Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b.

abstract：:We studied intracellular pH (pHi) regulation in the absence of HCO3- in single mesangial cells (MCs) with the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(and -6)carboxyfluorescein. Our approach was to acid load the cells by an NH+4 prepulse and to monitor the subsequent pHi recovery. Previous work on MCs and other ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyarsky G,Ganz MB,Cragoe EJ Jr,Boron WF

更新日期：1990-08-01 00:00:00

abstract：:In this study, we describe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievirus B6 replication in living Buffalo green monkey kidney (BGMK) cells via Tat peptide delivery. A nuclease-resistant MB containing 2'-O-methyl RNA bases with phosphorothioate internucleotide linka...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh HY,Yates MV,Mulchandani A,Chen W

更新日期：2008-11-11 00:00:00

abstract：:Studies on the structure-function relationship of diphtheria toxin are reported. New methods are described for the preparation of pure intact ("unnicked") toxin and for the preparation of the individual A and B chains. A biological assay method for the B chain is also presented, as well as a method for the labelling o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Everse J,Lappi DA,Beglau JM,Lee CL,Kaplan NO

更新日期：1977-02-01 00:00:00

abstract：:We are designing simple peptide ion channels as model systems for the study of the physical principles controlling conduction through ion-channel proteins. Here we report on an uncharged peptide, Ac-(Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2, designed to form an aggregate of parallel, amphiphilic, membrane-spanning alpha-he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kienker PK,DeGrado WF,Lear JD

更新日期：1994-05-24 00:00:00

abstract：:The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1. Transcription was detected with DNA representing IE region 2 when the IE r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomsen DR,Stenberg RM,Goins WF,Stinski MF

更新日期：1984-02-01 00:00:00

abstract：:In skeletal muscle, transcription of the gene encoding the mouse type Ialpha (RIalpha) subunit of the cAMP-dependent protein kinase is initiated from the alternative noncoding first exons 1a and 1b. Here, we report that activity of the promoter upstream of exon 1a (Pa) depends on two adjacent E boxes (E1 and E2) in NI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barradeau S,Imaizumi-Scherrer T,Weiss MC,Faust DM

更新日期：2001-04-24 00:00:00

abstract：:Langerhans cells are a subset of dendritic cells (DCs) found in the human epidermis with unique morphological and molecular properties that enable their function as "sentinels" of the immune system. DCs are pivotal in the initiation and regulation of primary MHC class I restricted T lymphocyte immune responses and are...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacAry PA,Lindsay M,Scott MA,Craig JI,Luzio JP,Lehner PJ

更新日期：2001-03-27 00:00:00

abstract：:The effect of x-rays on the rate of semiconservative DNA replication was investigated by autoradiography in single cells obtained from normal individuals and from patients having ataxia-telangiectasia (AT). In the five AT cell strains studied, the rate of DNA synthesis was inhibited to a lesser extent that in two norm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaspers NG,Bootsma D

更新日期：1982-04-01 00:00:00

abstract：:Egg activation is the process in which mature oocytes are released from developmental arrest and gain competency for embryonic development. In Drosophila and other arthropods, eggs are activated by mechanical pressure in the female reproductive tract, whereas in most other species, eggs are activated by fertilization....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Q,Wolfner MF

更新日期：2019-09-17 00:00:00

abstract：:Intense proteolysis of cytoskeletal proteins occurs in brain within minutes of transient ischemia, possibly because of the activation of calcium-sensitive proteases (calpains). This proteolytic event precedes overt signs of neuronal degeneration, is most pronounced in regions of selective neuronal vulnerability, and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee KS,Frank S,Vanderklish P,Arai A,Lynch G

更新日期：1991-08-15 00:00:00

abstract：:Recent cryoEM density maps of Abeta(42) fibrils obtained at low pH revealed two protofilaments winding around a hollow core raising the question if such tubular structures also exist at physiological pH. Based on the cryoEM measurements and on NMR data, we probe amyloid fibril organizations corresponding to the observ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller Y,Ma B,Tsai CJ,Nussinov R

