The purification, structure and synthesis of proticine 3.

abstract：:Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequenci...

journal_title：Journal of medical microbiology

authors： Fasihi Y,Fooladi S,Mohammadi MA,Emaneini M,Kalantar-Neyestanaki D

更新日期：2017-09-01 00:00:00

abstract：:The sensitivity of Escherichia coli strains to 80% normal human serum (NHS) and the relative importance of the classical and alternative complement pathways was assessed in relation to K1, K5, and O antigen carriage. Strains of each of the common O-serogroups, O1, O2, O4, O6, O7, O9, O18 and O75, smooth strains not ty...

journal_title：Journal of medical microbiology

authors： Devine DA,Roberts AP

更新日期：1994-08-01 00:00:00

abstract：:Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...

journal_title：Journal of medical microbiology

authors： Riley LK,Caffrey CJ,Musille VS,Meyer JK

更新日期：1992-07-01 00:00:00

abstract：:Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

journal_title：Journal of medical microbiology

authors： Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

更新日期：2002-05-01 00:00:00

abstract：:A cosmid DNA library had been constructed previously from 40-kb fragments of genomic DNA from a virulent invasive strain of Salmonella enterica serotype Typhimurium (TML) in an avirulent hypo-invasive Typhimurium strain (LT7). Selection of invasive clones from the library was attempted by iterative passage through a r...

journal_title：Journal of medical microbiology

authors： Robey M,Morgan E,Lodge JM,Bolton AJ,Martin GD,Brown NL,Stephen J

更新日期：2002-03-01 00:00:00

abstract：:Encapsulation of purified tuberculoproteins in liposomes augmented their ability to elicit delayed hypersensitivity reactions in BCG-immune rats. The effect was most marked with a low-molecular-weight tuberculopeptide that was relatively poor at eliciting reactions when in free form. These findings indicate that, in a...

journal_title：Journal of medical microbiology

authors： Pimm MV,Baldwin RW

更新日期：1984-12-01 00:00:00

abstract：:Tests with 140 strains representing Escherichia coli, Klebsiella aerogenes, K. ozaenae, Proteus mirabilis, P. vulgaris, P. rettgeri and P. morganii in a defined medium supplemented with 0-09M dimethylamine (DMA) and 0-1M potassium nitrate showed that at least 89% of the 136 strains able to reduce nitrates produced up ...

journal_title：Journal of medical microbiology

authors： Coloe PJ,Hayward NJ

更新日期：1976-05-01 00:00:00

abstract：:The Pro-Pro-Glu (PPE) protein family of Mycobacterium tuberculosis includes 69 glycine-rich proteins with a conserved N-terminal domain. Their role in tuberculosis is unknown, but it has been speculated that they may have an important immunological significance. In this investigation, the immunogenicity of the ppe44 (...

journal_title：Journal of medical microbiology

authors： Bonanni D,Rindi L,Lari N,Garzelli C

更新日期：2005-05-01 00:00:00

abstract：:Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...

journal_title：Journal of medical microbiology

authors： Samaranayake YH,Samaranayake LP

更新日期：1994-11-01 00:00:00

abstract：:A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test...

journal_title：Journal of medical microbiology

authors： Shimaoka M,Yoh M,Takarada Y,Yamamoto K,Honda T

更新日期：1996-03-01 00:00:00

abstract：:Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

journal_title：Journal of medical microbiology

authors： Paciorek J

更新日期：2002-07-01 00:00:00

abstract：:Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...

journal_title：Journal of medical microbiology

authors： Zöllner B,Wuthe HH

更新日期：1993-01-01 00:00:00

abstract：:The closely related Clostridium novyi and Clostridium botulinum types C and D are of current interest because of their association with serious infections in injecting drug users (C. novyi type A) and equine and feline dysautonomias (C. botulinum types C/D). The species are defined by the major toxins they produce: th...

journal_title：Journal of medical microbiology

authors： Heffron A,Poxton IR

更新日期：2007-02-01 00:00:00

abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...

journal_title：Journal of medical microbiology

authors： Descheemaeker P,VAN Loock F,Hauchecorne M,Vandamme P,Goossens H

更新日期：2000-05-01 00:00:00

abstract：:Alpha protoxin of Staphylococcus aureus "Wood 46" was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinit...

journal_title：Journal of medical microbiology

authors： Wiseman GM,Caird JD,Fackrell HB

更新日期：1975-02-01 00:00:00

abstract：:Clostridium botulinum type A toxin is the most prevalent cause of naturally occurring outbreaks of human botulism in the world. The active dichain neurotoxin molecule is composed of a heavy chain (H-chain) of ~100 kDa with the carboxy-terminal end consisting of a receptor-binding (HC) domain, while the amino-terminal ...

journal_title：Journal of medical microbiology

authors： Jones RGA,Marks JD

更新日期：2013-06-01 00:00:00

abstract：:Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...

