Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen.

Abstract:

:Hybridomas secreting monoclonal antibodies against an apparent strain-specific cell surface antigen of Neisseria gonorrhoeae were produced. Spleen cells from BALB/c mice immunized with whole gonococci were fused with mouse myeloma cell line Sp2/0, and hybrid cells were selected in culture. One hybridoma that secreted antibodies reactive with the immunizing strain was cloned by limiting dilution to obtain cell lines secreting monoclonal antibodies. These antibodies reacted with purified outer membranes from the immunizing strain as well as with whole gonococci. Binding of antibodies to whole gonococci was highly strain specific, with most gonococcal strains showing less than 1% of the binding with the immunizing strain. Antibodies did not bind to the other Neisseria species tested. Binding of monoclonal antibodies to whole gonococci of the immunizing strain was not dependent on state of piliation. The extent of antibody binding did vary in different colonial variants of the immunizing strain. Antibody bound to cells from colonies that were transparent or of intermediate opacity, but did not bind to cells from deeply opaque colony variants.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Nachamkin I,Cannon JG,Mittler RS

doi

10.1128/IAI.32.2.641-648.1981

subject

Has Abstract

pub_date

1981-05-01 00:00:00

pages

641-8

issue

2

eissn

0019-9567

issn

1098-5522

journal_volume

32

pub_type

杂志文章
  • Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal but not systemic immunization.

    abstract::The purpose of the present study was to determine the extent of immunologic responses, particularly immunopathologic responses, within the upper and lower respiratory tracts after intranasal immunization using the mucosal adjuvant cholera toxin (CT). BALB/c mice were nasally immunized with influenza virus vaccine comb...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.4.2328-2338.2001

    authors: Hodge LM,Marinaro M,Jones HP,McGhee JR,Kiyono H,Simecka JW

    更新日期:2001-04-01 00:00:00

  • Interaction of Bartonella henselae with endothelial cells promotes monocyte/macrophage chemoattractant protein 1 gene expression and protein production and triggers monocyte migration.

    abstract::Bacillary angiomatosis (BA), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Macrophages have been identified as important effector cells contributing to the angiogenic process during B. henselae ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.9.5735-5742.2005

    authors: McCord AM,Burgess AW,Whaley MJ,Anderson BE

    更新日期:2005-09-01 00:00:00

  • Genetically detoxified mutants of heat-labile toxin from Escherichia coli are able to act as oral adjuvants.

    abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.9.4400-4406.1999

    authors: Douce G,Giannelli V,Pizza M,Lewis D,Everest P,Rappuoli R,Dougan G

    更新日期:1999-09-01 00:00:00

  • CsrRS and environmental pH regulate group B streptococcus adherence to human epithelial cells and extracellular matrix.

    abstract::Streptococcus agalactiae (group B Streptococcus or GBS) is a common colonizer of the gastrointestinal and genital tracts and an important cause of invasive infections in newborn infants and in adults with predisposing chronic conditions or advanced age. Attachment to epithelial surfaces at mucosal sites is a critical ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00699-12

    authors: Park SE,Jiang S,Wessels MR

    更新日期:2012-11-01 00:00:00

  • Spa contributes to the virulence of type 18 group A streptococci.

    abstract::Streptococcal protective antigen (Spa) is a newly described surface protein of group A streptococci that was recently shown to evoke protective antibodies (J. B. Dale, E. Y. Chiang, S. Liu, H. S. Courtney, and D. L. Hasty, J. Clin. Investig. 103:1261--1268, 1999). In this study, we have determined the complete sequenc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.5.2943-2949.2001

    authors: McLellan DG,Chiang EY,Courtney HS,Hasty DL,Wei SC,Hu MC,Walls MA,Bloom JJ,Dale JB

    更新日期:2001-05-01 00:00:00

  • Role of ficolin-A and lectin complement pathway in the innate defense against pathogenic Aspergillus species.

    abstract::Aspergillus species are saprophytic molds causing life-threatening invasive fungal infections in the immunocompromised host. Innate immune recognition, in particular, the mechanisms of opsonization and complement activation, has been reported to be an integral part of the defense against fungi. We have shown that the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00032-13

    authors: Bidula S,Kenawy H,Ali YM,Sexton D,Schwaeble WJ,Schelenz S

    更新日期:2013-05-01 00:00:00

  • Acquired immunity in experimental murine aspergillosis is mediated by macrophages.

