Abstract：
:Interaction of cationized ferritin with the anionic groups on the cell surface of Naegleria was studied using transmission electron microscopy in conjunction with fluorescence microscopy. Most of the experiments involved the use of fluorescein-labelled cationized ferritin (FITC-CF) incubated with living amoebae. Initially the FITC-CF was located over the posterior two-thirds of the amoebae but the label was rapidly redistributed to form a cap at the posterior end in the region of the uroid; frequently this cap was shed. Pinosomes containing FITC-CF were clearly visible within the amoebae. Amoebae prefixed in glutaraldehyde were uniformly stained and did not show redistribution of the label. Exposure of live amoebae, previously incubated with cationized ferritin and allowed to cap, to fresh FITC-CF failed to produce fluorescent staining of the general cell surface, i.e. depletion of binding sites had occurred. The binding of the FITC-CF was not affected by pretreatment of the amoebae with neuraminidase or pronase. The possible nature of the anionic sites on the membrane is discussed.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
King CA,Preston TMsubject
Has Abstractpub_date
1977-12-01 00:00:00pages
133-49eissn
0021-9533issn
1477-9137journal_volume
28pub_type
杂志文章abstract：:Ena/VASP proteins are actin-binding proteins that localize to actin stress fibres, the tips of filopodia and the lamellipodial leading edge. In the past few years, a number of seemingly conflicting studies have confused the Ena/VASP field, pointing to roles for these proteins in both promotion and inhibition of actin-...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.00218
更新日期：2002-12-15 00:00:00
abstract：:Human peripheral blood eosinophils attach to and flatten down onto antibody-coated surfaces and subsequently degranulate. An antibody-coated surface was prepared by treating a layer of agar, containing tetanus toxoid antigen and eosinophil chemotactic factor (ECF), with human anti-tetanus immunoglobin. Changes in eosi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:During gestation the epidermis develops from a single layer of ectoderm into a layer of keratinocytes overlaid by a layer of periderm; this is followed by a progressive increase in the number of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are for...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:The complex lipid constituents of the eukaryotic plasma membrane are precisely controlled in a cell-type-specific manner, suggesting an important, but as yet, unknown cellular function. Neuronal membranes are enriched in long-chain polyunsaturated fatty acids (LC-PUFAs) and alterations in LC-PUFA metabolism cause debi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00918
更新日期：2003-12-15 00:00:00
abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01734
更新日期：2005-04-01 00:00:00
abstract：:In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:The effects of hyaluronate on the morphology, motile and social behaviour of embryonic chick heart fibroblasts were examined in the presence or absence of hyaluronate binding proteins (HABP). HABP were inserted onto fibroblasts after treating cells with 0.2 M-urea. Such treatment increased the binding of [125I]HABP to...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:Annexin 1 is a Ca(2+)-regulated membrane binding protein and a major substrate of the epidermal growth factor receptor kinase. Because of its properties and intracellular distribution, the protein has been implicated in endocytic trafficking of the receptor, in particular in receptor sorting occurring in multivesicula...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:During the prometaphase stage of mitosis, the cell builds a bipolar spindle of microtubules that mechanically segregates sister chromatids between two daughter cells in anaphase. The spindle assembly checkpoint (SAC) is a quality control mechanism that monitors proper attachment of microtubules to chromosome kinetocho...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.191353
更新日期：2018-04-12 00:00:00
abstract：:Cultured human erythrocytes infected with Plasmodium falciparum were studied by freeze-fracture electron microscopy. Special emphasis was placed upon the formation of the membrane surface excrescences ('knobs') found on red cells containing mature parasites. Knobs were visualized as conoid projections of the protoplas...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Integrin alpha1beta1 is a collagen receptor predominantly found in mesenchymal tissues. Mice lacking this receptor are viable. We have previously suggested that alpha1beta1 might participate in the down-regulation of collagen gene expression observed in cells suspended inside collagen gels. The results presented here ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.138313
更新日期：2013-11-15 00:00:00
abstract：:The yeast nuclear scaffold has been shown to bind with high affinity to genomic sequences that permit autonomous DNA replication of plasmids (ARS elements). We describe here conditions for the isolation of a histone-free nuclear substructure, the nuclear scaffold, from Saccharomyces cerevisiae. We examine the protein ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell-cell contact and transcriptional regulation of proliferation, coordinating epithelial tissue homeostasis and regeneration. The integration of multiple growth-regulatory inputs with ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.150888
