The use of procion dyes for light microscopy of the frog lens.

Abstract:

:Protocols for light microscopy of frog lenses that result in good visibility of cell structure from the lens surface to its nucleus are presented. The lenses are fixed in 10% neutral formalin in 0.06 M phosphate buffer and are embedded in Epon 812. Following staining of the thick sections with selected Procion dyes, essentially every cell in the section plane of the lens can be visualized by simple light microscopy without fluorescence. The methods and dyes allow measurement of cell dimensions at all depths in the lens and allow investigation of cell packing geometry. The techniques should be generally useful for studying normal lens structure and the alteration of structure induced by cataractogenesis.

authors

Rae JL,Truitt KD,Kuszak JR

subject

Has Abstract

pub_date

1983-09-01 00:00:00

pages

1167-71

issue

9

eissn

0146-0404

issn

1552-5783

journal_volume

24

pub_type

杂志文章
  • Effects of a fixation target on torsional optokinetic nystagmus.

    abstract:PURPOSE:To investigate the effects of an imaginary and a visual target on torsional optokinetic nystagmus (tOKN) and directional symmetry of tOKN. METHODS:Torsional OKN was induced by a rotating random dot pattern (52 degrees in diameter, constant angular velocity: +/-30 deg/sec to +/-52 deg/sec) with an imaginary or ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Suzuki Y,Shinmei Y,Nara H,Ifukube T

    更新日期:2000-09-01 00:00:00

  • Nitric oxide as a mediator of parasympathetic vasodilation in ocular and extraocular tissues in the rabbit.

    abstract:PURPOSE:The aim of this study was to investigate in rabbits the relationship between nitric oxide and the noncholinergic vasodilation caused by facial nerve stimulation in the eye and some extraocular tissues. METHODS:Uveal vascular resistance was determined by measuring simultaneously the flow from a cannulated vorte...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Nilsson SF

    更新日期:1996-09-01 00:00:00

  • Platelets adhering to the vascular wall mediate postischemic leukocyte-endothelial cell interactions in retinal microcirculation.

    abstract:PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed region...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-0526

    authors: Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

    更新日期:2004-03-01 00:00:00

  • Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.

    abstract:PURPOSE:To characterize the expression patterns of myosin heavy chain (MyHC) isoforms in mouse extraocular muscles (EOMs) during postnatal development. METHODS:MyHC isoform expression in mouse EOMs from postnatal day (P)0 to 3 months was evaluated by quantitative polymerase chair reaction (qPCR) and immunohistochemist...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5937

    authors: Zhou Y,Liu D,Kaminski HJ

    更新日期:2010-12-01 00:00:00

  • Cone differentiation with no photopigment coexpression.

    abstract:PURPOSE:To decide whether the transitory coexpression of cone visual pigments described in the developing rat and gerbil retina is a universal feature of dichromatic mammalian species. METHODS:The rabbit, a species widely used in eye research, was selected for the study and a search made for the presence of cones that...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Szepessy Z,Lukáts A,Fekete T,Barsi A,Röhlich P,Szél A

    更新日期:2000-09-01 00:00:00

  • Myopic refractive error in adult Latinos: the Los Angeles Latino Eye Study.

    abstract:PURPOSE:To estimate gender- and age-specific prevalence of myopia and identify risk indicators for myopia in a population-based sample of Latino adults aged 40 years and older in La Puente, California. METHODS:Noncycloplegic automated refraction with supplemental standardized subjective refraction was performed for pr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-1153

    authors: Tarczy-Hornoch K,Ying-Lai M,Varma R,Los Angeles Latino Eye Study Group.

    更新日期:2006-05-01 00:00:00

  • The effect of ouabain on volume regulation in the rat lens.

    abstract::In an anisotonic medium the tendency of the lens to swell or shrink in response to the osmotic gradient is countered by a change in the content of lens solute, so that the change in the volume of lens water is minimal. This phenomenon is known as volume regulation. The rapid phase of volume regulation occurs in less t...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Patterson JW

    更新日期:1981-01-01 00:00:00

  • Properties of perimetric threshold estimates from full threshold, ZEST, and SITA-like strategies, as determined by computer simulation.

