Abstract：
:The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Marcus SL,Modak MJdoi
10.1093/nar/3.6.1473subject
Has Abstractpub_date
1976-06-01 00:00:00pages
1473-86issue
6eissn
0305-1048issn
1362-4962journal_volume
3pub_type
杂志文章abstract：:The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was esta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.11.3271
更新日期：1990-06-11 00:00:00
abstract：:The mRNAs for different variant surface antigens of Trypanosoma brucei start with the same 35 nucleotides. This sequence is encoded by a separate mini-exon, located in a 1.35-kb repetitive element. We have reported that trypanosomes contain many transcripts that hybridize to mini-exon probes, even if they do not make ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.3777
更新日期：1984-05-11 00:00:00
abstract：:In order to facilitate the direct computation of the sizes of DNA fragments separated by gel electrophoresis, we have written and evaluated programmes for the Hewlett-Packard 41C programmable calculator. The sizes estimated for DNA fragments of known length using some of these programmes were found to be more accurate...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.1part2.845
更新日期：1984-01-11 00:00:00
abstract：:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.1.19
更新日期：1989-01-11 00:00:00
abstract：:Identification of components essential to chromosome structure and behaviour remains a vibrant area of study. We have previously shown that invadolysin is essential in Drosophila, with roles in cell division and cell migration. Mitotic chromosomes are hypercondensed in length, but display an aberrant fuzzy appearance....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv211
更新日期：2015-04-20 00:00:00
abstract：:The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq318
更新日期：2010-07-01 00:00:00
abstract：:Four hundred twenty-eight high-resolution DNA-protein complexes were chosen for a bioinformatics study. Although 164 crystal structures (38% of those searched) contained no interactions, 574 discrete π-contacts between the aromatic amino acids and the DNA nucleobases or deoxyribose were identified using strict criteri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku269
更新日期：2014-06-01 00:00:00
abstract：:The PAH Mutation Analysis Consortium (81 investigators, 26 countries) is engaged in mutation detection at the human PAH locus. Ascertainment of probands occurs largely through newborn screening for hyperphenylalaninemia. A relational database records allelic variation (disease-producing and polymorphic) at the locus. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.1.127
更新日期：1996-01-01 00:00:00
abstract：:Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers. We recently described serine/arginine-rich splicing factor 3 (SRSF3 or SRp20) being a proto-oncogene. However, the SRSF3-regulated splic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1500
更新日期：2016-02-29 00:00:00
abstract：:Despite the increasing wealth of available data, the structure of cancer transcriptional space remains largely unknown. Analysis of this space would provide novel insights into the complexity of cancer, assess relative implications in complex biological processes and responses, evaluate the effectiveness of cancer mod...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr533
更新日期：2011-10-01 00:00:00
abstract：:We describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously expressed Rosa26 locus. Subsequent regulation of gene activity is governed by a dual regulatory...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni146
更新日期：2005-10-12 00:00:00
abstract：:High performance liquid chromatography analysis of imidazole open ring 7-methylguanine, 2-6 diamino-4-hydroxy-5N-methyl-formamidopyrimidine (rom7G), showed two well-separated peaks (fI and fII) of the same magnitude. Rechromatography of each isolated component indicated that they are slowly interconverted to give a 1:...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.13.5429
更新日期：1984-07-11 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) play critical roles in diverse cellular processes; however, their involvement in many critical aspects of the immune response including the interferon (IFN) response remains poorly understood. To address this gap, we compared the global gene expression pattern of primary human hepatocyte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku713
更新日期：2014-01-01 00:00:00
abstract：:The Chemical Effects in Biological Systems database (CEBS) is a comprehensive and unique toxicology resource that compiles individual and summary animal data from the National Toxicology Program (NTP) testing program and other depositors into a single electronic repository. CEBS has undergone significant updates in re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1077
更新日期：2017-01-04 00:00:00
