Anaerobic transport in Escherichia coli membrane vesicles.

Abstract:

:Anaerobic beta-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of alpha-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic beta-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport in vitro. Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.

authors

Konings WN,Kaback HR

doi

10.1073/pnas.70.12.3376

subject

Has Abstract

pub_date

1973-12-01 00:00:00

pages

3376-81

issue

12

eissn

0027-8424

issn

1091-6490

journal_volume

70

pub_type

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