    abstract::A number of studies have substantiated the pivotal role of innate defense mechanisms in protection against invasive aspergillosis. However, experiments demonstrating increased resistance to lethal intravenous (i.v.) infection with Aspergillus fumigatus conidia in cortisone-treated or untreated mice preinfected with a ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.9.3791-3802.1993

    authors: de Repentigny L,Petitbois S,Boushira M,Michaliszyn E,Sénéchal S,Gendron N,Montplaisir S

    更新日期:1993-09-01 00:00:00

  • Susceptibility to infectious diseases based on antimicrobial peptide production.

    abstract::In the last few years, the great impact of antimicrobial peptides on infectious disease susceptibility and natural resistance has been reported. In some cases, susceptibility to diseases is related to antimicrobial peptide polymorphisms and gene copy numbers, but for the vast majority of infectious diseases, these phe...

    journal_title:Infection and immunity

    pub_type: 杂志文章,评审

    doi:10.1128/IAI.01515-08

    authors: Rivas-Santiago B,Serrano CJ,Enciso-Moreno JA

    更新日期:2009-11-01 00:00:00

  • Role of the surface lipoprotein BBA07 in the enzootic cycle of Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lip...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00372-10

    authors: Xu H,He M,He JJ,Yang XF

    更新日期:2010-07-01 00:00:00

  • Campylobacter-Wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium.

    abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.5.1380-1383.1989

    authors: Wyss C

    更新日期:1989-05-01 00:00:00

  • Mucinophilic and chemotactic properties of Pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis.

    abstract::Representative isolates of nonmucoid Pseudomonas aeruginosa were studied to investigate the hypothesis that mucinophilic and chemotactic properties in this species act as potential factors in the initial stages of pulmonary colonization in patients with cystic fibrosis (CF). Transmission electron microscopy with a sur...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.6.1489-1495.1990

    authors: Nelson JW,Tredgett MW,Sheehan JK,Thornton DJ,Notman D,Govan JR

    更新日期:1990-06-01 00:00:00

  • Contribution of choline-binding proteins to cell surface properties of Streptococcus pneumoniae.

    abstract::Nonspecific interactions related to physicochemical properties of bacterial cell surfaces, such as hydrophobicity and electrostatic charge, are known to have important roles in bacterium-host cell encounters. Streptococcus pneumoniae (pneumococcus) expresses multiple, surface-exposed, choline-binding proteins (CBPs) w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.1.412-415.2002

    authors: Swiatlo E,Champlin FR,Holman SC,Wilson WW,Watt JM

    更新日期:2002-01-01 00:00:00

  • Experimental model for dermal granulomatous hypersensitivity in Q fever.

    abstract::Q fever has been associated with granulomatous changes in clinical biopsy material obtained from liver and bone marrow. Local reactions to skin testing have been described in previously sensitized humans, but histological studies of such reactions have not been reported. We note that delayed hypersensitivity reactions...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.39.1.388-393.1983

    authors: Ascher MS,Berman MA,Parker D,Turk JL

    更新日期:1983-01-01 00:00:00

  • Propagation of infantile gastroenteritis virus (orbi-group) in conventional and germfree piglets.

    abstract::Infantile gastroenteritis virus (orbi-group) recovered from stools of infants with acute nonbacterial gastroenteritis was administered per os to germfree and conventional piglets. Virus was found subsequently in stools and in the mucosal epithelial cells of the small intestine of these animals. Some animals developed ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.12.6.1276-1280.1975

    authors: Middleton PJ,Petric M,Szymanski MT

    更新日期:1975-12-01 00:00:00

  • The fibronectin-binding proteins of Staphylococcus aureus may promote mammary gland colonization in a lactating mouse model of mastitis.

    abstract::The fibronectin-binding proteins (FnBPs) of Staphylococcus aureus are believed to be implicated in the pathogen's adherence to and colonization of bovine mammary glands, thus leading to infectious mastitis. In vitro studies have shown that FnBPs help the adhesion of the pathogen to bovine mammary epithelial cells. How...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.4.2292-2295.2003

    authors: Brouillette E,Talbot BG,Malouin F

    更新日期:2003-04-01 00:00:00

  • Surface-expressed mig protein protects Streptococcus dysgalactiae against phagocytosis by bovine neutrophils.