abstract：:The Network of Cancer Genes (NCG) collects and integrates data on 736 human genes that are mutated in various types of cancer. For each gene, NCG provides information on duplicability, orthology, evolutionary appearance and topological properties of the encoded protein in a comprehensive version of the human protein-p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp957
更新日期：2010-01-01 00:00:00
abstract：:COMPASS is a method for homology detection and local alignment construction based on the comparison of multiple sequence alignments (MSAs). The method derives numerical profiles from given MSAs, constructs local profile-profile alignments and analytically estimates E-values for the detected similarities. Until now, CO...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm293
更新日期：2007-07-01 00:00:00
abstract：:Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the resp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.18.3653
更新日期：1996-09-15 00:00:00
abstract：:We have previously described programs for a variety of types of sequence analysis (1-4). These programs have now been integrated into a single package. They are written in the standard C programming language and run on virtually any computer system with a C compiler, such as the IBM/PC and other computers running unde...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.1.443
更新日期：1986-01-10 00:00:00
abstract：:Unlike most yeast ribosomal protein genes studied so far the genes coding for S24 and L46 are adjacent on the genome. Sequence analysis showed that the two genes are transcribed divergently, their initiation codons being 630 bp apart. Taking the respective ATG translation start sites as reference points, the 5'- end o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.3.701
更新日期：1985-02-11 00:00:00
abstract：:Using a combination of mathematical modelling, statistical simulation and large-scale data analysis we study the properties of linear regulatory chains (LRCs) within gene regulatory networks (GRNs). Our modelling indicates that downstream genes embedded within LRCs are highly insulated from the variation in expression...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx485
更新日期：2017-07-07 00:00:00
abstract：:We searched for nucleotide excision repair in human cell-free extracts using two assays: damage-specific incision of DNA (the nicking assay) and damage-stimulated DNA synthesis (the repair synthesis assay). HeLa cell-free extract prepared by the method of Manley et al. (1980) has a weak nicking activity on UV irradiat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.12.4471
更新日期：1989-06-26 00:00:00
abstract：:The Structural Classification of Proteins (SCOP) database is a comprehensive ordering of all proteins of known structure, according to their evolutionary and structural relationships. Protein domains in SCOP are hierarchically classified into families, superfamilies, folds and classes. The continual accumulation of se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh039
更新日期：2004-01-01 00:00:00
abstract：:From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed through an internal promoter. And the L1 internal promoter, unlike other internal promoters, is thought to be RNA polymerase I...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.18.3704
更新日期：1995-09-25 00:00:00
abstract：:A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data, especially those obtained by the sequencing method using M13 phages. Programs NUCDAT and PROTEN are for analyzing DNA/RNA and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.1part1.101
更新日期：1984-01-11 00:00:00
abstract：:Twenty AdoMet-dependent methyltransferases (MTases) have been characterized structurally by X-ray crystallography and NMR. These include seven DNA MTases, five RNA MTases, four protein MTases and four small molecule MTases acting on the carbon, oxygen or nitrogen atoms of their substrates. The MTases share a common co...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/29.18.3784
更新日期：2001-09-15 00:00:00
abstract：:During erythropoiesis in the chick, the nucleosome repeat length increases from 190 base pairs to 212 base pairs. This increase is correlated with a dramatic increase in the concentration of the red cell specific histone H5 (from 0.2 molecules per nucleosome to 1 molecule per nucleosome) and with no change in the conc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.4.1179
更新日期：1978-04-01 00:00:00
abstract：:The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku622
更新日期：2014-08-01 00:00:00
abstract：:In spermidine-condensed calf thymus DNA preparations, torus-shaped condensates were shown by transmission electron microscopy to exist under the hydrated conditions of the freeze fracture experiment. Using extremely low Pt metal deposition levels (9 A Pt/C) high-contrast replicas of the spermidine-DNA toruses were obt...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.6.1839
更新日期：1983-03-25 00:00:00
abstract：:The 5' portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes h...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.10.4667
更新日期：1988-05-25 00:00:00
abstract：:Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.3050
更新日期：1995-08-11 00:00:00