    abstract:PURPOSE:To investigate the accuracy and precision of threshold estimates returned by two Bayesian perimetric strategies, staircase-QUEST or SQ (a Swedish interactive threshold algorithm [SITA]-like strategy) and ZEST (zippy estimation by sequential testing), and to compare these measures with those of the full-threshol...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-0023

    authors: Turpin A,McKendrick AM,Johnson CA,Vingrys AJ

    更新日期:2003-11-01 00:00:00

  • Implantation and explantation of a wireless epiretinal retina implant device: observations during the EPIRET3 prospective clinical trial.

    abstract:PURPOSE:Visual sensations in patients with blindness and retinal degenerations may be restored by electrical stimulation of retinal neurons with implantable microelectrode arrays. A prospective trial was initiated to evaluate the safety and efficacy of a wireless intraocular retinal implant (EPIRET3) in six volunteers ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 临床试验,杂志文章

    doi:10.1167/iovs.08-2752

    authors: Roessler G,Laube T,Brockmann C,Kirschkamp T,Mazinani B,Goertz M,Koch C,Krisch I,Sellhaus B,Trieu HK,Weis J,Bornfeld N,Röthgen H,Messner A,Mokwa W,Walter P

    更新日期:2009-06-01 00:00:00

  • Underestimate of tonometric readings after photorefractive keratectomy increases at higher intraocular pressure levels.

    abstract:PURPOSE:To determine whether tonometric readings of increases in intraocular pressure (IOP) during the water-drinking test (WDT) are affected by variations in central corneal thickness (CCT) induced by photorefractive keratectomy (PRK). METHODS:Data from 30 randomly selected eyes of 30 patients (18 men and 12 women; m...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-1240

    authors: Tamburrelli C,Giudiceandrea A,Vaiano AS,Caputo CG,Gullà F,Salgarello T

    更新日期:2005-09-01 00:00:00

  • In Vivo Measurements of Prelamina and Lamina Cribrosa Biomechanical Properties in Humans.

    abstract:Purpose:To develop and use a custom virtual fields method (VFM) to assess the biomechanical properties of human prelamina and lamina cribrosa (LC) in vivo. Methods:Clinical data of 20 healthy, 20 ocular hypertensive (OHT), 20 primary open-angle glaucoma, and 16 primary angle-closure glaucoma eyes were analyzed. For ea...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.61.3.27

    authors: Zhang L,Beotra MR,Baskaran M,Tun TA,Wang X,Perera SA,Strouthidis NG,Aung T,Boote C,Girard MJA

    更新日期:2020-03-09 00:00:00

  • Optical coherence tomographic correlates of angiographic subtypes of occult choroidal neovascularization.

    abstract:PURPOSE:To compare the optical coherence tomographic (OCT) correlates of two previously described angiographic subtypes of occult choroidal neovascularization (CNV). METHODS:We retrospectively analyzed 17 consecutive patients with previously untreated occult CNV who underwent both fluorescein angiography (FA) and volu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验

    doi:10.1167/iovs.13-12302

    authors: Hariri A,Heussen FM,Nittala MG,Sadda SR

    更新日期:2013-12-09 00:00:00

  • Spasmus nutans. A long-term follow-up.

    abstract:PURPOSE:Nystagmus, head nodding, and anomalous head position are symptoms of spasmus nutans. This disorder appears in early childhood and is thought to be self-limited. However, the visual outcome of patients with spasmus nutans is unclear. The resolution of nystagmus has not been proven with quantitative eye movement ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Gottlob I,Wizov SS,Reinecke RD

    更新日期:1995-12-01 00:00:00

  • Imaging Laser-Induced Choroidal Neovascularization in the Rodent Retina Using Optical Coherence Tomography Angiography.

    abstract:PURPOSE:The purpose of this study was to evaluate the performance of optical coherence tomography angiography (OCTA) in visualizing laser-induced choroidal neovascularization (CNV) in the rodent retina. METHODS:Choroidal neovascularization was induced via laser photocoagulation in 2 male Brown Norway rats and 2 male C...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-18946

    authors: Park JR,Choi W,Hong HK,Kim Y,Jun Park S,Hwang Y,Kim P,Joon Woo S,Hyung Park K,Oh WY

    更新日期:2016-07-01 00:00:00

  • Intravitreal Stanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization.