更新日期：2010-08-10 00:00:00

abstract：:We studied the abundance and structure of globin mRNAs present in K562 cells both before and after induction of hemoglobin synthesis by hemin. In vitro translation of poly(A)+ RNA from K562 cells generated protein products corresponding to alpha, A gamma-, G gamma-, epsilon-, and zeta-globin mRNAs. Individual globin m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dean A,Ley TJ,Humphries RK,Fordis M,Schechter AN

更新日期：1983-09-01 00:00:00

abstract：:Bone marrow mononuclear cells (BMC) from patients with ischemic cardiomyopathy (ICMP) show a reduced neovascularization capacity in vivo. NO plays an important role in neovascularization, and NO bioavailability is typically reduced in patients with ICMP. We investigated whether the impaired neovascularization capacity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasaki K,Heeschen C,Aicher A,Ziebart T,Honold J,Urbich C,Rossig L,Koehl U,Koyanagi M,Mohamed A,Brandes RP,Martin H,Zeiher AM,Dimmeler S

更新日期：2006-09-26 00:00:00

abstract：:Disruption of neurotransmitter vesicle dynamics (transport, capacity, release) has been implicated in a variety of neurodegenerative and neuropsychiatric conditions. Here, we report a novel mouse model of enhanced vesicular function via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohr KM,Bernstein AI,Stout KA,Dunn AR,Lazo CR,Alter SP,Wang M,Li Y,Fan X,Hess EJ,Yi H,Vecchio LM,Goldstein DS,Guillot TS,Salahpour A,Miller GW

更新日期：2014-07-08 00:00:00

abstract：:Many cells exhibit periodic transient increases in cytosolic calcium levels rather than a sustained rise when stimulated by a hormone or growth factor. We propose here a molecular model that accounts for periodic calcium spiking induced by a constant stimulus. Four elements give rise to repetitive calcium transients: ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer T,Stryer L

更新日期：1988-07-01 00:00:00

abstract：:Molecular adaptation is typically assumed to proceed by sequential fixation of beneficial mutations. In diploids, this picture presupposes that for most adaptive mutations, the homozygotes have a higher fitness than the heterozygotes. Here, we show that contrary to this expectation, a substantial proportion of adaptiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sellis D,Callahan BJ,Petrov DA,Messer PW

更新日期：2011-12-20 00:00:00

abstract：:Recombination is unknown in natural populations of Rivulus marmoratus, a selfing hermaphrodite, and genetic variation is likely due to mutation alone. DNA fingerprinting with an array of microsatellite [e.g., (CT)9] and minisatellite (e.g., the 33.15 core sequence) probes reveals very high clonal diversity within samp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turner BJ,Elder JF Jr,Laughlin TF,Davis WP,Taylor DS

更新日期：1992-11-15 00:00:00

abstract：:Oxidation of molecular hydrogen catalyzed by [NiFe] hydrogenases is a widespread mechanism of energy generation among prokaryotes. Biosynthesis of the H2-oxidizing enzymes is a complex process subject to positive control by H2 and negative control by organic energy sources. In this report we describe a novel signal tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lenz O,Friedrich B

更新日期：1998-10-13 00:00:00

abstract：:Ab initio multi-reference second-order perturbation theory computations are used to explore the photochemical behavior of two ion pairs constituted by a chloride counterion interacting with either a rhodopsin or bacteriorhodopsin chromophore model (i.e., the 4-cis-gamma-methylnona-2,4,6,8-tetraeniminium and all-trans-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cembran A,Bernardi F,Olivucci M,Garavelli M

