pulitzer JF

更新日期：1988-09-26 00:00:00

abstract：:We have examined the absorption and circular dichroism spectra of intact Penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded RNA. The absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded RNA and free viral protein, ind...

journal_title：Nucleic acids research

authors： Edmondson SP,Gray DM

更新日期：1983-01-11 00:00:00

abstract：:An algorithm is presented which compares two restriction maps, yielding a measure of distance between the maps and relating the maps by an alignment. This new algorithm finds the minimum weighted sum of genetic events required to convert one map into the other, where the genetic events are the appearance/disappearance...

journal_title：Nucleic acids research

authors： Waterman MS,Smith TF,Katcher HL

更新日期：1984-01-11 00:00:00

abstract：:HIV-1 Tat protein reprograms cellular gene expression of infected as well as uninfected cells apart from its primary function of transactivating HIV-1 long terminal repeat (LTR) promoter by binding to a nascent RNA stem-loop structure known as the transactivator response region (TAR). Tat also induces chromatin remode...

journal_title：Nucleic acids research

authors： Dandekar DH,Ganesh KN,Mitra D

更新日期：2004-02-23 00:00:00

abstract：:Human Adenovirus type 5 encodes two short RNA polymerase III transcripts, the virus-associated (VA) RNAI and VA RNAII, which can adopt stable hairpin structures that resemble micro-RNA precursors. The terminal stems of the VA RNAs are processed into small RNAs (mivaRNAs) that are incorporated into RISC. It has been re...

journal_title：Nucleic acids research

authors： Xu N,Gkountela S,Saeed K,Akusjärvi G

更新日期：2009-11-01 00:00:00

abstract：:Regulation of CLB2 is important both for completion of the normal vegetative cell cycle in Saccharomyces cerevisiae and for departure from the vegetative cell cycle upon nitrogen deprivation. Cell cycle-regulated transcription of CLB2 in the G2/M phase is known to be brought about by a set of proteins including Mcm1p,...

journal_title：Nucleic acids research

authors： Van Slyke C,Grayhack EJ

更新日期：2003-08-01 00:00:00

abstract：:It is common knowledge that conserved residues evolve slowly. We challenge generality of this central tenet of molecular biology by describing the fast evolution of a conserved nucleotide position that is located in the overlap of two open reading frames (ORFs) of polyomaviruses. The de novo ORF is expressed through e...

journal_title：Nucleic acids research

authors： Lauber C,Kazem S,Kravchenko AA,Feltkamp MC,Gorbalenya AE

更新日期：2015-05-26 00:00:00

abstract：:Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm3(2,2,7)G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid an...

journal_title：Nucleic acids research

authors： Iwase R,Sekine M,Tokumoto Y,Ohshima Y,Hata T

更新日期：1989-11-25 00:00:00

abstract：:The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, ...

journal_title：Nucleic acids research

authors： Pestov DG,Dayn A,Siyanova EYu,George DL,Mirkin SM

更新日期：1991-12-11 00:00:00

abstract：:A coiled-coil microtubule-bundling protein, p180, was originally identified as one of the ribosome receptor candidates on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported that p180 plays crucial roles in upregulating collagen biosynthesis, mainly by facilitating ...

journal_title：Nucleic acids research

authors： Ueno T,Kaneko K,Sata T,Hattori S,Ogawa-Goto K

更新日期：2012-04-01 00:00:00

abstract：:We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchange chromatography. ...

journal_title：Nucleic acids research

authors： Blommaert FA,Saris CP

更新日期：1995-04-25 00:00:00

abstract：:Remodelling protein nucleic acid interfaces is an important biological task, which is often carried out by nucleic acid stimulated ATPases of the Swi2/Snf2 superfamily. Here we study the mechano-chemical cycle of such an ATPase, namely the catalytic domain of the Sulfolobus solfataricus Rad54 homologue (SsoRad54cd), b...

journal_title：Nucleic acids research

authors： Lewis R,Dürr H,Hopfner KP,Michaelis J

更新日期：2008-04-01 00:00:00