    abstract:Purpose:The purpose of this study was to investigate the impact of stanniocalcin-1 (STC-1), a photoreceptor-protective glycoprotein, on the development of choroidal neovascularization (CNV) in relation to VEGF and its main receptor (VEGFR2) expression after laser injury. Methods:In rats, CNV was induced by laser photo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-23083

    authors: Zhao M,Xie W,Tsai SH,Hein TW,Rocke BA,Kuo L,Rosa RH Jr

    更新日期:2018-02-01 00:00:00

  • Kininogen in autoimmune uveitis: decrease in peripheral blood stream versus increase in target tissue.

    abstract:PURPOSE:Equine recurrent uveitis (ERU) is an incurable disease affecting the inner eye that leads to blindness, through activated T cells that pass the blood-retinal barrier and destroy the retina. Serum markers are a desirable choice for monitoring development of disease, as serum is easy accessible and the markers co...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4094

    authors: Zipplies JK,Hauck SM,Schoeffmann S,Amann B,van der Meijden CH,Stangassinger M,Ueffing M,Deeg CA

    更新日期:2010-01-01 00:00:00

  • The novel Rho kinase (ROCK) inhibitor K-115: a new candidate drug for neuroprotective treatment in glaucoma.

    abstract:PURPOSE:To investigate the effect of K-115, a novel Rho kinase (ROCK) inhibitor, on retinal ganglion cell (RGC) survival in an optic nerve crush (NC) model. Additionally, to determine the details of the mechanism of K-115's neuroprotective effect in vivo and in vitro. METHODS:ROCK inhibitors, including K-115 and fasud...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13842

    authors: Yamamoto K,Maruyama K,Himori N,Omodaka K,Yokoyama Y,Shiga Y,Morin R,Nakazawa T

    更新日期:2014-10-02 00:00:00

  • Quantitative retinal protein analysis after optic nerve transection reveals a neuroprotective role for hepatoma-derived growth factor on injured retinal ganglion cells.

    abstract:PURPOSE:Retinal ganglion cell (RGC) degeneration is an important cause of visual impairment and can be modeled by optic nerve transection, which causes the death of 90% of RGCs within 14 days postaxotomy. We performed a proteomic study to identify and quantify proteins in the rat retina after optic nerve transection. O...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8350

    authors: Hollander A,D'Onofrio PM,Magharious MM,Lysko MD,Koeberle PD

    更新日期:2012-06-26 00:00:00

  • Impact of cell source on human cornea reconstructed by tissue engineering.

    abstract:PURPOSE:To investigate the effect of the tissue origin of stromal fibroblasts and epithelial cells on reconstructed corneas in vitro. METHODS:Four types of constructs were produced by the self-assembly approach using the following combinations of human cells: corneal fibroblasts/corneal epithelial cells, corneal fibro...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2001

    authors: Carrier P,Deschambeault A,Audet C,Talbot M,Gauvin R,Giasson CJ,Auger FA,Guérin SL,Germain L

    更新日期:2009-06-01 00:00:00

  • A fluorescence-quenching assay for measuring permeability of reconstituted lens MIP26.

    abstract::A sensitive fluorometric assay for measuring permeability of liposomes containing bovine lens MIP26 has been developed and characterized. Lens membrane proteins were isolated and incorporated into artificial membranes composed of bovine brain phospholipids, sphingomyelin and the fluorescent probe, N-4-nitrobenzo-2-oxa...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Scaglione BA,Rintoul DA

    更新日期:1989-05-01 00:00:00

  • Horizontal saccade disconjugacy in strabismic monkeys.

    abstract:PURPOSE:Previous studies have shown that binocular coordination during saccadic eye movement is affected in humans with large strabismus. The purpose of this study was to examine the conjugacy of saccadic eye movements in monkeys with sensory strabismus. METHODS:The authors recorded binocular eye movements in four str...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0955

    authors: Fu L,Tusa RJ,Mustari MJ,Das VE

    更新日期:2007-07-01 00:00:00

  • Panton-Valentine Leucocidin of Staphylococcus aureus Induces Oxidative Stress and Neurotransmitter Imbalance in a Retinal Explant Model.

    abstract:Purpose:Endophthalmitis models have reported the virulent role of Panton-Valentine leucocidin (PVL) secreted by Staphylococcus aureus on the retina. PVL targets retinal ganglion cells (RGCs), expressing PVL membrane receptor C5aR. Interactions between PVL and retinal cells lead to glial activation, retinal inflammation...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.62.1.4

    authors: Wurtz M,Ruhland E,Liu X,Namer IJ,Mazzoleni V,Lipsker D,Keller D,Prévost G,Gaucher D