更新日期：2005-05-03 00:00:00

abstract：:Complex II inhibitors 3-nitropropionic acid (3NP) and malonate cause striatal damage reminiscent of Huntington's disease and have been shown to involve oxidative stress in their pathogenesis. Because nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent transcriptional activation by means of the antioxidant res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calkins MJ,Jakel RJ,Johnson DA,Chan K,Kan YW,Johnson JA

更新日期：2005-01-04 00:00:00

abstract：:Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning JE,Wolstenholme DR,Ryan RS,Hunter JA,Richards OC

更新日期：1971-06-01 00:00:00

abstract：:Ultraviolet irradiation of the [3H]colchicine-tubulin complex leads to direct photolabeling of tubulin with low but practicable efficiency. The bulk (70% to greater than 90%) of the labeling occurs on beta-tubulin and appears early after irradiation, whereas alpha-tubulin is labeled later. The labeling ratio of beta-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolff J,Knipling L,Cahnmann HJ,Palumbo G

更新日期：1991-04-01 00:00:00

abstract：:The tails of newborn rats, consisting of slender continuous cones of avascular cartilage, were transplanted in subcutaneous spaces of allogeneic adolescents; colloidal carbon was injected in a vein before the transplants were harvested on days 7-28. Between 97 and 100% of the transplants were accepted, underwent diffe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huggins CB,Anderson KP

更新日期：1976-09-01 00:00:00

abstract：:N-type Ca2+ channels mediate Ca2+ influx, which initiates fast exocytosis of neurotransmitters at synapses, and they interact directly with the SNARE proteins syntaxin and SNAP-25 (synaptosome-associated protein of 25 kDa) through a synaptic protein interaction (synprint) site in the intracellular loop connecting doma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheng ZH,Yokoyama CT,Catterall WA

更新日期：1997-05-13 00:00:00

abstract：:Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bystricky K,Heun P,Gehlen L,Langowski J,Gasser SM

更新日期：2004-11-23 00:00:00

abstract：:IFN-alpha/beta plays an essential role in innate immunity against viral and bacterial infection. Among the proteins induced by IFN-alpha/beta are the ubiquitin-like ISG15 protein and its E1- (Ube1L) and E2- (UbcH8) conjugating enzymes, leading to the conjugation of ISG15 to cellular proteins. It is likely that ISG15 c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao C,Denison C,Huibregtse JM,Gygi S,Krug RM

更新日期：2005-07-19 00:00:00

abstract：:Site-specific recombination is responsible for a broad range of biological phenomena, including DNA inversion, resolution of transposition intermediates, and the integration and excision of bacteriophage genomes. Integration of mycobacteriophage L5 is catalyzed by a phage-encoded integrase with recombination occurring...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peña CE,Kahlenberg JM,Hatfull GF

更新日期：2000-07-05 00:00:00

abstract：:Mechanisms of estrogen-induced tumorigenesis in the target organ are not well understood. It has been suggested that oxidative stress resulting from metabolic activation of carcinogenic estrogens plays a critical role in estrogen-induced carcinogenesis. We tested this hypothesis by using an estrogen-induced hamster re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat HK,Calaf G,Hei TK,Loya T,Vadgama JV

更新日期：2003-04-01 00:00:00

abstract：:In many adult tissues, mesenchymal stem cells (MSCs) are closely associated with perivascular niches and coexpress many markers in common with pericytes. The ability of pericytes to act as MSCs, however, remains controversial. By using genetic lineage tracing, we show that some pericytes differentiate into specialized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feng J,Mantesso A,De Bari C,Nishiyama A,Sharpe PT

更新日期：2011-04-19 00:00:00

abstract：:Hypertonicity-induced binding of the transcription factor TonEBP/OREBP to its cognate DNA element, ORE/TonE, is associated with increased transcription of several osmotically regulated genes. Previously, it was found that hypertonicity rapidly causes nuclear translocation and phosphorylation of TonEBP/OREBP and, more ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferraris JD,Williams CK,Persaud P,Zhang Z,Chen Y,Burg MB

更新日期：2002-01-22 00:00:00