journal_title：Journal of medical microbiology

authors： Hoenigl M,Grisold AJ,Valentin T,Leitner E,Zarfel G,Renner H,Krause R

更新日期：2010-04-01 00:00:00

abstract：:The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK,Tarpey PA

更新日期：1999-02-01 00:00:00

abstract：:This report focuses on the molecular characterization of 22 extended-spectrum beta-lactamase-producing Escherichia coli isolates collected in a German university hospital during a period of 9 months in 2006. Relationship analysis of clinical isolates was done via PFGE, multilocus sequence typing, plasmid profiling and...

journal_title：Journal of medical microbiology

authors： Cullik A,Pfeifer Y,Prager R,von Baum H,Witte W

更新日期：2010-05-01 00:00:00

abstract：:A total of 817 human clinical isolates of Clostridium difficile from all Australian states were screened for A(-)B(+) strains by toxin gene PCR assays. Nine (1.1 %) strains were confirmed to be A(-)B(+) by enzyme immunoassay for toxin production. Of these, six (66.7 %) were binary toxin-positive by PCR. Using PCR ribo...

journal_title：Journal of medical microbiology

authors： Elliott B,Squire MM,Thean S,Chang BJ,Brazier JS,Rupnik M,Riley TV

更新日期：2011-08-01 00:00:00

abstract：:Serology remains the mainstay for diagnosis of Epstein-Barr virus (EBV) infection. This study compared two automated platforms (BioPlex 2200 and Architect i2000SR) to test three EBV serological markers: viral capsid antigen (VCA) immunoglobulins of class M (IgM), VCA immunoglobulins of class G (IgG) and EBV nuclear an...

journal_title：Journal of medical microbiology

authors： Al Sidairi H,Binkhamis K,Jackson C,Roberts C,Heinstein C,MacDonald J,Needle R,Hatchette TF,LeBlanc JJ

更新日期：2017-11-01 00:00:00

abstract：:This is the first report, to our knowledge, of two temporally and geographically related nosocomial Asaia lannensis infections in a paediatric setting. Two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. The physical location of both pa...

journal_title：Journal of medical microbiology

authors： Juretschko S,Beavers-May TK,Stovall SH

更新日期：2010-07-01 00:00:00

abstract：:Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant ...

journal_title：Journal of medical microbiology

authors： Brown T

更新日期：1979-08-01 00:00:00

abstract：:Diagnosis of human immunodeficiency virus (HIV) infection by antibody-based testing allows for some recently infected individuals to be falsely assessed as non-infected. Since such individuals often have high viral loads and are capable of transmitting HIV, it is an imperative public health need to identify these indi...

journal_title：Journal of medical microbiology

authors： Ren A,Louie B,Rauch L,Castro L,Liska S,Klausner JD,Pandori MW

更新日期：2008-10-01 00:00:00

abstract：:The care of many patients undergoing long-term bladder catheterization is frequently complicated by infection with Proteus mirabilis. These organisms colonize the catheter, forming surface biofilm communities, and their urease activity generates alkaline conditions under which crystals of magnesium ammonium phosphate ...

journal_title：Journal of medical microbiology

authors： Jones BV,Mahenthiralingam E,Sabbuba NA,Stickler DJ

更新日期：2005-09-01 00:00:00

abstract：:A total of 118 meticillin-resistant Staphylococcus aureus (MRSA) and 140 meticillin-susceptible S. aureus (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylo...

journal_title：Journal of medical microbiology

authors： Hu DL,Omoe K,Inoue F,Kasai T,Yasujima M,Shinagawa K,Nakane A

更新日期：2008-09-01 00:00:00

abstract：:During the first quarter of 1996, a major outbreak of clinical infection caused by multiresistant Klebsiella pneumoniae (MRKP) occurred in the neonatal ward of the 'Maternité Wassila Bourguiba' in Tunis, Tunisia. In total, 32 isolates of MRKP, comprising 23 clinical isolates and nine surveillance isolates, were recove...

journal_title：Journal of medical microbiology

authors： Ben-Hamouda T,Foulon T,Ben-Cheikh-Masmoudi A,Fendri C,Belhadj O,Ben-Mahrez K

更新日期：2003-05-01 00:00:00

abstract：:Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypica...

journal_title：Journal of medical microbiology

authors： Collins T,Levett PN

更新日期：1989-11-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread of some MRSA epidemic clones is well documented. In Brazil, and more recently in Portugal, a considerable number of hospital infections has been caused by a unique multiresistant MRSA clone design...

journal_title：Journal of medical microbiology

authors： Coimbra MVDS,Teixeira LA,Ramos RLB,Predari SC,Castello L,Famiglietti A,Vay C,Klan L,Figueiredo AMS

更新日期：2000-02-01 00:00:00

abstract：:Multi-drug-resistant tuberculosis (MDR-TB) is a major public-health problem, because treatment is complicated and patients remain infectious for months or years, despite receiving the best available therapy. To gain better understanding of MDR-TB, a retrospective study was initiated to determine the level of drug resi...

journal_title：Journal of medical microbiology

authors： Dam T,Isa M,Bose M

更新日期：2005-03-01 00:00:00