    更新日期:2021-01-04 00:00:00

  • Pattern of expression of p53, its family members, and regulators during early ocular development and in the post-mitotic retina.

    abstract:PURPOSE:Because of its role in cell cycle regulation and apoptosis, p53 may be involved in maintaining the post-mitotic state of the adult eye. To shed light on the role of p53 in retinal development and maintenance, this study investigated the pattern of expression of p53, its family members, and its regulators during...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8681

    authors: Vuong L,Brobst DE,Saadi A,Ivanovic I,Al-Ubaidi MR

    更新日期:2012-07-20 00:00:00

  • Evaluation of antiproliferative agents using a cell-culture model.

    abstract::Chinese hamster ovarian (CHO) cells in culture were used to evaluate the relative antiproliferative potential of drugs. These agents have been used to improve the clinical response after glaucoma filtering surgery. The following drugs were evaluated: 5-fluorouracil (5-FU) as the benchmark, 5-fluorouridine (FUR), 5-flu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Senderoff RI,Weber PA,Smith DR,Sokoloski TD

    更新日期:1990-12-01 00:00:00

  • Muscarinic effects on cellular functions in cultured human ciliary muscle cells.

    abstract:PURPOSE:To characterize the pharmacology of the carbachol-induced changes of phospholipase C (PLC) activity and intracellular calcium concentration ([Ca2+]i) in cultured human ciliary muscle cells. METHODS:Changes in PLC activity of cultured human ciliary muscle cells were determined by production of inositol phosphat...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Matsumoto S,Yorio T,DeSantis L,Pang IH

    更新日期:1994-09-01 00:00:00

  • Photically induced experimental exophthalmos: role of Harderian and pituitary glands.

    abstract::Exposure of adult male and female albino rats to high-intensity illumination for 17.5 hr results in a marked, transient exophthalmos, which persists for approximately 48 hr after the light exposure. Upon examination of the Harderian glands in these rats, a significant glandular weight increase was observed up to 2 day...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Johnson DD,Rudeen PK,O'Steen WK

    更新日期:1979-12-01 00:00:00

  • Retinal oxygen saturation in retinitis pigmentosa and macular dystrophies in asian-Indian eyes.

    abstract:PURPOSE:To study the oxygen-saturation profiles in RP and macular dystrophies and compare them with age-matched healthy controls. METHODS:In a cross-sectional prospective study, 62 subjects with RP, 23 with macular dystrophies, and 78 controls were enrolled, and retinal oximetry was performed with the Oxymap T1 retina...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-15993

    authors: Battu R,Mohan A,Khanna A,Kumar A,Shetty R

    更新日期:2015-05-01 00:00:00

  • Prophylactic acyclovir effectively reduces herpes simplex virus type 1 reactivation after exposure of latently infected mice to ultraviolet B.

    abstract:PURPOSE:To determine the potential efficacy and anatomic sites of action of prophylactic oral acyclovir using a murine model of ultraviolet-B-induced reactivation of herpes simplex 1 keratitis. METHODS:Latent infection with herpes simplex 1 (McKrae) was established in 80 National Institutes of Health inbred strain of ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Blatt AN,Laycock KA,Brady RH,Traynor P,Krogstad DJ,Pepose JS

    更新日期:1993-11-01 00:00:00

  • The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1.

    abstract:Purpose:Diabetes leads to the downregulation of the retinal Kir4.1 channels and Müller cell dysfunction. The insulin receptor substrate-1 (IRS-1) is a critical regulator of insulin signaling in Müller cells. Circadian rhythms play an integral role in normal physiology; however, diabetes leads to a circadian dysrhythmia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.18-26045

    authors: Luo Q,Xiao Y,Alex A,Cummins TR,Bhatwadekar AD

    更新日期:2019-05-01 00:00:00

  • Influences of atmospheric pressure and temperature on intraocular pressure.

    abstract:PURPOSE:To determine whether the atmospheric pressure (ATM) change experienced during diving can induce changes in the intraocular pressure (IOP) of eyes in a normal population. METHODS:The IOP of 27 healthy volunteers (ages, 23.8 +/- 4.9 years; range, 18-44) was measured with a Perkins applanation tonometer by two in...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1578

    authors: Van de Veire S,Germonpre P,Renier C,Stalmans I,Zeyen T

    更新日期:2008-12-01 00:00:00