Immunoperoxidase procedures to detect monoclonal antibodies against cell surface antigens. Quantitation of binding and staining of individual cells.

Abstract:

:An immunoperoxidase method has been developed which allows accurate and sensitive quantitation of the binding of monoclonal antibodies to cell surface antigens. Monolayers of fixed cells were prepared in wells of Terasaki micro-test plates and monoclonal antibodies bound to cell surface antigens were identified by the unlabeled antibody-enzyme method of Sternberger (1974). The cell-bound peroxidase could either be quantified per well or visualized on individual cells by the use of appropriate substrates for peroxidase. Experimental procedures are described in detail and results obtained with several monoclonal antibodies with specificity for different target cells are shown. Limitations and applications of the technique are discussed.

journal_name

J Immunol Methods

authors

Lansdorp PM,Astaldi GC,Oosterhof F,Janssen MC,Zeijlemaker WP

doi

10.1016/0022-1759(80)90240-9

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

393-405

issue

4

eissn

0022-1759

issn

1872-7905

pii

0022-1759(80)90240-9

journal_volume

39

pub_type

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