Mitochondrial release of Ca2+ during sustained nerve activity in the electric organ of Torpedo marmorata.

Abstract:

:The calcium content of mitochondria isolated from the Torpedo electric organ was determined by atomic absorption spectroscopy. The mitochondrial fraction was found to contain 830 ng-atom Ca/mg protein. For comparison a fraction of isolated nerve endings contained 250 ng-atom Ca/mg protein. When tissue was continuously stimulated at a frequency of 5 Hz via the electromotor nerves, the Ca content of the mitochondrial fraction which is likely to contain both pre- and postsynaptic mitochondria was reduced proportionally to the decrease in transmitter content of the isolated nerve terminal fraction. After 5,000 pulses, the mitochondrial fraction had lost 80% of its Ca contents. When tissue was stimulated after previous perfusion with d-tubocurarine and blockade of electrical response the same number of pulses caused only a loss of 20--40% in the Ca content of the mitochondrial fraction. Release of transmitter was not affected by d-tubocurarine. The results suggest that repetitive stimulation in the absence of postsynaptic receptor blockade causes loss of Ca from both nerve terminal mitochondria and mitochondria of the myotube-derived electrocytes. In the presence of d-tubocurarine Ca is lost from presynaptic mitochondria only.

journal_name

Exp Brain Res

authors

Schmidt R,Zimmermann H

doi

10.1007/BF00237520

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

405-17

issue

4

eissn

0014-4819

issn

1432-1106

journal_volume

38

pub_type

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