Cell morphology in long-term cultures of normal and abnormal amniotic fluids.

Abstract:

:The cell morphology of long-term cultures of amniotic fluid cells from 10 fetuses with a neural tube defect (NTD) and three with omphalocele was examined and compared to 30 long-term cultures of normal amniotic fluids as well as a long-term culture of human fetal brain. Cultures from the amniotic fluids of the fetuses with NTD and omphalocele showed cells with the same general characteristics as normal amniotic fluid cells. However, the cultures of amniotic fluid cells from NTD pregnancies had an additional cell type also seen in fetal brain culture. This was a neuroblast-like cell, with small rounded refractile morphology and long branching processes forming clusters of varying sizes which lay on top of large flat cells. These neuroblast-like cells diminished in number with time in culture and were not present in subcultures. Their possible neuronal origin is discussed.

journal_name

Hum Genet

journal_title

Human genetics

authors

Medina-Gómez P,Johnston TH

doi

10.1007/BF00569209

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

310-3

issue

4

eissn

0340-6717

issn

1432-1203

journal_volume

60

pub_type

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