Abstract:
:In GH3 pituitary cell homogenates, acetylcholine (ACh) (IC50 200 nM) inhibits adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in a concentration- and GTP-dependent manner. Maximal inhibition was obtained with 10 microM ACh and corresponded to approximately a 50% decrease in basal enzyme activity. ACh inhibition is antagonized by atropine and is mimicked by muscarinic receptor agonists, but not by nicotine. ACh reduces the adenylate cyclase stimulation by vasoactive intestinal peptide (VIP), without changing its EC50. In intact GH3 cells, ACh decreases the cyclic AMP content and the rate of prolactin release in a concentration-dependent manner. When the cells are simultaneously exposed to VIP and ACh, the VIP-induced increases in cyclic AMP accumulation and prolactin release are reduced by 80% and 40%, respectively. The potency of VIP is not significantly changed by the presence of ACh, and vice versa.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Onali P,Eva C,Olianas MC,Schwartz JP,Costa Esubject
Has Abstractpub_date
1983-09-01 00:00:00pages
189-94issue
2eissn
0026-895Xissn
1521-0111journal_volume
24pub_type
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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更新日期:1987-07-01 00:00:00
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journal_title:Molecular pharmacology
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更新日期:1993-10-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:1992-10-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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