更新日期：2014-07-01 00:00:00
abstract：:Guinea pig sperm were suspended in calcium-containing medium supplemented with various concentrations of the tetrasaccharide, stachyose. At concentrations up to and including 0.6 M, stachyose was without effect on the A23187-induced acrosome reaction. At 1.0 M stachyose, greater than 97% of sperm retained their acroso...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:The IGF-II mRNA-binding proteins (IMPs), which are composed of two RNA recognition motifs, (RRM) and four hnRNP K homology (KH) domains, have been implicated in subcytoplasmic localization of mRNAs during embryogenesis. The IMP family originated via two gene duplications before the divergence of vertebrates, and IMP h...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-05-15 00:00:00
abstract：:Start is the main decision point in the eukaryotic cell cycle at which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional programme through the inactivation of Start transcriptional repressors: the retinoblastoma family in mammals, or Whi5 and its recently identi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.251413
更新日期：2020-12-21 00:00:00
abstract：:During development, axons migrate long distances in responses to attractive or repulsive signals that are detected by their growth cones. One of these signals is mediated by netrin-1, a diffusible laminin-related molecule that both attracts and repels growth cones via interaction with its receptor DCC (deleted in colo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02296
更新日期：2005-04-15 00:00:00
abstract：:We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quanti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in the growth cone. However, despite the relatively large number of proteins implicated in actin-microtubule crosstalk, the mechanisms whereby actin polymerization is coupled to microtubule stabilization and advancement in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.203455
更新日期：2017-08-01 00:00:00
abstract：:Protrusion formation is the first step that precedes cell movement of motile cells. Spatial control of actin polymerization is necessary to achieve directional protrusion during cell migration. Here we show that the spatial coordinators p190RhoGEF and p190RhoGAP regulate actin polymerization during leading edge protru...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.123547
更新日期：2013-08-01 00:00:00
abstract：:We investigated the in vivo localisation of fission yeast cyclin-dependent kinase cdc2p during mitosis and meiosis. Fusion to yellow fluorescent protein (YFP) revealed that cdc2-YFP is present in the cytoplasm at all stages of the cell cycle. Nuclear cdc2-YFP fluorescence oscillates with that of cdc13-YFP cyclin. At G...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of expression. We have observed that transduced connexin32, connexin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00442
更新日期：2003-06-01 00:00:00
abstract：:In some individuals with autosomal dominant isolated growth hormone deficiency, one copy of growth hormone lacks amino acids 32-71 and is severely misfolded. We transfected COS7 cells with either wild-type human growth hormone or Delta 32-71 growth hormone and investigated subcellular localization of growth hormone an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:The path that RNA takes through nuclear pores was mapped using two high-resolution techniques. Unexpectedly, no RNA in HL60 cells was detected by immunogold labelling in the central axis of the pore complex on its way to the transporter at the nuclear membrane; instead, it was distributed around the sides, apparently ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Integrins are implicated in cell adhesion, migration and homeostasis. An important feature is their ability to adopt different affinity states that can be regulated by a variety of intra- and extracellular factors. To study affinity modulation of the integrin ectodomain by extracellular factors, we produced a soluble ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:The Na(+)/H(+) exchanger NHE1 is involved in intracellular pH homeostasis and cell volume regulation and accumulates with actin in the lamellipodia of fibroblasts. In order to determine the role of NHE1 following epithelial transformation and the acquisition of motile and invasive properties, we studied NHE1 expressio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Cornichon proteins are structurally related transmembrane proteins that have been studied in and Drosophila and yeast. In Drosophila, Cornichon (Cni) is involved in embryo polarization by the TGFalpha-related Gurken. In yeast, the Cni-related Erv14 is required for axial budding. A cargo receptor function has been prop...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.004200
更新日期：2007-07-15 00:00:00
abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.022103
更新日期：2008-04-01 00:00:00
abstract：:A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were collected of bovine aortic endothelial cells (BAEC) plated onto glas...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