    abstract::The mig gene of Streptococcus dysgalactiae, a major bovine mastitis pathogen, encodes two plasma protein-binding receptors, alpha2-macroglobulin (alpha2-M) and immunoglobulin G (IgG). In this study, the mig gene from one S. dysgalactiae isolate was cloned and expressed in Escherichia coli. The IgG receptor region enco...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.10.6030-6037.2001

    authors: Song XM,Perez-Casal J,Bolton A,Potter AA

    更新日期:2001-10-01 00:00:00

  • Mice immunized by primary vaginal Candida albicans infection develop acquired vaginal mucosal immunity.

    abstract::It has been postulated that systemic cell-mediated immunity (CMI) is an important host defense mechanism against Candida infections of the vagina. However, in an estrogen-dependent murine model of experimental vaginal candidiasis, we recently showed that systemic Candida-specific Th1-type CMI induced by immunization w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.2.547-553.1995

    authors: Fidel PL Jr,Lynch ME,Conaway DH,Tait L,Sobel JD

    更新日期:1995-02-01 00:00:00

  • Mapping of bactericidal epitopes on the P2 porin protein of nontypeable Haemophilus influenzae.

    abstract::The P2 porin protein is the major outer membrane protein of nontypeable Haemophilus influenzae and is a potential target of a protective immune response. Nine monoclonal antibodies (MAbs) to P2 were developed by immunizing mice with nontypeable H. influenzae whole organisms. Each MAb reacted exclusively with the homol...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.9.3712-3722.1994

    authors: Haase EM,Yi K,Morse GD,Murphy TF

    更新日期:1994-09-01 00:00:00

  • Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella pneumophila revealed by coinfection.

    abstract::Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niche...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.8.4494-4504.2005

    authors: Sauer JD,Shannon JG,Howe D,Hayes SF,Swanson MS,Heinzen RA

    更新日期:2005-08-01 00:00:00

  • Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis.

    abstract::To assess the humoral immunological responses at the subclass level in shigellosis, specific antibody responses against Shigella dysenteriae 1 lipopolysaccharide (LPS), S. flexneri Y LPS, invasion plasmid-coded protein antigens (Ipa), and Shiga toxin were analyzed. Antibody responses of 41 patients with S. dysenteriae...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.5.2054-2061.1995

    authors: Islam D,Wretlind B,Ryd M,Lindberg AA,Christensson B

    更新日期:1995-05-01 00:00:00

  • Human lactoferrin and peptides derived from a surface-exposed helical region reduce experimental Escherichia coli urinary tract infection in mice.

    abstract::Lactoferrin (LF) is a multifunctional immunoregulatory protein that has been associated with host defense at mucosal surfaces through its antibacterial properties. The antibacterial and anti-inflammatory properties of LF were further explored with an animal model of experimental urinary tract infection. Bovine LF (bLF...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.10.5816-5823.2000

    authors: Håversen LA,Engberg I,Baltzer L,Dolphin G,Hanson LA,Mattsby-Baltzer I

    更新日期:2000-10-01 00:00:00

  • Clostridium perfringens enterotoxin damages the human intestine in vitro.

    abstract::In vitro, Clostridium perfringens enterotoxin (CPE) binds to human ileal epithelium and induces morphological damage concurrently with reduced short-circuit current, transepithelial resistance, and net water absorption. CPE also binds to the human colon in vitro but causes only slight morphological and transport chang...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.12.8407-8410.2005

    authors: Fernández Miyakawa ME,Pistone Creydt V,Uzal FA,McClane BA,Ibarra C

    更新日期:2005-12-01 00:00:00

  • Mitogenic stimulation of murine spleen cells: relation to susceptibility to Salmonella infection.

