DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.

Abstract:

:The biochemical and morphological differentiation of actinomycetes makes them academically and economically interesting. Their secondary metabolites provide the majority of medically and agriculturally important antibiotics (streptomycete genes may also be the primary source of clinically important antibiotic resistance); their complex morphological developmental cycle involves a series of changes from vegetative mycelial growth to spore formation. Recombinant DNA technology would add a powerful new dimension to the analysis of these various aspects of actinomycete biology and would also facilitate the development of industrial strains with increased antibiotic yield, or capable of making new antibiotics. For most of these purposes, cloning of genes within and between actinomycetes is required to study the expression of particular genes in genetic backgrounds defined by mutations of the characters under study. To achieve this, we have now developed a method for molecular cloning involving the transfer of genes between unrelated streptomycetes.

journal_name

Nature

journal_title

Nature

authors

Thompson CJ,Ward JM,Hopwood DA

doi

10.1038/286525a0

subject

Has Abstract

pub_date

1980-07-31 00:00:00

pages

525-7

issue

5772

eissn

0028-0836

issn

1476-4687

journal_volume

286

pub_type

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