    abstract::The screening of several inbred strains of mice suggested that the capacity of their spleen cells to respond to the mitogenic effect of lipopolysaccharide (LPS) of gram-negative bacteria was correlated with their resistance to intraperitoneal infection with Salmonella typhimurium. An infection of LPS into mice caused ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.1.26-33.1977

    authors: von Jeney N,Günther E,Jann K

    更新日期:1977-01-01 00:00:00

  • Parasitophorous vacuoles of Leishmania amazonensis-infected macrophages maintain an acidic pH.

    abstract::Leishmania amastigotes are intracellular protozoan parasites of mononuclear phagocytes which reside within parasitophorous vacuoles of phagolysosomal origin. The pH of these compartments was studied with the aim of elucidating strategies used by these microorganisms to evade the microbicidal mechanisms of their host c...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.3.779-787.1990

    authors: Antoine JC,Prina E,Jouanne C,Bongrand P

    更新日期:1990-03-01 00:00:00

  • Impact of methoxymycolic acid production by Mycobacterium bovis BCG vaccines.

    abstract::BCG vaccines are a family of closely related daughter strains of an attenuated isolate of Mycobacterium bovis derived by in vitro passage from 1908 to 1921. During subsequent laboratory propagation of the vaccine strain until its lyophilization in 1961, BCG Pasteur underwent at least seven further genomic mutations. T...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.5.2803-2809.2004

    authors: Belley A,Alexander D,Di Pietrantonio T,Girard M,Jones J,Schurr E,Liu J,Sherman DR,Behr MA

    更新日期:2004-05-01 00:00:00

  • Involvement of cells of hematopoietic origin in genetically determined resistance of Syrian hamsters to vesicular stomatitis virus.

    abstract::Susceptibility of Syrian hamsters of the inbred LSH and MHA strains to injection of as few as 10 plaque-forming units of vesicular stomatitis virus (VSV) was shown to occur only after intraperitoneal and intrapleural injection and not after injection of VSV intravenously, intranasally, or in the footpads. Despite the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.34.2.540-549.1981

    authors: Fultz PN,Shadduck JA,Kang CY,Streilein JW

    更新日期:1981-11-01 00:00:00

  • Murine neonates infected with Yersinia enterocolitica develop rapid and robust proinflammatory responses in intestinal lymphoid tissues.

    abstract::Neonatal animals are generally very susceptible to infection with bacterial pathogens. However, we recently reported that neonatal mice are highly resistant to orogastric infection with Yersinia enterocolitica. Here, we show that proinflammatory responses greatly exceeding those in adults arise very rapidly in the mes...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01489-13

    authors: Siefker DT,Echeverry A,Brambilla R,Fukata M,Schesser K,Adkins B

    更新日期:2014-02-01 00:00:00

  • A peptide permease mutant of Mycobacterium bovis BCG resistant to the toxic peptides glutathione and S-nitrosoglutathione.

    abstract::Oligopeptides play important roles in bacterial nutrition and signaling. Using sequences from the available genome database for Mycobacterium tuberculosis H37Rv, the oligopeptide permease operon (oppBCDA) of Mycobacterium bovis BCG was cloned from a cosmid library. An opp mutant strain was constructed by homologous re...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.2.429-436.2000

    authors: Green RM,Seth A,Connell ND

    更新日期:2000-02-01 00:00:00

  • Bacteriophage Mu d1(Apr lac) generates vir-lac operon fusions in Shigella flexneri 2a.

    abstract::Previous studies have demonstrated that expression of virulence in Shigella spp. is controlled by growth temperature. To study the regulation of virulence (vir) genes, we set out to develop a rapid, easily-assayed phenotype with which to measure expression of virulence. This report described a procedure for isolating ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.45.3.642-648.1984

    authors: Maurelli AT,Curtiss R 3rd

    更新日期:1984-09-01 00:00:00

  • Characterization of a Chlamydia psittaci DNA binding protein (EUO) synthesized during the early and middle phases of the developmental cycle.

    abstract::The EUO gene (for early upstream open reading frame) of Chlamydia psittaci was previously found to be transcribed better at 1 than at 24 h postinfection. We found that the EUO gene encodes a minor protein that is expressed within 1 h of infection of host cells with C. psittaci 6BC but that protein quantity peaks durin...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.3.1167-1173.1998

    authors: Zhang L,Douglas AL,Hatch TP

    更新日期:1998-03-01 00:00:00